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Citations for Thermo Fisher :
651 - 700 of 1016 citations for Corning Kanamycin Sulfate Powder since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: Human HT1080 cells were cultured in DMEM supplemented with 10% FBS (Labtech) plus 100 U/ml penicillin G and 100 μg/mL of streptomycin sulfate (Invitrogen). Cells were grown at 37 °C in 5%CO2 in a humidified atmosphere ...
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bioRxiv - Cell Biology 2020Quote: A total of 20 μg of protein was processed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with a 4-12% Bis-Tris Mini-gel (Invitrogen) and the 3-(N-morpholino ...
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bioRxiv - Developmental Biology 2021Quote: ... and then diluted to 0.5-2 mg/ml in the hybridization solution with 5% dextran sulfate (Cat. No. S4030; Thermo Fisher). Fluorescence RNA in situ hybridization procedure was performed following Thisse and Thisse (2008 ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% sodium deoxycholate and 0.1% sodium dodecyl sulfate (SDS)) containing Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (Thermo scientific). Loading buffer and reducing agent (both Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... of cell lysate proteins per lane were resolved on SDS-PAGE (8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis) along with pre-stained protein ladder and transferred onto PVDF membranes (Invitrogen). The transferred membranes were blocked for 1 hour using 5% BSA in TBST (25 mm Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... The proteins in the resulting pellets were then dissolved in 100 μL LDS 1X (Lithium dodecyl sulfate) sample buffer (Invitrogen) and supplemented with 5% beta-mercaptoethanol (vol/vol ...
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bioRxiv - Bioengineering 2023Quote: ... Protein gels were used to check for the presence and purity of protein and run using sodium dodecyl sulfate polyacrylamide gradient gels (NuPage 4−12% Bis-Tris gel from Invitrogen) using the Precision Plus Dual Color protein standard (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... slides were thawed at room temperature for 1 hr and then placed in 15mls of 1% UltraPure Sodium Dodecyl Sulfate (SDS) solution (cat# 15553035; Invitrogen) for 5-10 minutes with agitation (40 rpm) ...
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bioRxiv - Neuroscience 2023Quote: ... were conducted to remove trace PFA followed by 5 min antigen retrieval with SDS (1% Sodium Dodecyl Sulfate; Fisher Scientific) in PBS ...
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bioRxiv - Immunology 2023Quote: ... resuspended in solubilization buffer (9M Urea containing 1% DTT and 2% Triton X and NuPAGE lithium dodecyl sulfate sample buffer 4X (Invitrogen) at a 3:1 ratio) ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µg of each sample were subjected to Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS/PAGE) using precast 10% Bis-Tris NuPage Gels (Invitrogen), transferred onto PVDF membranes ...
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bioRxiv - Molecular Biology 2024Quote: Protein samples in lauryl-dodecyl sulfate sample buffer (LDS) were separated by standard SDS-PAGE on 4-12% Bis-Tris gradient gels (Novex Bolt, Invitrogen). Separated proteins were transferred onto PVDF membranes (Milipore ...
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bioRxiv - Developmental Biology 2023Quote: Protein extracts were separated by 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (Invitrogen). Blocking and antibody incubations were performed in 5% bovine serum albumin ...
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bioRxiv - Microbiology 2024Quote: ... were mixed with 4X Sample Buffer (“4X SB”: 250 mM Tris pH 6.8, 2% Dodecyl sulfate sodium salt (Acros Organics), 20% β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2023Quote: ... 10-20µg of protein was loaded per well and run on a 4-12% bis-tris precast sodium-dodecyl sulfate page gel (NP0323BOX, Invitrogen). Semi-dry transfer was performed using the TransBlot Turbo transfer system (1704150 ...
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bioRxiv - Cell Biology 2023Quote: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed with 8% or 10% acrylamide gels and BOLT MES (Invitrogen) or Tris-glycine SDS running buffer ...
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bioRxiv - Neuroscience 2023Quote: ... were conducted to remove trace PFA followed by 5 min antigen retrieval with SDS (1% Sodium Dodecyl Sulfate; Fisher Scientific) in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Fourteen μL of clarified lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 10% polyacrylamide Bis-Tris gels (Invitrogen) and transferred onto Immobilon FL PVDF membranes (Millipore ...
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bioRxiv - Immunology 2022Quote: ... resuspended in solubilization buffer (9M Urea containing 1% DTT and 2%Triton X and NuPAGE lithium dodecyl sulfate sample buffer 4X (Invitrogen) at a 3:1 ratio) ...
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bioRxiv - Cancer Biology 2023Quote: ... Sodium dodecyl sulfate–polyacrylamide gel electrophoresis and Western blotting were performed using pre-cast NuPAGE Bis-Tris 4 - 12% mini-gels (Invitrogen) with 1X MOPS buffer (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... the samples were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using the Mini Gel Tank electrophoresis system (Invitrogen). Proteins were denatured in a loading buffer (4X LDS sample buffer +β-mercaptoethanol ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled for 5 min and subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on either 10% gels (cast freshly) or 4-20% gradient gels (Invitrogen). Proteins were transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% sodium dodecyl sulfate) containing protease and phosphatase inhibitors (Pierce Protease Inhibitor Mini Tablet, EDTA-free, Thermo Scientific; PhosSTOP, Roche) for 45 minutes at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... alginate and calcium sulfate solution were either directly deposited into an 8-well Lab-Tek chamber slide (Thermo Fisher Scientific) or onto a hydrophobic glass plate which was then covered with another glass plate with a 1 mm spacer between plates ...
