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Citations for Thermo Fisher :
7901 - 7950 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Replication activity was examined by using Click-iT™ Plus EdU Flow Cytometry Assay Kits according to the manufacturer’s instructions (C10634; ThermoFisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... and an equal amount of protein was used for labeling by Alexa Fluor 488 using the Click-iT HPG Alexa Fluor 488 protein synthesis assay kit (Thermo Fisher). Labeled nascent proteins were resolved on a 12% SDS gel and visualized on a ChemiDoc Imager (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: Protein contents of the SEC fractions were measured using a BCA protein assay kit (23225, Pierce, Thermo Fisher Scientific, MA, USA). A standard curve (range 0–1000 μg/ml ...
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Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: ... A small aliquot of cell lysate was later thawed and used for total protein quantification following the “BCA Protein Assay Kit” manual (Thermo Scientific). The same quantity (60 μL ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and lysed prior to splitting each sample into two aliquots for D-2-HG quantification and total protein quantification using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 ul of gDNA aliquot was diluted in Qubit BR buffer and sonicated for 15 min before measuring concentrations with the Qubit dsDNA BR assay kit (Invitrogen Q3285). The UHMW gDNA was ready for labeling when the coefficient of variation of the Qubit reads were less than 0.3 ...
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bioRxiv - Cancer Biology 2023Quote: Clear lysates from cells and EVs in 8M urea buffer (8 M urea, 75 mM NaCl, 50 mM Tris) were quantified using microBCA Protein Assay Reagent Kit (Thermo Scientific) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: Intracellular ROS levels from erythroid cells were determined using the CellROX Deep Red Flow Cytometry Assay kit (Invitrogen by Thermo Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: H2O2 contents in the pistil were determined by using the Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit (Invitrogen, catalogue no. A22188). 0.05 g of self- or non-self-pollinated pistils were crushed in liquid nitrogen ...
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bioRxiv - Immunology 2023Quote: ... used to measure AKT activity and the Z’-LYTE Kinase Assay Kit - Ser/Thr 7 Peptide (Thermo Fisher Scientific, Cat# PV3180) used to measure PDK1 activity ...
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bioRxiv - Genomics 2023Quote: ... Then the supernatant was collected and the protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Rabbit polyclonal anti-HMGB2 (1:1,000 ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were fixed with 4% paraformaldehyde and processed according to instructions in the ACD Bio RNAscope Fluorescent Multiplex Assay (Fluorescent Reagent Kit v2) manual and cover slipped with ProLong antifade media (Molecular Probes).
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bioRxiv - Bioengineering 2023Quote: ... Activity of purified uricases and p(Man)-uricase constructs were measured using AmplexTM Red Uric Acid/Uricase Assay kit (Thermo Fisher). Injections were administered once per week intravenously in the tail vein.
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using a BCA protein assay kit (Pierce) and 15-20 μg of protein was separated on a 4-12% NuPAGE Bis-Tris gel (Life Technologies) followed by transfer to PVDF membranes ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA quantity was tested with fluorometry on a DS-11 FX instrument (DeNovix) with the Qubit dsDNA HS Assay Kit (Thermo Fisher). Sizes were analyzed on a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... on ice for 30 min and the protein concentration was determined using the Pierce™ BCA protein assay kits (Thermo Fisher) to ensure equal loading ...
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bioRxiv - Bioengineering 2024Quote: ... 50 μL of supernatant was harvested and assayed for secreted LDH using the CyQuant LDH Cytotoxicity Assay kit according to the manufacturer’s instructions (ThermoFisher, Waltham, MA). The remaining supernatant was collected and frozen at −80° C for subsequent ELISA.
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bioRxiv - Biochemistry 2023Quote: ... The protein in the final sample was quantified using the Qubit Protein Assay Kit with the Qubit 2.0 Fluorometer (Thermo Fisher Scientific).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.05% Trypsin-EDTA (#11580626), Hanks’ Balanced Salt Solution (HBSS, #11560456) and PierceTM BCA Protein Assay Kit (#23227) were bought from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell Data Sciences) and quantified using the Qubit 2.0 Fluorometer with the dsDNA High Sensitivity assay Kit (Q32854, Thermo Fisher Scientific). For each sample ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations of the lysed sEV preparations were quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, USA), following the manufacturer’s protocols (Leung et al. ...
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bioRxiv - Genomics 2023Quote: Intracellular ROS levels from erythroid cells were determined using the CellROX Deep Red Flow Cytometry Assay kit (Invitrogen by Thermo Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Protein concentrations of the soluble cellular protein and 100x concentrated secreted protein samples were quantified against Bovine Serum Albumin (BSA) standards using the Pierce Coomassie Protein Assay kit (#23236, ThermoFisher Scientific). The assays were performed in 96-well transparent plates containing 150 μl of diluted protein sample or 0 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Supernatant was collected for cytokine and proteinase profiling using a custom 22-plex Procartaplex assay (ThermoFisher, #PPX-22-MXRWGMP, custom kit) for the following targets ...
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bioRxiv - Physiology 2024Quote: ... Genomic DNA was extracted from peripheral blood with a salting-out precipitation procedure (Gentra Puregene Blood kit) and CD36 SNP rs3211938 detected using a predesigned TaqMan SNP Genotyping Assay (Applied Biosystems) on a 7900HT instrument ...
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bioRxiv - Genomics 2024Quote: ... DNA was eluted from the column with 26 µL of prewarmed elution buffer and DNA concentrations were assessed with Qubit dsDNA High Sensitivity Assay kit (Invitrogen, Q33231).
