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Citations for Thermo Fisher :
501 - 550 of 1016 citations for Corning Kanamycin Sulfate Powder since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... The harvested cells and /or tissue powder was dissolved in RIPA buffer containing Halt™ Phosphatase (Thermo Scientific, # 78420) and Protease Inhibitor Cocktail (Roche complete tablets mini EASYpack ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were applied for 2 hours in PBS/3% milk powder containing 1 μg/ml Hoechst-33342 (Invitrogen) or DAPI (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... The tissue powder was transferred to 2 mL microcentrifuge tube and 1 mL TRIzol Reagent (ThermoFisher, Cat no 15596018) was added ...
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bioRxiv - Plant Biology 2022Quote: ... the membrane was then incubated for 1 h in a blocking solution composed of 0.5 % semi-skimmed milk powder in TBST (Tris-buffered saline 20 mM, 0.1 % Tween 20) and then incubated with the anti-glutathione (Invitrogen) primary antibody solution at 4 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... beads were resuspended in 1 mL of 100 mM sodium borate (pH 9.0) followed by the addition of dimethylpimelimidate powder (DMP, ThermoFisher) to a final DMP concentration of 25 mM ...
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bioRxiv - Bioengineering 2024Quote: ... Graphitene Energy Storage Series https://www.graphitene.com) were mixed with 1.289 g of Zeolite Y powder (ThermoFisher Scientific UK, 045862.36). The thickness of graphene powder was 1-3 layers with a lateral size 0.5 – 5 μ m ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was extracted from 100mgs of tissue powder using GeneJet Plant RNA purification mini kit (Thermo scientific, USA), and the concentration of RNA was measured using a nanodrop (Thermo scientific ...
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bioRxiv - Cell Biology 2023Quote: MCF-10A cells (ATCC) and derivatives were cultured in Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 Ham 1:1 powder (Gibco) supplemented with 5% horse serum (Invitrogen) ...
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bioRxiv - Biophysics 2024Quote: ... Stanford) were cultured in media consisting of low-glucose (1 g/L) DMEM with phenol red (Gibco; powder stock), 1 g/L sodium bicarbonate ...
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bioRxiv - Microbiology 2024Quote: ... Plates were blocked with PBS-T containing 0.5% Omniblok non-fat milk powder (AmericanBio) and 3% goat serum (Gibco) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Heads were ground to a powder with a pestle and total RNA was extracted using Trizol reagent (Life Technologies) and Direct-zol RNA Microprep kit (R2062 ...
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bioRxiv - Microbiology 2024Quote: ... Plates were blocked with PBS-T containing 0.5% Omniblok non-fat milk powder (AmericanBio) and 3% goat serum (Gibco) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Whole tissue powder and mitochondrial isolations were lysed in RIPA buffer with protease/phosphatase inhibitor cocktail (ThermoFisher, Waltham, MA). Protein concentrations were determined by BCA (ThermoFisher) ...
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bioRxiv - Biochemistry 2020Quote: ... Single colonies of strains were inoculated into 2 mL of minimal medium containing 2% succinate and 50 µg/mL kanamycin in 14 mL polypropylene culture tubes (Fisher Scientific, Waltham, MA, USA), then grown to mid-exponential growth phase with shaking at 200 rpm and 30 °C on an Innova 2300 platform shaker ...
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bioRxiv - Immunology 2021Quote: ... Single colonies were subsequently isolated and cultured in TSB with 200ug/mL kanamycin in a shaking incubator (196rpm) at 37°C for 16 hours (MaxQ 4,450, Thermo Fisher Scientific, Canoga Park, CA). An additional two-hour subculture was performed in order to obtain bacteria in mid-logarithmic phase growth ...
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bioRxiv - Evolutionary Biology 2020Quote: The following types of microbeads were used: non-fluorescent 1 µm sulfate/latex beads (ThermoFisher Scientific S37498), non-fluorescent 2 µm sulfate/latex beads (ThermoFisher Scientific S37500) ...