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bioRxiv - Microbiology 2023Quote: ... These saturated YPD cultures were used to inoculate SD minimal medium (6.7 g/L Difco Yeast Nitrogen Base without amino acids but with ammonium sulfate from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Equal concentrations of protein were prepared under reducing conditions and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (4–20% Tris Glycine 1.0mm Mini Protein Gels from Invitrogen XP04200BOX). Gels were transferred to PVDF membranes using the iBlot™ 2 transfer stacks (ThermoFisher IB24002) ...
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bioRxiv - Plant Biology 2024Quote: ... Protein concentration was determined using Bradford Assay and the cleared lysate was mixed with lithium dodecyl sulfate loading buffer (ThermoFisher) and DTT to a final concentration of 200 mM and then boiled at 90°C for 10 min ...
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bioRxiv - Immunology 2024Quote: ... resuspended in solubilization buffer (9M Urea containing 1% DTT and 2% Triton X and NuPAGE lithium dodecyl sulfate sample buffer 4X (Invitrogen) at a 3:1 ratio) ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and detected using Streptavidin-HRP (Thermo Fisher) or anti-LC serum as previously described.
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bioRxiv - Neuroscience 2024Quote: ... Eight µg of protein were loaded on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gels (NuPAGE Novex 4-12% Bis-Tris mini or midi, NP0335 or WG1402BOX, Invitrogen) and run for 1 h 40 min at 100 V ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were then put under selection by supplementing the media with 200μg/ml G418 Sulfate (Thermo Fisher Scientific, #10131035). Positive cells were subsequently transduced with a construct expressing KRAB-dCas9 linked to BFP under a TRE3G promoter (Addgene ...
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bioRxiv - Bioengineering 2024Quote: ... An equivalent amount of total protein was loaded into Precise Tris-HEPES 4–20% sodium dodecyl sulfate (SDS)-polyacrylamide gels (ThermoFisher) and separated by electrophoresis ...
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bioRxiv - Biochemistry 2024Quote: ... The purity of the CECR2-BRD was confirmed through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) stained with GelCode Blue Safe protein stain (ThermoFisher).
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bioRxiv - Biophysics 2021Quote: MDCK-II wild type canine kidney epithelial cells were a gift from the Nelson Laboratory at Stanford University and were cultured in customized media consisting of low-glucose (1 g/L) DMEM with phenol red (Gibco, USA; powder stock), 1 g/L sodium bicarbonate (lower than standard DMEM) ...
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bioRxiv - Plant Biology 2021Quote: ... The plant tissues and cell pellets were ground into fine powder with liquid nitrogen and the RNAs were prepared using TRizol (Thermo Fisher, Waltham, USA) according to the manufacture’s protocol and followed by DnaseI digestion (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... This served as the H2O-based media for experiments and the D2O-based media was made to an identical composition using RPMI media powder (Thermo Fisher Scientific, #31800022) and heavy water (Sigma-Aldrich ...
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bioRxiv - Genomics 2023Quote: Total RNA was extracted from 80 mg of frozen kidney (n=15) and liver tissue powders (n=15) in 1mL Trizol reagent (Invitrogen, #Ref 15596-018) and isolated from Nucleospin® RNA II kit (Macherey-Nagel ...
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bioRxiv - Plant Biology 2023Quote: ... The epidermal peels were ground to a fine powder in liquid nitrogen using mortar and pestle and RNA isolated via TRIzol (Thermo Scientific, Karlsruhe, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... plates were blocked with PBS-T containing 0.5% Omniblok non-fat milk powder (AmericanBio, Cat No. AB10109-01000) and 3% goat serum (Gibco, Cat No. 16210-072) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: Frozen thymic tumor tissue was ground to a fine powder with a mortar and pestle and lysed in TRI ReagentTM Solution (ThermoFisher Scientific, cat#AM9738) using a microtube-sized Dounce homogenizer ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was blocked in 5% milk powder in PBS and incubated with primary antibodies (1:1000 rabbit α-Apaf1 SY22-02 (Invitrogen, MA5-32082) and 1:200 mouse α-beta-actin (Sigma ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: Turbidity was achieved using dissolved kaolin clay powder (Figure 1b) and it was measured using a turbidity meter (Thermo Scientific Orion AQUAfast AQ3010) before and after each trial with increased turbidity ...
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bioRxiv - Biophysics 2021Quote: ... CellTak (Corning Cell-Tak; Fisher Scientific, Germany) was diluted in 0.1 M NaCO3 and 1 M NaOH ...
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bioRxiv - Biophysics 2022Quote: ... 1× Corning nonessential amino acids (Fisher Scientific), 1x Corning MEM vitamin solution (Fisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... 1x Corning MEM vitamin solution (Fisher Scientific), 0.67 mM L-arginine ...
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bioRxiv - Cancer Biology 2024Quote: ... Soluble fibronectin (Fisher Scientific Corning ™; 356008) was added to the medium for 24 hours at 100 μg/ml after diluting it in mFAD with 25mM Hepes (Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... Base media of RPMI/B27 (Corning, Gibco) without insulin was used until Day 7 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were diluted in 6X Laemmli sample buffer and resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Thermo Scientific). Blots were probed with the indicated primary antibodies and with horseradish peroxidase (HRP ...
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bioRxiv - Immunology 2021Quote: ... 5×105 murine or human T cells have been stimulated at 1:1 ratio in 48well plate in presence of Sulfate Latex 4% w/v 5µm Beads (Molecular Probes S37227). For each experiment ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μg of each sample was separated in 8% sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (45-004-110, Fisher Scientific) in ice-cold transfer buffer (25 mM Tris-base ...