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Citations for Thermo Fisher :
601 - 650 of 1270 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... mice were administered a dorsal subcutaneous 150 µL injection of 30 mg/mL D-luciferin (Thermo Fisher Scientific) reconstituted in PBS and luminescence images was captured 15 minutes thereafter ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Hypoxia: Cells were incubated in DMEM at 1% O2 using a HERAcell 150.i CO2 incubator (Thermo Scientific).
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bioRxiv - Cell Biology 2024Quote: ... the cells were plated at 20% confluence in complete DMEM supplemented with 150 μg/mL hygromycin B (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: Collections of timed embryos were placed in a 0.5 ml Eppendorf tube to which 150 µl Trizol (Ambion) was added ...
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A novel male accessory gland peptide reduces female post-mating receptivity in the brown planthopperbioRxiv - Neuroscience 2024Quote: Total RNA from 150 virgin male accessory glands was isolated three days post-emergence using TRIzol reagent (Invitrogen), following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... cells were re-plated in media without zeocin and with 150 ug/mL hygromycin B (Gibco™ 10687010) for selection ...
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bioRxiv - Microbiology 2024Quote: ... and growth was monitored at 37°C in 100-well (150 µL-well) honeycomb plates (Thermo Scientific, #12871511), using a Bioscreen C Analyzer (Thermo Labsystems) ...
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bioRxiv - Systems Biology 2024Quote: 150 human islets were hand-picked on the day of distribution and placed in 1ml of Trizol (Invitrogen), vortexed and stored at −80°C until shipping to Oxford or Stanford for further processing ...
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Polyunsaturated Fatty Acid - mediated Cellular Rejuvenation for Reversing Age-related Vision DeclinebioRxiv - Molecular Biology 2024Quote: Separation of lipids was performed on an Accucore C30 column (2.6 μm, 2.1 mm × 150 mm, Thermo Scientific). The Q Exactive MS was operated in a full MS scan mode (resolution 70,000 at m/z 200 ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were transfected the next day with a mixture of 150 µL of Opti-MEM (Gibco, 31985-070), 9 µL FuGENE ® HD Transfection Reagent (Promega ...
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bioRxiv - Bioengineering 2024Quote: ... Then 100 µL lysis buffer (10 mM MES, 150 mM NaCl, pH 6.0, B-PER II (Thermo Fisher), 50 U mL-1 Benzonase ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were transfected with pGL4.34-2X-T-Resp (150 ng/well) and pGL4.73[hRluc/SV40] (7.5 ng/well) plasmids using Lipofectamine 3000 (Invitrogen). Non-transfected cells and a single transfection with 150 ng pGL4.34-2X-T-Resp were included as negative controls ...
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bioRxiv - Cell Biology 2024Quote: Separation of lipids was performed on an Accucore C30 column (2.6 μm, 2.1 mm × 150 mm, Thermo Scientific). The Q Exactive MS was operated in a full MS scan mode (resolution 70,000 at m/z 200 ...
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bioRxiv - Developmental Biology 2024Quote: ... and each pair of ovaries was digested in 150 µl of 0.25% trypsin-EDTA (Fisher Scientific cat# 25200056) in 1.5-ml Eppendorf tubes at +37°C for 20 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein extraction occurred following cell lysis by adding 150 µL Pierce® radioimmunoprecipitation assay (RIPA) buffer (Thermo Fisher Scientific) and 1.5 µL protease and phosphatase inhibitor cocktail (10X Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: Sperm were isolated from 2-4 wild-type and mutant male fish and kept in 150 μl Hank’s saline containing 0.5 μM MitoTracker Deep Red FM (Molecular Probes) for >10 minutes on ice ...
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bioRxiv - Cell Biology 2020Quote: Near-confluent (80-90%) MEFs were transfected with 150 nM lamellipodin or Rac1 siRNAs using Lipofectamine 2000 reagent (Invitrogen) in reduced serum Opti-MEM as previously described [1 ...
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bioRxiv - Developmental Biology 2021Quote: Spermatozoa were squeezed from 2–4 wild-type and mutant male fish and kept in 150 µL Hank’s saline containing 0.5 µM MitoTracker Deep Red FM (Molecular Probes) for >10 minutes on ice ...
