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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for 5 2 3 Difluorophenyl 5 oxovaleric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Nuclei were counterstained by Hoechst 33342 (Thermo Fisher Scientific, 2 μg/mL, 5 min, RT), and slides were mounted in ProLong Gold antifade mountant (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Reduction of proteins was achieved with 5 mM Tris(2-carboxyethyl)phosphine hydrochloride (Thermo Fisher) for 30 min and samples were subsequently alkylated in 10 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... Animals were injected intraperitoneally with 40 mg/kg EdU (5- ethynyl-2’-deoxyuridine; Invitrogen, E10187) before and after the session ...
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bioRxiv - Neuroscience 2022Quote: Mice were injected with 100 mg/kg of 5-ethynyl-2’-deoxyuridine (EdU; Invitrogen #E10187) by intraperitoneal injection for 5 consecutive days ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse IgG1 anti-alpha Tubulin Clone B-5-1-2 (1:500, ThermoFisher 32-2500), Rabbit anti-Myc polyclonal (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with a mix of 5 µM Fura-2 AM (Invitrogen™, F1221) and 0.025% Pluronic® F-127 (diluted in CM ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µL of DNase TURBO 10X Buffer and 2 µL of DNase TURBO (Invitrogen, #AM2238) were then added and the reactions are incubated at 37 °C for 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM Hepes-K+ pH 7.6) containing 2 mg/mL of collagenase type II (GIBCO) for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and 2-5 μl sample were loaded onto 10-20% SDS-tricine gels (Invitrogen, #EC66252BOX) and LPS was silver stained as described previously [47] ...
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bioRxiv - Biochemistry 2024Quote: ... Acclaim PepMap 100 nanoViper C18 (Thermo Scientific, 100 μm×2 cm, 5 μm, 100 Å) was used as a trap column ...
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bioRxiv - Biochemistry 2023Quote: ... Glycoproteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl; Thermo Scientific) and alkylated with 10 mM 2-Chloroacetamide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... Animals were injected intraperitoneally with 40 mg/kg EdU (5-ethynyl-2’-deoxyuridine; Invitrogen, E10187) before the session ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: ... Slides were counterstained with 5 μg/mL 4’,6-diamidino-2-phenylindole (DAPI; ThermoFisher Scientific) for 10 min at room temperature and washed with 1 × PBST (0.2% Triton-X 100 ...
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bioRxiv - Biochemistry 2023Quote: ... 2 μM purified DNMT3A2 was incubated in triplicate with 5× SYPRO Orange (Thermo Fisher Scientific) in assay buffer (50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... Recovered nuclei were stained with FITC-conujugated α-5-bromo-2’-deoxyurine (Invitrogen, MoBu-1) in staining buffer (2 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... 5-ethynyl-2’-deoxyuridine (EdU, 12.5 mg/kg, Thermo Fisher Scientific Inc. Waltham, MA, USA) was intraperitoneally injected into pregnant C57BL/6 mice once a day (12 P.M. ...
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bioRxiv - Developmental Biology 2023Quote: Tadpoles were immersed in a solution containing 1 mM EdU (5-ethynyl-2’-deoxyuridine; Invitrogen) before fixation ...
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bioRxiv - Neuroscience 2024Quote: ... then an IP injection of 50 mg/kg 5-ethynyl-2’-deoxyuridine (EdU (ThermoFisher Scientific)) 24 h later and were perfused after a further six days.
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bioRxiv - Molecular Biology 2024Quote: ... UHRR or HEK-293T RNAs (5 μg) were DNased with 2 U TURBO DNase (Invitrogen) and purified using an RNA Clean & Concentrator kit (Zymo Research ...
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bioRxiv - Neuroscience 2024Quote: ... Immunoprecipitation was performed with 5 µg of anti-BRN1/2 (PA5-30124 Thermo Fisher Scientific) or rabbit IgG as a negative control ...
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bioRxiv - Immunology 2024Quote: ... approximately 2-5×106 cells were incubated in 40 μl of fixable viability dye (Invitrogen) diluted in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Animals were injected intraperitoneally with 40 mg/kg EdU (5-ethynyl-2’-deoxyuridine; Invitrogen, E10187) before the session ...
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bioRxiv - Neuroscience 2024Quote: ... 12.5 mM Glucose and 10 μM 5’-fluoro-2’-deoxyuridine (FUDR; Thermo Fisher Scientific, 10144760), an antimitotic drug that suppresses growth of glial cells ...
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bioRxiv - Immunology 2024Quote: ... senescent CD4+ T cells were treated with BrdU (5-bromo-2′-deoxyuridine; 10µM; B23151-ThermoFisher) for 72 hours ...
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bioRxiv - Bioengineering 2022Quote: ... The 24-well plates with infected BHK-21 cells were overlaid with 1 ml of 0.8% methylcellulose in complete DMEM medium with 2% FBS and incubated for 5 days in a cell culture incubator (Thermo Fisher Scientific, 37°C and 5% CO2). Plaques were fixed and developed with staining reagent (1% crystal violet in 1:1 methanol/acetone solution ...
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bioRxiv - Molecular Biology 2023Quote: ... with IVT mix (0.5 µL Ribolock inhibitor, Invitrogen, 2 µL T7 polymerase buffer, NEB, 8 µL rNTP mix, 2 µL T7 polymerase, Invitrogen). DNA was removed by addition of 1 µL turbo DNase (Invitrogen ...
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bioRxiv - Biophysics 2024Quote: ... were seeded in a 6-well culture plate at a concentration of 2 x 105 cells ml-1 (2 ml per well in DMEM (Nissui) supplemented with 5 % fetal bovine serum (Gibco), glutamine ...
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bioRxiv - Immunology 2020Quote: ... BRET signal was read after 5 min of coelenterazine-h (5 μM, Invitrogen) addition ...
