Labshake search
Citations for Thermo Fisher :
551 - 600 of 1270 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2024Quote: ... Monosaccharides in hydrolysates were separated by a CarboPac PA-20 column (3 × 150 mm; Thermo Fisher Scientific) using a program described previously71 ...
-
bioRxiv - Cell Biology 2024Quote: ... and medium was replaced with 150 µL complete DMEM containing 50 nM Lysotracker Deep Red (L12492; Invitrogen) and incubated at 37°C for 4 hr ...
-
bioRxiv - Cell Biology 2020Quote: ... PCR products were run on 1.5-2% agarose gel containing 1μl/ml SYBR safe DNA gel stain (expected band~ 150 bps) (ThermoFisher).
-
bioRxiv - Cell Biology 2020Quote: ... a 0.075 × 150 mm-EASY-Spray column (3-μm particle diameter, 100 Å pore size, Thermo Fisher Scientific) with mobile phases of 0.1% formic acid and 0.1% formic acid/80% acetonitrile was used ...
-
bioRxiv - Microbiology 2021Quote: ... and purified proteins (2.5 μg in 25 μl HEPES pH 7.5 150 mM NaCl) were incubated with 25 μM BocillinFL (Invitrogen) for 20 min at 37°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... The cell pellets were lysed with 1 mL lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol, 0.5% NP-40, 1:200 SUPERase• In [Invitrogen] ...
-
bioRxiv - Cell Biology 2022Quote: ... equipped with an Accucore C30 column (150 × 2.1 mm, 2.6 µm) and its 20mm guard (Thermo Fisher Scientific). Samples were kept at 8°C in the autosampler ...
-
bioRxiv - Plant Biology 2020Quote: ... All connections were of PEEK material with an internal diameter of 150 μm (nanoViper, Dionex; Thermo Fisher Scientific). The outlet from the DAD detector was connected to an ICP-MS (Agilent 8800 ICP-QQQ-MS ...
-
bioRxiv - Microbiology 2021Quote: ... Proteins were diluted to a final concentration of 0.1 mg/mL in 150 mM citrate phosphate buffers ranging from pH 3.0 to 7.5 containing 5x SYPRO Orange (Invitrogen). SYPRO Orange fluorescence was monitored over a 25°C to 80°C temperature gradient (30 s increments ...
-
bioRxiv - Biochemistry 2020Quote: ... then back-eluted onto an Acclaim PepMap 100 C18 nanocolumn (0.75 mm × 150 mm, 2 μm, Thermo Scientific). Separation of peptides on the chromatographic system was performed using a binary gradient of solvent A (0.1% formic acid in water ...
-
bioRxiv - Cell Biology 2021Quote: ... Stable cell lines were selected with 150 µg/ml hygromycin and 15 µg/ml blasticidin (Thermo Fisher Scientific). Individual cell clones were induced with 1µg/ml doxycycline for 24 hours and expression of the USP7 proteins assessed by immunoblotting and fluorescence microscopy ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were loaded onto a PepMap 100 C18 column (0.3 mm × 150 mm, 2 μm, Thermo Fisher Scientific). Separation of the samples was performed using mobile phase A (0.1% formic acid in water ...
-
bioRxiv - Cell Biology 2021Quote: ... coated glass bottom dishes and stained with TMRE (1: 3000 dilution from 150 μM stock solution, Invitrogen T669), BTX-Alexa Fluor 488 (1 ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were incubated after permeabilization with either BODIPY 493/503 (0.5 μg/ml in 150 mM NaCl; ThermoFisher), Nile Red (1:8000 in 150 mM NaCl ...
-
bioRxiv - Cancer Biology 2022Quote: ... 3.3 μg DNA of either plasmids was added to 150 μl Opti-MEM reduced serum media (Gibco #31985062) then mixed thoroughly with 9.9 ul Fugene HD transfection reagent (Promega #E2311) ...
