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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for 5 2 3 Difluorophenyl 5 oxovaleric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... were incubated for 20 minutes with 5 µM of Di-3-ANEPPDHQ (Thermo Fisher Scientific, USA) membrane dye ...
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bioRxiv - Neuroscience 2022Quote: ... washed 3 × 5 min with TBS and embedded in ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific). Images were taken with a Nikon Eclipse90i microscope and acquisition was performed with the NIS Elements software (Version 5.01) ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 nM (final concentration) shRNA or 3 ∼ 5 µg plasmid in 50 µL Opti-MEM (Gibco) was mixed with 5 µL transfection reagent (Lipofectamine ...
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bioRxiv - Microbiology 2022Quote: ... PV-3 SC8 VLPs or infectious PV-1 were incubated with 5 µM SYTO9 (Thermo Fisher) and SYPRO red (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... Data analysis was performed with the Quantstudio 3&5 Design and analysis software (Applied Biosystems, v1.5.1). Cq values were obtained via automated threshold determination ...
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bioRxiv - Neuroscience 2023Quote: ... PBS washes (3 × 5 min) and incubation with primary antibody (mouse anti-GFP, A11120, Life technologies; 1:200 ...
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bioRxiv - Microbiology 2024Quote: ... Amplifications were achieved using a 3-step program on a QuantStudio 5 system (Thermo Fisher Scientific). Transcript abundance was calculated using the ΔΔCT method (75 ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were subsequently washed 3 x 5 minutes in PBST 0.01% (DPBS ThermoFisher, Tween P1379 Sigma), permeabilised for 15 minutes in PBST 0.5% and washed in PBST 0.01% a further three times ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were then washed for 5 minutes 3 times in HistoClear (50-899-90147, Fisher Scientific) and briefly dried before the addition of Permount mounting media (SP15-100 ...
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bioRxiv - Cancer Biology 2024Quote: ... and were passaged in aggregates every 3-5 days using Versene solution (Thermo Fisher Scientific, #15040066) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: shRNAs targeting TRIM37 (TRIM37-1, 5’-tcgagaatatgatgctgtg-3’) were cloned into the pGIPz (Thermo Fisher Scientific) vector ...
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were split by incubating for 3-5 min at +37 L in EDTA (15575020, Invitrogen) and kept in 5 µM Y-27632 2HCl (S1049 ...
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bioRxiv - Developmental Biology 2024Quote: ... then blocked with blocking buffer (3% bovine serum albumin with 5% normal goat serum (Gibco, 16210064), and 5% normal donkey serum (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... n = 3) or oligopyridylamides (5 µM ADH-1 or ADH-6, n = 3) using a combination of both TriZol (Thermo Fisher Scientific) and RNAeasy Mini Kit (Qiagen ...
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bioRxiv - Plant Biology 2022Quote: 5’-biotinylated RNA and unmodified RNA of Motif 3 (5’-AAAUCGCCGGAG-3’ 5’-Bio-AAAUCGCCGGAG-3’) were synthesized by Eurofins (Hamburg, Germany) and used for LightShift™ Chemiluminescent RNA EMSA Kit (Thermo Scientific). Total protein was extracted from LL and 10 min LL➔HL-treated plants using extraction buffer (25 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... the hypervariable V3 region of the 16S rDNA gene was amplified from 20 ng of DNA using the primers 5′-CCTACGGGAGGCAGCAG-3′ and 5′-ATTACCGCGGCTGCTGG-3′ (Integrated DNA Technologies BVBA, Leuven, Belgium),(18) 1U Platinum® PCR SuperMix High Fidelity (ThermoFisher Scientific) and 10 μM of primer-mix ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated in 0.05 M Tris-HCl buffer (pH 7.6) containing 5 mg 3-3′ diaminobenzidine (Thermo Scientific, TA-060-HDX) per 10 mL buffer and a final concentration of 0.01% hydrogen peroxide for 15 min at RT ...
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bioRxiv - Immunology 2022Quote: ... and hybridized first using RT primer (5′/5Biosg/AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3′) and then reverse transcribed into cDNA using TSO primer (5′-AAGCAGTGGTATCAACGCAGAGTACATrGrGrG-3’) and RT maxima reverse transcription (Thermo Fisher Scientific). cDNA was amplified using ISPCR primer (5′-AAGCAGTGGTATCAACGCAGAGT-3′ ...
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bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
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bioRxiv - Plant Biology 2024Quote: ... The purified RNA was reverse transcribed using the CLIP-3 PCR primer (5’-GTCTCGTGGGCTCGG-3’) and SuperScript IV reverse transcriptase (18090010; Thermo Fisher Scientific). The first-strand cDNA was then subjected to PCR using the CLIP-5 PCR primer (5’-TCGTCGGGCAGCGTC-3’) ...
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bioRxiv - Microbiology 2024Quote: ... total RNA was harvested from a 10 ml parasite culture (5% hematocrit; >3% parasitaemia) by resuspending the pelleted RBCs in 3 ml pre-warmed Trizol (Invitrogen #15596-018). After a 5 min incubation step at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) (Gibco), Sodium Pyruvate (Gibco) ...
