Labshake search
Citations for Thermo Fisher :
7951 - 8000 of 10000+ citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
-
bioRxiv - Neuroscience 2023Quote: ... 30 mg of tissue per sample was homogenized in N-PER Neuronal Protein Extraction Reagent (Thermo Fisher Scientific, Cat. # 87792) with protease and phosphatase inhibitors (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2023Quote: ... For C-terminal fusions the CDS of PYE and OLV versions were amplified from cDNA of Fe deficient Arabidopsis WT roots with primer pairs PYE_B1 fw/PYEns_B2 rev and OLV_B1 fw/OLVns_B2 rev carrying B1 and B2 attachment sites without stop codon or from existing pDONR with N-terminal fusion (Supplemental Table S1) and transferred via Gateway cloning into pDONR207 (Invitrogen) according to the manual (Gateway ...
-
bioRxiv - Molecular Biology 2023Quote: ... The flow-through was dialysed O/N at 4°C in a SnakeSkin™ Dialysis Tubing (3.5K MWCO, Thermo Scientific) against dialysis buffer (50 mM Tris-HCl (pH 8.0) ...
-
bioRxiv - Bioengineering 2023Quote: Blood plasma extracted from each group (n=5) was analyzed using the Inflammation 20-Plex Human ProcartaPlex™ Panel (Invitrogen). Briefly ...
-
bioRxiv - Biochemistry 2024Quote: ... Labeling of WT and p.R196H actin at cysteine–374 using N–(1–pyrene)iodoacetamide (Thermo Fisher Scientific, Waltham, MA, USA) followed established protocols (55).
-
bioRxiv - Bioengineering 2024Quote: WT IL-10 and Fab-IL-10 constructs were fluorescently labeled using Alexa Fluor 647 N-hydroxysuccinimide (NHS) ester (Invitrogen), and unreacted dye was removed with a Zeba Spin Desalting Column (7K MWCO ...
-
bioRxiv - Developmental Biology 2023Quote: ... Membranes were blocked for 2hrs at RT in 5% milk powder and then incubated overnight (o/n) at 4°C with primary rabbit anti-GFP antibody (1:1,000; Molecular Probes). Membranes were washed 3x for 10min in 1X TBST ...
-
bioRxiv - Neuroscience 2023Quote: ... A 6xHis-myc tag or GFP was attached to the N-terminus and the construct was cloned into the pSecTag2A vector (Invitrogen) in frame with the N-terminal IgK signal sequence ...
-
bioRxiv - Genetics 2024Quote: ... Wild type N/TERT-2G cell line was cultured using EpiLife medium with 60 µM calcium (Gibco™, cat. #MEPI500CA), supplemented with human keratinocyte growth supplement (HKGS ...
-
bioRxiv - Molecular Biology 2023Quote: ... as already described [17] and reseeded onto 0.2 mm bone slices labelled with N-hydroxysuccinimide ester-activated rhodamine fluorescent dye (ThermoFisher Scientific), at a cell density of 1x105 cells/bone slice (Boneslices.com) ...
-
bioRxiv - Developmental Biology 2024Quote: The PROX1 cDNA was subcloned into pFASTBAC1 to express PROX1 with an N-terminal FLAG- tag in Sf9 cells by the baculovirus expression system (Invitrogen). The PROX1-FLAG protein was then purified on M2 agarose (Sigma) ...
-
bioRxiv - Biochemistry 2024Quote: ... was serial diluted using a solution containing 0.1% FA in LCMS grade water (Pierce 85170) and 0.1% n-Dodecyl-beta-Maltoside Detergent (DDM, Thermo Fisher, 89902). A total of 10 microliters of each dilution ...
