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Citations for Thermo Fisher :
8651 - 8700 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The concentration of extracted DNA was determined fluorometrically using Qubit® DNA HS (High Sensitivity) Assay Kits (Thermo Scientific™, Waltham, MA, USA) on a Qubit® 4 fluorometer (Thermo Scientific™ ...
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bioRxiv - Microbiology 2023Quote: ... DNA was eluted into 10mM Tris and quantified using the Qubit™ 1X dsDNA High Sensitivity (HS) Assay Kit (Thermo Fisher Scientific, UK) using the Qubit™ fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentration of the membrane fractions and whole cell lysates were measured using the Pierce BCA protein assay kit (Thermo Fisher Scientific, 23227). Myc-tag immunoprecipitation (IP ...
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bioRxiv - Microbiology 2024Quote: ... Quantification of isolated DNA was performed with the Qubit 4 fluorometer and the dsDNA HS Assay kit (Thermo Fisher Scientific, Waltham, MA, USA). DNA was sent to SeqCenter (Pittsburgh ...
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bioRxiv - Neuroscience 2024Quote: ... RNA samples were thawed on ice and concentrations were quantified using the Quant-iT™ RiboGreen™ RNA Assay Kit (ThermoFisher, Waltham, MA) and normalized to 10 ng/µL ...
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bioRxiv - Neuroscience 2024Quote: Total protein levels of all CSF samples were determined by use of a PierceTM BCA protein assay kit (Thermo Fisher Scientific, Waltham, USA). CSF Aβ40/Aβ42 and total tau (t-tau)/tau phosphorylated at threonine-181 (p-tau ...
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bioRxiv - Immunology 2024Quote: ... The final spike protein concentration was determined by measuring absorbance at 562 nm using Pierce™ BCA Protein Assay Kit (Thermo Scientific™). Proteins were dispensed into 0.5 ml aliquots and stored at -80°C.
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bioRxiv - Cell Biology 2024Quote: Hour 16 post-transfection control and SLC25A48-KO were incubated with 10 µM EdU (Click-iT Plus EdU Flow Cytometry Assay Kit, Thermo Fisher Scientific, USA) for 1 h in DMEM/F12 media ...
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Reduction of chromosomal instability and inflammation is a common aspect of adaptation to aneuploidybioRxiv - Cell Biology 2023Quote: ... The number of double-stranded DNA in the genomic DNA samples was quantified by using the Qubit dsDNA HS Assay Kit (Invitrogen, cat no Q32851). Up to 2000 ng of double-stranded genomic DNA was fragmented by Covaris shearing to obtain fragment sizes of 160-180 bp ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were clarified by centrifugation for 15 min (21.000 x g and 4 °C) and protein content in the supernatant was determined using a Pierce™ BCA assay kit (Thermo Scientific, USA). Cell lysates were diluted to a final concentration of 2 mg protein/ ml in native lysis buffer and kept on ice until used freshly.
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bioRxiv - Genetics 2024Quote: ... 1:10 dilution of whole cell lysates in RIPA were used to quantify protein content using Pierce BCA Assay Kit (Thermo Fisher Scientific #23227). 20 μg protein was loaded per sample into 15-well Novex WedgeWell Tris-Glycine Mini gels (Invitrogen #XP08165BOX) ...
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bioRxiv - Genetics 2024Quote: ... 1:10 dilution of lysates in LP2 buffer were used to quantify protein content using Pierce BCA Assay Kit (Thermo Fisher Scientific #23227). The resulting 2′3′-cGAMP levels were normalized to protein content in each sample.
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bioRxiv - Microbiology 2024Quote: ... The quality and concentration of extracted DNA were determined using a Qubit 4 fluorometer with dsDNA broad range (BR) Assay kit (Thermo Fisher Scientific, Canada). DNA size distribution was confirmed by running a Pippin pulse electrophoresis (Sage science ...
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bioRxiv - Microbiology 2024Quote: ... xGen-prepared and SSLR-prepared libraries were measured by Qubit® 1X dsDNA HS Assay Kit on Qubit Fluorometer (Life Technologies, CA, USA) and fragment length distributions were determined using TapeStation 4200 (Agilent ...
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bioRxiv - Microbiology 2024Quote: ... DNA was eluted in 80 μl elution buffer and the concentration was measured using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, USA).
