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Citations for Thermo Fisher :
7851 - 7900 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... cell pellets were treated with 1X Red Blood Cell Lysis (Invitrogen) for 10 min at room temperature ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used an automated cell counter (Cell Countess II, Thermo Fisher) to calculate the number of spores and the average spore size in each fruiting body ...
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bioRxiv - Bioengineering 2024Quote: ... Untreated cells and cells treated with Lipofectamine RNAiMAX (L-iMax, Invitrogen) carrying Cy5-siRNAmi were used as controls ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were harvested as single cells with 1X TrypLE select (Gibco) during splits and counted using the Countess cell counter (Thermo Fisher Scientific).
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bioRxiv - Genetics 2024Quote: ... Cells were then lyzed in Cell Extraction buffer (Invitrogen CAT#FNN001). Sample concentrations were adjusted to 4mg/ml protein using protein quantification measurements (Pierce™ BCA Protein Assay Kit ...
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bioRxiv - Cancer Biology 2021Quote: ... in CD-1 mouse, Sprague−Dawley rat, and human plasma (Bioreclamation, Inc., Hicksville, NY) by equilibrium dialysis using the RED Device (Thermo Fisher Scientific, Rockford, IL). Compounds were added to pooled plasma (n ≥ 3 ...
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bioRxiv - Developmental Biology 2021Quote: ... the causal relation that a target gene is up- or downregulated depending on the transcription complex being active or inactive and (ii) the causal relation that a signal from a gene expression microarray (e.g. Affymetrix Human Genome U133 Plus 2.0) probeset is high or low depending on the target gene being up or down ...
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bioRxiv - Cell Biology 2020Quote: ... α-SC35 (described previously98, Mouse 1:1000), α-NIN (Institut curie, Human 1:200), anti-Arl13b (proteintech, Rabbit, 1:4500) α-Rabbit Alexa594 (ThermoFisher, 1:400), α-Mouse IgG1 Alexa488 (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: Total RNA extracted from EndoC-βH1 and collagenase-isolated human pancreatic islets samples was quantified using Qubit 3000 Fluorometer (ThermoFisher Scientific, Waltham, MA, USA), while those extracted from LCM-islets were quantified using 2100 Bioanalyzer-RNA 6000 Pico Kit (cat ...
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bioRxiv - Developmental Biology 2021Quote: ... For cells freshly purifed from human lung tissues were directly lysed using 100 μl lysis buffer from PicoPure™ RNA Isolation Kit (Thermo Fisher Scientific, KIT0204). First Strand cDNA synthesis was performed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... according to manufacturer’s protocol Following ChIP-valuated antibodies were used for precipitation: mouse-anti-human H3K9/14/18/23/27ac (Thermo Fisher Scientific, Carlsbad, CA, USA) and mouse-anti-IgG (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Blots were probed with primary antibodies: human anti-SARS-CoV-2 Spike (S-ECD/RBD) (cat# bcb03, Thermo Fisher, 1:1000, non-reducing conditions), anti-Salmonella typhimurium LPS (cat# ab8274 ...
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bioRxiv - Molecular Biology 2020Quote: ... falciparum asexual blood stages were cultured in human erythrocytes (National Blood Transfusion Service, London, United Kingdom) and complete medium (CM) consisting of RPMI-1640 medium (Life Technologies, California, United States) supplemented with 0.5% AlbuMAX type II (Gibco) ...
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bioRxiv - Genomics 2020Quote: ... Human DNA was removed using DNase I and RNA concentration was measured using Qubit RNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). DNA-depleted and purified RNA was used to construct double-stranded DNA library using MGIEasy RNA Library preparation reagent set (MGI ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated for 48h at 4°C in the same solution with mouse anti-NeuN (1:2000; Chemicon; MAB377, Temecula, CA, USA) and anti-human PHF-Tau antibody clone AT8 (1:2000, MN1020, Thermo Scientific, Waltham, MA, USA); for the sake of clarity ...
