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Citations for Thermo Fisher :
8301 - 8350 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Protein concentrations in the supernatant were determined using the Pierce Micro BCA Protein Assay kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... A 10 μl supernatant sample was mixed with 90 μl substrate solution containing 0.5 mg/ml of 4-methylumbelliferyl N,N’-diacetyl-β-D -chitobioside in an assay buffer provided as part of the kit and in a 96-well plate (black/clear bottom plate, Thermo Fisher). The reaction was incubated at 37 °C for 30 min and then stopped by adding 100 μl stop solution ...
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bioRxiv - Microbiology 2023Quote: ... Final libraries were quantified using a Qubit 3.0 Fluorometer and associated Qubit 1X dsDNA HS Assay Kit (Thermo Fisher Scientific, USA). Library quality assessment using an Agilent TapeStation system and paired-end sequencing of the Hi-C libraries were performed at the Quadram Institute Bioscience ...
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bioRxiv - Neuroscience 2023Quote: ... Protein levels of the brain tissue were measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Microbiology 2023Quote: ... according to manufacturer’s instructions and quantified using the fluorescent dye-based Qubit® dsDNA HS Assay Kit (Invitrogen, Carlsbad, California, USA). Samples were equimolarly pooled and sequenced using the Illumina MiSeq platform (Illumina Inc. ...
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bioRxiv - Microbiology 2023Quote: ... followed by HRP-labeled anti-mouse IgG (1:50000 dilution) and quantified with Pierce™ BCA Protein Assay Kit (Life Technologies). GST-PfPP1c and PfPP1c were produced as previously described (Hollin et al. ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... RNA libraries were assessed for quality and quantity with the Agilent 2100 BioAnalyzer and the Quant-iT Pico- Green dsDNA Assay Kit (Life Technologies). Strand-specific RNA libraries were sequenced as 150 bp paired-end runs on an Illumina HiSeq4000 platform ...
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bioRxiv - Microbiology 2022Quote: ... and length using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Q32854), a DeNovix DS-11 FX spectrophotometer ...
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bioRxiv - Microbiology 2022Quote: ... The purity of the DNA samples was assessed on a NanoDrop spectrophotometer (A260/A280 and A260/A230 ratios) and the DNA concentration was measured with a Qubit dsDNA HS reagent Assay Kit (Life Technologies). Single-molecule Real-time long reads sequencing was performed at the Gentyane core facility (Clermont-Ferrand ...
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bioRxiv - Microbiology 2022Quote: ... The concentrations of subpools were quantified using a Qubit dsDNA BR Assay Kit on a Qubit fluorometer (Thermo Fisher Scientific GmbH). The subpools were combined in one equimolar final pool for each library ...
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bioRxiv - Molecular Biology 2022Quote: ... The DNA concentration for each time point was determined using the Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific Q32851) with a Qubit 3.0 Fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was isolated from cells and the spent media was analyzed for cytotoxicity using CyQUANT LDH Cytotoxicity Assay Kit(Catalog no. C20300, Invitrogen, USA). For oxidative stress ...
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bioRxiv - Neuroscience 2022Quote: ... qRT-PCR was performed using the TaqMan RNA-to-Ct 1-Step Kit and TaqMan gene expression assays (Thermo Fisher Scientific). PCR amplifications were performed in triplicate or quadruplicates (n = 4-5 animals per genotype ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed with PBS before lysing with 350 ul RLT for total protein quantification using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific Life Sciences ...
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bioRxiv - Microbiology 2022Quote: ... Final pellets were resuspended in HNE buffer and protein concentrations were measured using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific) according to the manual provided by the manufacturer.
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bioRxiv - Neuroscience 2022Quote: ... The protein concentration of the lysates was determined using the Pierce™ BCA Protein Assay Kit (Thermo Fisher, Cat. no. 23225). Western Blots ...
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bioRxiv - Microbiology 2022Quote: ... The concentration of gDNA was quantified using the Qubit dsDNA BR assay kit and measured with a Qubit 2.0 fluorometer (Thermo Fisher Scientific) prior to sequencing ...
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bioRxiv - Microbiology 2022Quote: ... DNA extractions were diluted 1:10 and DNA concentration was measured using a Qubit dsDNA HS Assay Kit (Invitrogen, Waltham, USA). Depending on the concentration ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The isolations were quantified using a Qubit® dsDNA HS assay kit and a Qubit® 2.0 Fluorometer (Thermo Fisher Scientific) according the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... LCWG-seq was performed using double stranded DNA (dsDNA) and quantified with the Qubit® dsDNA HS Assay Kit (Invitrogen, #Q32851). Library preparation was performed with 1 μg of DNA and KAPA HTP Library Preparation Kit (KAPA Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... 10 guts per samples were lysed in 2 X SDS-loading buffer and protein was quantified using Pierce Detergent compatible Bradford Assay kit (Thermo Scientific). The lysates were separated by SDS-PAGE and transferred to Nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... The EdU incorporation was determined with the Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay kit (C10424; Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... the proteins were isolated from the supernatant and measured using the Brachidonic-acid assay kit (BCA) (Thermo Fisher Scientific, Cat# 23227). Next ...
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bioRxiv - Bioengineering 2024Quote: ... Concentrations of eluted DNA other than genomic DNAs were determined by Qubit (Qubit™ ssDNA Assay Kit, ThermoFisher, Cat. No. Q10212) from 3 independent measurements.
