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Citations for Thermo Fisher :
7901 - 7950 of 10000+ citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... PC12 cells were also transiently transfected with KOR fused to FLAG on its n terminus using Lipofectamine 2000 transfection kit (ThermoFisher). Transfected cells were imaged two-three days after transfection ...
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bioRxiv - Cell Biology 2019Quote: ... Stably transfected HEK293 cells expressing N-terminally FLAG-tagged MOR or KOR were cultured in the presence of 250 μg/ml Geneticin (Gibco). For transient DNA expression ...
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bioRxiv - Biophysics 2021Quote: ... norvegicus with a HA-tag added to the C-terminal end representing the N-terminal domain (Met 1 to Val 469) of sAC (57) was cloned into pcDNA5/FRT (Invitrogen).
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bioRxiv - Biophysics 2020Quote: The 2019-nCoV receptor-binding domain (RBD) and the N-terminal peptidase domain of human ACE2 were expressed using the Bac-to-Bac baculovirus system (Invitrogen). The 2019-nCoV RBD (residues Arg319-Phe541 ...
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bioRxiv - Immunology 2020Quote: Cells were transduced with matrix-oxGFP lentivirus encoding oxGFP13 with N-terminally fused COX4L mitochondrial matrix targeting sequence and selected with 10μg/mL blasticidin (Thermo Fisher). For experimental use ...
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bioRxiv - Microbiology 2020Quote: ... These two proteins were labeled at the cysteine using thiol-reactive BODIPY™ FL N-(2-Aminoethyl) Maleimide (Thermo Fisher). The proteins were mixed with 10-fold excess BODIPY™ FL N-(2-Aminoethyl ...
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bioRxiv - Microbiology 2020Quote: ... One-step RT-qPCR was performed with primers targeting the SARS-CoV-2 N region (BEI) using the EXPRESS One-Step Superscript qRT-PCR Kit (ThermoFisher) on an Applied Biosystems QuantStudio3 instrument ...
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bioRxiv - Molecular Biology 2021Quote: ... and HL-60 (ATCC, CCL-240) cells were grown in RPMI 1640 medium (Invitrogen, Life technologies, cat n°61870-044). Human A-431 (epidermoid carcinoma ...
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bioRxiv - Molecular Biology 2021Quote: ... and HL-60 (ATCC, CCL-240) cells were grown in RPMI 1640 medium (Invitrogen, Life technologies, cat n°61870-044). Human A-431 (epidermoid carcinoma ...
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bioRxiv - Immunology 2021Quote: The COVID-19 receptor-binding domain (RBD) and the N-terminal peptidase domain of human ACE2 were expressed using HEK293F cells (Invitrogen). The COVID-19 RBD (residues Arg319-Phe541 ...
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bioRxiv - Microbiology 2021Quote: ... the DNA sequence encoding the Nmono (monomeric N mutant K170A/R185A) was cloned by in vitro recombination (Gateway technology; Invitrogen) from pDONR207 into the yeast two-hybrid vector pPC97-GW for expression in fusion downstream of the GAL4 DNA-binding domain (GAL4-BD) ...
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bioRxiv - Physiology 2020Quote: ... 1 mM fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Life Technologies) was diluted in Live Cell Imaging Solution (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: The T7 gp5.9 gene from phage T7 was produced as a synthetic gene construct with an N-terminal 3C-cleavable histidine tag (GeneArt, Invitrogen). This was subcloned into the pACEBAC1 (MultiBac ...
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bioRxiv - Immunology 2022Quote: ... The Hc gene with a N-terminal gp67 signal peptide and Lc gene with a N-terminal honeybee melittin signal peptide were subcloned into pFastBac Dual vector (Invitrogen) containing two multiple cloning sites ...
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bioRxiv - Neuroscience 2022Quote: ... Supernatants were removed and spiked with 50 mM N-ethylmalamide before being added to 50 μL of magnetic protein G Dynabeads (Invitrogen) pre-conjugated with 10 μg mouse HA antibody (in house ...
