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Citations for Thermo Fisher :
8201 - 8250 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... of hiPSC-aCMs and -vCMs were measured by a competitive enzyme-linked immunosorbent assay (ELISA) using a commercially available kit (Invitrogen, CA). The assay was conducted according to the manufacturer’s protocol and was measured using a spectrophotometric plate reader.
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bioRxiv - Molecular Biology 2020Quote: ... The concentration of DNA in the mock community sample was measured on a Qubit 3.0 fluorometer and Qubit dsDNA HS assay kit (Thermo Fisher Scientific), and the quality of the DNA was measured by gel electrophoresis on an Agilent 2200 Tapestation using Genomic Screentapes (Agilent Technologies).
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bioRxiv - Molecular Biology 2021Quote: The poly(A) tail lengths of each mitochondrial transcripts were examined using Poly(A) Tail-Length Assay Kit (Thermo Fisher Scientific). Total RNA was extracted from isolated mitochondria and added a number of guanosine and inosine residues to the 3’-ends ...
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bioRxiv - Microbiology 2020Quote: ... The total protein concentration in each sample supernatant was quantified using the bicinchoninic acid (BCA) method (Pierce BCA Protein Assay Kit; Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... A DNA concentration of the purified PCR product was measured by the Quant-iT dsDNA HS assay kit and the Qubit fluorometer (Invitrogen, Inc.). The concentration of total double-stranded DNA in each sample was adjusted to 5 ng/μL ...
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bioRxiv - Microbiology 2021Quote: ... DNA concentrations were measured with the Qubit dsDNA High Sensitivity (HS) assay kit on a Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA) before storing the DNA at -20°C.
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bioRxiv - Cell Biology 2020Quote: The total cholesterol content of HCT8 cells treated with MakA was determined using the Amplex Red Cholesterol Assay kit (Molecular Probes) as previously described (Eskelinen et al. ...
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bioRxiv - Microbiology 2020Quote: ... and the RNA concentration was measured using Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA). Then RNA from corresponding sample was reverse transcribed into cDNA and the transcriptional levels of different genes were determined by qRT-PCR using SybrGreen Premix Low rox (MDbio ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentration was measured with the BCA Protein Assay Kit (Pierce Biotechnology) and equal amounts of protein were separated on 4–12% Tris-Glycine Gels (Thermo Scientific) and transferred onto Immobilon-P PVDF membranes (Merck Millipore) ...
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bioRxiv - Immunology 2021Quote: ... 3.6 mg of total protein extracted from tissue and cell-line material was used for subsequent immunopeptidome analysis as determined with Pierce BCA protein assay kit (Thermo Scientific).
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bioRxiv - Immunology 2020Quote: ... Lysates were cleared by centrifugation at 20,000 g for 15 min at 4°C then protein concentrations determined using Coomassie Protein Assay Kit (Thermo Scientific, UK). For each sample ...
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bioRxiv - Genetics 2020Quote: ... and the DNA quality was measured using a photometer (NanoPhotometer NP80; Implen) and quantified with the dsDNA assay kits (Qubit HS; Life Technologies). The gDNA concentrations were adjusted to 25 ng/μL ...
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bioRxiv - Genomics 2021Quote: ... The concentration of each sample was then measured using the Qubit dsDNA high sensitivity assay kit (Thermo Fisher Scientific, Cat #Q32854). Samples were then sequenced on an Illumina HiSeq 4000 using 10-30% PhiX (Illumina ...
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bioRxiv - Genomics 2021Quote: ... Samples were quantified in a 96-well plate reader using the Quant-IT DNA high sensitivity assay kit (Invitrogen, Cat #Q33232) according to the manufacturer’s protocol and pooled into 1 tube ...
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bioRxiv - Genetics 2020Quote: ... Amplicons obtained from the second PCR were purified by gel extraction and quantified using the Qubit dsDNA HS assay kit (Invitrogen #Q32851). Single-end 75 bp sequencing was performed on the NextSeq 500 platform by the Molecular Biology Core Facilities at Dana-Farber Cancer Institute ...
