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Citations for Thermo Fisher :
7851 - 7900 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The coverslips were washed with PBS twice for 5 min each followed by staining with Hoechst 33342 (Invitrogen), for four minutes ...
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bioRxiv - Microbiology 2019Quote: ... The 5′ monophosphate and 3ʹ free –OH ends of the RNA were ligated with T4 RNA ligase (Invitrogen) to make circular molecules ...
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bioRxiv - Microbiology 2020Quote: ... were harvested in an Eppendorf tube and resuspended in 0.5 mL NaCl (0.85%) containing SYTO9 green-fluorescent nucleic acid stain (5 nM final concentration, Invitrogen). Following incubation in the dark for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were pooled (5 μl each) and the pooled products were cleaned using GeneJet PCR purification kit (ThermoFisher). Amplified barcodes were sequenced using an Illumina HiSeq 4000 with Illumina TruSeq primers ...
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bioRxiv - Pathology 2020Quote: ... OCT-embedded frozen specimens were cryosectioned at 5 μm onto charged slides (Fisher Scientific cat# 22-042-924). Slides were air-dried at room temperature overnight and fixed in 10% neutral buffered formalin for 1 hour at room temperature ...
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bioRxiv - Systems Biology 2020Quote: ... we spun down the cells at 1,000 g for 5 min and resuspended in quenching buffer (DBPS, Gibco, 0.1% BSA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Viral RNA was amplified on a Thermal Fisher QuantStudio™ 5 Real-Time PCR System (Thermo Fisher Scientific) using FastStart Universal SYBR Green Master mix (carboxy-X-rhodamine ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4 mM DTT and 20% glycerol) at 30 °C in a Cytation 5 imaging reader (Thermo Fisher Scientific) with filters for excitation at 360/40 nm and emission at 460/40 nm ...
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bioRxiv - Zoology 2020Quote: ... 2’-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-23491-52-3 (Hoechst 33342, trihydrochloride, trihydrate, Life Technologies, H3570) in water for 20 min ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 5 µg of total RNA using Superscript III Reverse Transcriptase (Invitrogen,Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were grown in 1:1 mix of Dulbecco’s Modified Eagle Medium (DMEM) and Ham’s F-12 growth media with 5% Fetal Bovine Serum (FBS) (Gibco) containing L-Glutamine and Phenol Red in 75 mm3 T-75 flasks at 37°C in 5% carbon dioxide (CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... was dissected out using #5 forceps and placed in a new dish containing ice-cold Hibernate A (Gibco) medium without calcium and magnesium ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 μL of purified RNA was added to a reaction mixture containing 1x First Strand buffer (Thermo Fisher), 5 mM dithiothreitol (DTT) ...
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bioRxiv - Microbiology 2021Quote: ... Probes consisting of oligodeoxyribonucleotides (see Supplementary Table 2) were 5′-end labeled using T4 polynucleotide kinase (ThermoFisher Scientific) with 25 μCi of [γ-32P]dATP ...
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bioRxiv - Immunology 2021Quote: ... Array were hybridized with 5 µg of labeled DNA and assayed on a GeneChip Scanner 3000 7G (Affymetrix). Raw data were generated and controlled with Expression console (Affymetrix ...
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bioRxiv - Immunology 2020Quote: ... supernatant was decanted and were then incubated for 5 minutes in RBC lysis buffer (Gibco ACK Lysing buffer) prior to final washing and in vitro assays or flow cytometry.
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bioRxiv - Molecular Biology 2020Quote: ... mESCs expressing mCherry:hCdt and Citrine:Geminin were cultured in 5% CO2 at 37 °C on 0.1% gelatin-coated dishes in DMEM KO (Gibco) media supplemented with 10% FCS ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were passaged 1:2-1:6 every 2-5 days by being rinsed once with DPBS (Gibco) and dissociated using 0.5 mM EDTA (75 μl/cm2 ...
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bioRxiv - Cell Biology 2019Quote: ... 500 ng of each probe were precipitated with 5 μl of Cot-1 DNA (1 mg/ml) (Invitrogen) and resuspended in 15 μl of hybridization buffer (10% dextran sulfate ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were incubated with the permeant sodium indicator sodium green tretraacetate (5 μM, #S6901, Invitrogen, Carlsbad, CA) in complete medium without phenol red ...
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bioRxiv - Cell Biology 2019Quote: ... Genes were quantified using SYBR Green following the manufacturer’s instructions on an QuantStudio 5 qPCR instrument (Applied Biosystems). Gene expression was normalized to an internal control (Rplp0 ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μg/mL gentamicin (Wisent) and 5 ng/mL recombinant mouse fibroblast growth factor basic (FGF-b, ThermoFisher). At ~90% confluence ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA was synthesized from 5 µg of RNA with random primers using Superscript II Kit (Invitrogen, Thermo Fisher). Real-time quantitative PCR was performed with Taqman probes from Applied Biosystems (Hs01568763_m1 for RAD51B ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA was synthesized from 5 µg of RNA with random primers using Superscript II Kit (Invitrogen, Thermo Fisher). Real-time quantitative PCR was performed with Taqman probes from Applied Biosystems (Hs01568763_m1 for RAD51B ...