-
bioRxiv - Molecular Biology 2022Quote: ... run at 150 V for 90 minutes in 1× NuPAGE Tris-Acetate SDS Running Buffer (Thermo Fisher Scientific). Proteins were electrotransferred onto an Immobilon-fl polyvinylidene difluoride (PVDF ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... the total protein content from each replicate (150 μg) was separated on precast polyacrylamide gels (Thermo Fisher Scientific). Each lane was cut into 12 slices and in-gel protein digestion was performed (Shevchenko et al ...
-
bioRxiv - Microbiology 2022Quote: ... samples were incubated at 58 °C for 1 hour and 150 μl of buffered phenol (Invitrogen, 15513-047) was added ...
-
bioRxiv - Biochemistry 2022Quote: ... Human embryonic kidney cells (HEK293) were cultured in culture flask (150 cm2) to 80 % confluence in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) supplemented with 50 units/ml penicillin G (Gibco) ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were grown in P-150 cm cell dishes and fixed with 1% methanol-free formaldehyde (Thermo Scientific) for 10 min at room temperature ...
-
bioRxiv - Systems Biology 2021Quote: ... Samples were loaded directly onto Acclaim Pepmap® C18 (75 μm × 150 mm, 2 μm) (Thermo Scientific, USA) analytical column at a flow rate of 1 μL/min for 15 minutes in 5% acetonitrile ...
-
bioRxiv - Neuroscience 2022Quote: ... internal solution was supplemented with 20-150 μM Alexa Fluor 488 and/or Alexa Fluor 594 (Life Technologies).
-
bioRxiv - Microbiology 2020Quote: ... Samples were loaded onto a PepMap 100 C18 column (0.3 mm × 150 mm, 2 μm, Thermo Fisher Scientific). Separation of the samples was performed using mobile phase A (0.1% formic acid in water ...
-
Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... using a capillary BioBasic C18 reversed-phase column (BioBasic-18, 150 × 0.32 mm, 5 μm, Thermo Fisher Scientific), installed in a Dionex Ultimate U3000 HPLC system (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2021Quote: ... An EASY-Spray column ES802A (150 mm long, 75 μm i.d., 3 μm C18/silica particles, ThermoFisher Scientific) was used at flow rates of 250 nL/min ...
-
bioRxiv - Biochemistry 2021Quote: ... The lipids were separated using a C30 Accucore LC column (ThermoFisher Scientific, 150 mm * 2.1 mm * 2.6 µm) using the following mobile phases ...
-
bioRxiv - Developmental Biology 2021Quote: ... 150 μl of yellow-green fluorescent 2.0 uM polystyrene microspheres (FluoSpheres carboxylate modified microspheres (2% solid solution, Invitrogen) and 50 ul of deionized water ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 150 µg protein was proteolyzed using trypsin (Pierce Trypsin Protease, MS-grade; Thermo Scientific, Waltham, MA, USA). Surrogate peptides and internal standard peptides were precipitated using triple X proteomics (TXP ...
-
bioRxiv - Cell Biology 2023Quote: HeLa cells were grown at 37 °C in an atmosphere of 5% CO2 and 95% air on 150 cm2 tissue culture flasks in Dulbecco’s Modified Eagle’s Medium (Invitrogen) containing 10% SVF ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were washed in cold PBS and then lyzed in 150 μl of TRIzol reagent (Thermo Fisher Scientific) added to the apical side of the insert ...
-
bioRxiv - Cell Biology 2023Quote: ... The siRNAs were transfected into HeLa cells stably expressing Halo–mGFP–Golgi using Lipofectamine RNAiMAX (13778-150; Invitrogen), in accordance with the manufacturer’s instructions ...
-
Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: ... 10 µL tetramethylethylenediamine (TEMED) and 150 µL 10 wt% ammonium persulfate (APS) casting into mini-cassette (ThermoFisher Novex). Loading wells were generated by inserting the comb into the cassette.
-
bioRxiv - Developmental Biology 2023Quote: ... were isolated from embryos and placed into 500 μL or 150 μL pre-warmed trypsin solution (2.5 μg.mL-1 trypsin (Gibco), 25 mM Tris ...
