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Citations for Thermo Fisher :
7801 - 7850 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... T cells were stimulated for 5 days with Dynabeads Human T-Activator CD3/CD28 (Life Technologies, Carlsbad, CA) at a bead-to-cell ratio of 3:1 ...
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bioRxiv - Biophysics 2024Quote: ... which is the same medium as above with 5 μg·ml-1 hygromycin B (Thermo Scientific Chemicals, J60681-MC) replacing the zeocin.
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bioRxiv - Cell Biology 2024Quote: ... Cells were subcultured at a ratio of 1:10 every 5–6 days using 0.5 mM EDTA (Invitrogen) to detach clumped cells.
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bioRxiv - Cell Biology 2024Quote: ... SEM images were acquired on a Helios 5 UX DualBeam FIB-SEM platform (Fisher Scientific, Brno, Czech Republic) using SEM imaging mode at 5 kV and 0.1 nA using TLD and ICD detectors to image secondary electron and backscatter electron signal ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with PBS and incubated 5 minutes with PBS containing 2 µg/mL Hoechst (33342, Fisher Scientific, UK) and washed in PBS and briefly in ddH2O then mounted on glass slides using ProLong GoldTM anti-fade glass mounting medium (P36980 ...
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bioRxiv - Microbiology 2024Quote: Human cervical carcinoma cell line HEp2 cells (ATCC® CCL-23) were cultured at 37°C with 5% atmospheric CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were subjected to a 5-minute wash with buffer (0.35% Triton-X, Thermo Fisher PRH5142, in PBS) and permeabilized with 0.5% Triton-X in PBS for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... Huh7 cells were cultured in the same incubator conditions (37 °C, 5% CO2) in low glucose DMEM (Gibco) media supplemented with 10% FBS (Gibco 26140095) ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293T cells were cultured in a controlled humidified incubator at 37 °C and 5% CO2 in DMEM (Gibco) media supplemented with 10% FBS (Gibco 26140095) ...
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bioRxiv - Molecular Biology 2024Quote: ... The washed membrane was then incubated overnight at 5°C with a 6x-His-Tag monoclonal antibody (Invitrogen) diluted 2500-fold in PBS-Tween ...
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bioRxiv - Immunology 2024Quote: ... or BA.5 (2 μg/mL) spike proteins were coated onto 96-well Maxisorp plates (ThermoFisher, Cat. #439454) in 50 μL of 50 mM Na2CO3 pH 9.6 overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Propidium iodide (PI) and the pH sensitive 2’,7’-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein (BCECF) (Invitrogen) were added to these at a final concentration of 100µM and 10µM respectively ...
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bioRxiv - Neuroscience 2024Quote: ... each animal was injected intraperitoneally with 37 mg/kg BW of 5-ethynyl-2′-deoxyuridine (EdU, ThermoFisher, A10044) at 48 hours after surgery ...
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bioRxiv - Plant Biology 2024Quote: ... The sections were cut to a thickness of 5 μm using a Manual Rotary Microtome (Thermo Fisher Scientific). Sections were incubated with the primary anti-FtsZ2-1 antibody (1:3500 dilution) ...
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bioRxiv - Neuroscience 2024Quote: ... each animal was injected intraperitoneally with 37 mg/kg BW of 5-ethynyl-2′-deoxyuridine (EdU, ThermoFisher, A10044) at 48 hours after surgery ...
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bioRxiv - Molecular Biology 2024Quote: ... 5-30 µg of lysates were resolved on pre-cast 10% or 12% Bis-Tris NuPAGE gels (Invitrogen), transferred to PVDF membranes (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... perforatum and 5 μg was transcribed into cDNA using the SuperScriptTM IV First-Strand Synthesis system (ThermoFisher, Invitrogen). Candidate gene ORFs were PCR-amplified (Table S17 ...
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bioRxiv - Plant Biology 2024Quote: ... perforatum and 5 μg was transcribed into cDNA using the SuperScriptTM IV First-Strand Synthesis system (ThermoFisher, Invitrogen). Candidate gene ORFs were PCR-amplified (Table S17 ...
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bioRxiv - Neuroscience 2023Quote: ... The reaction was stopped by adding 5 milliliters of HBSS lacking Ca2+and Mg2+ (Gibco, catalog no. 14175095). The dissociated neuron resuspension was then centrifuged for four minutes at 200 rcf ...
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bioRxiv - Neuroscience 2024Quote: ... with 10 ng cDNA input in triplicates on a QuantStudio 5 real-time PCR system (Thermo Fisher Scientific). Primer sequences are listed in Supplemental Table S1 ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa and COS-7 cells were seeded onto dishes coated with 5 mg fibronectin (Life Technologies 33016-015) in diH2O ...
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bioRxiv - Developmental Biology 2024Quote: ... pelleted by centrifugation and resuspended in 5 ml DMEM + 10% FBS + Pen/Strep/Glutamine (Invitrogen Cat# 10378-016). Cells were plated to achieve the greatest density possible depending on the number of cells available ...
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bioRxiv - Immunology 2024Quote: ... Isolated BM cells were maintained at 37°C and 5% CO2 in DMEM (4.5 g/L glucose, Gibco) supplemented with 10% heat-inactivated fetal calf serum (FCS) ...
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bioRxiv - Immunology 2024Quote: ... after which the cells were resuspended into 1-5 mL FBS and counted on a Countess (ThermoFisher Scientific) with trypan blue ...
