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Citations for Thermo Fisher :
701 - 750 of 1016 citations for Corning Kanamycin Sulfate Powder since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... cell pellets were lysed via bead beating in presence of 2% sodium dodecyl sulfate (SDS) and an aliquot from each sample was digested using Pierce MS grade trypsin (Thermo Fisher), labeled with TMT 10plex isobaric tags (Thermo Fisher) ...
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bioRxiv - Bioengineering 2021Quote: ... once with a 1% SDS-TRIS pH 11 solution (5% v/v of 20% sodium dodecyl sulfate #05030, from Sigma Aldrich and 2.4 % w/v TRIS base PBP151-500 from Fisher Scientific in reverse osmosis water ...
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bioRxiv - Microbiology 2020Quote: ... Enriched virus was inactivated with 2% sodium dodecyl sulfate (SDS) and protein concentration determined with a Pierce BCA protein assay kit (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Protein lysates of HAP1 and HeLa cells were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4-12% gradient precast polyacrylamide gels (Life Technologies). The proteins separated by SDS-PAGE were transferred onto a nitrocellulose membrane using a Mini-Protean® Tetra Cell device ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using NuPAGE Bis-Tris gels (Thermo Fisher Scientic) and transferred on nitrocellulose membranes (iBlot 2® gel transfer Stacks Nitrocellulose system from Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Hamster lungs were homogenized and diluted in Hank’s solution with the addition of 2% FCS and antibiotics (streptomycin sulfate and benzylpenicillin sodium salt, 200 U/ml) (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) before transferring onto 0.45 μm nitrocellulose membrane (Thermo Scientific™) with a Hoefer TE77X semi-dry transfer unit at 20V for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... magnetically isolated for a final time and then boiled in Laemmli buffer (62.5 mM Tris-HCl, 10% glycerol, 2% sodium dodecyl sulfate and 0.01% bromophenol blue (B392, Fisher Scientific, Pittsburgh, PA) for 5 minutes to release antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... and working solutions of lysates at concentrations of 15-30 µg total proteins per 30 µl were prepared with 1× lithium dodecyl sulfate (LDS) sample buffer with reducing reagent (NuPAGE, ThermoFisher Scientific) followed by heating at 70 °C for 20 min on a Thermomixer (Eppendorf) ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were heated in LDS (lithium dodecyl sulfate) loading buffer then loaded into 4-12% bis-tris gels (Thermo Scientific #NW04122BOX). When blotting for DNA-PKcs ...
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bioRxiv - Microbiology 2020Quote: HAP- or ΔXKR8-produced purified rVSV/G or rVSV/EBOV-GP (1.5×109 genomes) were conjugated to 4-μm aldehyde/sulfate latex beads (Thermo Fisher Scientific) overnight at 4°C with gentle shaking ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cells were lysed in Laemmli buffer and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to a polyvinylidene fluoride membrane (ThermoFisher, 88518). Membranes were blocked with 5% milk in TBST for 1h ...
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bioRxiv - Immunology 2020Quote: Single cell splenocytes and lung cells suspensions were resuspended in buffered ammonium sulfate to lyse erythrocytes and then washed and resuspended in RPMI 1640 (Life Technologies) supplemented with 10% heat-inactivated foetal bovine serum (Scientifix ...
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bioRxiv - Immunology 2022Quote: ... Samples were heated in LDS (lithium dodecyl sulfate) loading buffer then loaded into 4–12% bis-tris gels (Thermo Scientific #NW04122BOX) followed by transfer to a PVDF membrane ...
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bioRxiv - Microbiology 2022Quote: ... Equal protein loads were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and proteins were transferred to nitrocellulose membranes (Thermo Scientific). For protein detection the following primary antibodies were used at the indicated dilutions ...
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bioRxiv - Microbiology 2022Quote: ... resuspended in sodium dodecyl sulfate (SDS) lysis buffer (1% SDS 10 mM EDTA 50 mM Tris, pH 8.1) containing protease inhibitors (Thermo Scientific, #EO0492), and incubated on ice for 20 min ...
