Labshake search
Citations for Thermo Fisher :
7801 - 7850 of 10000+ citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... larvae were immersed in 3 µM FM 1-43FX (ThermoFisher, F25255) in E3 for 30 s ...
-
bioRxiv - Cancer Biology 2024Quote: ... along with gene specific primers using a QuantStudio 3 (Applied Biosystems). Primers used are listed in Supplemental Table 1.
-
bioRxiv - Cancer Biology 2024Quote: ... Subsequent qPCR was performed on a Quant Studio 3 instrument (ThermoFisher), utilizing gene-specific PCR primers mixed with Power SYBR Green Master Mix (ThermoFisher ...
-
bioRxiv - Neuroscience 2024Quote: ... Real-time qPCR was performed using QuantStudio 3 instruments (Applied Biosystems). The mixture for qPCR reaction was prepared in a final volume of 20 μl containing 1 μl cDNAs and 10 μl of LightCycler 480 SYBR Green I Master (Roche ...
-
bioRxiv - Biochemistry 2024Quote: ... and 3) Dulbeccòs phosphate buffer saline pH 7.4 (Gibco 14040-133). All substrates were tested at 20 µM ...
-
bioRxiv - Bioengineering 2024Quote: ... cells (3 ×105 cells total) were washed with PBS (1X, Gibco), pelleted at 180 xg ...
-
bioRxiv - Microbiology 2024Quote: ... falciparum parasites were cultured at 3% hematocrit in RPMI 1640 (Gibco) medium consisting of 0.5% Albumax II (Gibco) ...
-
bioRxiv - Microbiology 2024Quote: ... and an InvitrogenTM QubitTM 3 Fluorometer (Thermo Fisher Scientific Inc., USA) were used to confirm the quality and concentration of the extracted DNA ...
-
bioRxiv - Microbiology 2024Quote: ... the bacteria were stained with TO-PRO®-3 (ThermoFisher Scientific) to distinguish them from fecal debris or particles ...
-
bioRxiv - Bioengineering 2024Quote: ... we neutralized acid-extracted Collagen I (3 mg/ml, Gibco, A1048301) using 10X DMEM and 2N NaOH ...
-
bioRxiv - Biochemistry 2024Quote: ... 3 µM POLDIP2 and/or 10 µM hemin (Fisher Scientific AAA1116503) were included as indicated ...
-
bioRxiv - Developmental Biology 2024Quote: ... Texas-red Dextran (3 kDa and 70 kDa from Thermo Fisher) and AF647 conjugated α-bungarotoxin protein (aBt ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... or the QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific) (Z ...
-
bioRxiv - Physiology 2024Quote: ... Signal intensity was captured using QuantStudio 3 qPCR system (Applied Biosystems) under the following conditions ...
-
bioRxiv - Neuroscience 2024Quote: ... the cells were washed 3 times with sterile PBS (Gibco, 10010023) and then transferred to a 96-well v-bottom plate and centrifuged at 300g for 5 minutes at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... Data analysis was performed using Compound Discoverer 3 (Thermo Fisher Scientific) following standard procedures ...
-
bioRxiv - Cell Biology 2024Quote: ... Proteins were separated on 3-8% Tris-Acetate gels (Invitrogen #EA0375) using Tris-Acetate SDS running buffer (Invitrogen #LA0041) ...
-
bioRxiv - Developmental Biology 2024Quote: MEX-3 was fluorescently labeled with DyLight-488 NHS Ester (ThermoFisher) (Putnam and Seydoux ...
-
bioRxiv - Developmental Biology 2024Quote: ... 2 and 3 using the EVOS XL core microscope (Life Technologies).
-
bioRxiv - Plant Biology 2024Quote: qPCR was performed using QuantStudio 3 Real-Time PCR system (ThermoFisher) with PowerUp SYBR Green Master Mix (ThermoFisher) ...