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bioRxiv - Cancer Biology 2020Quote: Human CD34+ cells were isolated from patient bone marrow samples by erythrocyte lysis buffer treatment, followed by separation with AutoMACs sorter and CD34 Microbead Kit human (MiltenyiBiotech, Germany) and grown in IMDM (Gibco, Thermo Fisher Scientific, CA, USA) supplemented with 2% FBS (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... The iPSCs were cultured on irrMEF with medium for human iPSCs : KnockoutTM-Dulbecco’s modified Eagle’s medium (KO-DMEM: 10829-018, Life Technologies; Thermo Fisher Scientific, MA, USA) supplemented with 20% KnockoutTM-Serum Replacement (KO-SR ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed 5 times and incubated with goat anti-human IgG (H+L) antibody conjugated with ALP (Thermo Fisher, a18808, 1:5000) for 30 min at 37 °C ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... The fluorescent secondary antibodies used in this study were Alexa Fluor 568 goat anti-human IgG(H+L) (Molecular Probes, A-21090, 1:1000), Alexa Fluor 488 goat anti-mouse IgG(H+L ...
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bioRxiv - Microbiology 2020Quote: ... HK-2 cells were maintained in keratinocyte serum-free (KSF) media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Cancer Biology 2020Quote: Peptide spectrum matching of MS/MS spectra of each file was searched against the human database (UniProt) using the Sequest algorithm within Proteome Discoverer v 2.3 software (Thermo Fisher Scientific, San Jose, CA). The Sequest database search was performed with the following parameters ...
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bioRxiv - Cell Biology 2022Quote: Plasmodium falciparum 3D7 strain parasites were cultured within human O+ erythrocytes maintaining 4% haematocrit in RPMI 1640 medium (Invitrogen Corp., San Diego, CA, USA) supplemented with Albumax I (0.5% ...
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bioRxiv - Biochemistry 2020Quote: ... The human Bip 19-654aa (referred to as Bip)-encoding DNA was cloned into a modified pProEx™-HTb vector (Thermo Fisher Scientific Inc.), resulting in a construct with a N-terminal 6xHis tag ...
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bioRxiv - Microbiology 2021Quote: ... A donkey anti-mouse IgG-Alexa Fluor 555 antibody (SG2-2E6) or goat anti-human IgG-Alexa Fluor 488 antibody (93k) (Life Technologies, Grand Island, NY) was used to detect bound anti-VZV gB mAb ...
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bioRxiv - Molecular Biology 2020Quote: ... driven by the human cytomegalovirus (CMV) immediate early enhancer and promoter was assembled using the GeneArt CRISPR Nuclease Vector system (Thermo Fisher Scientific, Waltham, MA), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: After the skin flap surgery and 15 min prior to the tail-vein injection of GFP-expressing bacteria (107 CFU in 100 μl 1x PBS) and PE-conjugated anti-human CD62E (6 μg, Thermofisher Scientific, #12-0627-42), 100 μg Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin ...
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bioRxiv - Microbiology 2021Quote: ... The technical replicates of the retrieved RNA were pooled prior rRNA depletion, which was performed using the human riboPOOL™ (siTOOLsBiotech, Germany) and Streptavidin-coated magnetic beads (Thermo Fisher Scientific, Norway) following the two-step protocol of the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: ... The presence of secreted Fc Fusion protein in the medium was confirmed by immunoblotting with goat anti-human IgG antibody (Invitrogen, A-21433, 1:500).
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bioRxiv - Immunology 2021Quote: ... Peptide spectrum matching of MS/MS spectra of each file was searched against the human database (UniProt) using the Sequest algorithm within Proteome Discoverer v 2.3 software (Thermo Fisher Scientific, San Jose, CA).
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bioRxiv - Immunology 2022Quote: Human monocytes (20 x 106) were lysed in 650 µl of IP Lysis/Wash buffer (Pierce Direct IP Kit, Thermo Fisher Scientific, Cat. #26148) and the supernatants of cell lysates after centrifugation were transferred to columns containing the TBK1 antibody linked to AminoLink Plus Coupling Resin to pull down the proteins interacting with TBK1 using the Pierce Direct IP Kit according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... We performed confocal reflectance microscopy on unlabeled fibrin samples (see Fig. 2) and fluorescence imaging (see Fig. 4) on networks polymerized from unlabeled fibrinogen and Alexa488-labeled human fibrinogen (Life Technologies, Bleiswijk, the Netherlands) mixed in a 30:1 molar ratio.
