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Citations for Thermo Fisher :
8401 - 8450 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... newly synthesized DNA was labeled using the Click-iT Plus 5-ethynyl-2’-deoxyuridine (EdU) Alexa Fluor 647 flow cytometry assay kit (ThermoFisher Scientific) according to manufacturer ‘s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... EdU was detected using the Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (Thermo Fisher # C10632) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was transferred into a new tube and the protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific). Protein concentration was adjusted to 0.5 µg/µL using lysis buffer and 45 µL were stored at −80°C (“input”) ...
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bioRxiv - Genomics 2022Quote: ... The concentration of the extracted DNA was measured using the Qubit dsDNA BR assay kit (Thermo Fisher Scientific, Waltham, MA, USA), and DNA fragment length was evaluated by agarose gel electrophoresis with Pippin Pulse (Sage Science ...
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bioRxiv - Immunology 2022Quote: Fresh supernatants were clarified by centrifugation then assayed for LDH release using the Pierce LDH cytotoxicity colorimetric assay kit (Life Technologies) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... The DNA concentration of 1 μL of each sample was determined using the Qubit dsDNA HS Assay kit (Fisher Scientific, #33231) in black ...
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bioRxiv - Plant Biology 2022Quote: RNA quality was evaluated using a Qubit RNA IQ Assay Kit with a Qubit 4 Fluorometer (Thermo Fisher Scientific, Tokyo, Japan). The RNA samples for which RNA IQs were 9.1–10.0 were used for the following preparation ...
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bioRxiv - Physiology 2022Quote: RNA-seq results were validated using TaqMan Gene Expression Assays (see Supplementary Table 1A) and TaqMan One-Step RT-PCR Master Mix Reagents Kit (Life Technologies). The reaction was performed using 10 ng of total RNA as a template ...
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bioRxiv - Genomics 2022Quote: ... The quantity of the amplified cDNA barcoded samples was measured using the Qubit 1X HS dsDNA Assay kit (Thermo Fisher Scientific). Equimolar amounts were pooled to a total of 100 fmol ...
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bioRxiv - Genomics 2022Quote: ... The purity and concentration of the RNA samples were measured by the spectroscopic SimpliNano device (Biochrom US, Inc.) and a Qubit 4 fluorometer using an RNA HS assay kit (ThermoFisher Scientific), respectively ...
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bioRxiv - Microbiology 2022Quote: ... DNA concentrations were measured using Qubit™ dsDNA HS Assay kit and Qubit™ 3.0 Fluorometer (ThermoFisher Scientific; Invitrogen, MA, USA). All samples were stored at −30°C until required.
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bioRxiv - Microbiology 2022Quote: ... DNA concentrations were measured using Qubit™ dsDNA HS Assay kit and Qubit™ 3.0 Fluorometer (ThermoFisher Scientific; Invitrogen, MA, USA). All samples were stored at −30°C until required.
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bioRxiv - Neuroscience 2022Quote: ... tissue debris was removed by centrifugation and protein concentrations were determined by Pierce BCA Protein Assay Kit (ThermoFisher Scientific, catalog # 23225). 10 μg of proteins per sample were resolved by SDS-PAGE and transferred onto nitrocellulose membranes ...
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bioRxiv - Microbiology 2022Quote: ... The quality and quantity of DNA was assessed by performing gel electrophoresis and the Qubit dsDNA BR Assay Kit (Thermo Scientific), respectively ...
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bioRxiv - Microbiology 2022Quote: ... and the quantity of extracted genomic DNA was measured in a 384-well format using the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher). Sequencing libraries were generated in a 384-well format using a custom ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... GeneRuler 1 kb Plus DNA ladder and Quant-iT™ PicoGreen™ dsDNA Assay Kit were from Thermo Scientific (Waltham, MA). Size #3 hydroxypropyl methylcellulose Quali-V-I capsules were from Qualicaps (Whitsett ...
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bioRxiv - Plant Biology 2022Quote: ... The concentration of protein in the solution was measured by bicinchoninic acid (BCA) assay kit (Thermo Fisher Scientific, San Jose, USA).
