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Citations for Thermo Fisher :
601 - 650 of 1016 citations for Corning Kanamycin Sulfate Powder since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Protein gels were run using sodium dodecyl sulfate polyacrylamide gradient gels (NuPage 4−12% Bis-Tris gel from Invitrogen) using the BenchMark protein ladder (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: Porewater sulfate concentrations were measured in a Dionex ICS-5000 reagent-free ion chromatography system (Thermo Scientific, CA, USA) equipped with an anion-exchange column (Dionex IonPac AS22 ...
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bioRxiv - Biochemistry 2021Quote: ... Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) was performed on a NuPAGE Novex Bis-Tris gel following manufacturer’s instructions (Invitrogen). Protein/ACE2 or protein/mAb attached on magnetic beads or protein control in solution were mixed with 4X Sample Buffer and deionized water (1:3 v/v ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were eluted by incubating beads with 20 μl of 1X NuPAGE lithium dodecyl sulfate sample buffer (Invitrogen) at room temperature for 10 min ...
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bioRxiv - Immunology 2020Quote: ... cells were quickly lysed in 8% Lithium dodecyl sulfate LDS loading buffer preheated at 85°C (Invitrogen Cat#NP0007) and incubated with stirring at 500 rpm for 5 min at 85°C (Eppendorf ...
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bioRxiv - Genomics 2021Quote: ... Treated sections were then mounted with hybridization buffer (5%/15% Formamide; 10% Dextran sulfate Sigma D8906; 0.02% BSA Ambion AM2616 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then transferred to a 10cm dish and allowed to replicate for 14 days under selection at 750μg/ml Geneticin G418 Sulfate (Gibco). Cells were then stained with Halo-JF646 at 500nM for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were stopped by the addition of LDS (lithium dodecyl sulfate) sample buffer (Invitrogen Life Technologies, Carlsbad, CA, USA) containing 2.5% β-mercaptoethanol ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were stopped by the addition of LDS (lithium dodecyl sulfate) sample buffer (Invitrogen Life Technologies, Carlsbad, CA, USA) containing 2.5% β-mercaptoethanol ...
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bioRxiv - Cell Biology 2023Quote: ... and plaque-derived sEVs (n=6) were mixed with NuPAGE lithium dodecyl sulfate sample buffer (NP0007, Thermo Fisher Scientific) at a 4:1 ratio and NuPAGE sample reducing agent (NP0009 ...
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bioRxiv - Genetics 2023Quote: ... RKO and HT-1080 shRNA+ cells from the competition proliferative assay experiment were selected with Geneticin/G418 Sulfate (Gibco) for 7 days and checked for GFP expression ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein (between 10 and 20 ug) was resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE; ThermoFisher #NP0335) in 1X MOPS buffer (40 mM 3-(N-morpholino ...
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Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availabilitybioRxiv - Cell Biology 2023Quote: ... Polyacrylamide gel solutions were prepared by adding 99.4% v/v of polymer solution (of corresponding stiffness) with 0.5% v/v ammonium per-sulfate (ThermoFisher Scientific) and 0.1% v/v TEMED (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Bound S protein/ACE2 or S protein/mAb complexes were analyzed on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) on a NuPAGE Novex Bis-Tris gel following manufacturer’s instructions (Invitrogen). Protein solutions were mixed with 4× sample buffer and deionized water (1:3 v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 units/ml penicillin and 500 µg/ml of G418 (G418 Sulfate, 10131035, Thermo Fisher Scientific, Waltham, MA, USA) in a humified incubator at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were further cultured in the presence of 200-400 μg/ml Neomycin (G418 Sulfate, ThermoFisher Scientific, Cat# 11811023) to select for genetically modified cells ...
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bioRxiv - Synthetic Biology 2023Quote: ... Samples were placed in a 100 mL beaker with 40 mL of 1% sodium dodecyl sulfate (SDS) (Thermo Scientific) solution and agitated in an orbital shaker at 100 RPM at ambient temperature for 3 days ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we added filter sterilised magnesium sulfate (in each litre of medium, 500 μl of 10% w/v, Fisher Scientific) and thiamine hydrochloride (500 μl of 0.2% w/v ...