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bioRxiv - Cell Biology 2024Quote: ... The coverslip with stained cells was mounted with 5 µL of ProLong Glass antifade mount (Thermo Fisher Scientific) on a slide glass ...
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bioRxiv - Cell Biology 2024Quote: ... SEM images were acquired on a FIB-SEM platform (Helios 5 UX DualBeam Fisher Scientific, Brno, Czech Republic) using SEM imaging mode at 1.8 kV and 0.1 nA using TLD detector.
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bioRxiv - Microbiology 2024Quote: ... exponentially growing cells in suspension were diluted to 3×109 and 5 µl spotted on 1.5% agar (Gibco) supplemented with 0.5% CTT and immediately covered with a coverslide ...
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bioRxiv - Microbiology 2024Quote: ... 5 μL of this solution was introduced into the Ultimate 3000 RSLCnano UHPLC system (Thermo Scientific, Germeringen, Germany). Peptide separation was performed on a 75 μm × 250 mm analytical column (C18 ...
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bioRxiv - Immunology 2024Quote: ... T cells were stained before the start of the assay with CellTraceViolet (CTV) (Invitrogen, 5 µM in PBS0) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Processed cells were centrifuged at 1200 rpm for 5 min and resuspended in ACK lysing buffer (Gibco, A10492) for 20 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... 5 x 106 to 1 x 107 cells per sample were lysed by RIPA buffer (Thermo Fisher, 89900) and shaken on a rocker for 1 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 1 ug of RNA isolated from JE2 WT was subjected to reverse transcription by using 5՚-phosphorylated primer pepV_TSS_R1 and the first strand cDNA synthesis kit (Invitrogen, Superscript III First-Strand Synthesis System) ...
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bioRxiv - Genomics 2024Quote: ... which included 5 µL of 4x TaqPathTM 1-step RT-qPCR Master Mix (Thermo Fisher Scientific, MD, USA), 1 µL of 10 µM forward primer ...
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bioRxiv - Immunology 2024Quote: ... Transcript levels were determined by real-time quantitative polymerase chain reaction (QuantStudio 5; Thermo Fisher Scientific, Waltham, MA), normalized to the control gene Abelson (ABL ...
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bioRxiv - Immunology 2024Quote: ... in virus growth medium (1X MEM [Gibco 11095080], 5% FBS [Hyclone SH30070.03HI] and 1% Penn-Strep [Gibco 10378016]). Monoclonal antibody dilution plates were prepared similarly to serum dilution plates ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Coronal sections of brain tissue (5 μm) were made using a Shandon Finesse 325 microtome (Thermo Fisher Scientific). Brain slices were stained with hematoxylin (Merck ...
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bioRxiv - Biochemistry 2024Quote: ... TMPRSS2+ Vero E6 cells were cultured at 37°C in the presence of 5% CO2 in medium composed of in high-glucose Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Biophysics 2024Quote: ... The reaction was stopped by adding 5 ul of 4X NuPage LDS-loading dye (Thermo Scientific cat #NP0008) supplemented with 5 % 2- mercaptoethanol ...
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bioRxiv - Biochemistry 2023Quote: ... temperature-dependent protein unfolding profiles were measured using the QuantStudio™ 5 realtime PCR machine (Thermo Fisher Scientific). Excitation and emission filters were set to 465 nm and 590 nm ...
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bioRxiv - Biochemistry 2023Quote: ... for 5 min followed by destaining with Tris-buffered saline-0.05% Tween 20 (TBST) (Thermo Scientific, J77500.K2). The membranes were blocked for 1 h at RT with 5% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2023Quote: ... cells were cultured at 37 °C and 5% CO2 in Dulbecco’s modified Eagle’s medium with glutamine (DMEM) (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Genomics 2023Quote: ... Real-time PCR was performed using a QuantStudio 5 Real-Time PCR System (Applied Biosystems, Foster City, CA). The PCR cycling conditions were as follows ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were thawed at room temperature and washed three times for 5 minutes in DPBS (Gibco, 14190–094). Sections were then counterstained for 5 minutes with Hoechst 33342 (1μg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... Peritoneal Lavage cells were centrifuged at 1200 rpm for 5 min and resuspended in alpha MEM (Gibco; 12571063) media supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... A final wash in PBS for 5 mins was carried out before mounting in ProLong Gold (Invitrogen, P36930). Slides were stored at −20°C until imaged.
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: ... then washed 3x for 5-10min with PBST and mounted in ProLong Antifade Mounting medium (Thermo Fisher Scientific).
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bioRxiv - Genetics 2023Quote: ... Cells were then blocked with 2 mL blocking solution made of 5% Normal Goat Serum (cat#PCN5000 Thermofisher) and 0.05% Triton X-100 in PBS for 2 hours at RT or 4°C on a shaker.
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bioRxiv - Biophysics 2023Quote: ... and the fluorescence signals were traced by the quantitative real-time PCR QuantStudio 5 (Thermo Fisher Scientific, USA) with λex = 495 nm and λem = 520 nm ...
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bioRxiv - Microbiology 2023Quote: ... were prepared by dissolving DCF-DA at a final concentration of 5 mM in absolute ethanol (Fisher Scientific) and stored at – 20 °C ...
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bioRxiv - Microbiology 2023Quote: ... were cultured at 37 °C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, WAKO) containing 10% fetal bovine serum (FBS) (Gibco) and penicillin/streptomycin (100 U/mL ...