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Citations for Thermo Fisher :
7801 - 7850 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Cancer cells were isolated from the tumor cell pellets and dissociated into single tumor cells with TrypLE Express Enzyme (#12605010, Gibco) containing DNase I at 37 °C for 10 minutes.
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bioRxiv - Cell Biology 2019Quote: ... Islets were then collected and dispersed into single cells using TrypLE Express to measure cell number for normalization using Countess Automated Cell Counter (Invitrogen). Insulin content was determined using Mouse Insulin ELISA kits (ALPCO Diagnostics).
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bioRxiv - Genomics 2021Quote: ... Washed cells were immediately counted in a Trypan blue stain-based Cell Countess II Automated Cell Counter (Thermo Scientific #AMQAX1000).
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bioRxiv - Microbiology 2022Quote: ... The percentage of transfected or transduced cells (GFP+ cells) was determined by gating the living cell population based on viability dye staining (Aqua Vivid, Invitrogen). Samples were acquired on an LSRII cytometer (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and 125) with TSt-4 (B cells) or TSt-4/DLL1 stromal cells (T cells) in RPMI (Thermo Fisher Scientific) supplemented with 10% BSA (093001 ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were then resuspended at 2 × 106 cells/ml in PBS and stained with Cell Trace Violet (1:2000, Invitrogen) for 20 minutes at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... before co-culture T cells were resuspended 1×106 cells/mL in 1X PBS containing Celltrace Violet Cell (cat#C34557 from Thermofisher) for 20 minutes at 37°C ...
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bioRxiv - Bioengineering 2022Quote: Murine MC38-RFP adenocarcinoma cells and MC38 hybrid cells (MC38-macrophage hybrid cells)8 were cultured in Dulbecco’s modified eagle medium (DMEM) (Thermo Fisher) supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... protein was evaluated in lysate cells and the type of cell death was evaluated in detached cells after treatment with trypsin (Gibco). For treatment experiment ...
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bioRxiv - Molecular Biology 2024Quote: ... an initial cell count cell was conducted using cell-permeable DNA stain Hoechst 33342 (Invitrogen, H3570, 1:1000, 10µg/mL). Whole wells were stained and imaged in triplicate every day for three days using a 2X objective (Nikon ...
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bioRxiv - Cell Biology 2023Quote: ... GFP and FLPo cells were trypsinized and the cell concentration was determined using a Countess Automated Cell Counter (Thermofisher, USA). The final concentration was the average of three reads from 3 different aliquots of cell suspension ...
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bioRxiv - Immunology 2024Quote: ... Red blood cells were lysed in single-cell suspension of bone marrow cells using ACK Lysing Buffer (Thermo Fisher Scientific) and granulocytes were separated from mononuclear cells using 70%/86% Percoll gradient at interphase ...
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Single-cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastomabioRxiv - Cancer Biology 2023Quote: ... a single-cell suspension was created by treating the GBM cells for 3 minutes with accutase (StemPro Accutase Cell Dissociation Reagent, Gibco) at 37°C.
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bioRxiv - Cell Biology 2023Quote: ... Cell division kinetics were carried out by tracking cell divisions of single sorted cells into 60-well plates (ThermoFisher Scientific) for 3 days post-sort ...
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bioRxiv - Cell Biology 2023Quote: ... Adherent cell lines were maintained by first rinsing cells with 1x PBS and then treating cells with TrypLE (ThermoFisher Scientific) for a maximum of 5 minutes at 37℃ before quenching the reaction with complete media ...
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bioRxiv - Cancer Biology 2023Quote: PD-L1 expression on tumor cells: A549 cells incubated with stimulated T cells were also analyzed for PD-L1 expression (APC, Invitrogen) and viability with 7AAD staining.
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability of A2780 cells after CDDP treatment was evaluated by the Live/Dead Cell Stain Kit (InvitrogenTM, ThermoFisher Scientific) by flow cytometry (BD FACS CantoII ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were filtered through a 100 µm cell strainer and red blood cells were lysed using ACK buffer (A1049201, Gibco). Cells were washed in PBS ...
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bioRxiv - Cell Biology 2023Quote: The following antibiotics were used to select cell lines: Puromycin (1 μg/ml for HeLa cells and μg/ml for HEK293T cells; A1113803; Gibco) and Blasticidin (15 μg/ml for HeLa cells and 5 μg/ml for HEK293T cells ...
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bioRxiv - Cancer Biology 2023Quote: ... We generated two LTED variants A and B cells from their corresponding parental cell lines by chronically passaging parental cell lines in phenol red-free IMEM media (Gibco) supplemented with 10% charcoal-stripped bovine serum (CSS ...
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bioRxiv - Biochemistry 2023Quote: ... and used to infect Sf21 insect cells at a cell density of 1.0 x 106 cells/mL in Sf-900 SFM medium (Gibco Life Technologies), shaking at 90 rpm on Sartorius Stedim Biotech “CERTOMAT RM” with a 25mm orbit ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were stained with CellTrace™ CFSE Cell Proliferation Kit (Invitrogen™) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: The number of live cells was determined by Countess cell counter (ThermoFisher) or manually by averaging the number of cells in 4 1.3×1.75mm2 fields for each 10cm plate under light microscopy ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were immersed in Live Cell Imaging Solution (Thermo Fisher Scientific, Inc.) before imaging ...