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bioRxiv - Molecular Biology 2021Quote: For siRNA transfection experiments: HEK293 cells were grown in 6-well plates and RNAimax transfection reagent (Invitrogen #13778-150) was used following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... 150 µg of protein extract from each sample were ran on NuPAGE 4%–12% Bis–Tris Protein Gels (Invitrogen) using MES running buffer (100 mM MES ...
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bioRxiv - Genomics 2020Quote: ... transfected 24 h after seeding with 150 ng of base editor and 50 ng of gRNA expression plasmid and 0.5 µl of Lipofectamine 2000 (Invitrogen) per well ...
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bioRxiv - Microbiology 2021Quote: ... BN-PAGE gels were run for 2 to 2.5 h at 150 V using NativePAGETM running buffer (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... with 150 µl of sample in black glass-bottom 96-well plates (Nunc™ #165305 96-Well Optical Bottom). Fluorescence reads were obtained with excitation at 380 nm and emission at 440 nm both with 20 nm bandwidth ...
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bioRxiv - Bioengineering 2020Quote: ... Cardiomyocyte cells were seeded on 0.5% gelatin-coated multiwell plates at 80-150 } 103 cells/cm2 and cultured in DMEM containing 4.5g/L glucose (Gibco) supplemented with 17% M-199 medium (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... End labeling was carried out overnight at room temperature in TdT buffer containing 0.5 μM DIG-dUTP and 150 U/ml TdT (Invitrogen). Embryos were then washed for 2 hours at 65°C in PBS/1 mM EDTA ...
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bioRxiv - Cell Biology 2021Quote: ... The 100 µm capillary column was packed with 35 cm of Accucore 150 resin (2.6 μm, 150Å; ThermoFisher Scientific). The scan sequence began with an MS1 spectrum (Orbitrap analysis ...
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bioRxiv - Biochemistry 2020Quote: Cells were thawed in Buffer A (50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP (Thermo Fisher), 5% glycerol ...
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bioRxiv - Neuroscience 2020Quote: ... were kept on dry-ice whilst 150 µL of ice-cold methanol (OptimaT LC-MS, Fisher Scientific; LOT: 1862991) was added to each retina sample with a multichannel pipette ...
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bioRxiv - Plant Biology 2021Quote: ... The sample was reconstituted in 150 µL 25% (v/v) acetonitrile and filtered using a micropore filter (0.22 µm, 0.75 ml, Thermo scientific) prior to ultra-high performance liquid chromatography – tandem mass spectrometer (UHPLC–MS/MS ...
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bioRxiv - Microbiology 2020Quote: ... 50 µl cold lysis buffer (50 mM Tris HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% Triton X100, 10 µl/ml HALT protease and phosphatase inhibitor [ThermoFisher]) was added and cells were lysed by bead beating using glass beads ...
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bioRxiv - Cancer Biology 2020Quote: ... Stained cells were washed and resuspended in 150 μL FACS buffer and 80μL of samples were acquired on an Attune flow cytometer (ThermoFisher). Human CD4+ and CD8+ T cells were gated based on live/hCD45+mCD45−hCD3+hCD4+hCD8− and live/hCD45+mCD45−hCD3+hCD4−hCD8+ respectively.
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bioRxiv - Biophysics 2021Quote: ... LMNG followed by 20 column volume of Buffer B (25 mM HEPES pH 7.4, 150 mM NaCl) supplemented with 0.06% (w/v) Digitonin (ACROS Organics). TMEM120A was eluted in Buffer B with 0.06% (w/v ...
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bioRxiv - Cancer Biology 2021Quote: ... 4T1 cells or Py8119 cells suspended at 1-2 million/150 µl of 33% (v/v) matrigel (Fisher Scientific) were transplanted subcutaneously to the right flank of 6-8 week-old BALB/c or C57BL/6J female mice ...
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bioRxiv - Physiology 2022Quote: Urogenital ridges of E12.5 embryos carrying the GOF18-GFP reporter were isolated and placed into 150 μl of trypsin solution (2.5 μg/ml trypsin (Gibco), 25 mM Tris ...