-
bioRxiv - Biochemistry 2024Quote: BRSK1 and 2 were cloned into pDest vectors (to express N-terminal Flag or HA tagged proteins) using the Gateway LR Clonase II system (Invitrogen) as per the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2024Quote: ... The percentage of C and N were quantified using the FLASH 2000 Organic Elemental Analyzer (Thermo Fisher Scientific Villebon, France) and the 15N enrichment using the Delta V Advantage isotope ratio mass spectrometer (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... n=15 full larvae) or extracted from hCMEC/D3 cells seeded in 12 well/plates using a standard TRIzol (Invitrogen) method ...
-
bioRxiv - Biochemistry 2024Quote: ... The DNA synthesis of calpL fused with TEV-cleavable His6-StrepII-His6 tag at the N- or C-terminal and cloning into pBAD/HisA vector (Invitrogen) was ordered from Twist Bioscience ...
-
bioRxiv - Neuroscience 2024Quote: ... Full-length mouse GPR158 containing an N-terminal HA-tag or smURFP-tag were cloned into pcDNA3.1 (Thermo Fisher Scientific). Full-length cDNA encoding mouse PLCXD2 containing a C-terminal FLAG-tag in pcDNA3.1 (clone OMu06191 ...
-
bioRxiv - Cell Biology 2024Quote: ... WT and YAP1-KO EHTs were snap frozen in liquid nitrogen and stored at −80 °C before thawing them O/N at −20 °C in RNA LATER-ICE (ThermoFisher). RNA was extracted using High Pure RNA Isolation Kit (Roche ...
-
bioRxiv - Biophysics 2020Quote: ... The LACV-L-expressing baculovirus was generated via the standard Bac-to-Bac method (Invitrogen). For large scale expression ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1% L-glutamine and 1% MEM Non-essential amino acids (MEM NEAA) (Gibco, 11140-050). For SaOS-2 cells ...
-
bioRxiv - Biochemistry 2021Quote: XTC cells were cultured in 70% Leibovitz’s L-15 medium (Thermo Fisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (FBS) ...
-
A lateral protrusion latticework connects neuroepithelial cells and is regulated during neurogenesisbioRxiv - Developmental Biology 2021Quote: ... Explants were embedded in collagen (supplemented with 0.1% acetic acid, 5x L-15 medium [ThermoFisher, 41300] and 7.5% sodium bicarbonate [ThermoFisher ...
-
bioRxiv - Cell Biology 2020Quote: ... supplemented with 2 mM L-glutamine and 10% heat-inactivated fetal bovine serum (FBS; Gibco) at 37°C ...
-
bioRxiv - Cell Biology 2020Quote: ... and goat anti-mouse IgG (H+L) Alexa Fluor 680 (1:10,000 Thermofisher, A-28183). Stained membranes were imaged on an Odyssey instrument (LICOR).
-
bioRxiv - Cell Biology 2019Quote: ... Alexa 647-conjugated goat anti-mouse IgG (H+L) antibody (no. A-21235, Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2020Quote: ... before resuspension in Zebrafish Neural Medium (ZNM) consisting of Leibovitz L-15 medium (Gibco-11415049), 1X N-2 Supplement (Gibco-17502048) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Samples from surgically removed aborted fetal tissues were collected into Leibovitz’s L-15 (11415064, Gibco) plus with 10% fetal bovine serum (FBS ...
-
bioRxiv - Developmental Biology 2020Quote: ... Alexa Fluor 488 goat anti-rat IgG (H + L, Thermo Fisher Scientific Cat# A-11006), CF488A goat anti-rat IgG (H + L ...
-
bioRxiv - Developmental Biology 2020Quote: ... and 1 µg/ml heparin in RPMI 1640 with L-Glutamine (ThermoFisher Scientific 21875-034), with 70% of the medium pre-conditioned on mouse embryonic fibroblasts (CM) ...
-
bioRxiv - Neuroscience 2021Quote: ... and samples were washed and resuspended in 500μL cold Leibovitz’s L-15 media (Thermo Fisher) with 4μL DNAse I (Invitrogen ...
