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Citations for Thermo Fisher :
7701 - 7750 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... They were cultured at 37 °C under 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM; Life Technologies, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... For qPCR reactions a QuantStudio 5 Real-Time PCR system and Fast SYBR Green Master Mix (Applied Biosystems) were used ...
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bioRxiv - Immunology 2020Quote: ... Amplification was carried out in a 7500 Real Time PCR machine or a QuantStudio 5 machine (Applied Biosystems). Reactions were performed in triplicate and all replicate values were within 0.5 cycles ...
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bioRxiv - Immunology 2021Quote: ... after which noninternalized bacteria were removed by four washes with PBS containing 5 μg/mL gentamycin (Life Technologies). Cells were further incubated at 37°C for either 20 min or 4 h ...
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bioRxiv - Immunology 2020Quote: ... pneumoniae was then spun down at 5000G for 5 minutes and resuspended in 1mL 1XPBS (Gibco, Gaithersburg, MD). Sub-cultured K ...
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bioRxiv - Immunology 2020Quote: ... Splenocytes resuspended at a density of 5×106 cells/ml in PBS had 1:5,000 CFSE (ThermoFisher Scientific) added and were incubated in the dark at room temperature ...
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bioRxiv - Immunology 2020Quote: ... 5% CO2 and medium were changed every 3 days and cells were passaged with 0,05% trypsin-EDTA (Invitrogen) twice per week.
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bioRxiv - Immunology 2020Quote: ... 2.5×107 cells/mL cells were subsequently labeled with 5 μM Cell Trace Violet (CTV) (Thermo Fisher Scientific) in warm PBS supplemented with 0.1% FCS for 20 minutes in the dark at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... The membrane was incubated for 5 min with the Lightning CDP Star Chemiluminescence reagent (ThermoFisher Scientific, Applied Biosystem) and exposed on CL-Xposure film (ThermoFisher Scientific).
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bioRxiv - Genomics 2020Quote: ... ribosomal RNA present was depleted from 5 μg of total RNA using the RiboMinus Eukaryote System (Life technologies). cDNA synthesis was performed using the SuperScript® III Reverse Transcriptase kit (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... 5-10µg of protein per sample was run on a 4%–12% NuPAGE Bis-Tris Gel (ThermoFisher Scientific) and then transferred to PVDF membrane by liquid transfer using NuPAGE Transfer buffer (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... The digested tissue was then pelleted and lysed at 47ºC for 5 minutes using ACK lysis buffer (Gibco). The pellet ...
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bioRxiv - Genomics 2019Quote: ... Cells were cultured at 37°C with 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2020Quote: ... Biotin labelled fragments which contained the 5’ and 3’ connecting region was enriched using Dynabeads M280 streptavidin (Invitrogen) and incubating at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptides were separated across a 2.1 m□×□5 cm C18 separating column (1.9 mm Hypersil Gold, Thermo Fisher) with a linear gradient of 4-40% CH3CN and 0.3% formic acid over 6 min ...
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bioRxiv - Neuroscience 2021Quote: ... 80 ± 5 mg of brain tissue samples were lysed in 1 mL of TRIzol™ Reagent (Thermo Fisher), according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 5% heat-inactivated fetal bovine serum (FBS) and 1X Penicillin-Streptomycin solution (Thermo Fisher Scientific, USA). SARS-CoV-2 clinical isolates (D614G variant ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell proliferation flow cytometry assays were performed using the 5-ethynyl-2’deoxyuridine (EdU) assay kit (Invitrogen #C10634). Cells were incubated with EdU for 24 h and labeled ...
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bioRxiv - Immunology 2022Quote: ... Mice were imaged 5 minutes after intraperitoneal injection with fresh d-luciferin (150 mg/kg; Thermo Fisher Scientific). Luciferase signal was quantified using Living Image software (Caliper LifeSciences ...
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bioRxiv - Microbiology 2022Quote: ... Vero-E6 cells and Calu-3 were treated with 5 μg/ml or 1 μg/ml puromycin (Gibco), respectively ...
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bioRxiv - Developmental Biology 2022Quote: ... qRT-PCR reactions were performed in real time using 5 µl PowerUp SYBR Green Master Mix (ThermoFisher, A25741), 0.8 µl of 10 µM forward and reverse primers ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were treated with 10 μM of Edu (5-ethynyl-2′-deoxyuridine; Invitrogen™, Thermo Fisher Scientific, USA), and incubated for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were treated with 10 μM of Edu (5-ethynyl-2′-deoxyuridine; Invitrogen™, Thermo Fisher Scientific, USA), and incubated for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then washed with PBS and incubated with streptavidin-fluorescein isothiocyanate (FITC; 5 µg/mL; Invitrogen) for 40 min ...
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bioRxiv - Microbiology 2022Quote: ... for 10 minutes and incubated in blocking buffer (2.8 mM KH2PO4, 7.2mM K2HPO4, 5% goat serum (Gibco #16210064), 5% glycerol ...
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bioRxiv - Microbiology 2022Quote: ... were obtained by collaborators and maintained at 37 °C and 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco), supplemented with 10% heat-inactivated fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2022Quote: ... for 5 minutes and mounted on glass slides (Star Frost) with Prolong Diamond Antifade Mountant (Life Technologies #P36970). Confocal microscopy was carried out with a Zeiss inverted LSM700 microscope ...