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bioRxiv - Cancer Biology 2024Quote: The release of intracellular glutamate into the extracellular medium was quantified using the Amplex Red Glutamic Acid/Glutamate Oxidase Assay Kit (Invitrogen, A12221) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... Encapsulation of mRNA was quantitatively determined by a modified protocol using Quant-iT RiboGreen RNA assay kit (Thermo Fisher, MA, USA) and a multimode microplate reader ...
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bioRxiv - Molecular Biology 2024Quote: ... Following washes in RPE buffer RNA was eluted in RNase free water and analysed by electrophoresis on an agarose gel and quantified using the Qubit RNA High Sensitivity Assay Kit (ThermoFisher Q32852).
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bioRxiv - Cell Biology 2024Quote: ... and MkPs were purified by flow cytometry and EdU incorporation analyses were performed according to the manufacturer’s protocols (Click-iT® EdU Flow Cytometry Assay Kits, Life Technologies).
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bioRxiv - Molecular Biology 2024Quote: ... The protein concentration of the CSF or plasma SEC fractions 7-10 was then determined using Qubit Protein Assay kit (ThermoFisher Scientific) using 1 μL of sample ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... EdU was stained using Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (Thermofisher Scientific, Cat#C10633).
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bioRxiv - Neuroscience 2024Quote: ... samples were diluted 50 times and measured using the Amplex Red Glutamic Acid/Glutamate Oxidase Assay Kit (Thermo Fisher Scientific; A12221,) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Luciferase emissions from transfected Hek-Luc cells were measured using the Pierce® Firefly Luciferase Glow Assay Kit (Thermo Fisher Scientific). To initiate cell lysis ...
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bioRxiv - Immunology 2024Quote: ... Target cell killing was then assessed using the CyQUANT Cytotoxicity Lactate Dehydrogenase (LDH) Release (a measure of cell death) Assay kit (Thermofisher Scientific) as per manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... DNA concentration was determined using Equalbit 1 × dsDNA HS Assay Kit (Vazyme, Nanjing, China) and a Qubit 3.0 Fluorometer (Life Technologies, Singapore) before submission for whole-genome sequencing ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The quality of DNA was checked using 1.5% agarose gel electrophoresis and concentration was measured by Qubit 4 fluorometer with 1X dsDNA HS Assay Kit (Thermo Fisher Scientific). As the basis for ddRAD library protocol development ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and DNA was quantified using a Qubit fluorometric quantification with the ds DNA High Sensitivity assay kit (Thermo Fisher Scientific, USA).
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bioRxiv - Genetics 2024Quote: ... The average size of each sample was measured with TapeStation and the DNA concentration was measured with Qubit dsDNA BR Assay Kit (ThermoFisher, Q32850). Then ...
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bioRxiv - Evolutionary Biology 2024Quote: ... following the manufacturer’s protocol and DNA concentration was measured with the Qubit DNA Assay Kit (Thermo Fisher Scientific, Waltham, Massachusetts USA). Libraries of 350 bp insert size were prepared and whole-genome resequencing was conducted by Novogene (Hong Kong) ...
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bioRxiv - Genomics 2024Quote: ... and subsequently quantified with the Qubit 3.0 fluorometer (Qubit RNA HS and dsDNA HS assay kits; Thermo Fisher Scientific, Waltham, USA), to standardise RNA for Illumina library preparation ...
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bioRxiv - Genomics 2024Quote: The concentrations of metagenomic or target enrichened libraries were measured using Qubit 2.0 Fluorometer in combination with Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific), then were analyzed on Agilent 4150 TapeStation in combination with Agilent D1000 ScreenTape System (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2024Quote: ... A small aliquot of the clear supernatant was taken for RNA quantification using the Qubit RNA HS Assay Kit (Invitrogen, Q32852). The RNA concentration was adjusted to 0.05 µg/µl with additional lysis buffer containing CHAPS ...
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bioRxiv - Genomics 2024Quote: ... Purity was accessed in Nanodrop One and concentration (ng/μl) was measured by Qubit fluorometer with Qubit dsDNA HS Assay kit (Invitrogen, USA). We used a modified version of the protocol described by [104] for the construction of ddRADseq libraries ...
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bioRxiv - Genomics 2024Quote: ... The DNA was quantified using a Qubit® 2.0 Fluorometer (Q32866) with the Qubit® DNA HS Assay Kit (Thermofisher, Q10211) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... The RNA was quantified using a Qubit® 2.0 Fluorometer (Q32866) with the Qubit® RNA BR Assay Kit (Thermofisher, Q10211) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... The RNA was quantified using a Qubit® 2.0 Fluorometer (Q32866) with the Qubit® RNA BR Assay Kit (Thermofisher, Q10211) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Samples were kept on ice and respirometry measurements were performed immediately after removing 100 μl for measuring the protein concentration using the BCA assay kit and manufacturer’s protocol (Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... The isolated DNA was quantified with a Qubit dsDNA HS Assay Kit on Qubit 3.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA) according the manufacturer’s instructions and then stored at -20 °C.
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration of each lysate was determined by the Pierce BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA). Cell lysates (20 μg ...
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bioRxiv - Cancer Biology 2024Quote: ... Glutathione determinations were normalized to the protein content of the insoluble pellet from the SSA extracts 2.5% SDS in 0.1 N bicarbonate using the BCA Protein Assay Kit (Thermo Scientific, Rockford, IL).
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bioRxiv - Biochemistry 2023Quote: ... Lysates were centrifuged at 20,000 x g for 10 min at 4 °C and protein concentrations were determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific) according to manufacturer’s instructions ...