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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for Zinc Finger Protein 50 ZNF50 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 50 μg total protein was run on NuPage 4-12% Bis-Tris polyacrylamide gels (Life Technologies) and transferred to nitrocellulose membranes ...
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bioRxiv - Neuroscience 2023Quote: ... followed by the addition of 50 μl of protein G-agarose beads (Thermo Scientific, Cat#20397). Immunoprecipitated proteins were recovered by centrifugation at 2,500× rpm for 2 min ...
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bioRxiv - Genetics 2021Quote: The coding sequence for the pH insensitive Citrine fluorescent protein was inserted in the coding sequence of the BK2L3 protein (Zm00001d034049) immediately after the N-terminal secretory peptide domain and synthesized in the pDONOR221 vector (Life Technologies, USA) (Tian et al. ...
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bioRxiv - Neuroscience 2019Quote: Each tryptic peptide mixture obtained from 150 μg of protein extract was isotopically labeled with the corresponding TMT6plex reagent (Thermo Fisher Sci.) based on the standard procedure ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were sequenced by Edman Degradation using a Perkin Elmer 494 Procise Protein/Peptide Sequencer with an on-line 140C PTH Amino Acid Analyzer (Applied Biosystems, Inc).
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bioRxiv - Immunology 2024Quote: ... A polyclonal antibody against the ROTR-1 protein was raised using the peptide LDRSPPSDDGTQKV (ROTR-1 amino acids 288 to 301) in rabbit (Thermo Fisher Scientific). Anti-ROTR-1 sera was used at a dilution of 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... The concentration of peptides was determined by measuring UV absorbance at 280 nm or by performing Pierce rapid gold BCA protein assay (Thermo Fisher Scientific) in case of peptides lacking aromatic amino acids ...
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bioRxiv - Microbiology 2023Quote: ... was added to cultures to label new peptide synthesis before harvesting (Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit, Invitrogen)88 ...
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bioRxiv - Bioengineering 2023Quote: ... The concentration of peptide conjugates was determined by the standard Bicinchoninic acid (BCA) assay using Pierce(tm) BCA Protein Assay Kit (Thermo Fisher Scientific). To confirm successful synthesis of peptide conjugates ...
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bioRxiv - Microbiology 2023Quote: ... Peptide sequences (and hence protein identity) were determined by comparing the fragmentation pattern acquired by the software program Sequest (Thermo Fisher Scientific) (64 ...
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bioRxiv - Biochemistry 2020Quote: Pooled peptides were fractionated by a high-pH reversed-phase peptide fractionation kit (84868, Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The digested peptides were fractionated using a high-pH reversed phase (RP) peptide fractionation kit (Thermo Scientific). Fractions collected from the high-pH RP fractionation were further separated using on-line second-dimension low-pH reversed-phase chromatography via an Easy-nLC 1200 nanoflow-UHPLC system (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: Pooled peptides were fractionated using a high-pH reversed-phase peptide fractionation kit (84868, Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Peptide concentration was quantified with a plate reader using the Pierce Quantitative Colorimetric Peptide Assay (Thermo Scientific). Finally ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 25 fmol/µl standard peptide mixture (Pierce™ Peptide Retention Time Calibration Mixture, ThermoFisher, 88321) and diluted to a final volume of 23 µl with water ...
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bioRxiv - Microbiology 2024Quote: ... 0.1% TFA and peptide content was estimated with a Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... The concentration of the peptides was determined using Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Cat#23275). Tandem mass tag (TMT)-based labeling of peptides was performed using the TMT10plex™ Isobaric Label Reagent Set (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... The peptide concentration was measured using the Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Scientific cat. # 23275). The digested peptides were subjected to simple C18 clean up using a C18 disk plug (3M Empore C18 ...
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bioRxiv - Neuroscience 2023Quote: ... desalted peptides was separated into 9 fractions with the high pH reversed-phase peptide fractionation kit (ThermoFisher). Fractions with increasing percentages of acetonitrile (ACN ...
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bioRxiv - Microbiology 2023Quote: ... the concentration of peptides was measured using Pierce Quantitative fluorometric peptide assay (ThermoFisher Scientific, Rockford, IL, USA).The resulting peptides were sequenced on a Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The peptide concentration in the supernatant was quantified using a quantitative colorimetric peptide assay from Thermo Fisher Scientific (Cat ...
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bioRxiv - Cell Biology 2023Quote: ... and the supernatant was recovered for peptide desalting using Pierce™ Peptide Desalting Spin Column (ThermoFisher: 89851) as described by the manufacturer ...
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bioRxiv - Physiology 2024Quote: ... peptides were reconstituted in 0.07% trifluoroacetic acid and quantified by Fluorometric Peptide Assay (Thermo Fisher Scientific, 23290).
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bioRxiv - Molecular Biology 2024Quote: The TMT labeled peptides were fractionated using high pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... and the peptides were separated using the High pH Reversed-Phase Peptide Separation Kit (Thermo Fisher Scientific) according to the instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... were used following biotin blocking of tissues samples using endogenous Biotin-blocking kit (Life Technologies Corporation/Thermo fisher, E21390). For Aza-peptide preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... were used following biotin blocking of tissues samples using endogenous Biotin-blocking kit (Life Technologies Corporation/Thermo fisher, E21390). For Aza-peptide preparation ...