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bioRxiv - Neuroscience 2022Quote: ... 20 three months old transgenic TgN3R182C150 mice were immunized initially either with 25 µg of aggregated NOTCH3 EGF1-5 protein (n=11, vaccinated) plus adjuvant (Imject Alum Adjuvant, ThermoFisher) or PBS (n=9 ...
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bioRxiv - Neuroscience 2022Quote: ... Maturation medium I was DMEM/F12 supplemented with 100 U/ml Penicillin-Streptomycin and 1% N-2 Supplement (Gibco, 17502048). On day 10 ...
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bioRxiv - Plant Biology 2022Quote: ... Total leaf N concentration was determined with an Organic Elemental Analyzer (Flash EA 112, Thermo Fisher Scientific Inc., MA, USA). Ash concentration was determined gravimetrically after combustion of duplicated samples for at least 4 h at 500 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... The identified N-glycopeptides were quantified using the ‘Report Ion Quantifier’ available as a node in Proteome Discoverer v2.2 (Thermo Scientific) and the reporter ion intensities from the MS/MS scans were extracted from the QuantSpectra table (22) ...
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bioRxiv - Genetics 2024Quote: ... Wild type N/TERT-2G cell line was cultured using EpiLife medium with 60 µM calcium (Gibco™, cat. #MEPI500CA), supplemented with human keratinocyte growth supplement (HKGS ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthetized from 2ug of total RNA using MMLV-Reverse transcriptase and d(N)6 random primers in a 20ul reaction following the manufacturer’s protocol (ThermoFisher Scientific). RT-qPCR analyses were performed in triplicate using 2.5 μl of cDNA ...
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bioRxiv - Cell Biology 2024Quote: ... and pCRSIPR-Lenti-V2 by electroporation using the NEON system (Thermo Fisher, buffer N, 4 pulses, 10 ms, 1230 V). After electroporation ...
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bioRxiv - Neuroscience 2024Quote: ... NPC were plated on poly- L-ornithine/laminin-coated flasks at 50’000 cells/cm2 in2µg/ml laminin-supplemented neural expansion medium (DMEM/F-12 + Glutamax, Gibco®; 1x N-2 supplement, Gibco® ...
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bioRxiv - Microbiology 2024Quote: O/N cultures were diluted 10000-fold in BHI and 150 μl inoculated into a round bottom 96-well plate (Nunc) to initiate biofilm formation in microtiter plates ...
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bioRxiv - Microbiology 2024Quote: ... HEK293FT (293T cells optimized for VLP production, gift from N. Manel) and OMK cells were cultivated in media DMEM (Thermofisher) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... at 4°C o/n and then with 40 μL of pre-blocked (1 mg/ml BSA) Dynabeads protein A (ThermoFisher). Beads were sequentially washed with Wash buffer 1 (20 mM Tris HCl pH 8.1 ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were blocked for 2hrs at RT in 5% milk powder and then incubated overnight (o/n) at 4°C with primary rabbit anti-GFP antibody (1:1,000; Molecular Probes). Membranes were washed 3x for 10min in 1X TBST ...
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bioRxiv - Neuroscience 2023Quote: ... A 6xHis-myc tag or GFP was attached to the N-terminus and the construct was cloned into the pSecTag2A vector (Invitrogen) in frame with the N-terminal IgK signal sequence ...
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bioRxiv - Bioengineering 2024Quote: WT IL-10 and Fab-IL-10 constructs were fluorescently labeled using Alexa Fluor 647 N-hydroxysuccinimide (NHS) ester (Invitrogen), and unreacted dye was removed with a Zeba Spin Desalting Column (7K MWCO ...
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bioRxiv - Biochemistry 2024Quote: ... Labeling of WT and p.R196H actin at cysteine–374 using N–(1–pyrene)iodoacetamide (Thermo Fisher Scientific, Waltham, MA, USA) followed established protocols (55).
