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Citations for Thermo Fisher :
8051 - 8100 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The concentration of DNA samples was measured with the Qubit dsDNA High Sensitivity (HS) assay kit on a Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA) before storing the DNA at −20°C.
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bioRxiv - Molecular Biology 2021Quote: ... according to manufacturer’s instructions and subsequently quantified by using a Qubit RNA BR Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). The copy number was calculated using the online NEBio Calculator (https://nebiocalculator.neb.com/#!/ssrnaamt).
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bioRxiv - Molecular Biology 2021Quote: ... reverse transcription (RT) was performed using the Taqman™ miRNA Reverse Transcription Kit and Taqman™ miRNA Assays (Thermo Fisher Scientific). The following Taqman™ miRNA Assays were used ...
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bioRxiv - Molecular Biology 2020Quote: ... The concentration in grams per volume of the synthesized reference RNA was determined by fluorometric quantification using the RNA HS assay kit (ThermoFisher; Q32852) and Qubit 2.0 (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Final proteins concentration in the NE extracts was determined using the colorimetric Pierce BCA protein assay kit (Thermo Scientific, Waltham, MA). Pellets were stored at −80° C.
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bioRxiv - Biophysics 2022Quote: ... 28,32 Concentration of TALI(23-I23)’s MOTH domain was determined using the Pierce™ BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Cell Biology 2022Quote: The quality and quantity of the amplified cDNAs were assessed using the dsDNA HS Assay Kit on a Qubit fluorometer (ThermoFisher Scientific) and Agilent High Sensitivity DNA kit on a 2100 Bioanalyzer system (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-ethynyl-2′-deoxyuridine (EdU) incorporation was detected using a Click-iT Plus EdU Alexa Fluor 555 Assay Kit (Invitrogen, C10638) prior to primary antibody incubation ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and DNA concentration was measured using a Qubit® 2.0 Fluorometer (following the Invitrogen™ Qubit™ dsDNA BR Assay Kit) (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... DNA concentration was measured with an Invitrogen Qubit 2.0 fluorometer using the dsDNA HS Assay Kit (Thermo Fisher Scientific, Waltham, USA) with final assessments for DNA quality ...
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bioRxiv - Immunology 2021Quote: ... PCR product was purified without fragmentation using SPRIselect beads and quantified using a Qubit 1X dsDNA HS Assay Kit (Thermo Fisher).
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bioRxiv - Bioengineering 2020Quote: ... the final working concentration of protein was found to be 100 μg/ml using a BCA Protein Assay kit (Thermo Scientific). Purity of the protein was confirmed by SDS-PAGE followed silver staining of the gel ...
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bioRxiv - Genetics 2020Quote: ... Final library profiles were assessed and quantified on a BioAnalyzer High Sensitivity DNA chip and using the Qubit dsDNA HS Assay kit (ThermoFisher #Q32851). Sequencing was performed at the Science for Life Laboratory ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell viability was evaluated by fluorescence microscopy using the LIVE/DEAD® viability/cytotoxicity assay kit (Molecular Probes, Life Technologies) following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2020Quote: ... The cell viability was evaluated by fluorescence microscopy using the LIVE/DEAD® viability/cytotoxicity assay kit (Molecular Probes, Life Technologies) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... huxleyi DNA integrity was examined using a modified protocol for the Click-iT TUNEL Alexa Fluor 488 Imaging Assay kit (Thermo Fisher). Cells were assayed using the manufacturer protocol and were sampled after 46 h HHQ exposure ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Lipid peroxidation levels were normalised against total protein quantitated using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, United States).
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bioRxiv - Cancer Biology 2020Quote: ... RNA libraries were assessed for quality and quantity with the Agilent 2100 BioAnalyzer and the Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies). RNA libraries were sequenced as 150 bp paired-end runs on an Illumina HiSeq4000 platform ...
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bioRxiv - Genetics 2020Quote: ... Protein quantification of the supernatant was performed using the Pierce™ BCA protein assay kit (Thermo-Fisher Scientific, Rockford, IL, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... DNA was extracted from tail snips and qPCR was performed with TaqMan Gene Expression Assay kit (Thermo Fisher Scientific, Waltham, MA) according to manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... The quantity and quality of the extracted RNA was assessed using a ThermoFisher Scientific Qubit 4.0 fluorometer with the Qubit RNA BR Assay Kit (Thermo Fisher Scientific) and an Advanced Analytical Fragment Analyzer System using a Fragment Analyzer RNA Kit (Agilent) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We checked DNA concentration using the Qubit® fluorometer and the Qubit® dsDNA BR Assay Kit (ThermoFisher Scientific, Waltham, USA). We diluted DNA solutions to 30 ng/μl in a target volume of 55 μl ...
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bioRxiv - Immunology 2020Quote: ... Organs were collected two hours later and the staining procedure was done according to manufacturers’ instructions using the Click-it Plus EdU Alexa Fluor 488 Flow Cytometry Assay kit (Molecular Probes). Quantification of cell cycle duration using an EdU/BrdU double pulse was performed by consecutive i.p ...
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bioRxiv - Evolutionary Biology 2021Quote: ... liver tissues were lysed in RIPA buffer and total protein concentrations were determined by MicroBCA protein assay kit (Thermo Scientific, 23235) according to the manufacturer’s instructions and infinite 200 PRO microplate reader (Tecan) ...