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bioRxiv - Neuroscience 2024Quote: ... Soluble protein samples were prepared with NuPAGE™ lithium dodecyl sulfate (LDS) and reducing agent (RA) (Thermo fisher Scientific) and boiled at 95°C for 15 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were standardized to equal concentrations and diluted into NuPAGE LDS (lithium dodecyl sulfate, pH 8.4) sample loading buffer (Invitrogen) prior to boiling for 10 minutes at 95°C ...
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bioRxiv - Molecular Biology 2022Quote: ... at which point they were diluted 100-fold (10-3 final dilution) in dilution buffer (1X PBS, 0.1% [w:v] sodium dodecyl sulfate, 1X N2 supplement [Life Technologies Corporation ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2 µg/ml of polybrene) for 24 h and selected with 0.5 mg/ml of Geneticin/G418 Sulfate (Gibco) for at least one week ...
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bioRxiv - Cell Biology 2023Quote: Colitis was induced in NOD SCID female mice by administration of 3.5% Dextran sulfate sodium salt (DSS, Affymetrix J14489) in drinking water for 7 days followed by normal water for 3 days ...
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bioRxiv - Cell Biology 2023Quote: ... for 5 min and hybridized with smFISH probes diluted 1:3000 in hybridization buffer (10% dextran sulfate, Sigma D8906, 20% formamide, 1 mg/ml E. coli tRNA, Sigma R1753, 2× SSC, 0.02% BSA, Ambion AM2616 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... samples were incubated in Tyramide development solution (2% Dextran sulfate, 0.0015% hydrogen peroxide, 0.2mg/ml Iodophenol, 1:100 Alexa Fluor 488 Tyramide (ThermoFisher # B40953) in PTw ...
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bioRxiv - Microbiology 2024Quote: Samples were processed and loaded on a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (4%–20% tris-glycine 1.0 mm Mini Protein Gels from Invitrogen). The gel was either stained with Coomassie blue and de-stained overnight prior to imaging ...
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bioRxiv - Neuroscience 2024Quote: ... Clones were selected from cultures grown for 14 days in the presence of 800 µg/ml G418 sulfate (Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... followed by Matrigel (Corning, Fisher Scientific). Then cells are placed under an inverted epifluorescence microscope (AxioObserver™ Z1 ...
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bioRxiv - Developmental Biology 2021Quote: Frozen placental samples were ground into a powder using a liquid nitrogen-cooled mortar and pestle then directly added to TRIzol reagent (Thermo Fisher #15596026); for cell lines ...
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bioRxiv - Genomics 2020Quote: Frozen placental samples were ground into a powder using liquid nitrogen-cooled mortar and pestle then directly added to TRIzol reagent (Thermo Fisher #15596026); for cell lines media was removed and TRIzol reagent was added directly to the tissue culture dish ...
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bioRxiv - Microbiology 2020Quote: ... frozen rumen-incubated biomass (switchgrass) was manually ground to powder in the presence of liquid nitrogen and immediately added to TRIzol reagent (Invitrogen, Carlsbad, CA). Next ...
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bioRxiv - Plant Biology 2024Quote: ... Contents of C and N were determined from 5 mg of tissue powder on a Flash 2000 Elemental Analyzer (Thermo Fisher Scientific), and contents of S were determined from 20 mg of tissue powder mixed with 5 mg of tungsten trioxide on an elemental PYRO cube analyzer (Elementar) ...
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bioRxiv - Physiology 2022Quote: GNM muscles of mice euthanized in the morning in fed state were ground in liquid nitrogen and proteins were extracted from the powder obtained using Mammalian protein extraction reagent (MPER, #78501, Thermo Fisher Scientific) supplemented with halt protease and halt phosphatase inhibitors (#78429 and #78426 ...
