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Citations for Thermo Fisher :
8501 - 8550 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Quantification of the library concentrations was performed using Qubit™ 1x dsDNA High Sensitivity Assay Kits (Thermo Fisher, Waltham, MA, USA). The Agilent TapeStation High Sensitivity D1000 Screen Tape assay (Agilent Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... The DNA yield of each sample was then quantified by using a Qubit dsDNA HS Assay Kit (Invitrogen, Waltham, MA, USA).
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bioRxiv - Neuroscience 2024Quote: 5-ethynyl-2’-deoxyuridine (EdU)-incorporation assay was performed using Click-iT Plus EdU Alexa Fluor 488 Imaging Kit (Invitrogen, C10637). Animals were injected intraperitoneally with EdU reagent at a dose of 10 µl per 1 g animal (stock solution 20 mM solution in saline) ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatants were collected and their protein concentrations were determined using the PierceTM BCA Protein Assay Kit (Thermo Scientific, USA, Cat. #: 23225). Forty micrograms of protein per sample were separated on NuPAGE 4–12% ...
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bioRxiv - Microbiology 2024Quote: The concentration of the extracted DNA samples was measured using a Qubit dsDNA HS assay kit (Life Technologies, Carlsbad, CA, USA). The samples were then diluted to 2 µg/mL with TE buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Molar concentration of the finished libraries was estimated using a combination of Qubit dsDNA HS assay kit (Thermo Fisher, cat# Q32854) on Qubit 3.0 Fluorometer (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... 60, 66) or Ataxin-3 samples (Q-lengths 10, 80) were quantified using the Pierce BCA Protein Assay kit (Thermo Scientific) as per manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cumulative dried fecal samples were collected weekly for fecal protein measurement using the Pierce Detergent Compatible Bradford Protein Assay Kit (Thermo Scientific) as previously described (7) ...
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bioRxiv - Neuroscience 2024Quote: ... the concentration of the C-terminally truncated aSyn mutants and aSyn deleted mutants were determined using the PierceTM bicinchoninic acid (BCA) Protein Assay kit (Thermo Scientific). Fibril formation was induced by incubating the proteins at a final concentration of 20 µM at 37°C and pH 7.5 (unless stated otherwise ...
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bioRxiv - Neuroscience 2024Quote: ... and subjected to BCA analysis for total protein concentration determination using the Pierce BCA Protein Assay Kit (Thermo Scientific; Cat# 23225). Equal total protein lysates were diluted in NuPAGE Sample Reducing Agent (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... protein concentration in each sample was determined by using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Cat # 23225). 10-25µg protein per condition was transferred into new microcentrifuge tubes and 5 µL of the 200 mM TCEP was added to reduce the cysteine residues and the samples were then incubated at 55°C for 1-h ...
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bioRxiv - Plant Biology 2024Quote: ... The amount of RNA was quantified using the Quant-iT RNA Assay Kit broad range (Thermo Fisher Scientific, Waltham, MA, USA) and a Tecan Infinite 200 PRO plate reader (Tecan ...
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bioRxiv - Neuroscience 2024Quote: ... The clear supernatant was collected and the protein concentrations were determined using the Pierce BCA Protein Assay Kit (Thermo Scientific, #23227). Automated western blots were performed using Jess capillary-based immunoassay using 12-230 kDa separation with protein normalization (PN ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The amount of miR211-5p complexed with the UCLs was calculated using Quant-iT™ microRNA Assay Kit (Invitrogen™, US). A method previously described with some modifications was used [41 ...
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bioRxiv - Plant Biology 2024Quote: ... The final DNA library concentration was determined using a Qubit™ dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, Waltham, MA), according to the manufacturers protocol.
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were pelleted for 15 min at 13 000 xg at 4 °C and supernatants kept for protein quantification (BCA Protein Assay Kit, Thermo Scientific). Equal amounts of cellular proteins were resolved on 12% sodium dodecyl sulfate – polyacrylamide gels (SDS-PAGE ...
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bioRxiv - Physiology 2024Quote: ... the treated cells at harvest time were lysed by RIPA buffer supplemented with cOmplete and PhosSTOP and quantified by Pierce BCA Protein Assay Kit (23227, Thermo Fisher). Western blot analyses were carried out according to standard protocols with primary antibodies listed in Table S1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Digested protein was diluted 1:2 v/v with water and protein concentration was determined using a Pierce BCA assay kit (ThermoFisher Scientific)
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bioRxiv - Genomics 2024Quote: ... The mRNA was quantified using a Qubit 4 fluorometer and a Qubit RNA HS Assay Kit (Thermo Fisher Scientific, Cat# Q32852). The mRNA preparations were either immediately used to prepare a sequencing library or frozen and stored at −80 °C until further use.
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bioRxiv - Genomics 2024Quote: ... The DNA sample was quantified on Qubit 2.0 fluorometer using a qubit ds DNA broad-range assay kit (Invitrogen, United States). The DNA samples were purified using 0.45x magnetic beads (Beckman Coulter ...
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bioRxiv - Cancer Biology 2024Quote: ... EdU was fluorescently tagged with Alexa FluorTM 488 by click reaction (Click-it Plus EdU flow cytometry assay kit, Invitrogen, #C10632). DNA content was monitored by FxCycle Violet stain (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... 20 μL of cell supernatants were assayed following the instructions in the Pierce Cypridina Luciferase Flash Assay Kit (16169, ThermoFisher Scientific) and measured in the POLARstar Omega Plate Reader Spectrophotometer (BMG Labtech) ...