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the supernatant from each sample was then determined using the Pierce BCA protein assay kit (Thermo Scientific, Catalogue #23225) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We quantified the concentrations of double-stranded DNA using a Qubit® Fluorometer and the dsDNA BR (broad-range) Assay Kit (Thermo Fisher Scientific). We adjusted the DNA extracts to a final concentration of 50 ng/µl with nuclease-free water (Carl-Roth GmbH + Co ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed in Ripa Buffer and total protein was quantified using the Pierce™ BCA Protein Assay Kit (Thermo Scientific™, 23227) for data normalization.
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bioRxiv - Molecular Biology 2022Quote: ... two independent IPs were carried out using 120 μg of Arabidopsis chromatin mixed with 3 % of Drosophila chromatin quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). DNA samples eluted and purified from the two technical replicates were pooled before library preparation (Illumina TruSeq ChIP ...
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bioRxiv - Molecular Biology 2022Quote: ... Then cells were fixed and clicked with Alexa Fluor 488 dye according to manufacturer’s instruction of Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (ThermoFisher Scientific, C10428). Samples were imaged on a Leica SP8 laser scanning confocal microscope at University of Chicago ...
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bioRxiv - Microbiology 2022Quote: ... Estimation of protein purity and quantity were achieved with SDS-PAGE and a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Rockford, IL), respectively.
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bioRxiv - Neuroscience 2023Quote: ... Sample concentrations were quantified based on the standard curve and normalized to total protein obtained through BCA protein assay kit (Thermo Fisher Scientific, 23225) We performed 5-6 independent experiments with 2-3 technical replicates per experiment that were averaged within each experiment.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... for final quality assurance the CFFT Lab subjected all its plasmids from the daughter plate to next generation sequencing (NGS): DNA from plasmid constructs was quantitated using Qubit dsDNA HS Assay Kit (Q33230, Thermo Fisher Scientific, USA) as per manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... Total protein concentrations of 24-hour GD18 urine was determined using a bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, Waltham, MA) per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Residual dsDNA in dPPM and native digests was quantified using the Quant-iT™ PicoGreen™ dsDNA assay kit (Thermo Fisher Scientific, USA) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: The cellular content of the various ECM digests was determined using a Quant-iTTM PicoGreen dsDNA assay kit (Invitrogen, Fisher Scientific, Paisley, UK). Following the manufacturers’ protocol ...
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bioRxiv - Bioengineering 2023Quote: The cellular content of the various ECM digests was determined using a Quant-iTTM PicoGreen dsDNA assay kit (Invitrogen, Fisher Scientific, Paisley, UK). Following the manufacturers’ protocol ...
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bioRxiv - Neuroscience 2023Quote: ... An aliquot of each batch of ACM was thawed and used to measure the protein content using the Pierce BCA protein assay kit (Thermo Fisher, Cat# 23225).
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bioRxiv - Molecular Biology 2023Quote: ... The concentration of isolated RNA was measured by using Qubit™ microRNA Assay Kit in a Qubit 4 Fluorometer (Thermo Fisher, Waltham, MA).
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bioRxiv - Immunology 2023Quote: ... the lysates were centrifuged for 15 min at 16,000 g and the supernatant containing the proteins were subjected for protein determination using a commercial Bicinchoninic acid assay (BCA) kit (Thermo Fisher, Karlsruhe, Germany). 50 g of proteins were heat desaturated in SDS-sample buffer (supp ...
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bioRxiv - Microbiology 2023Quote: ... Concentration of the libraries was determined using the Qubit® dsDNA HS Assay Kit as recommended by the manufacturer (Life Technologies GmbH, Germany). For sequencing the MiSeq instrument (Illumina ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were stained with monoclonal antibodies followed by EdU and BrdU staining procedures according to the manufacturer’s instructions using the Click-iT™ Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific), BD Cytofix/Cytoperm™ (BD Biosciences) ...
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bioRxiv - Physiology 2023Quote: ... LDH was measured on aliquots of the +NaCl-treated cellular supernatant using the CyQUANT™ LDH Cytotoxicity Assay Kit (Thermo Fisher; Cat. #C20300). LDH activity was measured by measuring absorbance at 490 nm ...
