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Citations for Thermo Fisher :
7851 - 7900 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... The concentration of proteins in each fraction was determined by a standard colorimetric BCA assay kit (ThermoFisher Scientific, catalogue # 23225). All assays were performed in duplicate on 96 wells plate with an equal quantity of purified protein into each well (200 µg and 2 µg for cytosolic and mitochondrial fractions respectively ...
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bioRxiv - Immunology 2023Quote: Quantification of total protein levels from BAL fluid was measured using a BCA protein assay kit (Thermo Fisher Cat# 23227) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The concentration of each sample pool was measured with Quant-iT PicoGreen dsDNA assay kit (Invitrogen Life Technologies, Stockholm, Sweden) on a fluorescence microplate reader (Ultra 384 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The concentration of each sample pool was measured with Quant-iT PicoGreen dsDNA assay kit (Invitrogen Life Technologies, Stockholm, Sweden) on a fluorescence microplate reader (Ultra 384 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The concentration of each sample pool was measured with Quant-iT PicoGreen dsDNA assay kit (Invitrogen Life Technologies, Stockholm, Sweden) on a fluorescence microplate reader (Ultra 384 ...
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bioRxiv - Genetics 2023Quote: ... and physical titers were measured using a Qubit 3.0 fluorometer and the Qubit dsDNA HS assay kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... the protein concentration of the cell lysates was measured using a Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific). Equal amounts of total protein (1 mg ...
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bioRxiv - Cell Biology 2023Quote: ... The total RNA concentration in the lysates were measured by using Quant-it RiboGreen RNA Assay Kit (ThermoFisher, cat#R11490) and the lysate containing 30 μg of RNA was digested with 60 U of RNase I (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained for 10 min in 0.5 µL/mL calcein-AM and 2 µL/mL ethidium homodimer-1 from the Live/Dead assay kit (L3224, Invitrogen). Then ...
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bioRxiv - Cell Biology 2023Quote: The EdU incorporation assay was performed using the Click-iT Plus EdU cell proliferation kit for imaging (ThermoFisher Scientific #C10638). Third instar wandering larvae (day 7 AEL ...
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bioRxiv - Cancer Biology 2023Quote: Total protein was quantified using the Pierce bicinchoninic protein assay (BCA) kit in accordance with manufacturer’s instructions (23225, ThermoFisher Scientific). Following protein quantification ...
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bioRxiv - Immunology 2023Quote: ... 2% SDS and 10% Glycerol) and protein concentration was measured using Pierce™ BCA Protein Assay kit (Thermo-Fisher Scientific). 20µg of protein was resuspended with SDS-loading dye (50% β-Mercaptoethanol and 0.02% Bromphenolblue) ...
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bioRxiv - Plant Biology 2024Quote: ... The integrity and quality of RNA samples were evaluated using the Qubit RNA IQ Assay Kit (ThermoFisher Scientific, catalog # Q33221) and its concentration were evaluated using Qubit RNA HS Assay Kit (ThermoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the protein concentration in the supernatant was determined using a BCA Protein Assay Kit (Cat# 23225, Thermo Fisher Scientific). Afterward ...
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bioRxiv - Cell Biology 2024Quote: ... scATAC-seq libraries were amplified with 12 and 7 PCR cycles and quantified with the High Sensitivity dsDNA Quanti-iT Assay Kit (ThermoFisher) using a Synergy HTX microplate reader (BioTek ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were transferred to new tubes and protein concentrations were determined using the BCA Protein Assay Kit (Thermo Fisher Scientific). Between 20-50 μg of protein per sample was mixed with Laemmli sample buffer and boiled for 10 min ...
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bioRxiv - Immunology 2024Quote: ... Quantification of the purified amplicons was performed using the Qubit dsDNA HS Assay Kit on the Qubit 3.0 Fluorometer (ThermoFisher Scientific). For the preparation of template-positive Ion PGM™ Template OT2 400 Ion Sphere™ Particles (ISPs ...
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bioRxiv - Neuroscience 2024Quote: ... and the success of the gene editing method was assessed using the T7 Endonuclease 1 (T7E1) assay kit (Invitrogen, Germany). Positive individuals from the resulting F1 generation were then crossed with wild-type fish to produce the F2 generation which were screened again with T7E1 assay ...
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bioRxiv - Microbiology 2024Quote: ... and DNA concentrations were measured using the Qubit™ dsDNA High-Sensitivity assay kit (Thermo Fisher Scientific, cat. no. Q32851).
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bioRxiv - Cancer Biology 2024Quote: ... Isolated DNA was quantified using the Invitrogen™ Qubit™ dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, cat# Q32854). Sequencing libraries were prepared from 10 ng gDNA using the Ion Torrent™ Oncomine™ Comprehensive Assay v3C (OCAv3C ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA concentration was quantified using the Invitrogen™ Qubit™ RNA High Sensitivity Assay Kit (Thermo Fisher Scientific, cat# Q32852). DNA digestion was performed using the Invitrogen™ ezDNase™ enzyme (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA concentration for immunoprecipitated and input control samples were measured with a Quant-iT PicoGreen dsDNA Assay Kit (ThermoFisher Scientific). 5 ng of DNA for each sample was then used with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial cell lysis was determined in cell-free supernatants using the LDH Cytotoxicity Assay Kit (Thermo Fisher Scientific, Waltham, MA). Both DPP4 and LDH activities obtained from cell-free supernatants were normalized to the CFU counts.
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bioRxiv - Molecular Biology 2024Quote: ... Supernatants were transferred to new tubes and protein concentrations were determined by BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). Between 20-50 μg of protein per sample was mixed with Laemmli sample buffer (62.5 mM Tris-HCl (pH 6.8) ...