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bioRxiv - Developmental Biology 2024Quote: Measurement of protein synthesis of germ cells was performed by the Click-iT Plus OPP Alexa Fluor 594 protein synthesis assay kit (Molecular Probes) with slight modifications as described (Sanchez et al. ...
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bioRxiv - Biophysics 2024Quote: Viability was assessed at day in vitro (DIV) 3 and DIV7 using a LIVE/DEAD Viability/Cytotoxicity Assay Kit (Invitrogen, USA). Cells were incubated for 30 min at 37°C and 10% CO2 and rinsed with L15 medium without red phenol (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations of the cleared protein lysates were then determined with the Pierce Detergent Compatible Bradford Assay Kit (23246, Thermo Fisher) and equal amounts were incubated with beads ...
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bioRxiv - Cell Biology 2024Quote: ... where the supernatant was collected and protein concentration was measured using the Micro BCATM Protein Assay Kit (#23235, Thermo Fisher Scientific). Total proteins were then added to Laemmli sample buffer (#161-0747 ...
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bioRxiv - Cell Biology 2024Quote: ... The soluble supernatant fraction was collected and protein concentrations were measured using the Pierce Detergent Compatible Bradford Assay Kit (23246, Thermo Fisher). Samples were then adjusted for equal loading and mixed with 6x protein loading dye ...
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bioRxiv - Cancer Biology 2024Quote: ... Supernatants were transferred in a new tube and protein concentration was determined using Pierce™ BCA protein assay kit (ThermoFisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... The dsDNA was isolated and quantified using the Quant-iT™ PicoGreen™ dsDNA assay kit (P7589, Thermo Fisher Scientific, UK) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Aliquot (1/10) of this mixture was used for protein concentration measurement by Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific). Then 2x Laemmli buffer with β-mercaptoethanol was added and probes were boiled at 100°C for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Each experiment was normalized to total protein in each well using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Inc.).
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bioRxiv - Cancer Biology 2024Quote: ... Each experiment was normalized to total protein in each sample using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Inc.).
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bioRxiv - Cancer Biology 2024Quote: Percentages of cells proliferating were obtained using the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Invitrogen) and prepared according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... staining of EdU+ cells was performed with Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Thermo Fisher #10634) according to the the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Total protein concentration of the cell lysates was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher, Cat. No. 23227). Cell lysates were mixed with the sample buffer (with beta mercaptoethanol ...
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bioRxiv - Cell Biology 2024Quote: ... The quantification of total RNA was performed with a Qubit 4 fluorometer using the Qubit RNA BR Assay Kit (Invitrogen, Q10210), and RNA integrity was assessed by the Agilent 2100 Bioanalyzer with the Agilent RNA 6000 Nano Kit (Agilent ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were then transferred to polyvinylidene difluoride (PVDF) membranes (Immobilion) and the membranes probed with primary antibodies (anti-V5 epitope (Invitrogen), anti-HA epitope (Abcam) ...
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bioRxiv - Cancer Biology 2020Quote: ... The proteins were transferred onto a PVDF membrane and the membranes were probed overnight at 4 °C with the appropriate primary antibodies recognizing SLITRK6 (Invitrogen) or β-Actin (ACTB ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were subsequently transferred to a 0.45 μm PVDF membranes (Immobilon-P membrane) with the iBlot2 transfer system (Thermo Fisher), and blocked with 5% BSA in Tris-buffered saline and 0.1% Tween 20 (TBST) ...
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bioRxiv - Cell Biology 2023Quote: Yields of membrane vesicles were determined by the fluorescence signal emitted by the membrane lipid dye FM4-64 (Invitrogen, T13320). Stock solutions were prepared in water at a concentration of 50 µg/mL and kept at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: Membrane order was also assessed by fluorescence lifetime imaging by the polarity-sensitive membrane probe di-4-ANEPPDHQ (Invitrogen, D36802)23 ...
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bioRxiv - Cancer Biology 2021Quote: ... prior to transfer onto nitrocellulose membranes (Thermo Scientific). After blocking in 5% non-fat skim milk for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were probed in Ultrahyb Oligo buffer (Ambion) with 5’-32P-labeled (tRNASer ...
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bioRxiv - Molecular Biology 2020Quote: ... membrane was prehybridized with ULTRAhyb hybridization buffer (Invitrogen) for 30 minutes at 42°C ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked in 0.1% Hammarsten casein (Affymetrix) in 0.2X PBS with 0.01% sodium azide ...
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bioRxiv - Cancer Biology 2020Quote: ... Reactions were blotted onto Hybond® membranes (ThermoFisher) and UV cross-linked (Spectrolinker™ XL-1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were developed using SuperSignal WestFemto substrate (ThermoFisher).
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bioRxiv - Molecular Biology 2021Quote: ... transferred onto nylon membranes (Biodyne, Thermo Fisher Scientific), and then UV-crosslinked ...
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bioRxiv - Physiology 2020Quote: ... Proteins were blotted onto nitrocellulose membranes (Thermo scientific) and blocked with 25 ml of 5% (w/v ...
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bioRxiv - Genomics 2021Quote: ... and mitochondrial membrane potential (TMRM, Thermo Fisher I34361). MitoTracker and TMRM were incubated for 40 minutes at concentrations of 500nM and 1µM respectively ...