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human IFNγ (285-IF) was purchased from R & D Systems (Minneapolis, MN) and mouse IFNγ (BMS326) from eBioscience (Thermo Fisher Scientific, Waltham, MA). The following antibodies were used for Western blot ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed 4X in PBST and subsequently incubated with secondary antibodies (goat anti-rabbit IgG 488, Invitrogen, Carlsbad, CA; #A-11034, 1:4000; & goat anti-human 647, Invitrogen #A-21445, 1:4000) for 1 hour at room temperature ...
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bioRxiv - Genetics 2020Quote: ... Human DNA was removed using DNase I and RNA concentration was measured using Qubit RNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). After human DNA-depletion ...
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bioRxiv - Developmental Biology 2020Quote: ... the ells were transfected with human EC-SOD (hEC-SOD) cDNA inserted into a vector plasmid pcDNA3 (5446 nucleotides; Invitrogen Life Technologies, Carlsbad, CA, USA) as previously described,14 using the FuGENE kit (Roche Diagnostics ...
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bioRxiv - Biophysics 2020Quote: The plasmid pT7-7 containing human aSyn cDNA was transformed into Escherichia coli One Shot® BL21 (DE3) Star™ (Thermo Fisher Scientific, USA). 0.5 L cultures of E ...
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bioRxiv - Immunology 2021Quote: ... Transduction efficiency was determined between days 8-10 of culture using a polyclonal goat anti-human IgG Fc PE (ThermoFisher Scientific, #12-4998-82) and the synaptic transfer to BSLB addressed immediately.
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a Fc part (hinge-CH2-CH3) of human IgG1 for purification was inserted into pFUSE-mIgG1-Fc2 vector (Invitrogen, Thermo Fisher Scientific, MA, USA). The construct (RBD-huFc ...
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bioRxiv - Immunology 2021Quote: ... and then incubated with a 50-fold dilution of PE-conjugated goat anti-human IgM or IgG (both from Thermo Fisher, Waltham, MA, US) for 30 minutes at room temperature ...
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bioRxiv - Pathology 2023Quote: ... Residual human IgG in the DDS and in the ocular tissues was determined using goat anti-human IgG antibody (A-21089, Thermo Scientific, Waltham, MA, USA). The total number and area of cells expressing CD45 in corneal sections were determined using the “Analyze Particles” tool in ImageJ software ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated at 37°C in Human Complete Feeding Medium (hCPLT) consisting of the following: Advanced DMEM/F12 (Thermo Fisher Scientific, Waltham, MA, USA), HEPES (final concentration ...
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bioRxiv - Immunology 2023Quote: ... Quantitative gene expression for mouse and human genes was performed using TaqMan probes and TaqMan Fast Advanced Master Mix (Applied Biosystems/Thermo Fisher, 4444554) with a QuantStudio 6 Pro (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Cancer Biology 2024Quote: ... 25 µL of supernatant was analyzed using Cytokine 35-Plex Human Panel for the Luminex™ platform (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) on the Luminex MAGPIX ...
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bioRxiv - Immunology 2024Quote: Human monocytes (20 x 106) were lysed in 650 µl of IP Lysis/Wash buffer (Pierce Direct IP Kit, Thermo Fisher Scientific, Cat. #26148) and the supernatants of cell lysates after centrifugation were transferred to columns containing the TBK1 antibody linked to AminoLink Plus Coupling Resin to pull down the proteins interacting with TBK1 using the Pierce Direct IP Kit according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... A portion of the Fc blocked-cells were separated to accommodate the unlabeled control while remaining samples were incubated with mouse anti-human HLA-ABC W6/32 (Invitrogen [MA5-11723], 1:40) on ice for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... then 50 μl of sample or standard was added per well (in duplicate) with premixed beads in a 96-well plate according to the manufacturer instructions (ThermoFisher Procartaplex Human Simplex kit). The plate was incubated overnight on a shaker at 4°C ...
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bioRxiv - Immunology 2022Quote: ... genes encoding single chain (sc) Fab and scFc linked to human apoferritin light chain monomers were synthesized and cloned by Geneart (Thermo Fisher Scientific, Waltham, MA) into the pcDNA3.4 expression vector ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then washed once with 150 μL 1% BSA/PBS and then incubated with 50 μL Goat anti-Human IgG Fc secondary antibody PE (ThermoFisher, cat #12-4998-82) at a final concentration of 2.5 μg/mL in 1% BSA/PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...