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bioRxiv - Cell Biology 2020Quote: ... The concentration of purified GAS6-MycHis (denoted as GAS6) was measured with Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... Cell lysates were cleared by centrifugation and protein-containing supernatants were collected into fresh tubes and quantified with the Pierce BCA protein assay kit (Thermo Scientific) using a two-fold dilution curve of BSA (Thermo Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... Eluates containing the enriched phosphopeptides were dried down and subsequently resuspended with 50 μl 0.1% formic acid for peptide concentration measurement using the Pierce Quantitative Colorimetric Assay kit (Thermo Scientific PN23275).
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bioRxiv - Plant Biology 2020Quote: ... Whole genomic DNAs were quantified using the Qubit dsDNA BR Assay Kit and Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, California, USA); high-molecular-weight DNA (>15 kb ...
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bioRxiv - Bioengineering 2020Quote: ... The efficiency of siRNA encapsulation N+:P− ratios was evaluated using a Quant-iT Ribogreen assay kit (ThermoFisher Scientific, Waltham, MA). siNP size was evaluated using dynamic light scattering (DLS ...
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bioRxiv - Neuroscience 2020Quote: ... 8 µg of the cDNA from each sample were fragmented and labelled with GeneChip Mapping 250K Nsp assay kit (Affymetrix, 900753). Hybridization was performed as described previously31 during 16 h at 45°C ...
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bioRxiv - Developmental Biology 2021Quote: ... The dried lipids were solubilized in 95% ethanol and the cholesterol was quantitated using the Amplex Red Cholesterol Assay kit (Invitrogen A12216). All values for the wild-type and mutant samples are shown in Table S9 ...
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bioRxiv - Developmental Biology 2021Quote: ... Lysates were cleared of debris by centrifugation at 14,000g for 15 min and quantified using the BCA protein assay kit (Thermo Fisher Scientific). Lysates were diluted 1:4 with 1X sample buffer (ProteinSimple) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell cycle analysis was performed using the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: The autophagic flux assay was carried out using the Premo™ Autophagy Tandem Sensor RFP-GFP-LC3 Kit (P36239, Molecular Probes, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... The cartilage explants were then rinsed and incubated in PBS containing 0.05% calcein acetoxymethyl and 0.2% ethidium homodimer-1 from the Live/Dead Cell Viability Assays kit (Life technologies, Cat.# L3224) for 30 min ...
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bioRxiv - Bioengineering 2021Quote: ... The surface concentration of the antibodies was quantified as previously described using a bicinchoninic acid assay (BCA) kit (Thermo Fisher 23227)36.
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bioRxiv - Bioengineering 2020Quote: ... luminescence was compared to a ladder of recombinant Gaussia Luciferase (NanoLight #321-500) quantified using a Gaussia Luciferase Glow Assay Kit (ThermoFisher #16161) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... to improve sensitivity and loading in ELISA or BLItz binding assays (EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit, ThermoFisher 21435).
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bioRxiv - Genomics 2021Quote: ... the protein concentration of each of the liquids in which the worms were incubated (“secretome” samples) was measured using a Pierce Coomassie (Bradford) Protein Assay Kit (Fisher Scientific). Bovine Serum Albumin (BSA ...
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bioRxiv - Neuroscience 2020Quote: ... Lysates were cleared by centrifugation at 20.000g for 10min and quantified with the Pierce™ BCA Protein Assay kit (Thermo Scientific). 25 μg of the lysates were then separated on NuPAGE 4-12% Bis-Tris gels (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... RNA libraries were assessed for quality and quantity with the Agilent 2100 BioAnalyzer and the Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies). RNA libraries were sequenced as 100 bp paired-end runs on an Illumina HiSeq2500 platform ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA was resuspended in water and quantified with Qubit dsDNA BR Assay kit on Qubit 2.0 Fluorometer (Thermo Fisher Scientific). DNA integrity was assessed with Genomic DNA ScreenTape on TapeStation system (Agilent ...
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bioRxiv - Cancer Biology 2021Quote: MiR-212-3p expression was quantified using TaqMan™ MicroRNA Reverse Transcription Kit and TaqMan™ Advanced miRNA Assay (Applied Biosystems). Isolated total RNA was reverse transcribed using a specific stem-loop primer for miR-212-3p and RNA U6B ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were centrifuged at 4 °C for 10 minutes at 16000 g to remove cell debris and protein concentration of the lysate was measured using Pierce BCA Protein Assay Kit (Thermo Scientific). Equal amounts (10 µg ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μl of the lipid extract was used to estimate the total sterol content using the Amplex™ Red Cholesterol Assay Kit (Invitrogen) per the manufacturer’s instructions.
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bioRxiv - Physiology 2021Quote: NIH 3T3 cell proliferation was quantified according to the CyQUANT® Cell Proliferation Assay Kit (Molecular Probes, Inc. Eugene OR, USA) at four cell densities 1600 – 6700 cells/well ...
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bioRxiv - Cell Biology 2021Quote: ... the tissue was homogenized with RIPA cell lysis buffer (Milipore) and then lysate was quantified with Pierce BCA protein assay kit (Thermo Scientific).
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bioRxiv - Cell Biology 2021Quote: ... Total protein concentrations of the supernatants were determined using a Pierce BCA protein assay kit (Thermo Scientific, catalog # 23225, lot # SJ256254) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... and the relative cell numbers in the individual wells were assessed using the CyQuant Cell Proliferation Assay Kit (Molecular Probes, #C7026). Seahorse records were corrected for well-to-well differences in the cell numbers and analyzed using Wave software (Agilent) ...
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bioRxiv - Synthetic Biology 2021Quote: ... A small volume of each sample was used to immediately measure protein concentration with the Pierce BCA Protein Assay kit (ThermoFisher; 23227). The rest was combined with 6X loading dye (375mM Tris pH 6.8 ...