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bioRxiv - Genomics 2024Quote: ... distachyon plants were ground in liquid nitrogen and 500 μL of this powder was subjected to TRIzol extraction following the supplier instructions (Invitrogen, CA, USA). 20 µg of total RNA was treated with DNase I for 30 min. ...
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bioRxiv - Cancer Biology 2024Quote: ... Prior to RNA purification tissue was crushed under liquid Nitrogen and 25-50 mg of tissue-powder lysed in 800ml Ambion TRIzol Reagent (Invitrogen™,15596018) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... and 7.5 µg of lysate was run on a 4-12% Bis-Tris sodium dodecyl sulfate (SDS)-polyacrylamide gel with MOPS running buffer (Invitrogen) under reducing conditions ...
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bioRxiv - Cell Biology 2020Quote: ... the transfection mixture was replaced with growth media and were grown for an additional 48 hr before being replaced with growth media supplemented with 200 μg/ml G418 sulfate (Gibco).
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bioRxiv - Cancer Biology 2020Quote: 10U/ml penicillin G sodium and 10 mg/ml streptomycin sulfate (pen-strep; Gibco/ Invitrogen, Grand Island, NY; 15140-122).
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bioRxiv - Biochemistry 2020Quote: Purified rFIX samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using a 4-12% Bis-Tris Bolt acrylamide gel (Invitrogen) in 1x MES running buffer (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... Twenty μL of clarified lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 4-12% or 10% polyacrylamide Bis-Tris gels (Invitrogen) and transferred onto Immobilon FL PVDF or P PVDF membranes (Millipore ...
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bioRxiv - Cancer Biology 2020Quote: ... Geneticin™ Selective Antibiotic (G418 Sulfate) for selection of monoclonal lines expressing gene of interest was also purchased from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: Fluorescent polystyrene beads 0.2 μm in size (FluoSpheres sulfate microspheres F8848, carboxylate-modified microspheres F8811, amine-modified microsphere F8764; Thermo Fisher) were diluted 300 times to 0.02% (wt/vol ...
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bioRxiv - Cell Biology 2021Quote: ... Protein samples were loaded for each group in each well and run on 8-16% sodium dodecyl sulfate–polyacrylamide gel (ThermoFisher) for electrophoresis and then transferred to the PVDF membrane ...
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bioRxiv - Immunology 2022Quote: ... Proteins were separated by size on a 12% sodium dodecyl sulfate polyacrylamide gel and blotted onto a 0.2 µm PVDF membrane (Thermo Scientific) by semi-dry blotting (BioRad) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples of elution fractions were taken to perform sodium dodecyl sulfate polyacrylamide agarose gel electrophoresis (SDS-PAGE) (Bolt 4-12% Bis-Tris Plus, Invitrogen) at 180 V for 30 min in 1× MES buffer (Novex ...
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bioRxiv - Cancer Biology 2022Quote: ... and 0.06% Bromophenol Blue) at 98°C for 5 minutes and separated on 12% sodium dodecyl sulfate (SDS)-polyacrylamide gels (Invitrogen). Proteins were transferred to nitrocellulose membranes (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... RIPA extracts (50 µg of protein) were treated in NuPage lithium dodecyl sulfate (LDS) sample buffer (4X) (Thermo Fisher Scientific) supplemented with 50 mM dithiothreitol (DTT ...
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bioRxiv - Immunology 2020Quote: ... Samples containing 30 µg/µl of cell lysate proteins per lane were resolved on SDS-PAGE (10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis) along with pre-stained protein ladder and transferred onto PVDF membranes (Invitrogen). The transferred membranes were blocked for 2 hours using 5% BSA in TBST (25 mm Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: The purified proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 4-12% Bis-Tris NuPAGE gels (Invitrogen). Prior to SDS-PAGE analysis ...
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bioRxiv - Developmental Biology 2020Quote: ... Discs were transferred to hybridization buffer (10% w/v dextran sulfate, 4X SSC, 0.01% w/v salmon sperm ssDNA (Invitrogen 15632), 1% v/v vanadyl ribonucleoside (NEB S14025) ...