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Citations for Thermo Fisher :
7801 - 7850 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... the chemiluminescent signal was elicited by 5 minute incubation with enhanced chemiluminescence substrate (ECL, Pierce, Thermo Fisher Scientific) and captured photographically on film (CL-XPosure ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections of 5 μm were stained for H&E and mounted with Permount Mounting Medium (Thermo Fisher Scientific). For immunohistochemistry ...
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bioRxiv - Cancer Biology 2022Quote: ... and 5 μg of RNA was reverse transcribed using Maxima cDNA Synthesis Kit following manufacturer’s protocol (Fisher Scientific). The resulting cDNA was submitted to PCR analysis using PCRBIO VeriFi™ Mix (PCRBIOSYSTEMS ...
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bioRxiv - Cell Biology 2022Quote: ... and media was replaced with 5 mL complete mTeSR media supplemented with 1X ReVitaCell ROCK Inhibitor (Thermo Fisher). Cells were gently ...
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bioRxiv - Microbiology 2022Quote: ... Between 1 and 5 μg of RNA was boiled at 95 °C with Gel II Loading Buffer (Invitrogen) and loaded on a urea-PAGE gel (Sequagel ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The plates were run on a 384-well QuantStudio™ 5 Real-Time PCR System (Applied Biosystems A28575) using the pre-set comparative-CT-Sybr with melt curve program with the reaction volume adjusted to 10 μL ...
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bioRxiv - Microbiology 2022Quote: For the neutralization assays Vero E6 cells were cultured at 37°C and 5% CO2 in complete Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) containing 10% (vol/vol ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Sections of 5 μm-thick were cut with a microtome before mounted on Superfrost-Plus slides (ThermoFIsher Sci.). Haematoxylin and eosin staining was performed using a Leica Autostainer XL (Leica Biosystems ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... All cell lines were cultured at 37°C with 5% CO2 in DMEM (Thermo Fisher Scientific, MA, USA) with 10% fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: Procyclic forms were cultured at 27°C in MEM-Pros medium [21] supplemented with 5% Penicillin/Streptomycin (Gibco), 7.5 mg/l hemin and 10% of heat inactivated fetal bovine serum at a density between 105 - 4×106 cells/ml.
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bioRxiv - Immunology 2019Quote: ... An eight-point dilution series of patient serum diluted at 1:5 in DMEM (Gibco, Life Technologies, USA) supplemented with 2% FBS were incubated with a constant amount of DENV1 - West Pac 74 and DENV-2 S16803 ...
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bioRxiv - Immunology 2019Quote: ... An eight-point dilution series of patient serum diluted at 1:5 in DMEM (Gibco, Life Technologies, USA) supplemented with 2% FBS were incubated with a constant amount of DENV1 - West Pac 74 and DENV-2 S16803 ...
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bioRxiv - Genetics 2019Quote: ... cDNA was synthesized from 5 µg RNA with 0.5 µg of oligo(dT)12-18 primer (Thermo Fisher) using SuperScript III reverse transcriptase (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2019Quote: ... miR-125b+A or control RNA 3’O-methyl-modified mimic using 5 μl Lipofectamine 2000 (Life Technologies) in a 500 μl reaction mixture according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... T47D:A18 cells and T47D:A18-4HT16 cells were grown in 5% fetal bovine serum (FBS) in RPMI (Life Technologies). T47D:C4217 cells were grown in 5% charcoal stripped calf serum in phenol red free modified RPMI media (Life Technologies) ...
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bioRxiv - Biochemistry 2019Quote: ... 5 mM MgCl2) with 1.5% n-Dodecyl β-D-maltoside (DDM) and 1X Halt Protease Inhibitor (Thermo Scientific) for 30 minutes on ice ...
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bioRxiv - Cell Biology 2019Quote: ... at 4% haematocrit in supplemented RPMI media (RPMI-HEPES, 0.2% NaHC03, 5% heat-inactivated human serum [Australian Red Cross], 0.25% AlbumaxII [GIBCO] ...
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bioRxiv - Synthetic Biology 2019Quote: ... incubated for 5 minutes at RT and transferred to the DynaMag-96 Side Magnet (#12331D, Thermo Fisher Scientific). Following incubation of the sample on the magnet for 2 minutes (or until sample is clear) ...
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bioRxiv - Cell Biology 2019Quote: ... and III (DE) (1.7 kb) of the Cyclin E gene 5’ flanking sequence were synthesized by GeneArt (Invitrogen) with appropriate restriction sites at both ends and subcloned into the pMA plasmid to generate pMA-P ...
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bioRxiv - Neuroscience 2019Quote: Total RNA from fly heads was isolated from 5-day-old female flies with TRIzol reagent (Invitrogen, USA). Reverse transcription of RNA to cDNA was achieved using 2 μg of total RNA with random hexamer primers using RT-PCR SuperMix Kit (TransGen Biotech ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were then treated with ExoSAP-IT enzyme (2 μl of enzyme for 5 μl of sample, ThermoFisher) at 37 °C for 15 min followed by a 15 min inactivation at 80 °C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and 2 pmol of each primer (5’ primers labeled with Applied Biosystems fluorescent dyes FAM, HEX or NED). Thermal cycling (under mineral oil ...
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bioRxiv - Cell Biology 2020Quote: ... at 37°C and 5% CO2 with 6 mL HDF medium consisting of 1x Advanced DMEM (Gibco™), 1x Glutamax™ (Gibco™) ...
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bioRxiv - Neuroscience 2019Quote: ... rinsed again (3 × 5 min) in 1X PBS and mounted using SlowFade™ Diamond Antifade Mountant (Invitrogen; S36963). Fluorescence images were acquired with a confocal laser scanning microscope (LSM 700 or 710 ...