-
bioRxiv - Microbiology 2020Quote: ... the sections were sequentially incubated with mouse polyclonal antibody to SARS-CoV-2 N protein (1:500 dilution) and HRP-conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The sections were observed under microscope (Olympus ...
-
bioRxiv - Cancer Biology 2021Quote: The Asparagine to Glutamine (N-Q) point mutation was performed using a Phusion SDM kit (Thermo Fisher Scientific, CAT# F541). In this assay ...
-
bioRxiv - Cell Biology 2020Quote: ... The Flp-In T-Rex HEK293 cells were transfected with the N-terminal GFP-tagged FAM83F packaged in a pcDNA5-FRT/TO vector along with Flp recombinase pOG44 (Invitrogen) in a ratio of 1 μg ...
-
bioRxiv - Developmental Biology 2021Quote: The Emerald Green Fluorescent Protein (EmGFP) -encoding region from Vivid ColorsTM cDNA pcDNATM6.2/N-EmGFP-DEST Vector (Thermo Fisher Scientific) was placed upstream of the coding sequence of PmlVI (human PML transcript variant 5 ...
-
bioRxiv - Genetics 2021Quote: ... https://www.lifetein.com) polyclonal antisera raised against the N-terminal peptide of CenH3 [66] and anti-Rabbit-Alexa Fluor 488 (ThermoFisher Scientific). KNL2 and CENP-C were detected in root nuclei as previously described [67] using custom made anti-AtKNL2 [37] or anti-AtCENP-C antibodies (http://www.eurogentec.com/ ...
-
bioRxiv - Bioengineering 2020Quote: ... Tipless MLCT-D probes (0.03 N/m spring constant) were functionalized with 10 μm diameter borosilicate glass beads (Thermo Scientific 9010 ...
-
bioRxiv - Developmental Biology 2021Quote: Lymph node divested mammary glands (excluding the cervical pair) were dissected from the mice and digested O/N in DMEM/F12 (Gibco) + 10 mM HEPES (Gibco ...
-
bioRxiv - Developmental Biology 2021Quote: Total RNA was isolated from the 60 hpa tissues (n=6) from each group using TRIzol reagent (Applied Biosystems, USA). One microgram of total RNA was reverse-transcribed to cDNA using a one-step cDNA Synthesis Kit (Applied Biosystems ...
-
bioRxiv - Neuroscience 2022Quote: Genes encoding the full-length sequences of Human PP2A A subunit and PP2A C subunit with N-terminal His tag and non-cleavable HA tag were sub-cloned into the pFastBac-Dual vector (Invitrogen). Sequences of PP2A A and C subunits are shown in Table 1 ...
-
bioRxiv - Plant Biology 2022Quote: ... AtSUMO1 was cloned into destination vector pK7WGR2 (N-terminal mRFP tag, (Karimi et al., 2002)) by LR reactions using Gateway technology (Invitrogen). Vector specific primer p35s-F used in plasmid construction are listed in Table S2.
-
bioRxiv - Physiology 2022Quote: ... at 1-1.5 x 106 cells per well in William’s medium (catalog n°12551032, Gibco, Thermofisher Scientific, Waltham, MA, USA) supplemented with 5% FBS ...
-
bioRxiv - Biochemistry 2022Quote: ... and 0.3 mM ATP were reacted with a 10-fold molar excess of N-(1-pyrene)iodoacetamide (Thermo Fisher Scientific) dissolved in dimethylformamide (DMF ...
-
bioRxiv - Molecular Biology 2020Quote: ... of “QTARKSTGGKAPRK” related peptides (1xAcetyl [K10]; 1xGln->pyro-Glu [N-Term]; 1xmethylations [K5]) using Qual Browser (Thermo xcalibur 4.1.50, Thermo Fisher Scientific) with ±15ppm width.
-
bioRxiv - Molecular Biology 2020Quote: ... at 200 V for 40 min in NuPAGE 2-(N-morpholino)ethanesulfonic acid (MES) running buffer (cat. no. NP0002, Invitrogen), supplemented with NuPAGE antioxidant (cat ...