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bioRxiv - Neuroscience 2023Quote: ... and incubated in culture media (66% BME, 25% Hanks, 5% FBS, 1% N-2, 1% penicillin, streptomycin and glutamine; all from Invitrogen) and 0.66% D-(+)-Glucose (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Experiments were carried out using 8 μL of SETDB1-3TD (residues 196-403, N-terminal His tag) and 20X concentration Sy-pro Orange dye (5000X DMSO stock, ThermoFisher) and 2 uL of ligand for a final concentration of 20 μM of protein and 200 μM of ligand with 1.1% of DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... total proteins from RVLM-containing tissue punches were extracted using N-PER™ Neuronal Protein Extraction Reagent (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Sequences of full-length Dprs and DIPs with an N-terminal V5 tag were cloned into the pMT/BiP/V5-His A vector (Invitrogen) for expression in S2 Drosophila cells for PLC cleavage assay or into the pAcGP67 baculovirus transfer vector (BD Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... zipper tag via a 5XGGS linker region was co-transfected with N-terminally His-tagged human Leptin in suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
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bioRxiv - Neuroscience 2023Quote: ... and the QOLG-1 wild-type parent line (n=1) were karyotyped using a commercial service that provides information on chromosomal changes >1Mb resolution (KaryoStat Assay, ThermoFisher). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... and once with 1X PBS for 5 min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, ThermoFisher) in NHS-acetate buffer (0.1M NaP ...
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bioRxiv - Immunology 2023Quote: ... which was followed by an overnight incubation at 4°C with N-hydroxysuccinimide ester derivatives of AF647 (Thermo Fisher Scientific) present in two-fold molar excess.
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bioRxiv - Pathology 2023Quote: ... LMD samples (n=5 for each sample structure) were pooled by adding 60 μl of acetonitrile (10055454, Thermo Fisher Scientific) to each tube and spun down ...
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bioRxiv - Cell Biology 2023Quote: ... Domes were polymerised by incubation for 15 minutes at 37°C and Advanced DMEM media supplemented with 1x N-2 (Gibco), 1x B-27 (Gibco) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Secondary antibodies were: goat anti-rat Alexa Fluor 647 (1:500, for anti-N-Cadherin, Life Technologies, cat. no. A21247), goat anti-rabbit Alexa Fluor 488 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: Full-length cDNA sequence of Hs WDR23 Isoforms 1 and 2 were cloned into pcDNA 6.2/N-EmGFP/TOPO (Thermo Fisher), as previously described [19] ...
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bioRxiv - Molecular Biology 2023Quote: ... and from the liver graft of living donors (N=4) were obtained at the time of liver transplantation and stored frozen in RNAlater solution (Invitrogen). ABCB4 pathogenic mutations were documented in all PFIC3 by genetic analysis ...
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bioRxiv - Immunology 2023Quote: ... and stained for SARS-CoV-2 N protein using Biotin-labelled-CR3009 antibody produced in-house together with a Streptavidin-Alexa488 (S32354, Invitrogen) and cellular DNA using DAPI (10236276001 ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was changed to neural induction media (1:1 mixture of DMEM/F-12 GlutaMAX: Neurobasal media supplemented with 0.5x N-2 (Life Technologies), 0.5x B-27 (Life Technologies) ...
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bioRxiv - Immunology 2022Quote: ... sheared gDNA of each sample was split in two duplicates and samples were incubated o/n at 65°C after mixing with an equal volume hybridization buffer (1 ml composition: 667 µl 20x SSPE (Gibco); 267 µl 50x Denhardt’s solution (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2022Quote: ... formalin-fixed fibrotic capsule tissue samples (n=2 per group) and subcutaneous tissue samples (n=2) were placed in a solution of 2 mg/ml FITC (Invitrogen) in DMEM/F12 media (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... Cy3B and AF647 free dyes were prepared by hydrolyzing the corresponding NHS ( N-hydroxysuccimidyl) esters (PA63101, Cytiva, and A200006, Invitrogen) in 0.1 M sodium bicarbonate overnight ...
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bioRxiv - Genetics 2023Quote: ... Quantitative real time PCR (n = 7–8 per genotype) was performed on an ABI 7300 Sequence Detection system (Applied Biosystems) using the PowerUp SYBR Green Master Mix Kit (Applied Biosystems).