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bioRxiv - Zoology 2019Quote: Total RNA was extracted from 2-5 larvae using a commercial kit (TRIZOL, Life Technologies, Carlsbad, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: A total of 5 µg RNA was treated with DNase using the TURBO DNA-free kit (Thermo Fisher) and then reverse-transcribed into cDNA using Superscript II or II (Thermo Fisher ...
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bioRxiv - Bioengineering 2020Quote: ... WT-PGP1 were labeled with 5 μM CellTracker™ Blue 7-amino-4-chloromethylcoumarin CMAC (Molecular Probes, #C2110), iNeuron-PGP1 was labeled with 2.5 μM CellTracker™ Green CMFDA (Molecular Probes ...
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bioRxiv - Molecular Biology 2019Quote: ... for 5 minutes and triturated 10x in 1ml RT DNase buffer (0.5g/L DNase, 0.35% Glucose, in BME –GIBCO-) to obtain single cell suspension ...
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bioRxiv - Genetics 2019Quote: Ten week-old mice were killed and PMs were collected by peritoneal lavage with 5 mL PBS (Gibco). The cell suspension was filtered on a 100 µm cell strainer and centrifuged at 800g for 10 minutes at 4°C ...
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bioRxiv - Genomics 2021Quote: ... They were cultured at 37°C with 5% CO2 in McCoy’s 5A medium GlutaMAX™ Supplement (Gibco, 36600021) with 10% FBS (Gibco ...
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bioRxiv - Genomics 2021Quote: ... and 5 ng RNA per reaction in technical duplicate with a StepOnePlus Real-Time PCR system (Applied Biosystems) with SYBR Green detection ...
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bioRxiv - Genetics 2021Quote: ... incubated at 37°C for 5 minutes with Tryple Express dissociation reagent (Fisher Scientific, Cat#: 12-605-010), and collected via scraping ...
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bioRxiv - Cell Biology 2021Quote: HEK293T and SH-SY5Y cell lines were maintained at 37 °C and 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, Biochrom KG) supplemented with 10% fetal bovine serum (Gibco), 100 U ml−1 penicillin (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... the TSCs were plated on 5 µg/ml Collagen IV (corning)-coated dishes with TSCM (DMEM/F12 (Gibco) medium supplemented with 1% ITS-X supplement (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... pDONR221-mBCAR3 was moved into pDEST-5’-3xFlag-pcDNA FRT/TO with a LR reaction (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 minutes prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Physiology 2021Quote: ... at a concentration of 1:500 for 5 min and mounted in SlowFade Diamond Antifade Mountant (ThermoFisher, S36967). Images were captured using a Leica TCS SP5 Confocal Microscope and processed using Fiji (ImageJ ...
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bioRxiv - Cell Biology 2021Quote: ... under standard cell culture conditions (37°C, 5% CO) in Dulbecco’s modified Eagle’s medium (DMEM) high glucose medium (Gibco) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Physiology 2020Quote: ... 600 nM of HIV gag reverse primer and 5 U of Platinum Taq DNA polymerase (Life Technologies; USA). In the 2nd round PCR 5’ LTR region of HIV-1 was amplified using 1 μl of 1st round PCR amplicon as a template ...
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bioRxiv - Molecular Biology 2021Quote: ... and HeLa (ATCC CCL-2) cells were cultured at 37 °C with 5% CO2 in Dulbecco’s modified Eagle’s medium (Invitrogen) supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2021Quote: ... and African green monkey kidney epithelial Vero E6 cells (ATCC) were maintained at 37⍰°C with 5% CO2 in high-glucose Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% FBS (Gibco) ...
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bioRxiv - Neuroscience 2019Quote: The immortalized hippocampal neuron HT22 was cultivated at 37 °C in a humidified 5% CO2 atmosphere and maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) with additions of 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... for microscopy slides or 0.8 mg/ml PEG-Silane and 0.2 mg/ml Biotin-PEG-Silane (5 K, Creative PEGWorks) for coverslips and 0.05 mg/ml NeutrAvidin (Invitrogen) in G-Buffer (2 mM Tris-Cl pH 8 ...
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bioRxiv - Biochemistry 2020Quote: HEK293A and HEK293FT cells were cultured at 37 °C in 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 10% FBS and 100 U/mL penicillin and 100 μg/mL streptomycin ...
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bioRxiv - Biochemistry 2021Quote: ... by heating at 95 °C for 5 min with 2 µL of SDS-PAGE loading dye (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Then pH standard buffers (pH ~6.5 and ~7.5 (0.025 M HEPES, 0.105 M KCl, 0.001 M MgCl2)) were prepared with 10 μM nigericin (Invitrogen, N1495) and added to cells to equilibrate pHe and pHi as previously described.28 For each cell ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... using a capillary BioBasic C18 reversed-phase column (BioBasic-18, 150 × 0.32 mm, 5 μm, Thermo Fisher Scientific), installed in a Dionex Ultimate U3000 HPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... and mouse anti-UBE2J1 (1:250) diluted in blocking solution with 0.1% saponin and 5% goat serum (Gibco) for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: ... filter sterilized using a 5 mL syringe (309646, BD Vacutainer Labware Medical, Fisher Scientific, Hanover Park, IL, USA) with 0.2 µm filter (431219 ...
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bioRxiv - Neuroscience 2021Quote: ... was dissected from embryonic days 13.5 CD1 mouse embryo (50 ganglia per embryo) and incubated with 0.05% trypsin solution containing 0.02% EDTA (Gibco) at 37°C for 15 min ...