-
bioRxiv - Immunology 2023Quote: Cells or extracted mitochondria were lysed by sonication in RIPA lysis buffer (25 mM Tris-HCl, pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, ThermoFisher) containing Minitab protease inhibitors (Sigma) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: Lung organoids in Col I gels were digested with 150 U/ml collagenase type I (Gibco, in IMDM) for 1 h at 37°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... dissociated PDTOs were incubated with 150 μg/mL of mouse anti-human HLA-ABC (clone W6/32, ThermoFisher) to block MHC-I and 30 μg/mL mouse anti-human HLA-DR/DP/DQ (clone Tu39 ...
-
Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... Total RNA (150 ng) was amplified using the WT PLUS Reagent Kit (Thermo Fisher Scientific Inc., Waltham, USA). Amplified cDNA was hybridized on Human ClariomS arrays (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2024Quote: ... then lysed with 1ml of polysomal buffer (20 mM Tris, pH 7.4, 150 mM NaCl, 1.25 mM MgCl2, 5 U/ml RNase inhibitor [Invitrogen] ...
-
bioRxiv - Molecular Biology 2024Quote: ... 150 - 300 ng of pcDNA3.1-LINC01918_201 or pcDNA3.1-empty vector were transfected using Lipofectamine LTX (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... Polyadenylated RNA fragments (150 – 500 nt) were enriched with Dynabeads™ mRNA Purification Kit (Thermo Fisher, Vilnus, Lithuania) and used with the mixture of specially designed primers (SWITCH2 and RT_universal_primer ...
-
bioRxiv - Immunology 2024Quote: ... cells were labelled with 150 μM of the fluorescently labeled glucose analog 2-NBDG (Thermo Fisher Scientific, N13195) or with 1µM bodipy (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... 1.2 mM EDTA, 0.01% SDS, 150 mM NaCl. 1.1% Triton and 1x Halt protease inhibitor cocktail from ThermoFisher). To reduce non-specific background ...
-
bioRxiv - Systems Biology 2024Quote: ... 15 cm × 150 μM internal diameter) by a Dionex RSLCnano ultra-performance liquid chromatography (UPLC) system (ThermoFisher Scientific). Buffer A comprised water with 0.1% (vol/vol ...
-
bioRxiv - Biochemistry 2023Quote: ... and the supernatant was incubated with 150 µL of a slurry of Dynabeads MyOne Streptavidin C1 (Invitrogen, 65002) pre-bound to 75 pmol of capture oligonucleotide (Table S2) ...
-
bioRxiv - Immunology 2022Quote: ... Mice were imaged 5 minutes after intraperitoneal injection with fresh d-luciferin (150 mg/kg; Thermo Fisher Scientific). Luciferase signal was quantified using Living Image software (Caliper LifeSciences ...
-
bioRxiv - Microbiology 2022Quote: ... centrifuged at 1500 rpm for 5 minutes at 4°C and lysed with RIPA lysis buffer (25mM Tris HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS) (ThermoFisher) supplemented with protease inhibitor cocktail (Sigma) ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1.5-2 µg of mRNA was fragmented to 100-150 nts using RNA Fragmentation Reagent (Thermo Fisher Scientific) followed by overnight ethanol precipitation ...
-
bioRxiv - Neuroscience 2022Quote: Extracted metabolites were re-suspended in 150-200 μl of Optima UPLC/MS grade water (Thermo Fisher Scientific). After 15 min incubation on a thermomixer at 4°C and a 5 min centrifugation at 21100 x g at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... 150 mM NaCl) supplemented with 1% Triton X-100 v/v and protease inhibitor cocktail (ThermoFisher J61852.XF). Bacteria were lysed by sonication (20 pulses ...
-
bioRxiv - Microbiology 2022Quote: ... 150 mg of filter (sand) material was mixed with 125 µL of B-PER reagent (78243, Thermo Scientific) and 125 µL 1 M TEAB buffer (50 mM TEAB ...