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Citations for Thermo Fisher :
7601 - 7650 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... adult AB zebrafish olfactory rosettes were dissected and placed directly into 5 ml of Neurobasal medium (Gibco, 21103049) supplemented with 10% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2020Quote: ... 5-ethynyl-2’-deoxyuridine (EdU)) staining were performed using the Click-iT Plus EdU Assay kit (C10339; Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... HEK293T and THP-1 cells were cultured in an incubator at 37C and 5% CO2 in Dulbecco’s modified Eagle’s Medium (DMEM) containing phenol red (Invitrogen) or Rosewell Parks Memorial Institute (RPMI ...
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bioRxiv - Neuroscience 2019Quote: ... in Iscove’s modified Dulbecco’s medium (IMDM) containing 5% fetal calf serum using TurboFect transfection reagents (Thermo Fisher Scientific). 16 hours after transfection ...
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bioRxiv - Developmental Biology 2019Quote: ... Chromatin was pre-cleared with 5 or 10 µl of 1:1 protein A:G Dynabeads (Life Technologies #10015D) and immunoprecipitated with H3K4me1 (Abcam #ab8895) ...
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bioRxiv - Molecular Biology 2020Quote: ... Stock cultures were grown in medium containing 5 mL of RPMI 1640 (GIBCO BRL, Gaithers-Burg, MD, USA) supplemented with 5 % fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2019Quote: ... Enzymatic digestion was inhibited by addition of perfusion buffer containing 5 % (v/v) FBS (FBS; Thermo Fisher, UK). Isolated cardiomyocytes were reintroduced to Ca2+ by three rounds of 20 minutes sequential gravity settling in perfusion buffer containing 300 μM ...
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bioRxiv - Bioengineering 2020Quote: ... The supernatant was harvested 5 days after transfection and purified with a CaptrueSelect KappaXL Affinity Matrix (Thermo Scientific) followed by size exclusion chromatography on a Superdex200 Increase column (GE Healthcare).
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bioRxiv - Genetics 2020Quote: ... and in some cases day 5 (120 hours) by staining with SYBR Green 1 nucleic acid stain (Invitrogen, ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... for 1-3h at 37°C in a HERAcell 150i or 160i 5% CO2 incubator (Thermo Fisher Scientific). Cells were washed in 1x PBS and fixed in 10% neutral buffered formalin (HT501128 ...
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bioRxiv - Microbiology 2020Quote: ... falciparum were maintained at 5-10% parasitaemia in RPMI1640 supplemented with 0.5% Albumax II (RPMI 1640-Albumax; ThermoFisher) (Trager and Jensen,1976) ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNAs were synthesized from 5 µg total RNA using the SuperScript II first-strand cDNA synthesis system (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were grown under standard tissue culture conditions (37°C, 5% CO2) in RPMI medium (GIBCO Cat# 31870025), supplemented with 10% FBS ...
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bioRxiv - Plant Biology 2021Quote: ... The bands were detected by using SuperSignal pico/femto chemiluminescence kits (Thermo Scientific; #’s1859022/3 and 1859674/5).
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bioRxiv - Cancer Biology 2019Quote: ... harvested after 4-5 days and counterstained with Near-IR fluorescent Dye (Live/Dead Cell Stain Kit, Invitrogen) to determine the number of viable dividing (CFSE/NearIR- ...
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bioRxiv - Systems Biology 2019Quote: ... The RNA was fragmented for 5 min at 95°C in the presence of Superscript III buffer (Invitrogen) followed by a five-minute incubation on ice ...
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bioRxiv - Cancer Biology 2020Quote: Cells were maintained at 37 °C under humidified 5% CO2 in DMEM medium supplemented with 10% FCS (Invitrogen) and 2 mM glutamine ...
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bioRxiv - Molecular Biology 2021Quote: ... At least 400 μl of purified erythrocytes were rapidly lysed in 5 volumes pre-warmed TRIzol (ThermoFisher Scientific) and stored at −80°C until further processing.
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were grown at 37°C with 5% CO2 and passaged using 0.05% Trypsin-EDTA solution (Thermo Fisher) or TryplE Express Enzyme (Thermo Fisher).
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bioRxiv - Neuroscience 2021Quote: ... cells were washed with PBS(−/−) three times and then blocking buffer (5% normal goat serum [Cat #PCN5000, ThermoFisher] ...
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bioRxiv - Systems Biology 2019Quote: ... followed by another centrifugation (1000 g, 5 min) and resuspension in 0.5 ml of PBS-BSA 0.01% + RiboLock (ThermoFisher) (0.8 U/µl) ...
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bioRxiv - Systems Biology 2019Quote: ... followed by another centrifugation (1000 g, 5 min) and resuspension in 0.5 ml of PBS-BSA 0.01% + RiboLock (ThermoFisher) (0.8 U/µl) ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was prepared by in vitro transcription using T7 RNA polymerase (5 U/μl final, Thermo Fisher Scientific) at 37 °C for 4 hours in the presence of 100 ng/μl digested plasmid ...
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bioRxiv - Molecular Biology 2021Quote: ... The coverslips were washed with PBS twice for 5 min each followed by staining with Hoechst 33342 (Invitrogen), for four minutes ...
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bioRxiv - Microbiology 2019Quote: ... The 5′ monophosphate and 3ʹ free –OH ends of the RNA were ligated with T4 RNA ligase (Invitrogen) to make circular molecules ...