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bioRxiv - Cell Biology 2022Quote: ... Then 5 μg/ml Hoechst 33342 and 5 µM CellRox Deep Red (Invitrogen) and/or MitoSox Red (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: - Falcon round-bottom polystyrene tubes 5 mL (Cat# 14-959-5, Fisher Scientific)
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bioRxiv - Microbiology 2023Quote: ... 5-fluorocytosine (100 mg/kg/d; diluted in sterile saline; 5-FC; ThermoFisher) was used in combination with PEA (0.5 mg/kg/d ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 5% fetal calf serum and 5% newborn calf serum (both GIBCO) and antibiotics (#A5955 ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 5% fetal calf serum and 5% newborn calf serum (both GIBCO), and antibiotics (#A5955 ...
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bioRxiv - Microbiology 2024Quote: ... 0.6 μL of 5 μM TaqMan probe (5’-FAM-CAAGAGGTGGACGGCC-MGB) (ThermoFisher Scientific) and 0.1 μL of nuclease free water ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μm 0.3 x 5 mm nano trap column (Thermo Fisher Scientific,UK) and an Aurora ultimate TS 75 μm x 25 cm x 1.7 μm C18 column (IonOpticks) ...
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bioRxiv - Molecular Biology 2022Quote: ... pLV-mCherry at ratio 1:1:1 (i.e. 5:5:5 μg) using Lipofectamin™ 3000 transfection reagent (Thermo-Fisher Scientific, #L3000015). Pseudoviruses harvested from the supernatant at 48 h 72h post-transfection were filtered (0.44 μm ...
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bioRxiv - Cell Biology 2023Quote: ... Leukocytes were washed with cold PBS followed by another centrifugation step (300 x g 5 minutes) and resuspended in 5 mL of 5 μg mL-1 of Hoechst 33342 (Thermo Fisher Scientific) diluted in warm PBS ...
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bioRxiv - Pathology 2023Quote: ... one lung section 5×5×5 mm in size was finely cut and stored at -20°C in RNAlater (Thermo Scientific, US). We mechanically disrupted the tissue from RNAlater using a MediMachine (BD Biosciences ...
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bioRxiv - Biochemistry 2024Quote: ... the RNA was subjected to 5’ adapter ligation with a 5’ chimeric DNA-RNA adapter (5’aminolinker-GTTCAGAGTTCTACAGTCCGACGATCrNrNrNrN) using RNA ligase (EL0021, Thermo Fisher Scientific) at 37°C for 1 hour ...
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bioRxiv - Genomics 2020Quote: ... Genomic PCR products obtained with primers 5’-CGCCATCAACTTCACCTAGC-3’ (forward) and 5’-TCTGGGAAGAAGTTTGGCCT-3’ (reverse) were sequenced using the BigDye® Terminator v1.1 Cycle Sequencing Kit (Thermo Fisher Scientific, Massachusetts, USA) on an ABI 3130xl Genetic Analyzer (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... a probe prepared by PCR on genomic mouse DNA with the primers 5’-CATATTCCAGGTCCTTCAGTGTGC-3’ and 5’-CACTTTAGGACGTGAAAT ATGGCG-3’ was labeled with Cy3 by random priming according to the kit instruction (Invitrogen Kit, Ref 18095–011).
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bioRxiv - Bioengineering 2021Quote: ... sgRNA LA93527 was generated from a PCR DNA template (overlapping primers LA935 5’-GAAATTAATACGACTCACTATAGGACAGTGCGGTCCG-CAAGGGTTTTAGAGCTAGAAA-3’ and LA137 5’-AAAAGCACCGACTCGGTGCCA-CTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC-3’) containing the T7 promoter using the MEGAscript T7 Transcription kit (ThermoFisher Scientific, Walthum, MA USA). The reaction mix was incubated at 37°C for 16 hours and purified using the MEGAclear Transcription Clean-Up kit (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 5’TYE563-labeled locked nucleic acid probes (Exiqon) against mouse major satellite sequences (Supplementary Table 5) were precipitated with mouse Cot-1 DNA (Invitrogen) and Salmon Sperm DNA (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... and under a 12-hour day/night photoperiod schedule (Percival Scientific, IA, USA) with 5% dextrose (w/v) and 0.05% para-Aminobenzoic acid (w/v) (Thermo Fisher). Mosquito oocysts were checked at 10-days post-infection ...
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bioRxiv - Neuroscience 2020Quote: ... MEF were grown as monolayers at 37°C with 5% CO2 in DMEM supplemented with 10% serum and MEM non-essential amino acids (Gibco), or with 5% delipidated fetal bovine serum and 35 μM oleic acid ...
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bioRxiv - Biochemistry 2022Quote: ... the gel was rinsed in Milli-Q water for 5 minutes and stained for 20 minutes with 1x SYBR gold nucleic acid stain (Invitrogen) in 1x TBE ...
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bioRxiv - Genetics 2022Quote: ... as well as fibroblasts and SH-SY5Y cells treated for 5 hours with 100 µM oleic acid (OA) following over-night starvation in Opti-MEM (ThermoFisher). OA is a potent inducer of lipid droplet formation (55) ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: Total RNAs of patients were extracted from either FFPE (Formalin-fixed paraffin-embedded) or frozen sections (5 x 20µ) using RecoverAll Total Nucleic Acid Isolation (Ambion) or Trizol (Ambion ...
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bioRxiv - Pathology 2020Quote: ... citric acid (Fisher Scientific, Cat. No. A104-3, Hampton, NH), sodium phosphate (Sigma Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... 3 sodium pyruvate and 0.40 L-ascorbic acid (Acros Organics), bubbled to a pH of 7.4 with 5% CO2 in 95% O2 ...