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Citations for Thermo Fisher :
8101 - 8150 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Samples were homogenised using a VDI 12 tissue homogeniser and protein was quantified using a bicinchoninic acid (BCA) Protein Assay Kit (Thermo Scientific). 100 μg of total lung protein were lysed in 2x sample buffer (4% [w/v] SDS ...
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bioRxiv - Microbiology 2020Quote: ... Cytotoxicity was measured by quantifying lactate dehydrogenase (LDH) from ARPE-19 (Pierce LDH Cytotoxicity Assay Kit, ThermoFisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions [12,20].
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bioRxiv - Microbiology 2020Quote: ... and GAPDH was detected as an endogenous control using the Pre-Developed TaqMan Assay Reagent kit for mouse GAPDH (Applied Biosystems) as described previously (21) ...
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bioRxiv - Systems Biology 2021Quote: ... Lysates were cleared by centrifugation at 16,000 x g for 10 minutes at 4°C and protein concentration was determined using the BCA protein assay kit (Thermo Scientific). Fifty micrograms of protein was reduced with 5 mM Tris 2-carboxyethylphosphine (TCEP ...
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bioRxiv - Microbiology 2021Quote: ... pH 7.4) and quantified using the Qubit® DNA Assay Kit on a Qubit® 3.0 Fluorometer (Life Technologies, CA, USA). Alternatively ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Extracted DNA was suspended in Milli-Q water and concentration was determined with the Quant-iT dsDNA HS assay kit using a Qubit® 2.0 Fluorometer (Life Technologies). DNA integrity was assessed by electrophoresis ...
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bioRxiv - Genomics 2020Quote: ... We performed the DNA quality and quantity assessment using a Qubit double-stranded DNA HS Assay Kit (Invitrogen, Thermo Fisher Scientific) and an Agilent Bioanalyzer 2100 (Agilent Technologies) ...
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bioRxiv - Genomics 2020Quote: ... We performed the DNA quality and quantity assessment using a Qubit double-stranded DNA HS Assay Kit (Invitrogen, Thermo Fisher Scientific) and an Agilent Bioanalyzer 2100 (Agilent Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... The concentration and integrity of total RNA were checked using the Qubit RNA Assay Kit in Qubit 4.0 Fluorometer (Life Technologies, USA) and the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 System (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: The plasmid DNA yields from the sewage samples were evaluated using gel electrophoresis and Qubit double-stranded DNA (dsDNA) BR assay kit on a Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA). Plasmid DNA purity was measured and validated by absorbance ratio of 260/280 and 260/230 using NanoDrop 100 (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... Extracted DNA was visualized on a 1% agarose gel using Gel Red nucleic acid stain and quantified using Qubit dsDNA HS Assay Kit (ThermoFisher Scientific) and the NanoDrop 2000 (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... A portion (5 µl) was kept to quantify the total amount of proteins using Pierce™ BCA protein assay kit (23225, ThermoFisher). 5 ml of HNO3 5% (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Enriched virus was inactivated with 2% sodium dodecyl sulfate (SDS) and protein concentration determined with a Pierce BCA protein assay kit (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and 30 μL of supernatant was removed from the top of each well for measurement of LDH release using a Pierce LDH Cytotoxicity Assay Kit (Thermo Scientific) as per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Protein concentration of the extracts was estimated based on tissue weight or normalized using Pierce Coomassie Protein Assay Kit (ThermoFisher Scientific). Co-immunoprecipitation (coIP ...
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bioRxiv - Microbiology 2021Quote: ... The amount of total sterol in the EV samples was measured by the Amplex™ Red Cholesterol Assay Kit (ThermoFisher, A12216) and adjusted for the subsequent experiments.
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bioRxiv - Neuroscience 2020Quote: ... Total protein concentration was determined using the Bio-Rad Bradford protein assay kit according to the manufacturer’s instructions (ThermoFisher Scientific, #23225). An adapted BODIPY (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... gel purified and quantified using the Invitrogen™ Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA). For all 2018 samples sequencing was performed using an Illumina MiSeq and the MiSeq Reagent Kit v2 (Illumina ...
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bioRxiv - Neuroscience 2021Quote: ... frozen EVs were resuspended in RLT lysis buffer and RNAse-free water in a ratio of 1:3 and loaded based on protein concentration measured by Micro BCA Protein Assay Kit (Thermo Scientific). 2,8 μg of proteins were loaded.
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bioRxiv - Cancer Biology 2020Quote: ... for 2 hours and fluorescent labeling was performed with the Click-IT EdU Alexa Fluor 488 Flow Cytometry Assay kit (Thermo Fisher), according to the manufacturer’s description ...
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bioRxiv - Cancer Biology 2020Quote: ... 1% Triton X-100 with 100× protease inhibitors cocktail solution from GenDEPOT [P3100-005] and the phosphatase inhibitors NaF [10 mM] and Na3VO4 [20 mM]) and quantified using a bicinchoninic acid (BCA) protein assay kit (#23225, Thermo Scientific). The samples were added with 6X loading dye and boiled at 97 °C for 5 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were cleared by centrifugation and protein concentrations were determined using the Pierce 660 nm Protein Assay Kit (Thermo Fisher Scientific). Equal amounts of protein were diluted in Laemmli sample buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were clarified by centrifugation (20000 g, 10 minutes, 4 °C) and protein concentrations were determined using the BCA protein assay kit (ThermoFisher Scientific). The same amount of protein (1.2 μg ...
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bioRxiv - Cell Biology 2021Quote: ... The concentration of protein per sample was determined using the Bicinchoninic Acid (BCA) Protein Assay Kit (23228, Pierce/Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were pelleted for 15 min at 13 000 xg at 4 °C and supernatants kept for protein quantification (BCA Protein Assay Kit, Thermo Scientific). Equal amounts of cellular proteins were resolved on 10% or 12% sodium dodecyl sulfate – polyacrylamide gels (SDS-PAGE ...