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bioRxiv - Physiology 2024Quote: ... Twenty-five µg of protein were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Fisher Scientific). Western blot analysis was performed with the following antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were embedded in a thin polyacrylamide gel and were then treated with a digestion buffer of 2% v/v sodium dodecyl sulfate (SDS; ThermoFisher, AM9823), 0.5% v/v Triton X-100 (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Equal protein loads were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and proteins were transferred to nitrocellulose membranes (Thermo Scientific). For protein detection the following primary antibodies were used at the indicated dilutions ...
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bioRxiv - Immunology 2023Quote: ... Integrity and purity of purified proteins were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and Coomassie G-250 staining (ThermoFisher Scientific). Purified proteins were stored at –80°C until use.
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bioRxiv - Biochemistry 2023Quote: ... The purity of the ATAD2/B bromodomain was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels stained with GelCode Blue Safe protein stain (Thermo Scientific) (24,25).
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bioRxiv - Cancer Biology 2023Quote: ... GFP+ TC2 cells were cultured in complete media supplemented with 1 mg/ml Geneticin (G418 Sulfate) (11811023; ThermoFisher, Waltham, MA, USA). Cells were cultured at 37°C with 5% CO2.
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bioRxiv - Molecular Biology 2023Quote: ... with 30,000 viable cells replated on 10-cm dishes in media supplemented with 1 mg/ml G418 sulfate (Gibco, Billings, MT). After two weeks of selection with G418 ...
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bioRxiv - Neuroscience 2023Quote: ... 15 µg of total protein was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using a 10-20% tris-glycine gradient (Invitrogen, XP10205BOX), transferred to a PVDF membrane using the Trans-Blot Turbo Transfer System (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... lysates were separated by sodium dodecyl sulfate/polyacrylamide (SDS/PAGE) gel electrophoresis on 1 mm NuPAGE 3 – 8% gradient Tris-Acetate gels (Thermo Fisher) using Tris-Acetate SDS running buffer and transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellets were lysed in 10% sodium dodecyl sulfate (SDS) containing 100 mM triethylammonium bicarbonate (TEAB) with Pierce protease inhibitor (ThermoFisher Scientific) supplemented ...
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bioRxiv - Biophysics 2023Quote: ... Proteins and lipids in the SEC fractions were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on pre-cast 4-12% gradient gel (Life technologies) and by LC-MS/MS ...
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bioRxiv - Biophysics 2024Quote: ... affinity captured GEMs were eluted from beads by addition of 20 μl of 1x LDS (lithium dodecyl sulfate) loading buffer (Thermo Fisher) and vortexing for 10 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... and run at 150 V for 1 hr with NuPAGE MOPS sodium dodecyl sulfate (SDS Running Buffer, 20X, Thermo Scientific Inc). Following electrophoresis ...
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bioRxiv - Cell Biology 2024Quote: ... were infected at 30-50% confluence for 24 h and then selected under 0.4 mg/mL Geneticin/G418 sulfate (Gibco, 11811-023) for an additional 5 days ...
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bioRxiv - Microbiology 2024Quote: ... The cell monolayers were washed three times with DMEM (without antibiotics or FBS) prior to treatment with 100 µg/mL Gentamicin Sulfate (Fisher Scientific) for 1 h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... samples were incubated in Tyramide development solution (2% Dextran sulfate, 0.0015% hydrogen peroxide, 0.2mg/ml Iodophenol, 1:100 Alexa Fluor 594 Tyramide Reagent (Thermo Scientific, Cat. # B40957) in PTw ...
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bioRxiv - Synthetic Biology 2024Quote: ... were solubilized in 10% β-mercaptoethanol (BME) in 4x lithium dodecyl sulfate (LDS) sample buffer and resolved on Bolt Bis-Tris Plus gels (ThermoFisher). The samples were later transferred to immobilon PVDF transfer membranes and blocked with 5% milk (w/v ...
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bioRxiv - Plant Biology 2024Quote: ... Phages were harvested by disrupting the overlay agar with 6 ml of SM buffer (50 mM Tris-HCl, 8 mM magnesium sulfate, 100 mM sodium chloride, 0.01% gelatin, pH 7.5; Thermo Scientific, J61367-AK), followed by centrifugation at 8000 g for 10 min and filtration of supernatant via 0.2 µm PES syringe filters ...
