Labshake search
Citations for Thermo Fisher :
7851 - 7900 of 10000+ citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2021Quote: ... These two proteins were labeled at the cysteine using thiol-reactive BODIPY™ FL N-(2-Aminoethyl) Maleimide (Thermo Fisher). The proteins were mixed with 10-fold excess BODIPY™ FL N-(2-Aminoethyl ...
-
bioRxiv - Microbiology 2020Quote: ... The plasmids were purified from 2 ml of the O/N culture using the GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific), and sent for Sanger sequencing to DNA Sequencing and Genomics ...
-
bioRxiv - Microbiology 2020Quote: PvRBP2b161-1454 and TfR1 were labelled with N-hydroxysuccinimide ester-activated DyLight 488 (DL488) and DyLight 594 (DL594) (Life Technologies) respectively ...
-
bioRxiv - Cell Biology 2021Quote: ... M2DCTs with an MyBP-C antibody (N-terminal rabbit polyclonal, 1:1000, kind gift from Samantha Harris) and anti-rabbit AlexaFluor secondary (ThermoFisher). Intercalated disk images were obtained by labeling fixed M2DCTs with an N-cadherin antibody (1:200 ...
-
bioRxiv - Cell Biology 2021Quote: ... C-term (aa 166-499)) were produced by PCR from the HA-PRPF31 plasmid and cloned into the pcDNA3.1 N-HA vector (Thermo Fisher). Deletion constructs of PRPF6 (NTD (aa 1-126) ...
-
bioRxiv - Biophysics 2022Quote: ... Purified Syb2 (29–96, S61C) was labeled with 6× molar excess BODIPY FL N-(2-aminoethyl)-maleimide (BDPY) (Molecular Probes) and SN25 FL (1–206 ...
-
bioRxiv - Biophysics 2022Quote: The SARS-CoV-2 Omicron RBD and the N-terminal peptidase domain of ACE2 were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as previously stated ...
-
bioRxiv - Microbiology 2022Quote: ... The blocking buffer was then aspirated and 50 μL of a 1:4000 dilution of rabbit anti-SARS-CoV-2 nucleocapsid (N) antibody (MA536086, Invitrogen) in blocking buffer was added and incubated for 2 h at 37°C ...
-
bioRxiv - Microbiology 2022Quote: SARS-CoV-2 Beta RBD for crystallization (residues 328-531 of S protein from GenBank NC_045512.2 with N-terminal signal peptide and C-terminal 8xHis-tag) was expressed in Expi293F (Thermo Fisher Scientific) cells in the presence of 10 µM kifunensine at 37°C and 8% CO2 ...
-
bioRxiv - Neuroscience 2022Quote: ... The real-time quantitative PCR (qRT-PCR) was performed with TaqMan Fast Advanced Master mix (ThermoFisher Scientific, catalog n°4444963) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... a 6 x His tag and linker was fused to the N-terminus of the GJA1-20k sequence and cloned into the pET301/CT-DEST vector via Gateway cloning (Invitrogen). The primers used to amplify the sequence are (5’ to 3’ ...
-
bioRxiv - Neuroscience 2022Quote: ... was dissolved in 0.9% sterile Saline and pH was adjusted to 7.4 with 20-30 µl 1 N NaOH (#SS266-1, Fisher Scientific) according to Tocris Bioscience’s guidelines ...
-
bioRxiv - Developmental Biology 2022Quote: Ear or tail biopsies were lysed overnight (o/n) at 55°C in DirectPCR lysis reagent (BioSite) in the presence of 0.1 mg/mL Proteinase K (Thermo Scientific). Lysis was stopped by heat inactivation (45min ...
-
bioRxiv - Neuroscience 2020Quote: ... and equally distributed in wells containing growth medium (DMEM-F12-glutamax [Gibco], 40 μg/mL insulin [Sigma], 1/200 B-27 supplement [Gibco], 1/100 N-2 supplement [Gibco] ...
-
bioRxiv - Microbiology 2019Quote: Immunohistochemistry was performed using monoclonal antibodies directed to MV N protein (clone 83KKII, Chemicon [57]) or rabbit polyclonal antibody directed to GFP (Invitrogen). Goat anti-mouse IgG1 or goat anti-rabbit antibody conjugated with biotin was included as secondary antibody ...
-
bioRxiv - Immunology 2020Quote: ... and SARS-CoV-2 and the N-terminal peptidase domain of human ACE2 (residues Ser19-Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as previously described 18,19,37-39 ...