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bioRxiv - Molecular Biology 2023Quote: ... and the specific Taqman™ MicroRNA Assay kit and the Universal PCR Master Mix No AmpErase UNG (both Thermo Fisher Scientific). The RT-PCR reaction were performed on ABI 7900HT Fast Real-Time PCR system ...
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bioRxiv - Microbiology 2022Quote: Cell lysates from the ΔhipBST cultures (vector induced, HipTLp uninduced, and HipTLp induced) were quantified for total protein (Pierce BCA Protein Assay Kit, Thermo Scientific), and 2.5 mg of total protein from each sample was used ...
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bioRxiv - Neuroscience 2023Quote: ... LDH assay was performed with 50 µl medium taken at 60 h post-transfection according to the manufacturer’s instructions (Pierce LDH Cytotoxicity Assay Kit, Thermo Fisher Scientific).
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Plastid double-strand RNA transgenes trigger small RNA-based gene silencing of nuclear-encoded genesbioRxiv - Plant Biology 2022Quote: ... All libraries were quantified on a DeNovix apparatus (Wilmington, USA) using the Qubit dsDNA Assay Kit (Thermo Fisher Scientific, Waltham, USA) and we multiplexed libraries in 10 nM pools ...
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bioRxiv - Physiology 2023Quote: ... Total protein within conditioned media was determined on the day of zymography experimentation and determined by using a bicinchoninic acid protein assay kit (ThermoFisher Scientific) according to manufacturer’s protocol and measured on a Safire II (Tecan ...
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bioRxiv - Physiology 2023Quote: ... An aliquot (10%) was removed for quantification using the Pierce Quantitative Fluorometric Peptide Assay kit (Thermo Fisher Scientific, Cat. No. 23290). The remainder of the peptides from each IP samples (∼0.5-3.5 µg ...
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bioRxiv - Physiology 2023Quote: ... Total liver cholesterol and triglycerides were determined with colorimetric assay kits (L-Type Triglyceride M – Fujifilm Wako, Infinity Cholesterol Liquid Stable Reagent – Thermo Fisher) and normalized to total protein content.
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bioRxiv - Physiology 2023Quote: ... Insulin concentrations were determined using a commercially available direct insulin enzyme-linked immunosorbent assay (ELISA) kit (#KAQ1251; Thermo Fisher Scientific, UK). Insulin sensitivity index (ISI ...
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bioRxiv - Genomics 2022Quote: ... DNA quantity was checked by fluorometry on the DS-11 FX instrument (DeNovix) with the Qubit dsDNA HS Assay Kit (Thermo Fisher) and sizes were examined on FEMTO Pulse (Agilent Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were washed 2× in PBS before EdU-Biotin Azide conjugation by click chemistry using the Click-iT reaction (Click-iT assay kit (Thermo Fisher, according to the manufacturer’s instructions) ...
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bioRxiv - Neuroscience 2023Quote: ... by measuring the lactate dehydrogenase (LDH) released from the cells into the media using a Pierce LDH Cytotoxicity Assay Kit (Thermo Scientific).
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was collected after centrifuging at 13,000 RPM (10 mins, 4°C) and total protein was estimated by BCA (Pierce BCA Protein Assay Kit, ThermoFisher Scientific, 23225). The supernatant was mixed with 1X Laemmli buffer and boiled at 95°C for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The quantitative real-time PCR (qPCR) assays were carried out on a StepOnePlus system (Applied Biosystem) using SYBR Green Master Kit (A25918, Thermo Fisher). Primers for qPCR were listed in Table S1 ...
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bioRxiv - Cancer Biology 2023Quote: ... The concentration of the Cetuximab antibody was calculated using a Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA). The loading ratios and conjugation efficiencies of the two dyes were subsequently calculated using the obtained concentrations of the dyes and protein (antibody) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysate protein content was normalized for each fraction using a BCA protein assay kit (Prometheus) and samples were reduced with 4x NuPAGE LDS Sample Buffer (Life Technologies) containing 10%β-Mercaptoethanol ...