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bioRxiv - Cell Biology 2020Quote: ... one cell population was labelled with CMRA cell tracker dye (ThermoFisher Scientific) as previously described9 ...
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bioRxiv - Cell Biology 2020Quote: ... and cells were plated in 75 cm2 cell culture flask (T75; Invitrogen) and placed in a humidified incubator (37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Cell viabilities (>85%) were measured using the Automated Cell Counter (ThermoFisher# AMQAF1000). Single-cell droplet libraries from this suspension were generated in the 10XGenomics Chromium controller (∼2000 target cell recovery/group ...
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bioRxiv - Immunology 2021Quote: ... Cell viability was tested with prestoBlue cell viability reagent (Thermo Fisher Scientific) according to the manufacturer’s protocols ...
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bioRxiv - Genomics 2020Quote: ... Cells were dissociated into single cells with TrypLE Express (Thermo Fisher, 12604013).
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bioRxiv - Genetics 2021Quote: ... aliquots of cells (100,000-500,000 cells) were lysed in TRIzol (Life Technologies) for total RNA extraction ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The cells were counted using Countess II Automated Cell Counter (ThermoFisher Scientific). The total live cell count/ml was recorded as the final number of cells and the growth rate was calculated as Nf = N0 2ηt ...
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bioRxiv - Microbiology 2020Quote: ... cells were harvested using an enzyme-free Cell Dissociation buffer (13151014, Thermofisher). All incubations were performed at 4°C in PBS/BSA 3% during one hour with either “αH” anti-SR-B1 polyclonal rabbit serum (40 ...
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bioRxiv - Microbiology 2019Quote: ... coli BL21 cells were stained with VybrantTM DiO live cell tracer (ThermoFisher). Cell-labeling solution (5 μl ...
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bioRxiv - Immunology 2020Quote: ... Cells were stimulated with 200 µL 1× eBioscience Cell Stimulation Cocktail (ThermoFisher) without protein transporter inhibitor for 1 hr at 37 °C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... cells were dissociated into single-cell using TrypLE Express (Thermo Fisher Scientific) and cultured in N2B27 media [1:1 mixture of N2 media and Neurobasal media (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... BAL cells were incubated with Red Blood Cell Lysis buffer (Fisher Scientific) for 5 mins at RT before washing with PBS+ 0.5% BSA and centrifugation at 350 rcf for 5 mins at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... LNCaP cells (Prostate Cancer Cell) were cultured in T-Medium (Life Technologies) supplemented with 10% FBS and 2mM L-glutamine and passaged when 90-100% confluent ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were counted using the Countess II FL Automated Cell Counter (ThermoFisher). Routine light microscopy was performed using the EVOS FL Cell Imaging System (ThermoFisher ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were passaged using TrypLE cell dissociation enzyme (Thermo Fisher Scientific, 12504013) following manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2019Quote: Transduction of HTT in Mammalian Cells: EXPI293F cells (Thermo Fisher, Cat. A14527) were maintained in EXPI293 Expression Medium (Thermo Fisher ...
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bioRxiv - Immunology 2019Quote: ... Single cells suspension was filtered through 100μm nylon cell strainer (Fisher Scientific) and cells were pelleted by centrifugation at 1500 rpm for 5 minutes and resuspended in 1 ml of 1xPBS ...
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bioRxiv - Cancer Biology 2019Quote: Cells were detached with CellStripper non-enzymatic cell dissociation reagent (Fisher Scientific). Viability was assessed using the Molecular Probes LIVE/DEAD stain kit with excitation at 405 nm (20 min ...
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bioRxiv - Cancer Biology 2019Quote: ... All cell pellets were stored cryopreserved in cell-freezing media (Gibco #12648).
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bioRxiv - Immunology 2021Quote: ... The resultant cell suspensions were filtered through 70µm cell strainers (ThermoFisher Scientific) and centrifuged at 400 x g for 5 mins ...
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bioRxiv - Microbiology 2021Quote: ... Cells were surface stained with Fixable Blue Dead Cell Stain (Life Technologies) and marker-specific fluorochrome-labeled antibodies (Supplementary Table 1) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell viability was controlled using the LIVE/DEAD cell imaging kit (ThermoFisher) following manufacturers instruction ...
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bioRxiv - Cell Biology 2021Quote: ... Cell viability was controlled using the LIVE/DEAD cell imaging kit (ThermoFisher).
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bioRxiv - Genomics 2021Quote: ... HEK293T cells and K562 cells were cultured in DMEM High glucose (GIBCO) and RPMI 1640 medium (GIBCO) ...
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bioRxiv - Immunology 2021Quote: ... T cells were stained with Cell Trace Violet dye (ThermoFisher, cat. #C34557) washed twice with RPMI 1640 (10% FCS ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed with cell media (DMEM High, Thermo Fisher Scientific, USA) that contain only 1% PSA (Pan Biotech Germany) ...