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bioRxiv - Cell Biology 2021Quote: ... The cell pellet was also snap frozen and stored for protein analysis via the manufacturer’s instructions for the Pierce BCA Protein assay kit (Thermo Fisher Scientific) to normalize the NMR data ...
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bioRxiv - Biochemistry 2020Quote: ... The microsomes were resuspended and diluted according to the protein concentration measured in the supernatants using the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Evolutionary Biology 2020Quote: ... We measured RNA concentration using the Qubit® RNA Assay Kit in a Qubit®2.0 Flurometer (Life Technologies, CA, USA). We assessed the RNA integrity using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies ...
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Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imagingbioRxiv - Molecular Biology 2021Quote: ... The cell viability was evaluated by fluorescence microscopy using the LIVE/DEAD® viability/cytotoxicity assay kit (Molecular Probes, Life Technologies) following the manufacturer’s protocol.
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Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imagingbioRxiv - Molecular Biology 2021Quote: ... The cell viability was evaluated by fluorescence microscopy using the LIVE/DEAD® viability/cytotoxicity assay kit (Molecular Probes, Life Technologies) following the manufacturer’s protocol.
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bioRxiv - Genetics 2021Quote: ... Then concentration of the protein was measured by the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, USA, Cat. #23227). Samples were stored at -80°C until proteomic preparation.
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bioRxiv - Neuroscience 2021Quote: 2×105 cells were lysed in 50 μl RIPA buffer and protein amount was assessed using BCA assay kit (Thermo Scientific). Between 3 and 10 μg of total protein were loaded on to a 4-15% gradient TGX gel (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Assay results were normalized to the total protein level in each ON sample as quantified with the Pierce BCA protein assay kit (ThermoFisher Scientific).
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bioRxiv - Plant Biology 2021Quote: ... RNA quality was examined on a 1% agarose gel and RNA concentration was quantified using the Qubit RNA HS assay kit (Invitrogen, USA). 2ug of total RNA was used to construct stranded RNA-seq libraries using the Illumina TruSeq stranded total RNA LT sample prep kit (RS-122-2401 and RS-122-2402) ...
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bioRxiv - Immunology 2021Quote: ... Soluble marker protein levels were normalized to total protein levels measured by Quant-iT protein Assay Kit (Thermo Fisher Scientific, USA).
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bioRxiv - Biochemistry 2020Quote: Cell death was determined by measurement of lactate dehydrogenase (LDH) leakage using the LDH Cytotoxicity assay kit (Invitrogen, Carlsbad, CA, USA) following manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was collected for the measurement of total protein through Pierce Coomassie Plus (Bradford) Assay Kit (Thermo Fisher, Illinois, USA). Seven µg of total protein were loaded on an SDS-PAGE gel (GenScript ...
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bioRxiv - Microbiology 2021Quote: ... samples were tagged with iTru primers (Glenn et al., 2019) and quantified with a Qubit 2.0 Fluorometer DNA high sensitivity assay kit (Thermofisher, Waltham, MA). Samples were then cleaned with a 1:1 ratio of speedbeads and pooled into groups of 8-12 for enrichment or set aside for sequencing ...
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bioRxiv - Developmental Biology 2021Quote: ... The Click-iT Plus OP-Puro Alexa Fluor 647 assay was done according to the manufacturer’s protocol (Click-iT Cell Reaction Buffer Kit, ThermoFisher Scientific, C10269). Cells were resuspended in 200 μL PBS supplemented with 3% FBS and 0.1% saponin and analyzed by flow cytometry ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA concentrations were determined by fluorometric quantitation using a Qubit 2.0 Fluorimeter with a Qubit DNA dsDNA HS Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were pulsed-labelled with 10 μM EdU (Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit, Thermo Fisher Scientific) in fresh culture medium for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA integrity was determined by agarose electrophoresis and concentration measured using Qubit RNA Assay Kit in a Qubit 2.0 Fluorometer (Life Technologies, Invitrogen). cDNA libraries were synthetized from 1 µg of total RNA and sequenced using a HiSeq-3000 platform (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: HRMEC viability and/or proliferation was assessed at 48 and 72 hours after transfection with si-SLC38A5 or si-Ctrl using a MTT cell metabolic activity assay kit (Cat# V13154, Life Technologies) as described previously (49) ...