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bioRxiv - Biophysics 2022Quote: ... The disks were decellularized in a 0.1% sodium dodecyl sulfate solution (SDS, Fisher Scientific, Fair Lawn, NJ) for 48h ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 µL of 2X loading buffer (1:2 diluted 4X lithium dodecyl sulfate sample buffer [Thermo Fisher NP0007] ...
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bioRxiv - Microbiology 2022Quote: Equivalent numbers of CHIKV particles were conjugated to 4-μm aldehyde/sulfate latex beads (Thermo Fisher Scientific) overnight at 4°C with gentle shaking ...
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bioRxiv - Microbiology 2022Quote: ... Nitrate and sulfate were measured by a Dionex ICS-5000 Ion Chromatography System (Thermo Scientific, CA, USA) equipped with an anion-exchange column (Dionex IonPac AS22 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... containing 100 units/ ml penicillin and 100 μg/ml streptomycin sulfate (Keygen Biotechnology) with 10% FBS (Gibco) under 37°C ...
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bioRxiv - Microbiology 2021Quote: ... 100 U/mL penicillin-G and 100 U/mL streptomycin sulfate (all purchased from Gibco/ThermoFisher, UK). Cells were maintained at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... 100 U/mL penicillin-G and 100 U/mL streptomycin sulfate (all purchased from Gibco/ThermoFisher, UK). Cells were maintained at 37 °C ...
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bioRxiv - Genetics 2020Quote: ... and antibiotics (100 units/ml penicillin G and 100 μg/ml streptomycin sulfate, ThermoFisher Scienticfic, Waltham, US.) at 37°C in a 5% CO2 incubator ...
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bioRxiv - Molecular Biology 2021Quote: ... PK was deactivated by addition of sodium dodecyl sulfate-containing NuPAGE LDS sample buffer (Thermo Fisher Scientific) and boiling of samples at 95°C for 5 minutes ...
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bioRxiv - Microbiology 2022Quote: Anti-ORF8 mAb (#1-3-2) was coupled to aldehyde/sulfate latex beads (Thermo Fisher Scientific A37304). The antibody was mixed with the latex beads and shaken at room temperature overnight ...
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bioRxiv - Zoology 2023Quote: ... Ten microliters of lithium dodecyl sulfate (LDS) 4X sample buffer (Bolt™, Thermo Fisher Scientific, Waltham, Massachusetts) was then added to the tube ...
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bioRxiv - Microbiology 2022Quote: ... COX8-EGFP-mCherry-expressing U2OS cells were then selected with 800 µg/ml of G418 sulfate (Gibco). An expression vector encoding for BNIP3 fused to mCherry (pmCherry C1-BNIP3 ...
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bioRxiv - Immunology 2024Quote: ... were resuspended in 200 μl of PBS and 10 µl of 4 µm aldehyde/sulfate latex (Invitrogen) beads were added per sample followed by a 15 min incubation at room temperature with rotation ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% sodium dodecyl sulfate and 5 mM ethylenediaminetetraacetic acid with protease and phosphatase inhibitors (Thermo Fisher Scientific). Cell lysates were centrifuged at 500 g for 30 minutes at 4°C to remove cellular debris ...
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bioRxiv - Biophysics 2023Quote: ... cells were maintained in selection media containing 800 μg/mL of G418 Sulfate (Thermo Fisher Scientific, 10131027) and 6 μg/mL of Blasticidin (Goldbio ...
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bioRxiv - Cell Biology 2023Quote: ... exosomes (10μg) isolated by differential centrifugation were coupled to 4μm surfactant-free aldehyde/sulfate latex beads (Invitrogen) and were incubated with primary antibody and fluorescently labeled secondary antibody and analysed by flow cytometry on BD FACScalibur (BD Bioscience) ...
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bioRxiv - Bioengineering 2023Quote: ... A 60.5 mM nitrogen concentration in the media was obtained with 4g/l ammonium sulfate (Acros Organics) or 1.82 g/l urea (Acros Organics) ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1% sodium dodecyl sulfate (SDS) and 5 mM EDTA with protease and phosphatase inhibitors (Thermo Fisher Scientific). Cell lysates were centrifuged at 500 g for 30 minutes at 4°C to remove cellular debris ...