-
bioRxiv - Immunology 2021Quote: ... Glucose uptake was measured by the uptake of glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG, Life Technologies). Cell surface and intracellular cytokine stainings of splenocytes and blood lymphocytes were performed as described (42) ...
-
bioRxiv - Genomics 2020Quote: ... a puromycin N-acetyltransferase and the WPRE next to stuffer sequences was ordered as a GeneArt Synthetic Gene (Life Technologies) and PCR-amplified ...
-
bioRxiv - Biochemistry 2019Quote: ... pET151/D-TOPO plasmids with the sequences of interest inserted under control of Lac operon and T7 viral promoter with N-terminal hexa-histidine tag and Ampicillin selection marker was obtained from Invitrogen. Sequences were optimized for expression in E ...
-
bioRxiv - Molecular Biology 2019Quote: ... The sequence of RVFV IGR and flanking nucleotides of the N and NSs genes were synthesized by GeneArt (Thermo Fisher) for stepwise assembly of the complete minigenome in the pX12ΔT vector ...
-
bioRxiv - Biophysics 2020Quote: A full-length cDNA encoding the methyltransferase SUV39H1 was amplified by PCR and fused with an N-terminal His6-MBP tag in pET-Duet1 (Invitrogen).
-
bioRxiv - Molecular Biology 2019Quote: ... were used for studies of INS-1 cells treated with CSE or AC extract with or without 5 mM N-Acetyl Cysteine (NAC) from Invitrogen; Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2019Quote: ... a Tet-inducible EGFP-RBM45 construct was generated by inserting the EGFP-RBM45 (N-terminal EGFP fusion) into the pcDNA4/TO vector (Invitrogen). The sequences of all constructs were confirmed by Sanger DNA sequencing and the sizes of the expressed proteins confirmed by Western blot.
-
bioRxiv - Molecular Biology 2019Quote: ... The VTN-N solution was removed prior to sticking the Press-to-Seal Silicone Isolator with Adhesive (Thermo Fisher Scientific) onto the nanosheets to control the density of the cells (see “Cell cultures” in METHODS).
-
bioRxiv - Immunology 2021Quote: ... virus-infected cells were detected using an in-house rabbit polyclonal antibody specific for SARS-CoV-2 N/M/E followed by horseradish peroxidase-conjugated goat anti-mouse IgG (Invitrogen). TCID50 was calculated using the Reed-Muench method.
-
bioRxiv - Biochemistry 2021Quote: ... A673 (ATCC-CRL-1598) and SK-N-MC (ATCC-HTB-10) cells were grown in Dulbecco’s modified Eagle Medium (DMEM, Thermo Fisher) supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Bioengineering 2020Quote: ... A biphasic solvent overlay was employed to capture patchoulol by adding 7 mL of filter-sterilized n-dodecane (Acros Organics) to each culture (10% v/v) ...
-
bioRxiv - Cell Biology 2021Quote: ... full length N-terminally 3xFLAG tagged human KNSTRN ORF was cloned into pTREx-DEST30 vector by serial Gateway recombinations (Invitrogen) according to the manual ...
-
bioRxiv - Cell Biology 2021Quote: ... TDP-43 CRISPR KO HeLa cells were lysed for N/C fractionation and SDS-PAGE with the NE-PER kit (ThermoFisher) according to the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2020Quote: ... the mouse mNCK2 cDNA sequence was cloned via restriction enzymes in frame with a N-terminal 3XFLAG tag in pcDNA3.1(-) (ThermoFisher Scientific) for western blot (WB ...
-
bioRxiv - Biochemistry 2020Quote: ... were PCR-amplified and cloned into the pDEST15 vector encoding an N-terminus GST tag using the Gateway Cloning Technology (Invitrogen)27 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... the protein with an N-terminal His10 tag was expressed in Sf9 cells following the Bac-to-Bac baculovirus system (Invitrogen). Cells were collected and solubilized to extract protein in the buffer containing 25 mM MES pH 6.0 ...