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bioRxiv - Biophysics 2021Quote: ... we labeled 5-30 μM protein with 2 mM DTNB (Ellman’s reagent, Thermo Scientific) in 20 mM Tris ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA (2-5 µg) was treated with DNAse I (Thermo Fisher Scientific, Cat # AM1906) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were incubated with 10 µM 5-ethynyl-2’ deoxyuridine (EdU, Thermo Fisher) for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were then blocked with 2% BSA and 5% donkey serum (ThermoFisher Cat # NC9624464) in PBS for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: Click-iT EdU (5-ethynyl-2’-deoxyuridine, a thymidine analog) kit from Life Technologies was used to perform DNA replication assay (Milton et al. ...
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bioRxiv - Genomics 2020Quote: ... 5 µl of 10X Turbo DNase buffer and 2 µl Turbo DNase (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... The samples were then reduced with 5 mM Tris(2-carboxyethyl)phosphine (ThermoFisher Scientific) for 30 minutes at 37 °C and then alkylated in the dark for 30 minutes at 25 °C with 40 mM iodoacetamide (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 5 μl of purified products were loaded on 2% E-Gel EX (Invitrogen) to verify the reaction performance and amplicon size ...
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bioRxiv - Neuroscience 2021Quote: ... and two injections of 5-ethynyl-2’-deoxyuridine (EdU: 0.01 mg/kg, Invitrogen #A10044), made as a 10 mM stock solution in DMSO and diluted for injection to 40% strength in 0.9% sterile saline ...
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bioRxiv - Developmental Biology 2022Quote: ... 5% mercapto-2-ethanol and 1X Halt Protease Inhibitor Cocktail (Thermo Scientific; 1:200). Proteins were separated using SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... and were supplemented with 10mM EdU (5-ethyl-2’-deoxyuridine) (ThermoFisher Scientific, Rockford, IL) during the final 6 hours of incubation to mark proliferating cells ...
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bioRxiv - Molecular Biology 2022Quote: ... mixed with 5 μl of 2 x Novex TBE-Urea sample buffer (ThermoFisher Scientific) and denatured for 3 minutes at 70°C ...
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bioRxiv - Molecular Biology 2022Quote: ... For labeling of proliferating cell populations with 5-ethynyl-2’-deoxyuridine (EdU) (ThermoFisher Scientific), animals received IP injections of EdU (50 mg/kg ...
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bioRxiv - Biophysics 2022Quote: ... 5 μM Fluo-2 Low Affinity (TefLabs) and 2.5 μM Alexa568 (Invitrogen, Waltham MA) as previously described (36) ...
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bioRxiv - Neuroscience 2022Quote: ... passed through Xylol (2 x 5 minutes) and immediately mounted with Eukitt® (ThermoFisher).
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bioRxiv - Developmental Biology 2020Quote: ... viride were incubated in 100 μM 5-ethynyl-2’-deoxyuridine (EdU; Thermo Fisher Scientific) diluted in ASW from 24 to 48 HPA ...
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bioRxiv - Microbiology 2021Quote: ... All medium were supplemented with 2-5 or 10% fetal bovine serum (FBS; Gibco) according to the experimental setting ...
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bioRxiv - Neuroscience 2020Quote: ... a ClickiT ® EdU (5-ethynl-2’-deoxyuridine) cell proliferation assay (Invitrogen, Paisley, UK) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated with 5-ehtynyl-2’-deox-yuridine (EdU: Invitrogen, Thermo Fisher Scientific) at 10 µM ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated with 5-ehtynyl-2’-deox-yuridine (EdU: Invitrogen, Thermo Fisher Scientific) at 10 µM ...
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bioRxiv - Molecular Biology 2021Quote: ... 2×10^5 individualized hESCs were resuspended in 10 μL Resuspension Buffer R (Invitrogen) containing CRISPR ribonucleoproteins (Cas9 protein+sgRNA ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM DTT and 5 U/mL SUPERase.In RNase Inhibitor(Thermo Fisher Scientific #AM2696)) on ice and post-nuclear extracts placed on top of 10-50% weight/volume sucrose gradients containing the same buffer (apart from no Triton X-100 ...
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bioRxiv - Cancer Biology 2021Quote: ... The target cells were treated with 5 mg/ml MMC (#BP2531-2, Fisher Scientific) for 90 minutes at 37°C and then washed twice with RPMI medium before adding to the T cells at a 2:1 ratio (CAR T:MMC treated cells) ...
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bioRxiv - Developmental Biology 2021Quote: Fish were incubated with 0.1 mg/ml of 5-ethynl-2-deoxyuridine (Thermo Fisher) in embryo media for 2 hours prior to harvesting at 98 hpf ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 hours and 5 hours using a Floid Cell Imaging Station (Thermo Fisher Scientist), and then manually counted for quantification ...
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bioRxiv - Evolutionary Biology 2023Quote: We performed experimental evolution using SM/5 media [56] (2 g glucose (Fisher Scientific), 2 g BactoPeptone (Oxoid) ...
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bioRxiv - Developmental Biology 2023Quote: Cell proliferation assays were conducted employing 5-Ethynyl-2′-deoxyuridine (EdU; ThermoFisher Cat# C10337), a thymidine analog ...
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bioRxiv - Cancer Biology 2022Quote: ... mice underwent intraperitoneal injection (IP) with 5-ethynyl-2`-deoxyuridine (EdU) (component A, Invitrogen™ Click-iT™ EdU Cell Proliferation Kit for Imaging ...