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Citations for Thermo Fisher :
751 - 800 of 1016 citations for Corning Kanamycin Sulfate Powder since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: Samples from time course experiments and the truncation screening were prepared for sodium dodecyl-sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) by adding 12 µL of NuPAGE LDS Sample Buffer (Thermo Fisher Scientific) and 3 µL of tris(2-carboxyethyl)phosphine (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... The cells were selected for three passages in DMEM supplemented with 10% FCS and 500 μg/mL G418 sulfate (ThermoFisher Scientific, 10131027). The extent of overexpression was assessed with flow cytometry (Suppl ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by sodium dodecyl sulfate Bis-Tris polyacrylamide gel electrophoresis (SDS-PAGE) (NuPAGE™ 4 to 12%, Bis-Tris precast polyacrylamide gel, Invitrogen # NP0323BOX) and afterward transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2023Quote: ... Beads were washed twice with the 1% NP-40 lysis buffer and eluted by boiling in sodium dodecyl sulfate (SDS) sample buffer (Thermo Fisher Scientific) and subjected to western blot analysis.
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bioRxiv - Microbiology 2023Quote: ... aeruginosa and Salmonella organisms were prepared and separated by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) as described (18) with a Novex X-Cell Surelock minicell system (Invitrogen, Carlsbad, CA). Tris-bis-polyacrylamide gels (12.5% ...
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bioRxiv - Biochemistry 2023Quote: ... beads were washed four times in MCLB buffer supplemented with 150 mM NaCl and then boiled in LDS (lithium dodecyl sulfate) sample buffer (Thermo Fisher, NP0008) supplemented with beta-mercaptoethanol to elute the bound proteins.
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bioRxiv - Neuroscience 2022Quote: ... positive selection of transfected astrocytes was performed by treating cells for 36 h with Geneticin® (G-418 Sulfate; Thermo Fisher, 11811023) 100 μg/mL in complete culture medium ...
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bioRxiv - Genomics 2022Quote: ... 2.5% dextran sulfate, 0.2% BSA, 100 μg/mL salmon sperm DNA, 2X SSC, 0.1 U/μL SUPERase•In™ [Thermofisher] in DEPC H2O) was then pipetted into the chamber ...
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bioRxiv - Cell Biology 2022Quote: ... 20 μl RIPA buffer was used per mg of tissue and the RIPA buffer was completed by addition of 0.1% sodium dodecyl sulfate (Thermo Fisher Scientific, BP166), 0.5% sodium deoxycholate (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated using Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) in 8% polyacrylamide gel together with PageRuler protein ladder (Thermo Fisher Scientific) and transferred onto polyvinylidene difluoride membrane (PVDF) ...
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bioRxiv - Molecular Biology 2024Quote: ... 6-8-week-old WT mice were randomly selected to receive 2% dextran sodium sulfate (DSS) 40-50,000 MW (Affymetrix, Santa Clara, CA) in drinking water for 7 days followed by normal drinking water afterwards ...
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bioRxiv - Microbiology 2023Quote: ... and their purity validated by sodium dodecyl sulfate -polyacrylamide gel electrophoresis (SDS-PAGE) followed by Western blot (WesternBreeze Chromogenic Immunodetection System, Thermo Fisher Scientific) using antibodies against His6xtag.
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bioRxiv - Neuroscience 2022Quote: ... Aliquots were thawed on ice and then denatured in a sample buffer (containing: 500mM DTT, lithium dodecyl sulfate [LDS]) with Coomassie G250 and phenol red (Invitrogen™ NuPAGE) at 65 °C for 10 min ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were separated by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in either homemade 7% or 4-12% gradient Bis-Tris polyacrylamide gel (Thermo Fisher Scientific) using 3-(N-morpholino)propanesulfonic acid (MOPS ...
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bioRxiv - Bioengineering 2022Quote: ... Proteins were separated by 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride (PVDF) membrane (Thermo Fisher Scientific). The membranes were blocked in 5% nonfat milk (RPI International ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were separated by dodecyl-sulfate polyacrylamide gel electrophoresis and transferred to Amersham™ Protran® nitrocellulose membranes or PVDF membranes (Invitrogen). The membranes were blocked with 5% non-fat dry milk-TBST (10 mM Tris-HCL [pH 8.0] ...