-
bioRxiv - Neuroscience 2021Quote: ... coated with 0.1% Poly-L- ornithine and Laminin (5 ug/mL; Thermo Fisher Scientific, 23017015). On DIV 5 ...
-
bioRxiv - Neuroscience 2021Quote: ... and the uterus was removed and placed into chilled Leibovitz’s L-15 Medium (ThermoFisher, 11415064) with Penicillin/Streptomycin (0.2%) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated with a goat anti-mouse IgG antibody (H+L; Invitrogen, A-11032) conjugated with Alexa Fluor 594 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were grown at 28 degrees Celsius in Leibovitz’s L-15 +glutamine Medium (Invitrogen, 21083027) containing 15% heat inactivated fetal bovine serum (FBS ...
-
bioRxiv - Genomics 2020Quote: ... and 380 mg/L EGTA [MP] at pH 8.5) and 0.9 mL phenol/chloroform (Fisher Scientific). After phase separation ...
-
bioRxiv - Immunology 2021Quote: ... and synchronized by subjecting to starvation conditions by adding starvation media (L-15 media (Gibco) with 1% penicillin-streptomycin and 1% L-glutamine ...
-
bioRxiv - Immunology 2019Quote: ... and then stained with Alexa 647-conjugated anti-human IgG (H+L) antibody (Thermo Fisher) for 30 min at 4°C ...
-
bioRxiv - Neuroscience 2019Quote: ... or Alexa Fluor 568 donkey anti-mouse IgG (H+L) (1:500, #A10037, Life Technologies), respectively ...
-
bioRxiv - Neuroscience 2019Quote: ... or Alexa Fluor 568 donkey anti-mouse IgG (H+L) (1:500, #A10037, Life Technologies), respectively ...
-
bioRxiv - Immunology 2020Quote: ... The following secondary antibodies were used: alexa 488 donkey anti-mouse IgG (H+L) (Invitrogen, Eugene ...
-
bioRxiv - Cell Biology 2019Quote: ... half of the cells were cultured in in high (4.5g/L) glucose DMEM (Thermo Fisher), supplemented with 5% v/v fetal bovine serum (FBS) ...
-
bioRxiv - Neuroscience 2019Quote: ... or Alexa Fluor 594 goat anti-mouse IgG (H+L) (1:1000, Molecular Probes, #A11005). Sections were washed 4 times in PBS each for 10 minutes at room temperature and subsequently mounted on glass slides in Vectashield with DAPI (H-1200 ...
-
bioRxiv - Neuroscience 2019Quote: ... or an Alexa Fluor 488-conjugated goat anti-mouse IgG (H+L) (1:1,000; Invitrogen) and 4′,6-diamidino-2-phenylindole (DAPI) ...
-
bioRxiv - Microbiology 2019Quote: ... JAR4 binding was detected using FITC-conjugated rat anti-mouse IgG (H+L) (Thermo Fisher) at a 1:50 dilution for an incubation period of 1 h ...
-
bioRxiv - Cancer Biology 2019Quote: Lewis and TβRI immunoprecipitations were performed using PierceTM Protein L Magnetic Beads (ThermoFisher Scientific, #88849) and PierceTM Protein A/G Magnetic Beads (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor® 568 goat anti-rabbit IgG (H+L) both diluted 1/300 (Invitrogen)) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4 mM L-glutamine) and used to infect 80-100 % confluent HEK293-TRex cells (ThermoFisher) grown in 96-well plates (BD Purecoat Amine ...
-
bioRxiv - Cell Biology 2019Quote: hMSCs were isolated from umbilical cord tissue and cultured in L-DMEM (Gibco, 11885-084) medium containing 15% FBS (Hyclone ...
-
bioRxiv - Genetics 2020Quote: C6/36 cells (ATCC: CRL-1660) were cultured in Leibovitz’s L-15 medium (Gibco, 41300039) supplemented with 10% Fetal Bovine Serum (FBS ...