-
bioRxiv - Microbiology 2020Quote: Human IFITM proteins and SARS-CoV-2 viral proteins were cloned into MaMTH N-term tagged Prey and C-term tagged Bait vectors respectively using Gateway cloning technology (ThermoFisher). Correctness of recombined insertions was confirmed by Sanger sequencing (Eurofins) ...
-
bioRxiv - Microbiology 2020Quote: Beads were labeled with the following N-hydroxysuccinimide ester (NHS)-activated fluorophores: (i) Alexa Fluor 488 NHS ester (Fisher Scientific) (ii ...
-
bioRxiv - Microbiology 2021Quote: ... and the N-terminal peptidase domain of human ACE2 (residues Ser19–Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as described previously21 ...
-
bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 N standards were generated by in-vitro transcription of the generated SARS-CoV-2 N cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by using the MEGAclear Transcription Clean-Up Kit ...
-
bioRxiv - Cancer Biology 2021Quote: ... NPCs were plated on Geltrex in a 6 well plate in StemPro NSC SFM supplemented with 20ng/ml of EGF and bFGF for 2 days before switching to astrocyte differentiation medium consisting of DMEM with 1% N-2 supplement (Gibco), 1% GlutaMAX-I supplement (Gibco ...
-
bioRxiv - Immunology 2021Quote: ... IF staining was performed on deparaffinized and rehydrated tissue sections using rabbit anti-SARS-CoV-2-N protein antibody together with AF568-conjugated goat anti-rabbit IgG (Invitrogen). Briefly ...
-
bioRxiv - Bioengineering 2021Quote: ... The ovaries were dissected into 2–4 pieces and transferred into maintenance media (α-MEM, P/N 32561, Gibco, USA) and placed in a CO2 incubator prior to encapsulation.
-
bioRxiv - Neuroscience 2022Quote: Freshly prepared solubilized proteins were incubated o/n at 4°C with affinity-purified rabbit anti-P2X4 antibodies (Alomone) cross-linked to magnetic beads (Dynabeads, Invitrogen). The flow through was discarded and the beads washed 5 times with wash buffer (CL48 diluted ¼ in PBS and supplemented with complete protease inhibitor cocktail (Roche) ...
-
bioRxiv - Neuroscience 2022Quote: ... Detection and relative quantification of the prevalence of individual N-glycans was accomplished using the total ion mapping (TIM) functionality of the Xcalibur software package version 2.0 (ThermoFisher Scientific) as previously described (Aoki et al. ...
-
bioRxiv - Microbiology 2022Quote: ... which were subsequently purified and ligated upstream the His-tag into the expression vector pTrcHis2 (Invitrogen™, cat. n° V36520). The recombinant vectors were transformed into the ΔarsC E ...
-
bioRxiv - Cell Biology 2019Quote: The pellets (n = 5/time point/group) were rinsed in PBS and lysed in 500 μl TRIzol (Invitrogen, CA, USA) for 10 min ...
-
bioRxiv - Microbiology 2019Quote: ... prepared minipreps from 2 ml of O/N cultivation at 37 °C using the GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific), checked for the presence of insert using restriction digest and agarose gel electrophoresis ...
-
bioRxiv - Biophysics 2019Quote: ... the HEK-293T cells were transfected with a plasmid encoding full-length LRRK2(I2020T) fused with YFP at the N-terminus using a Lipofectamine 3000 transfection kit (Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2020Quote: The C-terminal fragments of Stu2 used in the pulldown experiments were cloned into a pLIC vectors with a TEV-cleavable N-terminal 6-His tag and transformed into Bl-21 AI cells (Invitrogen). Cells were grown to an OD600 of 0.6 in TB media with 0.1% glucose and were induced by addition of IPTG and arabinose to 200 μM and 0.2% ...
-
bioRxiv - Cell Biology 2021Quote: ... WT human PRRT2 (gene ID: 112476) was synthesized as N-terminal HA tag fusion protein (Genewiz) in pcDNA3 vector (Thermofisher) and single point mutants were generated on this matrix by standard molecular biology techniques.
-
bioRxiv - Biochemistry 2021Quote: ... Julien Prudent (MRC Mitochondrial Biology Unit, Cambridge, UK) and grown in Dulbecco’s Modified Eagle’s Medium (Life Technologies, cat. n. 31885023) supplemented with 10% FBS (Life Technologies ...
-
bioRxiv - Developmental Biology 2020Quote: ... the insert from the entry clone was transferred to the destination clone Vivid Colors pcDNA 6.2/N-EmGFP-DEST (Life Technologies) via a Gateway LR Clonase reaction to produce the following plasmids ...