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bioRxiv - Cell Biology 2022Quote: WT organoids passage 10 were collected 5-7 days after passaging and digested with TrypLE (Thermo Fisher Scientific) for 20 min at 37 °C ...
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bioRxiv - Pathology 2022Quote: ... The secondary antibody (Alexa Fluor 594 goat anti-Mouse, ThermoFisher A11032, 1:1000 in 5% GS in PBS) was applied for 1 h at RT in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... 150mM NaCl supplemented with 5% BSA (w/v) and 0.1% NaN3(w/v)): Tim17a (Thermo Scientific, 1:1000), PRELID1 [aa27-54] (Sciquest ...
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bioRxiv - Microbiology 2022Quote: ... was processed in parallel 5 μg aliquots through the RiboMinus™ Transcriptome Isolation Kit (Invitrogen, Thermo Fisher Scientific) essentially as described in the manufacturer protocol with the exception that the magnetic beads were loaded with 3 μL of the pan-prokaryotic rRNA probe as well as 4 μL of the eukaryotic rRNA probe to deplete both host and chlamydial rRNA simultaneously ...
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bioRxiv - Microbiology 2022Quote: Human female cervical epithelial adenocarcinoma HeLa cells (RRID: CVCL_1276) were cultured at 37° C with 5% atmospheric CO2 in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... was processed in parallel 5 μg aliquots through the RiboMinus™ Transcriptome Isolation Kit (Invitrogen, Thermo Fisher Scientific) essentially as described in the manufacturer protocol with the exception that the magnetic beads were loaded with 3 μL of the pan-prokaryotic rRNA probe as well as 4 μL of the eukaryotic rRNA probe to deplete both host and chlamydial rRNA simultaneously ...
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bioRxiv - Biophysics 2022Quote: ... 5% tetramethylrhodamin-labeled microtubules were immobilized on the glass surface a 0.1 mg/mL anti-tetramethylrhodamin antibody (ThermoFisher). It should be noted that the microtubules were placed in parallel owing to the shear flow ...
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bioRxiv - Cell Biology 2022Quote: ... antibodies were 5-fold serially diluted starting from 100 μg/mL in DMEM medium supplementing 10% FBS (Gibco). Wells without antibodies or containing the same concentrations of REGN10933 were set as control ...
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bioRxiv - Cancer Biology 2022Quote: ... Supernatant was aspirated off and 500 μL of diluted (1:5) TrypLE Express (Cat no. 12604-013, Gibco) was added to each conical to dissociate cell pellet ...
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bioRxiv - Cell Biology 2022Quote: ... 5 μl of 175 nm orange and green nanobeads from a PS-Speck Point Source Kit (P7220, Invitrogen) are placed on a #1.5 cover slip and air dried ...
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bioRxiv - Developmental Biology 2022Quote: ... Filtrate was centrifuged and resuspended in stromal culture 5 mL medium containing 10% fetal bovine serum (FBS, Gibco) and 1X ITS-X (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were blocked for 1 hr at RT in 5% albumine bovine fraction V (Thermo Scientific, pH 7.0) washed in TBS + 0.05% Tween with pH 8.0 (TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... Cell nuclei were counterstained with 0.5 mg/mL DAPI for 5 min (Thermo Fisher Scientific, Rockford, IL, USA) and mounted using Vectashield Vibrance antifade mounting media (Molecular Probes ...
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bioRxiv - Cancer Biology 2022Quote: ... The reaction was stopped by adding cold blocking buffer (5 mM EDTA, 2% fetal bovine serum (FBS) (Gibco) in HBSS) ...
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bioRxiv - Genomics 2022Quote: ... The HEK293T cells were incubated in a humidified constant 37C and 5% CO2 incubator in DMEM medium (Gibco) with 100U/ml Penicillin-Streptomycin (Gibco ...
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bioRxiv - Genomics 2022Quote: ... the homogenate was boiled at 95°C for 5 min in NuPAGETM LDS Sample Buffer (Thermo Fisher Scientific) with NuPAGETM Sample Reducing Agent (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2022Quote: ... The lysis reaction was mixed with 2μl reverse transcription master mix 5×buffer (Thermo Fisher Scientific, Cat. 11756500), 2μl 5M Betaine (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: ... total placental RNA was extracted from approximately 5 mg of tissue using the “mirVana miRNA Isolation Kit” (Ambion) which efficiently isolates all RNAs longer than 10 nucleotides in length ...
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bioRxiv - Immunology 2022Quote: ... The cells were centrifuged at 400 g for 5 min at 4°C and resuspended in DPBS (GIBCO) supplemented with 5% FBS ...
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bioRxiv - Genomics 2022Quote: ... cells were resuspended in 5 ml N2B27 medium (50/50 mix of DMEM-F12 (Thermo Fisher Scientific, 11320033) and Neurobasal (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... TIL were stained before seeding at day 11 of the REP with 5 μM CFSE (Thermo Fisher Scientific) for 20 min at 37°C ...
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bioRxiv - Genomics 2022Quote: ... HEK-293T cells (ATCC CRL-3216) were maintained at 37°C and 5% CO2 in DMEM (ThermoFisher 11995073) with 10% FBS (Corning 35015CV) ...
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bioRxiv - Genomics 2022Quote: NIH-3T3 cells (ATCC CRL-1658) were maintained at 37°C and 5% CO2 in DMEM (ThermoFisher 11995073) with 10% cosmic calf serum (Cytiva SH30087.03) ...