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bioRxiv - Plant Biology 2023Quote: ... quantified internally and externally by University of Minnesota Genomics Center (UMGC) with the Quant-iT RiboGreen RNA Assay Kit (Thermo Fisher, Cat # R11490), and quality checked with the Agilent 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2023Quote: ... the quantity of DNA was evaluated using a Qubit fluorometer and Qubit DNA BR Assay Kit in a Qubit® 3.0 Fluorometer (Thermo Fisher Scientific, USA). The final DNA concentration was standardized directly prior to library preparation.
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bioRxiv - Synthetic Biology 2023Quote: ... and the pull-down assay follows the protocol provided by Pierce™ His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, Waltham, MA). The fluorescence intensity (excitation = 485 ...
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bioRxiv - Plant Biology 2023Quote: ... Final amplification products were purified with the Omega BioTek E-Z 96 Cycle Pure kit and quantified using a Qubit 2.0 fluorometer with the Qubit dsDNA HS assay (Thermo Scientific, Grand Island, NY). DNA was pooled at equal concentration and sent for Illumina MiSeq sequencing at the University of Wisconsin Biotechnology Center (Madison ...
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bioRxiv - Microbiology 2023Quote: ... The final purified DNA was stored at -80°C and the DNA concentration was determined using Qubit HS Assay Kit (Invitrogen, Carlsbad, California, USA) on the Qubit 4 Fluorometric Quantification device (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... low glucose DMEM with heat-killed FITC-labeled Escherichia coli at 250 µg/ml (strain K-12; reagents from Vybrant phagocytosis assay kit; ThermoFisher Scientific; Cat # E2861) (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... library concentration and quality were assessed by fluorometry using a Qubit™ 4 and Qubit™ 1X dsDNA HS Assay Kit (ThermoFisher Scientific) and by electrophoresis using a TapeStation (Agilent ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting PCR products were gel purified and quantified with Nanodrop and Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, cat. no. Q32851). Pooled guide libraries were sequenced on Illumina NextSeq ...
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bioRxiv - Immunology 2023Quote: ... The protein concentrations of the obtained OMVs and OMPs were quantified using a bicinchoninic acid (BCA) assay kit (Thermo Fisher, Rockford, IL, USA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... DNA concentration was assessed using the Qubit® dsDNA HS assay kit on a Qubit® 3.0 fluorometer (Life Technologies, Eugene, Oregon, USA). To rule out contamination with non-14SM members ...
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bioRxiv - Immunology 2023Quote: ... The total isolated protein from splenic CD4+CD44+ cells was quantified using Bicinchoninic acid (BCA) Protein Assay Kit (Pierce, Thermo Scientific, USA, #23225).
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bioRxiv - Microbiology 2023Quote: ... Final libraries were assessed for size and quantity using an Agilent DNA1000 chip and Qubit dsDNA HS Assay Kit (Invitrogen, Carlsbad, California, USA), respectively ...
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bioRxiv - Immunology 2023Quote: The concentration of total protein in each nasal swab was determined using the Pierce™ BCA Protein Assay Kit (ThermoFisher, Cat No. 23225) according to the manufacturer’s microplate procedure ...
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bioRxiv - Microbiology 2023Quote: ... DNase-treated RNA was quantified with a Qubit 4 Fluorometer and the Qubit RNA Broad Range Assay Kit per manufacturer’s instructions (ThermoFisher Scientific, Cat. No. Q10211). 1 µg of RNA was then converted to cDNA per manufacturer’s instructions and diluted to a working stock of 5 ng/µL (SuperScript IV VILO Master Mix ...
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bioRxiv - Neuroscience 2023Quote: ... HDL and glucose (from serum sample) were analyzed in duplicate with a commercial assay kit (including quality controls and calibrators) using the KONELAB 20XTi (ThermoFisher, Massachusetts, United States). Deoayribonucleic acid (DNA ...
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bioRxiv - Biochemistry 2023Quote: ... The concentrations of recombinant proteins were determined by Pierce™ Rapid Gold BCA Protein Assay Kit (Cat#A53225, Thermo Fisher Scientific, Rockford, USA).
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bioRxiv - Bioengineering 2023Quote: ... by washing in phosphate buffered saline (PBS) and storing films at −80 °C for DNA quantification using Quant-iT™ PicoGreen™ dsDNA Assay Kit (ThermoFisher Scientific), per manufacturer’s instructions ...