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bioRxiv - Physiology 2022Quote: ... 10 µL of hydrolysate were mixed with 150 µL of isopropanol followed by 75 µL of 1.4% chloramine-T (ThermoFisher) in citrate buffer (pH = 6.0 ...
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bioRxiv - Cell Biology 2022Quote: ... equipped with an Accucore C30 column (150 x 2.1 mm, 2.6 μm) and its 20mm guard (Thermo Fisher Scientific). Samples were kept at 8°C in the autosampler ...
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bioRxiv - Cell Biology 2022Quote: ... using 150 ng of plasmid and 1.5 µl of Lipofectamine 2000 per 100 µl Opti-MEM (ThermoFisher Scientific, 31985062). Imaging was performed at 37°C in pre-warmed Leibovitz medium (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... all samples were immediately buffer exchanged into an nMS-compatible solution (150 mM ammonium acetate, pH 7.5, 0.01% Tween-20) with a 40 kDa MWCO (ThermoFisher Scientific). For nMS analysis ...
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bioRxiv - Developmental Biology 2022Quote: ... Selection was applied to transfectants for at least 5 passages prior to use: 150 µg/ml hygromycin-B (ThermoFisher) and 1 µg/ml puromycin (ThermoFisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... 150 μl per well of this mixture (1500 cells per well) was plated in 96-well white plates (Nunc), whose wells had been previously coated with poly(2-hydroxyethyl methacrylate ...
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bioRxiv - Developmental Biology 2021Quote: ... used at 1:5,000 in MAB buffer (100 mM Maleic acid, 150 mM NaCl, pH7.5) + 10% heat-inactivated lamb serum (Gibco 16070096) + 2% blocking reagent (Sigma 11096176001) ...
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bioRxiv - Cell Biology 2021Quote: ... and lysed in lysis buffer (150 mM KCl, 20 mM Hepes, pH 7.4, 1mM MgCl2, 0.1% Triton, HALT Protease Inhibitor Cocktail (ThermoFisher Scientific), 40 units/ml Ribolock RNase Inhibitor (ThermoFisher Scientific)) ...
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bioRxiv - Microbiology 2021Quote: Samples were analyzed using a Hypersil GOLD C18 column (2.1 mm × 150 mm, 1.9 μm particle size; Thermo Fisher) attached to a Thermo Scientific Dionex UltiMate 3000 UHPLC instrument in line with an LTQ-OrbiTrap XL instrument (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2021Quote: ... A range of 1,000–11,000 cells per well was seeded into wells prefilled with 150 μL Essential 6™ (E6 medium) (Gibco, Carlsbad ...
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bioRxiv - Physiology 2022Quote: 150 islets from chow-fed ZMIZ1Ctrl and ZMIZβ1KO mice were lysed in 1 mL of TRIzol Reagent (ThermoFisher Scientific) and RNA was extracted as per manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2022Quote: ... Supernatant was removed and pellets were then resuspended in 1 mL IP buffer (50 mM Tris Base, 150 mM NaCl, 0.5 M EDTA, pH 7.4) with PierceTM protease (Thermo Scientific) and PierceTM phosphatase (Fisher ...
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An engineered receptor-binding domain improves the immunogenicity of multivalent SARS-CoV-2 vaccinesbioRxiv - Microbiology 2020Quote: ... BN-PAGE gels were run for 2 hours at 150 V using the NativePAGE™ running buffer (Life Technologies) according to the manufacturer’s instructions.
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bioRxiv - Pathology 2020Quote: ... fitted with a C30 column (Acclaim 2.1 × 150 mm, 3 μm particle size, Thermo Fisher Scientific, Scoresby, VIC, Australia) held at 10 °C ...
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bioRxiv - Microbiology 2021Quote: ... coli BW25113 cells and an appropriate mutant strain was used to inoculate 150 ml of Lennox LB (Fisher Scientific) and was cultivated to an OD578 of 0.5-0.6 at 37°C before harvesting by centrifugation (4500 × g ...
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bioRxiv - Molecular Biology 2021Quote: ... the medium was replaced with 1.75 ml fresh proliferation medium and the transfection mixture was prepared: A solution 150 µl Optimem (Gibco, Thermo Fisher Scientific ...