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bioRxiv - Microbiology 2020Quote: ... were harvested in an Eppendorf tube and resuspended in 0.5 mL NaCl (0.85%) containing SYTO9 green-fluorescent nucleic acid stain (5 nM final concentration, Invitrogen). Following incubation in the dark for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were pooled (5 μl each) and the pooled products were cleaned using GeneJet PCR purification kit (ThermoFisher). Amplified barcodes were sequenced using an Illumina HiSeq 4000 with Illumina TruSeq primers ...
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bioRxiv - Pathology 2020Quote: ... OCT-embedded frozen specimens were cryosectioned at 5 μm onto charged slides (Fisher Scientific cat# 22-042-924). Slides were air-dried at room temperature overnight and fixed in 10% neutral buffered formalin for 1 hour at room temperature ...
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bioRxiv - Systems Biology 2020Quote: ... we spun down the cells at 1,000 g for 5 min and resuspended in quenching buffer (DBPS, Gibco, 0.1% BSA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Viral RNA was amplified on a Thermal Fisher QuantStudio™ 5 Real-Time PCR System (Thermo Fisher Scientific) using FastStart Universal SYBR Green Master mix (carboxy-X-rhodamine ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4 mM DTT and 20% glycerol) at 30 °C in a Cytation 5 imaging reader (Thermo Fisher Scientific) with filters for excitation at 360/40 nm and emission at 460/40 nm ...
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bioRxiv - Zoology 2020Quote: ... 2’-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-23491-52-3 (Hoechst 33342, trihydrochloride, trihydrate, Life Technologies, H3570) in water for 20 min ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 5 µg of total RNA using Superscript III Reverse Transcriptase (Invitrogen,Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were grown in 1:1 mix of Dulbecco’s Modified Eagle Medium (DMEM) and Ham’s F-12 growth media with 5% Fetal Bovine Serum (FBS) (Gibco) containing L-Glutamine and Phenol Red in 75 mm3 T-75 flasks at 37°C in 5% carbon dioxide (CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... was dissected out using #5 forceps and placed in a new dish containing ice-cold Hibernate A (Gibco) medium without calcium and magnesium ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 μL of purified RNA was added to a reaction mixture containing 1x First Strand buffer (Thermo Fisher), 5 mM dithiothreitol (DTT) ...
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bioRxiv - Microbiology 2021Quote: ... Probes consisting of oligodeoxyribonucleotides (see Supplementary Table 2) were 5′-end labeled using T4 polynucleotide kinase (ThermoFisher Scientific) with 25 μCi of [γ-32P]dATP ...
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bioRxiv - Immunology 2021Quote: ... Array were hybridized with 5 µg of labeled DNA and assayed on a GeneChip Scanner 3000 7G (Affymetrix). Raw data were generated and controlled with Expression console (Affymetrix ...
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bioRxiv - Immunology 2020Quote: ... supernatant was decanted and were then incubated for 5 minutes in RBC lysis buffer (Gibco ACK Lysing buffer) prior to final washing and in vitro assays or flow cytometry.
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bioRxiv - Molecular Biology 2020Quote: ... mESCs expressing mCherry:hCdt and Citrine:Geminin were cultured in 5% CO2 at 37 °C on 0.1% gelatin-coated dishes in DMEM KO (Gibco) media supplemented with 10% FCS ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were passaged 1:2-1:6 every 2-5 days by being rinsed once with DPBS (Gibco) and dissociated using 0.5 mM EDTA (75 μl/cm2 ...
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bioRxiv - Cell Biology 2019Quote: ... 500 ng of each probe were precipitated with 5 μl of Cot-1 DNA (1 mg/ml) (Invitrogen) and resuspended in 15 μl of hybridization buffer (10% dextran sulfate ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were incubated with the permeant sodium indicator sodium green tretraacetate (5 μM, #S6901, Invitrogen, Carlsbad, CA) in complete medium without phenol red ...
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bioRxiv - Cell Biology 2019Quote: ... Genes were quantified using SYBR Green following the manufacturer’s instructions on an QuantStudio 5 qPCR instrument (Applied Biosystems). Gene expression was normalized to an internal control (Rplp0 ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μg/mL gentamicin (Wisent) and 5 ng/mL recombinant mouse fibroblast growth factor basic (FGF-b, ThermoFisher). At ~90% confluence ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA was synthesized from 5 µg of RNA with random primers using Superscript II Kit (Invitrogen, Thermo Fisher). Real-time quantitative PCR was performed with Taqman probes from Applied Biosystems (Hs01568763_m1 for RAD51B ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA was synthesized from 5 µg of RNA with random primers using Superscript II Kit (Invitrogen, Thermo Fisher). Real-time quantitative PCR was performed with Taqman probes from Applied Biosystems (Hs01568763_m1 for RAD51B ...
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bioRxiv - Zoology 2019Quote: Total RNA was extracted from 2-5 larvae using a commercial kit (TRIZOL, Life Technologies, Carlsbad, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: A total of 5 µg RNA was treated with DNase using the TURBO DNA-free kit (Thermo Fisher) and then reverse-transcribed into cDNA using Superscript II or II (Thermo Fisher ...
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bioRxiv - Bioengineering 2020Quote: ... WT-PGP1 were labeled with 5 μM CellTracker™ Blue 7-amino-4-chloromethylcoumarin CMAC (Molecular Probes, #C2110), iNeuron-PGP1 was labeled with 2.5 μM CellTracker™ Green CMFDA (Molecular Probes ...