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bioRxiv - Genomics 2024Quote: ... The concentration of each sample was then measured using the Qubit dsDNA high sensitivity assay kit (Thermo Fisher Scientific, Cat #Q32854) and the successful removal of adapter dimers confirmed with the 4200 TapeStation system (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentration of each sample was determined using a BCA assay kit (Pierce) and a MULTISKAN microplate reader (Thermo Scientific). Samples (20-25 μg ...
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bioRxiv - Genomics 2024Quote: ... the DNA WGA product was purified with SPRI beads (1.7x) The DNA concentration of each sample was quantified using the Quant-iT® PicoGreen dsDNA assay kit (Invitrogen #P7589). Genomic sequencing library was prepared from 1 ng of purified DNA using the Illumina Nextera XT library preparation kit (Illumina #FC-131-1096) ...
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bioRxiv - Genomics 2024Quote: ... The concentration and integrity of the samples were determined using a Qubit 2.0 fluorometer with the Qubit (ds)DNA HS Assay Kit (Thermo Fisher Scientific) and the Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Plant Biology 2024Quote: ... H2O2 concentration was quantified in the supernatants using the Pierce Quantification Peroxide Assay Kit (23280, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Evolutionary Biology 2024Quote: ... We quantified the final size-selected library pool with the Qubit® Fluorometer with dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: Encapsulation of mRNA in the LNP was measured using a Quani-itTM RiboGreenTM RNA Assay Kit according to the manufacturer’s instructions (ThermoFisher Scientific, R11491). The size ...
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bioRxiv - Molecular Biology 2024Quote: ... The EPR signal intensities were normalized to the protein concentrations of the samples as determined by BCA protein assay kit (Thermofisher ScientificTM).
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bioRxiv - Cell Biology 2024Quote: ... Lysates were spun down at 10,000 RPM and supernatant collected for protein quantification using a Pierce™ BCA Protein Assay Kit (ThermoFisher-23225). NuPAGE™ 4% to 12% ...
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bioRxiv - Neuroscience 2024Quote: ... mRNA-Seq library concentrations (mean of 5.36 ± 2.37 ng μl−1) were measured with the Qubit dsDNA HS Assay Kit (Invitrogen; Cat. No. Q32851) on a Qubit 2.0 Fluorometer ...
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bioRxiv - Molecular Biology 2024Quote: ... the lysates were centrifuged at 16,400 ×g for 15 min at 4 °C and the protein concentration in the supernatants was measured using a Pierce BCA protein assay kit (ThermoFisher Scientific). Total protein (10 µg ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were normalized to total worm extract protein using Pierce™ BCA Protein Assay Kit (Thermo Scientific™; catalog no. 23225). Free fatty acid content were determined by measuring absorbance at 570 nm using BMG Labtech CLARIOstar Plus plate reader.
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bioRxiv - Cancer Biology 2024Quote: ... Lysate supernatants were quantified for protein concentration using the BCA Protein Assay Kit (TermoFisher Scientific, 23225) and separated on 4-20% Tris-Glycine gels (NovexTM, Life Technologies). In the case of WNK1 ...
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bioRxiv - Genetics 2024Quote: ... Samples were quantified using Qubit™ 1X dsDNA High Sensitivity Assay Kit (Catalog number Q33231, Thermo Fisher Scientific, Waltham, MA, USA). DNA quantity and quality were also measured using the Nanodrop ND-1000 Spectrophotometer (Thermo Fisher).
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bioRxiv - Genomics 2024Quote: ... and their concentration and final size distribution were evaluated using a Qubit HS Assay Kit (Thermo Scientific, Hanover Park, IL, USA) and pulse field gel electrophoresis (Pippin pulse ...
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bioRxiv - Microbiology 2024Quote: ... DNA concentration and purity of all samples was measured using a Qubit 4 Fluorometer and the dsDNA BR Assay kit (both Invitrogen, USA)
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bioRxiv - Microbiology 2024Quote: ... DNA concentration of samples post-PCR was measured with the dsDNA BR Assay kit on a Qubit 4 Fluorometer (both Invitrogen, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The protein concentration in the tissue homogenates and EV suspensions was determined with a PierceTM BCA assay kit (Thermo Fisher Scientific) according to the manufacturer’s instruction.
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bioRxiv - Developmental Biology 2024Quote: ... Lysates were subsequently cleared of debris by centrifugation at 14,000g for 15 min and quantified using the BCA protein assay kit (Thermo Fisher Scientific). Protein quantification was performed using the 12-230 kDa plates (ProteinSimple ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate was cleared by centrifugation at 21 130 x g at 4 °C for 30 min and protein content quantified using the Pierce BCA protein assay kit (ThermoFisher Scientfic). Twenty µg of the lysate ...
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bioRxiv - Cell Biology 2024Quote: General protein synthesis levels were measured using the Click-iT Plus OPP Alexa Fluor 488 Protein Synthesis Assay Kit (Invitrogen, C10456). After 48 h of siRNA transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... labeling of newly synthesized DNA was carried out using Click-iT Plus EdU Alexa Flour Flow Cytometry Assay Kit (Thermo Scientific) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA concentration was quantified using a Qubit dsDNA BR assay kit and measured using a Qubit 2.0 fluorometer (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: Protein concentration was determined by a Bradford protein assay using Coomassie plus assay reagent (Fisher Scientific). Optical densities were read on Fluostar (BMG LABTECH ...