-
bioRxiv - Microbiology 2020Quote: ... and posteriorly cleaved with BamHI/PstI restriction enzymes and inserted in-frame to an N-terminal polyhistidine tag in the expression vector pRSETA (Invitrogen), previously digested with the same enzymes ...
-
bioRxiv - Microbiology 2020Quote: ... in vitro transcription of the ligated cDNA (along with a N-gene containing cDNA) using an mMachine T7 transcription kit (Ambion), and subsequent electroporation into BHK-R cells ...
-
bioRxiv - Microbiology 2020Quote: ... The reaction was stopped by addition of 0.5 N HCl and absorbance at 450 nm was measured using a Muktiskan FC microplate photometer (Thermo Scientific).
-
bioRxiv - Biochemistry 2021Quote: ... The resulting PCR product (N linker::CeTurboID::c-Myc::mNeongreen::3XFLAG) was cloned into position 2 of a Multisite Gateway pDONR vector (ThermoFisher).
-
bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 N standards were generated by in-vitro transcription of the generated SARS-CoV-2 N cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by using the MEGAclear Transcription Clean-Up Kit ...
-
bioRxiv - Neuroscience 2019Quote: ... 150 ng of total RNA extracted from the brainstem of Vglut2:Ndufs4cKO (n=4) and Vglut2:Ndufs4cCT mice (n=4) was amplified and biotin-labeled using the Illumina TotalPrep RNA Amplification kit (Ambion). 750 ng of the labeled cRNA was hybridized to MouseRef-8 v2 expression beadchips (Illumina ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... We subsequently placed two frozen individuals from each family and treatment group (n = 9 families * 2 treatments (+/− supplemental thiamine) * 2 individuals = 36) into 10 volumes of RNAlater-ICE (Invitrogen) pre-chilled to −80 °C and allowed the samples to reach −20 °C overnight ...
-
bioRxiv - Immunology 2021Quote: Codon-optimized sequences for the N or the spike S protein of SARS-CoV-2 were cloned into the pVAX1 expression plasmid (ThermoFisher) optimized for DNA vaccinations referred to as pVAX1-SARS2-N and pVAX1-SARS2-S 81 ...
-
bioRxiv - Cell Biology 2021Quote: ... and recombined with an RPS5A::XVE promoter and an N-terminal GFP in a pB7m34GW backbone (Karimi et al., 2007) via an LR reaction (Invitrogen) and verified by restriction digest.
-
bioRxiv - Physiology 2020Quote: ... and incubated with 100 mM 6-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (6-NBDG) (Life Technologies) in 10 nM Tris/HEPES buffer containing 150 mM KCl or 150 mM NaCl for 30 minutes at 37 °C ...
-
bioRxiv - Molecular Biology 2020Quote: Plasmid encoding the N-terminally His-tagged Msm HelD protein was prepared by the GeneArt® Plasmid Construction Service (Thermofisher). Gene construct for HelD expression was designed by codon-optimized back translation of gene MSMEG_2174 from Msm (strain ATCC 700084 / mc2 155 ...
-
bioRxiv - Molecular Biology 2019Quote: ... 6X N-terminally Myc-tagged cDNAs encoding Ku70 or Ku80 WT and mutants were cloned into pcDNA/FRT/TO (Invitrogen) and then transfected along with Flp recombinase expressing pOG44 plasmid using Lipofectamine transfection reagent (Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: ... was crosslinked to the PAAm gels using N-sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino)hexanoate (sulfo-SANPAH, from Life Technologies). 30 μL of 1mg/mL sulfo-SANPAH dissolved in milliQ H2O was added to PAAm gels which were irradiated under a 365nm UV lamp (ABM ...
-
bioRxiv - Biochemistry 2021Quote: DNA sequences encoding N-terminally GFP-fused and truncated FAM98B constructs were cloned into the pcDNA5/FRT/TO/nHASt vector (Invitrogen). The resulting FAM98B truncations contained an N-terminal HA-(StrepII)2-GFP tag ...
-
bioRxiv - Immunology 2021Quote: ... cells were incubated in glucose-free media containing 5 μg/ml 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher) and 2.5% FBS at 37 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... total RNA from testes (n = 4 males) was extracted by using the commercial RNAqueous®-Micro kit (Ambion, Austin, USA), according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... both human primary myogenic progenitors isolated from female donors (n=2-5 per experiment) and commercialised human primary skeletal muscle progenitors (ThermoFisher Scientific ...