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bioRxiv - Cancer Biology 2023Quote: ... and click reaction to fluorescently label incorporated EdU with AF488 following the instruction from the Click-iT Plus EdU Flow Cytometry Assay Kit (ThermoFisher; C10632).
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bioRxiv - Genomics 2022Quote: ... DNA concentration and molecule length were evaluated using the Qubit dsDNA BR Assay kit on the Qubit Fluorometer (Thermo Fisher Scientific) and the Genomic DNA Screen Tape on the Agilent 2200 TapeStation system (Agilent Technologies) ...
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bioRxiv - Genomics 2022Quote: ... and RNA quantity was confirmed using the Qubit™ RNA High Sensitivity Assay kit (Invitrogen™, Thermo Fisher Scientific, Waltham, MA) on the Qubit™ 4 Fluorometer (Invitrogen™ ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatants were collected and protein concentration was determined using a Pierce™ BCA Protein Assay Kit (Cat#: 23225, Thermo Fisher scientific) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... DNA quantity was determined using a Qubit dsDNA HS Assay kit in conjunction with a Qubit 2.0 Fluorometer as directed by the manufacturer (Life Technologies, USA).
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bioRxiv - Cell Biology 2023Quote: ... samples were collected by centrifugation (11,000 ×g, 20 min) and their protein content was determined (Thermo Pierce BCA Protein Assay kit, ThermoFisher, Cat# 23225). Proteins (∼30 µg/lane ...
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bioRxiv - Immunology 2023Quote: ... the Click reaction was performed using the Click-iT™ Plus OPP Alexa Fluor 647 Protein Synthesis Assay Kit (ThermoFisher, C10458) at RT for 30 mins ...
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bioRxiv - Developmental Biology 2023Quote: ... HUH7.5 cells were incubated at 37 °C for 2 hours with 10 µM EdU using the Click-iT EdU Assay Kit (Thermofisher, C10418) according to the manufacturer’s recommendations.
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bioRxiv - Developmental Biology 2023Quote: ... Protein lysate from each sample was collected and the concentration of the protein was estimated using Pierce™ BCA Protein Assay Kit (ThermoFisher). For each sample ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and all negatives were checked for DNA concentration on a Qubit 2.0 Fluorometer using the Qubit dsDNA HS assay kit (ThermoFisher Scientific, USA), and for fragment size on a Fragment Analyzer using the HS NGS Fragment kit (1–6000 bp ...
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bioRxiv - Plant Biology 2023Quote: ... EMSA assays were performed according to the manufacturer’s instructions for a LightShift Chemiluminescent EMSA Kit (Thermo Fisher Scientific, Waltham, MA, USA). The migration of the biotin-labeled probes was detected using ECL substrate (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... The concentration of the liberated soluble protein was assessed with the Pierce BCA protein assay kit (Thermo Fisher, Waltham, MA, USA), and equal amounts of protein (180-250 µg ...
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bioRxiv - Immunology 2023Quote: ... Protein concentration of the supernatant was determined by following manufacturers instruction for the Pierce BCA Protein Assay Kit (ThermoFisher-Scientific #23227). Once total protein concentration was determined ...
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bioRxiv - Biochemistry 2023Quote: Purity of all proteins was verified by SDS-PAGE and concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Biochemistry 2023Quote: Purity of all proteins was verified by SDS-PAGE and concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... using the Agilent Bioanalyzer to determine sample RNA integrity number (RIN) and quantified by the Qubit RNA HS assay kit (Life Technologies). All samples had RIN values > 9.7 ...
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bioRxiv - Microbiology 2023Quote: ... DNA was quantified using a Qubit 3 fluorometer in combination with dsDNA BR or HS assay kits (ThermoFisher Scientific, Frankfurt, Germany). DNA integrity was checked through spectrophotometry and DNA agarose gel electrophoresis ...
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bioRxiv - Plant Biology 2023Quote: ... The quality and quantity of the total RNA were checked using a Qubit RNA HS Assay Kit and Qubit 2.0 Fluorometer (Thermo Fisher Scientific) before sequencing ...