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bioRxiv - Cell Biology 2020Quote: ... and 1X PBS) before blocking for one hour with blocking buffer (Bovine Serum Albumin (Fisher Scientific, Fair Lawn, NJ), Normal Donkey Serum (Jackson ImmunoResearch Inc. ...
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bioRxiv - Physiology 2022Quote: ... Non-specific antigens were blocked for 60 min using a blocking buffer (Sea Block Blocking Buffer; ThermoFisher Scientific, USA). Then ...
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bioRxiv - Biochemistry 2020Quote: ... and with or without CLASPIN pS945 peptide (MEELLNLC(pS)GKFTSQ) (Peptide Protein Research Ltd, Bishops Waltham, UK) at different concentrations in a Thermoscientific 96 well UV microplate (Fisher scientific, Loughborough, UK). The plates were incubated at 25 °C and absorbance at 340 nm was measured in a POLARstar Omega multimode microplate reader (BMG Labtech GmbH ...
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bioRxiv - Microbiology 2021Quote: LC-MS/SM analysis of trypsin-digested proteins (peptides) was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Peptide sequences were determined by matching protein databases with the acquired fragmentation pattern by the Sequest software program (Thermo Fisher Scientific, Waltham, MA) 57 ...
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bioRxiv - Cell Biology 2023Quote: ... Concentrations of proteins and peptides were calculated from the UV absorption at 280 nm using a Nanodrop spectrophotometer (Thermo Fisher Scientific, DE; USA).
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bioRxiv - Neuroscience 2023Quote: Slices were stained for green fluorescent protein (GFP) with a rabbit monoclonal anti-GFP antibody (G10362, Molecular Probes, 1:1000 dilution in blocking buffer) and for tyrosine hydroxylase (TH ...
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bioRxiv - Immunology 2021Quote: ... Protein secretion levels in culture supernatant were quantified via ELISA for human IgG (Invitrogen, 50-112-8849) and human BAFF (R&D Systems ...
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bioRxiv - Cell Biology 2022Quote: ... Approximately 50 μg of total protein content was run on 4-12% gradient SDS-PAGE gels (Invitrogen) and transferred to Nitrocellulose membranes for 90 minutes at 80 V ...
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bioRxiv - Neuroscience 2022Quote: ... Fractions were run on SDS-PAGE and stained with InstaBlue protein stain (50-190-5499, Fisher Scientific) to select fractions that were highly enriched in αSyn ...
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bioRxiv - Microbiology 2020Quote: ... IP’s were performed using 15 μg of each antibody with 50 μL Protein G Dynabeads (ThermoFisher Scientific), for 8 hours at 4°C on rotation ...
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bioRxiv - Genomics 2019Quote: 50 μg of protein extracts were separated on a NuPAGE™ 4-12% Bis-Tris gel (Invitrogen) and then transferred to a nitrocellulose membrane using an iBlot™ Dry Blotting System (Invitrogen) ...
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bioRxiv - Molecular Biology 2019Quote: ... Immunoprecipitation was performed at 4°C for 3 hr with 50 µl protein G dynabeads (Thermo Fisher). Beads were washed five times with ice-cold Wash buffer 1 (50 mM Hepes-KOH (pH7.6) ...
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bioRxiv - Developmental Biology 2020Quote: ... Lysates were pre-cleared with 30 μl of a 50% slurry of protein A (Invitrogen, #15918-014) and protein G agarose beads (Invitrogen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Equal amounts of protein (50 ug) were separated on SDS-polyacrylamide gel (Thermo Fisher Scientific, CATCAT# EA0378BOX) and transferred onto nitrocellulose membranes (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were pre-cleared with 30 μl of a 50% slurry of protein A (Invitrogen, #15918-014) and protein G agarose beads (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... and then 1:50 with BCA assay reagent (Pierce™ BCA Protein Assay Kit, Thermo Fisher Scientific) in a 96-microtiter plate in triplicate ...
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bioRxiv - Neuroscience 2022Quote: ... 400µL of RNA wash from SurePrep RNA/DNA/Protein Purification Kit (Fisher Scientific, Catalog No. BP2802-50) was added to the RNeasy column and spun at the same setting for 1 minute ...
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bioRxiv - Cancer Biology 2022Quote: ... 10-50 μg of cell protein samples were resolved on Novex 4-20% Tris-Glycine Gels (Invitrogen) and transferred onto polyvinylidene fluoride membranes (Invitrogen) ...
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bioRxiv - Biophysics 2024Quote: ... precleared and immunoprecipitated overnight at 4 °C with 50 μL of GFP-conjugated Dynabeads Protein A (Invitrogen). Beads had previously been conjugated with 5 μL anti-GFP antibody (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... and bound protein eluted in 50 µL Pierce Gentle Ab/Ag Elution Buffer (Thermo Fisher Scientific #21027). Samples were analyzed by western blot probed with rabbit monoclonal antibody against human cGAS (Cell Signaling ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged ARL15 proteins were immunoprecipitated using 50 μL of Pierce Anti-DYKDDDDK Magnetic Agarose (ThermoFisher Scientific) for 2 hours at 4 ºC ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 2 hour incubation at 4℃ with 50 µL of magnetic Dynabeads Protein G (Invitrogen, #10004D). Beads were washed with 1x TBS Tween-20 washing buffer (Thermo Scientific ...