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bioRxiv - Biophysics 2020Quote: ATPase activity of prohead/gp16 (wt or mutant) motor complexes was determined by measuring production of inorganic phosphate by the EnzChek Phosphate Assay Kit (Life Technologies) as described previously (43) ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA concentration was fluorometrically measured combining dsDNA Broad Range Assay Kit with Qubit® 4.0 (Thermo Fisher Scientific, Waltham, USA); the size of DNA fragments was evaluated using the CHEF Mapper electrophoresis system (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: Total protein of 40 μg was prepared from each sample and quantified by the Pierce™ BCA Protein Assay Kit (ThermoFisher). We ran the protein in sodium dodecyl sulfate-polyacrylamide (SDS ...
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bioRxiv - Genomics 2020Quote: ... the protein concentration of lysates was first determined using the Microplate BCA protein assay kit – reducing agent compatible (Thermo Fisher Scientific), according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2020Quote: ... The DNA was eluted in Tris-Cl (pH, 8.0) and quantified using the Qubit high sensitivity DNA assay kit (Invitrogen, MA, USA). DNA samples were kept at -20°C until Illumina sequencing library preparation ...
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bioRxiv - Microbiology 2020Quote: ... and cfDNA concentration (Qubit 4 fluorometer, Fisher Scientific, Pittsburgh, PA and Qubit™ dsDNA HS Assay Kit, Fisher Scientific, Pittsburg, PA).
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bioRxiv - Plant Biology 2021Quote: ... DNA integrity was assessed using agarose gel electrophoresis and quantified with a Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on a Bio-Tek Powerwave HT microplate reader ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The concentration of extracted individual DNA was measured using Quantitation Qubit™ 1X dsDNA HS Assay Kit (Invitrogen Life Technologies USA). Samples from each of the six populations (Table 4 ...
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bioRxiv - Neuroscience 2020Quote: ... Both Cstb and scrambled siRNA were labeled for the immunofluorescence assays using the Silencer Labeling Kit with FAM dye (Invitrogen, AM1634) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Tagged amplicon pools were quantified using the Qubit™ 3.0 fluorometer and a Qubit™ dsDNA HS Assay Kit (Invitrogen, UK) and pooled with equimolar concentrations into a unique library ...
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bioRxiv - Immunology 2020Quote: ... Secreted factors were measured in the T cell assay culture media using a Luminex kit (Cytokine Human Magnetic 30 Plex Panel, Thermo Scientific) per manufacturer’s recommendations with 4 replicates per sample ...
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bioRxiv - Immunology 2020Quote: ... EDU incorporation was determined using the Click-ITTM Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... All negative controls had undetectable DNA measurements using Qubit double-stranded DNA (dsDNA) BR assay kit on a Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA).
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bioRxiv - Immunology 2021Quote: ... The total protein concentration of each sample was determined using the Pierce(™) BCA Protein Assay Kit (Thermo Fisher Scientific Inc.) and the saliva sample was prepared with equal concentrations of proteins from each individual sample ...
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bioRxiv - Cell Biology 2020Quote: ... The effect of irradiation on tendon cells was confirmed by the live/dead cell viability assay kit (ThermoFisher Scientific, Waltham, MA) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2021Quote: ... The DNA concentration was determined for each sample using the Qubit dsDNA Broad Range Assay Kit (cat# Q32850, Invitrogen, Carlsbad, CA).
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bioRxiv - Genomics 2021Quote: ... The DNA concentration was determined for each sample using the Qubit dsDNA Broad Range Assay Kit (cat# Q32850, Invitrogen, Carlsbad, CA).