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bioRxiv - Molecular Biology 2024Quote: ... and the concentration of mini-pools was measured on Qubit (Cat # Q32854; QubitTM dsDNA HS Assay Kit, ThermoFisher Scientificc, US). Equimolar pooling produced a single DNA library ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were quantified using the PierceTM BCA Protein Assay Kit (Pierce Biotechnology, Thermo Fisher Scientific Inc., Waltham, MA, USA). For each sample ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration of the supernatant was determined with the Pierce™ Rapid Gold BCA Protein-Assay-Kit (Thermo Fisher) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2024Quote: ... and 5 the protein concentration of samples was measured with the Pierce® BCA Protein Assay Kit (Thermo Scientific # 23225) and used to normalize metabolite concentrations.
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bioRxiv - Molecular Biology 2024Quote: ... according to the manufacturer’s instructions and quantified using Nanodrop or Qubit RNA BR Assay kit on the Qubit 2.0 Fluorometer (Thermo Fisher Scientific). 1 µg of total RNA was reverse transcribed using SuperScript IV Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Total protein concentration from these tissue homogenate supernatant samples was determined using a BCA protein assay kit (Thermo Fisher Scientific). In brief ...
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bioRxiv - Evolutionary Biology 2024Quote: ... except that the Click-iT™ Plus OPP Alexa Fluor™ 647 Protein Synthesis Assay Kit (ThermoFisher Scientific, Cat#: 10458) was used ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of each gDNA was measured on a Qubit Fluorimeter with the Qubit dsDNA Quantification Assay Kit (Invitrogen, #Q32851). CRISPR sgRNA barcodes were amplified by PCR of up to 10 μg of each gDNA template with a P5 stagger forward primer and P7 barcoded reverse primer [68] ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were purified with AMPure XP beads and eluted in 50 µl 1X TE buffer and quantified using Qubit dsDNA BR Assay Kit and QubitTM 4 Fluorometer (ThermoFisher). Equal amounts of RT-PCR products from three amplicons were pooled together for downstream NGS library preparation and sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... An aliquot (10%) was removed for quantification using the Pierce Quantitative Fluorometric Peptide Assay kit (Thermo Scientific, Cat. No. 23290). The remaining peptides were transferred to autosampler vials (Sun-Sri ...
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bioRxiv - Microbiology 2024Quote: ... The DNA concentrations in the samples were measured using the Qubit® dsDNA HS Assay Kit from Molecular Probes (Invitrogen).
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bioRxiv - Immunology 2024Quote: ... Total protein and dsDNA in the BALF were measured using the Pierce™ BCA Protein Assay Kits (Thermo Scientific, MA) and Qubit™ dsDNA BR Assay Kit (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... cDNA was amplified with 10 cycles and amplified with the Qubit dsDNA HS Assay Kit (Life Technologies, Inc. Cat#Q32851). 0.33ng of each sample was used to construct a pool of uniquely identified samples with the Nextera XT kit (Illumina Cat# FC-131-1096 ...
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bioRxiv - Immunology 2024Quote: Murine IL-5 cytokine measurements of BAL and IL-33 response assay supernatants in Supplementary Figure 5 were performed by ELISA using the mouse IL-5 uncoated ELISA kit (Invitrogen).
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bioRxiv - Developmental Biology 2024Quote: ... We normalized ELISA results to the total protein content assessed using the Pierce™ BCA Protein Assay Kit (Thermo Scientific). For this we measured serial dilutions of the total protein content of respective cultures.
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bioRxiv - Cell Biology 2024Quote: ... Total protein concentration for the detergent soluble fractions were measured with Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) and Biotek Synergy HT plate reader according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... EdU detection was performed using the Click-IT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Life Technologies, C10634). Propidium iodide (PI ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were centrifuged at 16,000rcf for 10 min at 4°C and supernatant was quantified using Pierce Protein BCA assay kit (ThermoFisher Scientific). Samples were diluted in 1X NuPAGE LDS buffer (Invitrogen)/ 2.5% β-Mercaptoethanol and boiled at 70°C for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was assessed for quantity using the fluorescence-based Broad Range Quant-iT RNA Assay Kit (Thermo Fisher, # Q10213) and quality using the Standard Sensitivity RNA Analysis DNF-471 Kit (Agilent ...
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bioRxiv - Immunology 2024Quote: ... Final libraries were quantified for peak size and concentration using the Agilent TapeStation and Qubit HS DNA Assay kit (Invitrogen), respectively.
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bioRxiv - Cell Biology 2024Quote: ... The 20 μg of extracted proteins were quantified using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, WA, USA) at 500 ng/μL ...
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bioRxiv - Neuroscience 2024Quote: ... prior to proceeding with protein quantification using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, cat. no. 23225). 10µg of proteins were resolved using SDS-PAGE (4-12% Bis-Tris precast gels in buffer MES) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration in the tissue lysates were determined by Pierce™ BCA protein assay kit (Thermo Scientific, Cat# 23227, USA). Protein lysates were suspended in sample buffer containing NuPAGE™ LDS Sample Buffer (Invitrogen™ ...
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bioRxiv - Biophysics 2024Quote: ... Protein concentrations were determined using a BCA protein assay kit (Pierce) or by absorbance at 280 nm in a Nano Drop spectrophotometer (ThermoFisher) used in cuvette mode ...
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bioRxiv - Biochemistry 2024Quote: ... The peptide concentration in the HEK293T sample was measured using a Pierce Quantitative Fluorescent Peptide Assay kit (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... cell viability was estimated by calculating the inverse differences in GAPDH Ct relative to the control conditions and the number of viable cells was evaluated using CyQUANT NF Cell Proliferation Assay Kit (Invitrogen).