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bioRxiv - Immunology 2024Quote: 1 - 5 µL aliquots of eluted MOMP-NLPS were mixed with 4x NuPAGE lithium dodecyl sulfate sample buffer and 10x NuPAGE sample reducing agent (Life Technologies), heat-denatured at 95°C for 5 minutes and loaded onto a 4-12% gradient premade 1.0 mM Bis-Tris gel (Life Technologies ...
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bioRxiv - Genomics 2024Quote: ... The samples were then digested at 42oC for 48 hours in digestion buffer (2% v/v sodium dodecyl sulfate [SDS] (Thermo Fisher), 1% v/v proteinase K (New England Biolabs) ...
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bioRxiv - Biophysics 2024Quote: Single colonies of relevant strains were inoculated into ∼3 ml Brain Heart Infusion (BHI) media (Remel) with 120 µg/ml spectinomycin sulfate (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were separated by sodium dodecyl sulfate– polyacrylamide gel electrophoresis (SDS-PAGE) Novex 4-20% Tris-Glycine gel (Thermo Fisher Scientific) and transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Bioengineering 2020Quote: ... freeze-dried mycelium was ground to a fine powder using mortar and pestle under liquid nitrogen and RNA was extracted with TRIzol™ Reagent (Invitrogen; Art. No.: 15596026) according to the manual ...
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bioRxiv - Microbiology 2022Quote: THY medium was made using the premixed powders of Todd Hewitt Broth (BD Bacto Dehydrated Culture Media: Todd Hewitt Broth, Fisher Scientific, DF0492-17-6) and yeast extract (Thermo Scientific™ Yeast Extract ...
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bioRxiv - Microbiology 2024Quote: ... The 4 × 6 tin cup packaging samples were used to weigh approximately 0.1 mg of powder sample and then subjected to the isotope ratio mass spectrometer (IRMS, Thermo Fisher Scientific Inc., Waltham, USA) for stable isotope determination in Third Institute of Oceanography (Xiamen ...
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bioRxiv - Plant Biology 2023Quote: ... The infected leaves were ground into powder using liquid nitrogen and total RNA was extracted using TRIzol Reagent (Thermo Fisher Scientific, Cat. No. 15596018) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Cyclophosphamide solution was prepared immediately prior to a single IP injection by dissolving cyclophosphamide monohydrate powder (Cat. No. AC203960250, Fisher Scientific, Hampton, NH, USA)7-9 in sterile saline (0.9% ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked using 5% non-fat milk powder diluted in TBST (Tris-buffered Saline, Bio-Rad, Cat # 1706435, supplemented with 0.1% Tween-20, Thermo Fisher Scientific, Cat # 28320) for 4 hours at room temperature ...
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bioRxiv - Immunology 2021Quote: ... and final product purity were assessed on a 15% sodium-dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using SYPRO Ruby Protein Gel Stain (S12000, Thermo Fisher Scientific). Refolding efficacy was measured by denaturing XCL1 with 20 µM beta-mercaptoethanol (-ME) ...
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bioRxiv - Bioengineering 2021Quote: ... sodium dodecyl sulfate (SDS) (Fischer Scientific, Fair Lawn, NJ) with 0.5% 10,000 U/mL penicillin streptomycin (PS) (Gibco, Life Technologies, Grand Island, NY) until fully decellularized based on previously established criteria65 ...
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bioRxiv - Cell Biology 2022Quote: ... It was then diluted to a final concentration of 25nM in either 1X or 4X NuPAGE™ Lithium dodecyl sulfate Sample Buffer (Thermo Fisher) and incubated for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... 30 µL of 10% sodium dodecyl sulfate (SDS) 100 mM triethylammonium bicarbonate (TEAB) with Pierce protease inhibitor cocktail (ThermoFisher Scientific, Waltham, MA) and phosphatase inhibitors (10 mM sodium pyrophosphate ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... CHO cells stably transfected with the PAR biosensors cloned into pcDNA3.1(+) were cultured in complete F-12 medium with 600 µg ml-1 geneticin selective antibiotic (G418 Sulfate, Gibco® ThermoFisher Scientific). The cells were grown in a T75 cell culture flask (Nunc ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... CHO cells stably transfected with the PAR biosensors cloned into pcDNA3.1(+) were cultured in complete F-12 medium with 600 µg ml-1 geneticin selective antibiotic (G418 Sulfate, Gibco® ThermoFisher Scientific). The cells were grown in a T75 cell culture flask (Nunc ...