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bioRxiv - Bioengineering 2024Quote: ... sodium dodecyl sulfate (SDS) (Fischer Scientific, Fair Lawn, NJ) with 0.5% 10,000 U/mL penicillin streptomycin (PS) (Gibco, Life Technologies, Grand Island, NY) for 4-5 days to fully decellularize based on previously established criteria (1) ...
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bioRxiv - Cell Biology 2024Quote: ... cells from the 6-well plate were harvested in ice-cold 1X DPBS using cell scrapers and lysed in 1X lithium dodecyl sulfate (LDS) sample buffer (Life Technologies, NP0007) supplemented with 100 mM dithiothreithol (DTT ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein purity was assessed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Laemmli, 1970) and staining (PageBlueTM solution from Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... The culture media was supplemented with 10% fetal bovine serum (HyClone) and 1% penicillin/streptomycin sulfate (Cat# 15140, Life Technologies, Carlsbad, CA). The neuronal excitability was assessed 15─24 h after plating the neurons.Whole-cell current-clamp recordings ...
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bioRxiv - Immunology 2024Quote: 50 μg exosomes were incubated with 5 μm aldehyde/sulfate latex beads (4% w/v, 4 µm, Invitrogen™, Massachusetts, United States) in a 100 μl final volume of DPBS and incubated for 15 min on a shaker at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Millipore Sigma)-coated Corning BioCoat 96-well plates (Thermo Fisher). Human induced motor neurons were then cultured in motor neuron culture media (Neurobasal-A with N2 (1:100)/ B27 (1:50)/ Glutamax (1:100)/ ×1 NEAA (1:100)/penicillin-streptomycin (1:100) ...
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bioRxiv - Bioengineering 2020Quote: ... 6-well culture plates (Corning; Thermo Fisher Scientific), and overlaid with glass coverslips to aid in tissue adherence ...
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bioRxiv - Cell Biology 2021Quote: ... with 10% fetal bovine serum (FBS, Corning/Gibco). LNCaP cells were maintained in RPMI-1640 (Corning/Cellgro 10-040-CV ...
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bioRxiv - Microbiology 2022Quote: ... sodium bicarbonate (2.1g/liter, Corning by ThermoFisher Scientific), HEPES (15 mM ...
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bioRxiv - Immunology 2020Quote: ... Corning 96-well half area plates (ThermoFisher 3690) were coated with 1μg/mL of antigen overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... a cover glass (Corning No. 1, Fisher Scientific) was dipped overnight in PLL solution (Poly-L-lysine hydrobromide powder ...
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bioRxiv - Cell Biology 2022Quote: ... 6-well culture plates (Corning; Thermo Fisher Scientific), and digested for 2 min with 0.25% trypsin/0.5 mM EDTA (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... sodium bicarbonate (2.1g/liter, Corning by ThermoFisher Scientific), HEPES (15mM ...
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bioRxiv - Cell Biology 2023Quote: ... 30% Ham’s-F12 (v/v) (Fisher Scientific Corning), 2% B27 (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... 30% Ham’s-F12 (v/v) (Fisher Scientific Corning), 2% B27 (v/v ...
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bioRxiv - Genetics 2024Quote: ... coated Corning™ (Fisher Scientific, #07-200-82) Costar™12 well plate ...
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bioRxiv - Immunology 2024Quote: Culture Media: RPMI-1640 + L-glutamine (Corning-Gibco), Penicillin Streptomycin L-glutamine (Life Technologies) ...
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bioRxiv - Bioengineering 2024Quote: ... were seeded on Corning Matrigel (354277, Fisher Scientific) coated six-well plates ...
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bioRxiv - Cancer Biology 2020Quote: Isolated colonic crypts were embedded in Corning® Matrigel® Matrix Corning™ Matrigel™ GFR Membrane Matrix (Fisher Scientific CB40230C) and seeded onto pre-warmed 24-well plates (CytoOne ...