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bioRxiv - Cell Biology 2021Quote: ... POS preparations were washed in PBS and resuspended in medium and protein content was measured (BCA Protein Assay Kit, Thermo Scientific) and in parallel ...
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bioRxiv - Cell Biology 2021Quote: ... The final pellet was resuspended in 200 μL of extraction buffer and the mitochondrial protein concentration was determined with the Pierce BCA Protein Assay Kit (Thermo Scientific). For the Proteinase K accessibility assay ...
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bioRxiv - Neuroscience 2022Quote: ... and human brain tissues were prepared using a RIPA buffer and the protein contents were measured using a PierceTM BCA Protein Assay Kit (Thermo Scientific). Proteins were separated on 12% SDS-PAGE gradient gel and transferred onto a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein synthesis was measured using Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit (#C10456; Life Technologies) per manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: Protein concentration of control and stimulated myotube whole cell lysates was determined using Pierce™ BCA protein assay kit (ThermoFisher Scientific) following the manufacturer’s instructions as described before [37] ...
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bioRxiv - Biochemistry 2022Quote: Protein concentration after cell lysis and after protein precipitation was determined using the Micro BCA™ Protein assay kit (Thermo Scientific) according to manufacturer’s guidelines.
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bioRxiv - Biochemistry 2022Quote: ... Lysates were centrifuged and the supernatants were transferred to new tubes for protein quantification using the Qubit Protein Assay Kit® fluorometric (Invitrogen), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... The DNA concentration for each sample was determined using the Qubit dsDNA BR assay kit (Thermo Fisher Scientific, Waltham, MA, USA) in a 96 well format using a Synergy H1 plate reader (BioTek Instruments ...
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bioRxiv - Neuroscience 2022Quote: ... the assay medium was removed from the wells and cells were flash-frozen for subsequent measurement of total protein from each well using Pierce BCA assay kit (23225, Thermo Fisher). Total protein content was used to normalize the data ...
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bioRxiv - Immunology 2022Quote: Homopropargylglycine (HPG) protein synthesis was measured using the Click-iT HPG Alexa Fluor 488 Protein Synthesis Assay Kit from Thermo Fisher Scientific ...
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bioRxiv - Pathology 2022Quote: ... Protein concentration was determined with Bio-Rad Protein Assay Kit II and 15 μg sample protein was loaded in a NuPAGE 3-8 % Tris-Acetate gel (Thermo Fisher). The gel was run at a constant voltage of 150 V for 4 h ...
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bioRxiv - Pathology 2022Quote: ... Cell lysates were centrifuged and the protein concentration of the supernatants were quantitated using BCA protein assay kit (Thermo Scientific, 23227). Cell extracts (800 μg ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... OCR was normalized to DNA content in each well measured by CyQUANT® Cell Proliferation Assay kit (Thermo Fisher Scientific, C7026) (28).
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bioRxiv - Molecular Biology 2022Quote: ... cleaned sub-libraries were quantified using the Qubit™ 3.0 fluorometer and a Qubit™ dsDNA HS Assay Kit (Invitrogen, UK) before being pooled at equimolar concentrations into a single final library ...
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bioRxiv - Microbiology 2022Quote: ... DNA concentration was determined using the Qubit dsDNA HS Assay kit and Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). Next ...
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bioRxiv - Neuroscience 2022Quote: ... the protein concentration in the supernatant was determined with a BCA Protein Assay kit (Pierce, Thermo Fisher Scientific, Rockford, IL, USA). To each protein sample (20 μg) ...
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bioRxiv - Microbiology 2022Quote: ... Lysate from the beads was clarified by centrifugation at 20,000 × g for 10 min and quantified using Pierce 660-nm protein assay kit (Thermo Fisher Scientific). Ten micrograms of protein was run per sample on 4–15% Mini-Protean TGX stain-free precast gels (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: ... One μL from each sample was used to quantify protein amounts in using BCA protein assay kit (Thermo Fisher Scientific, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... pre-extracted DNA was size-selected on the PippinHT platform (Sage Science) and then quantified using the Quant-it Picogreen assay kit (Thermo Fisher) on a Varioskan Flash Microplate Reader (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was eluted in RNAse-free water and quantified using Qubit™ RNA HS Assay Kit (Thermo Fisher, USA, #Q32852). RNA integrity was assessed by non-denaturing 1.2 % (w/v ...
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bioRxiv - Microbiology 2022Quote: ... DNA concentration was quantified using the Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Bacterial community composition was determined through amplicon sequencing of the V4 region of the 16S rRNA gene using Illumina MiSeq (2×250 paired-end ...
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bioRxiv - Cancer Biology 2022Quote: ... The quantification of prepared libraries was performed on a Qubit fluorometer using a Qubit dsDNA BR assay kit (Life Technologies, USA), while the size and purity of the libraries were examined on a Bioanalyzer DNA 1000 series II chip (Agilent Technologies) ...
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bioRxiv - Systems Biology 2022Quote: ... the protein concentration was determined using the Pierce BCA protein Assay kit in combination with a NanoDrop 2000 photospectrometer (Thermo Scientific).
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bioRxiv - Cancer Biology 2022Quote: Protein extracts were collected in 9 M urea lysis buffer and quantified using the Pierce Rapid Gold BCA Protein Assay Kit (Thermo Fisher). Protein samples were fractionated with SDS-PAGE using Bolt 4-12% Bis-Tris Plus Gels (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA content was quantified using a Qubit 3.0 Fluorometer and the dsDNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).