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bioRxiv - Microbiology 2024Quote: ... Sulfate (SO42–) and calcium (Ca2+) concentrations were determined using a Dionex Ion Chromatograph (Thermo Scientific Dionex, USA). Dissolved metals (Fe2+ ...
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bioRxiv - Microbiology 2024Quote: ... The collected fractions were mixed with 2× Tris-glycine sodium dodecyl sulfate (SDS) sample buffer (Novex; Invitrogen) and subsequently subjected to SDS-polyacrylamide gel electrophoresis (PAGE ...
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bioRxiv - Neuroscience 2024Quote: ... cultured MPs were infected a mix of lentivirus and protamine sulfate (5 µg/mL, Thermo Fisher Scientific) to enhance lentiviral integration into the cells ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were stopped by adding LDS (lithium dodecyl sulfate) sample buffer (Invitrogen Life Technologies, Carlsbad, CA, USA) containing 2.5% v/v β-mercaptoethanol and boiling for 7 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were stopped by adding LDS (lithium dodecyl sulfate) sample buffer (Invitrogen Life Technologies, Carlsbad, CA, USA) containing 2.5% v/v β-mercaptoethanol and boiling for 7 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... was subjected to 4% to 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (cat no. NP032; Thermo Fisher), followed by transfer to polyvinylidene difluoride membrane (cat no ...
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bioRxiv - Cell Biology 2022Quote: ... The dry membrane was washed in blocking buffer (PBS + 4% milk powder + 0.1% Tween-20) for 20-30 min and subsequently incubated for 45 min in Streptavidin-HRP (Thermo Scientific) diluted 1:3000 in blocking buffer ...
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bioRxiv - Plant Biology 2020Quote: ... The snap-frozen tissue was then ground to a fine powder and RNAs were extracted using TRIzol® Reagent (ThermoFisher). cDNA synthesis was carried out using the Invitrogen M-MLV reverse transcriptase ...
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bioRxiv - Neuroscience 2024Quote: ... stock solution (8 mg/mL) was freshly prepared by dissolving RB powder in sterile saline (catalog number: NC9054335, Fisher Scientific) on the day of photothrombosis ...
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bioRxiv - Microbiology 2024Quote: ... Adjusted amounts of iRBCs were resuspended in binding medium (RPMI 1640 powder (Gibco 51800: W/L-Glutamine W/O NaHCO3) with HEPES pH 7 ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were blocked for 2hrs at RT in 5% milk powder and then incubated overnight (o/n) at 4°C with primary rabbit anti-GFP antibody (1:1,000; Molecular Probes). Membranes were washed 3x for 10min in 1X TBST ...
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bioRxiv - Biochemistry 2023Quote: ... RNA was extracted from ∼50 mg of muscle powder using the TRIzol Plus RNA Purification Kit (Invitrogen, cat. no. 12183555) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Blotted membranes were blocked with PBS containing 4% w/v skimmed milk powder followed by incubation with 1:1000 dilution of mouse anti-GFP antibody (GF28R, Invitrogen) in blocking buffer followed by incubation with 1:5000 dilution of goat anti-mouse IgG secondary antibody ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were extracted from tissues powder and the concentration was determined using the Pierce BCA assay kit (Thermo Fisher Scientific). 5 µg of total protein extracts were stacked on acrylamide-SDS gels.
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bioRxiv - Cell Biology 2023Quote: ... The cells were plated on 0.4% gelatin-coated 60 mm culture dishes (Gelatin Type B Powder, Sigma-Aldrich, G9391; ThermoFisher Scientific Cell Culture Petri Dishes ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 6-8 closed inflorescences ground to a fine powder by TRIzol™ (Thermo Fisher, #15596018) according to manufacturer’s instructions and precipitated in 1 volume of cold isopropanol ...