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bioRxiv - Cell Biology 2019Quote: ... for 10 minutes, and fixed with methanol:glacial acetic acid, (5:1, v/v) (FisherThermo Fisher Scientific, Rockford, IL). Cells were dropped onto slides ...
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bioRxiv - Microbiology 2019Quote: ... PUUV-Gc-rev 5’-ATTCCCGGGCTTGTGCTCCTTC-3’ to PCR amplify (Phusion Flash High-Fidelity PCR Master Mix, ThermoFisher Scientific) the inserts from codon optimized PUUV GPC described in Iheozor-Ejiofor et al. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Germany) were cultivated in DMEM and HAM’s F12 containing 5 % heat-inactivated fetal calf serum (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... adult AB zebrafish olfactory rosettes were dissected and placed directly into 5 ml of Neurobasal medium (Gibco, 21103049) supplemented with 10% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2020Quote: ... 5-ethynyl-2’-deoxyuridine (EdU)) staining were performed using the Click-iT Plus EdU Assay kit (C10339; Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... HEK293T and THP-1 cells were cultured in an incubator at 37C and 5% CO2 in Dulbecco’s modified Eagle’s Medium (DMEM) containing phenol red (Invitrogen) or Rosewell Parks Memorial Institute (RPMI ...
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bioRxiv - Neuroscience 2019Quote: ... in Iscove’s modified Dulbecco’s medium (IMDM) containing 5% fetal calf serum using TurboFect transfection reagents (Thermo Fisher Scientific). 16 hours after transfection ...
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bioRxiv - Developmental Biology 2019Quote: ... Chromatin was pre-cleared with 5 or 10 µl of 1:1 protein A:G Dynabeads (Life Technologies #10015D) and immunoprecipitated with H3K4me1 (Abcam #ab8895) ...
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bioRxiv - Molecular Biology 2020Quote: ... Stock cultures were grown in medium containing 5 mL of RPMI 1640 (GIBCO BRL, Gaithers-Burg, MD, USA) supplemented with 5 % fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2019Quote: ... Enzymatic digestion was inhibited by addition of perfusion buffer containing 5 % (v/v) FBS (FBS; Thermo Fisher, UK). Isolated cardiomyocytes were reintroduced to Ca2+ by three rounds of 20 minutes sequential gravity settling in perfusion buffer containing 300 μM ...
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bioRxiv - Bioengineering 2020Quote: ... The supernatant was harvested 5 days after transfection and purified with a CaptrueSelect KappaXL Affinity Matrix (Thermo Scientific) followed by size exclusion chromatography on a Superdex200 Increase column (GE Healthcare).
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bioRxiv - Genetics 2020Quote: ... and in some cases day 5 (120 hours) by staining with SYBR Green 1 nucleic acid stain (Invitrogen, ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... for 1-3h at 37°C in a HERAcell 150i or 160i 5% CO2 incubator (Thermo Fisher Scientific). Cells were washed in 1x PBS and fixed in 10% neutral buffered formalin (HT501128 ...
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bioRxiv - Microbiology 2020Quote: ... falciparum were maintained at 5-10% parasitaemia in RPMI1640 supplemented with 0.5% Albumax II (RPMI 1640-Albumax; ThermoFisher) (Trager and Jensen,1976) ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNAs were synthesized from 5 µg total RNA using the SuperScript II first-strand cDNA synthesis system (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were grown under standard tissue culture conditions (37°C, 5% CO2) in RPMI medium (GIBCO Cat# 31870025), supplemented with 10% FBS ...
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bioRxiv - Plant Biology 2021Quote: ... The bands were detected by using SuperSignal pico/femto chemiluminescence kits (Thermo Scientific; #’s1859022/3 and 1859674/5).
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bioRxiv - Cancer Biology 2019Quote: ... harvested after 4-5 days and counterstained with Near-IR fluorescent Dye (Live/Dead Cell Stain Kit, Invitrogen) to determine the number of viable dividing (CFSE/NearIR- ...
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bioRxiv - Systems Biology 2019Quote: ... The RNA was fragmented for 5 min at 95°C in the presence of Superscript III buffer (Invitrogen) followed by a five-minute incubation on ice ...
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bioRxiv - Cancer Biology 2020Quote: Cells were maintained at 37 °C under humidified 5% CO2 in DMEM medium supplemented with 10% FCS (Invitrogen) and 2 mM glutamine ...
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bioRxiv - Molecular Biology 2021Quote: ... At least 400 μl of purified erythrocytes were rapidly lysed in 5 volumes pre-warmed TRIzol (ThermoFisher Scientific) and stored at −80°C until further processing.
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were grown at 37°C with 5% CO2 and passaged using 0.05% Trypsin-EDTA solution (Thermo Fisher) or TryplE Express Enzyme (Thermo Fisher).
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bioRxiv - Neuroscience 2021Quote: ... cells were washed with PBS(−/−) three times and then blocking buffer (5% normal goat serum [Cat #PCN5000, ThermoFisher] ...
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bioRxiv - Systems Biology 2019Quote: ... followed by another centrifugation (1000 g, 5 min) and resuspension in 0.5 ml of PBS-BSA 0.01% + RiboLock (ThermoFisher) (0.8 U/µl) ...
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bioRxiv - Systems Biology 2019Quote: ... followed by another centrifugation (1000 g, 5 min) and resuspension in 0.5 ml of PBS-BSA 0.01% + RiboLock (ThermoFisher) (0.8 U/µl) ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was prepared by in vitro transcription using T7 RNA polymerase (5 U/μl final, Thermo Fisher Scientific) at 37 °C for 4 hours in the presence of 100 ng/μl digested plasmid ...