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bioRxiv - Cancer Biology 2024Quote: ... SNU-499 and Hep3B cells were seeded on Corning Costar low-attachment 96-well plates (Corning-Costar, ThermoFisher Scientific, Stockholm, Sweden) in decreasing serial dilutions (100-3 cells/well) ...
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bioRxiv - Plant Biology 2024Quote: ... Samples (10 mg powder) were extracted threefold in ice-cold 90% (v:v) methanol (LC-MS grade; Fisher Scientific UK Limited or Th. Geyer GmbH & Co. KG), supplemented with luteolin 7-O-glucoside (Extrasynthese ...
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bioRxiv - Bioengineering 2021Quote: ... sodium dodecyl sulfate (SDS) (Fischer Scientific, Fair Lawn, NJ) with 0.5% 10,000 U/mL penicillin streptomycin (PS) (Gibco, Life Technologies, Grand Island, NY) until fully decellularized based on previously established criteria65 ...
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bioRxiv - Bioengineering 2022Quote: ... or fluorescent oligonucleotide probes/constructs (10-50 nM in PBS with 0.025% T20, 300 mM NaCl, 0.5 mg/mL sheared salmon sperm DNA [SSS DNA, ThermoFisher] and 0.5% dextran sulfate [Sigma]) for 30 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Equal protein loads were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and proteins were transferred to nitrocellulose membranes (Thermo Scientific, Dreieich, Germany). The following primary antibodies were used at the indicated dilutions ...
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bioRxiv - Cell Biology 2021Quote: ... and the soluble fractions were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on 6% Tris-Glycine gels (Life Technologies, Carlsbad, CA)64 ...
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bioRxiv - Microbiology 2021Quote: ... and then stacked in a single band in the top of a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel (4%–12% NuPAGE gel, Invitrogen, Illkirch-Grafenstaden/France). After staining with R-250 Coomassie Blue (Biorad ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (EMD Millipore; Thermo Fisher Scientific). PVDF membrane was treated with primary and secondary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... Precipitates were lysed in cell lysis buffer (20 mM Tris/HCl, pH7.5; 1xLithium Dodecyl Sulfate sample buffer (Thermo Fisher Scientific, Waltham, MA, USA); a protease inhibitor cocktail (cOmplete ...
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bioRxiv - Molecular Biology 2023Quote: ... was maintained in a humidified incubator at 37°C in 5% CO2 in αMEM supplemented with 10% fetal bovine serum (FBS) and gentamicin sulfate (10 μg/ml) (Life Technologies, Carlsbad, CA).
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bioRxiv - Cancer Biology 2023Quote: ... 1% antibiotic-antimycotic (AA: penicillin 100 U/mL, streptomycin sulfate 100 U/mL, 412 amphotericin B 0.25 mg/mL; Gibco, Cat. no. 15240-062), and 1% non-essential amino acids (NEAA ...
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bioRxiv - Microbiology 2023Quote: ... 20μg of protein was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on NuPAGE 4-12% Bis-Tris gels (Thermo Fisher Scientific #NP0329). The gel was blotted onto Immobilon-FL PVDF Membrane (Millipore #IPFL00010 ...
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bioRxiv - Microbiology 2023Quote: ... The crude cell extract was cleared by centrifugation at 9700xg (8,000 rpm) for 50 min at 4°C prior to 23% ammonium sulfate precipitation (Fisher Scientific, Fair Lawn, NJ). The technical aspects of ammonium sulfate precipitation were very important ...
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bioRxiv - Cancer Biology 2023Quote: Total cell lysates were collected from GBM control and EMP3 KO cells lysed with 1% sodium dodecyl sulfate and 1% sodium deoxycholate in 100 mM triethylammonium bicarbonate (TEAB; Thermo Fisher Scientific PI90114) containing protease and phosphatase inhibitors ...
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bioRxiv - Microbiology 2024Quote: ... proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoreses (SDS-PAGE) and stained using GelCode™ Blue Safe Protein Stain (Thermo Fisher Scientific). The specific activities of diaphorase and NTR were typically in the range of 100–150 and 3–5 μmol/min/mg ...