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Citations for Thermo Fisher :
7751 - 7800 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Genes encoding the Omicron (BA.5) Spike was synthesized and cloned by GeneArt (Thermo Fisher Scientific, Waltham, MA) in the pcDNA3.4 expression vector ...
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bioRxiv - Developmental Biology 2022Quote: ... media was removed from each plate and cells were washed with 5 mL of DPBS (Gibco, 14190-094). 3 mL of 0.25% Trypsin-EDTA (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... containing 5 mL of digestion buffer [composed of 9 mL of phosphate-buffered saline (PBS; Gibco, 10010-023) combined with 1 mL of Dispase (stock ...
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bioRxiv - Immunology 2022Quote: ... PCR was performed in a QuantStudio 5 real time thermal cycler for 35 cycles (Applied Biosystems, Waltham, MA).
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bioRxiv - Plant Biology 2022Quote: One μg of total RNAs extracted from 5-day-old seedlings using TRI-ReagentTM solution (Thermo Fisher Scientific) was subjected to RNA gel blot analyses ...
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bioRxiv - Microbiology 2022Quote: ... The RT-PCR reaction (with 5 μL template viral RNA) was performed using the QuantStudio (Thermo Fisher Scientific) according to instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... Tilt-series were collected with a dose-symmetric tilt scheme using TEM Tomography 5 software (Thermo Fisher Scientific). The tilt span of ± 60° was used with 3° steps starting at either ± 10° to compensate for the lamella pre-tilt ...
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bioRxiv - Physiology 2022Quote: ... a small amount (∼ 5 nl) of 0.2% FluoSpheres (0.1 µm diameter, 0.2% solids in saline; F8801 or F8803, Thermo Fisher) was injected at the same site with the same pipette at the end of recordings.
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation for 1 h in blocking solution containing 5% goat serum (16210–064, Thermo Fisher Scientific), 3% bovine serum albumin (BAC62 ...
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bioRxiv - Microbiology 2022Quote: ... coverslips were rinsed in deionized water and mounted onto slides with 5 μL of Prolong Diamond (Thermo Fisher). The mountant was cured for 45 min at 37°C or overnight at room temperature before imaging ...
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bioRxiv - Microbiology 2022Quote: ... CD4(+) T cells were stimulated with 5 μg/mL phytohemagglutinin (PHA; MiliporeSigma) in complete RPMI-1640 (Thermo Fisher) supplemented with 10 mM HEPES ...
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bioRxiv - Microbiology 2022Quote: ... BIECs were cultured at 37°C and 5% CO2 in DMEM/F12 (Gibco, Thermo Fisher Scientific, Waltham, MA) supplemented with 5% FBS ...
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bioRxiv - Microbiology 2022Quote: ... BIECs were cultured at 37°C and 5% CO2 in DMEM/F12 (Gibco, Thermo Fisher Scientific, Waltham, MA) supplemented with 5% FBS ...
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bioRxiv - Microbiology 2022Quote: ... 10 mM KCl) and then supplemented with 5% (v/v) B-PER Bacterial Protein Extraction Reagent (Thermo Scientific) before sonication ...
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bioRxiv - Immunology 2022Quote: ... cells were stained for 1 hour at 37°C/5% CO2 with 1μM CellTracker Deep Red (Invitrogen, C34565) and 1 drop/mL NucBlue Live ReadyProbes Reagent (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... All cells were cultured at 37°C and 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) with high glucose (4.5 g/L) (Gibco 11965118) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% fetal calf serum (Capricorn scientific, Germany) and 0.1% penicillin-streptomycin (Thermo Fisher Scientific, Bleiswijk, Netherlands) at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... The qRT-PCR mixture contains 5 ul TaqMan™ Universal Master Mix II no UNG (Applied Biosystems, #4440040), 0.9 ul 10 uM primer mix (F ...
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bioRxiv - Molecular Biology 2023Quote: ... to which 5 µL of Turbo DNase 10X Buffer and 1µL of Turbo DNAse (Thermo Scientific, cat#AM2238) were added ...
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bioRxiv - Neuroscience 2022Quote: ... Triturated tissue was then digested for 5 min using the recombinant protease TrypLE Express (ThermoFisher Scientific, cat. #12605) at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... qPCR was performed in 384-well plates on an Applied Biosystems QuantStudio 5 qPCR-System (Thermo Fisher Scientific) using PowerUp SYBR Green master mix (A25743 ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with PBS, BSA (0.5%), incubated (5 min, RT) with a viability dye (Aqua-dye, Invitrogen) and surface stained with titrated amounts of antibodies for 30 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were boiled for 5 minutes then electrophoresed in pre-cast 4-12% NuPAGE Bis-Tris Gels (Invitrogen). Following transfer ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue sections were blocked for 30 min in a blocking buffer (containing 5% horse serum (Thermo Fisher Scientific) and 1% Triton X-100 in PBS) ...
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bioRxiv - Microbiology 2022Quote: ... the RACE adapter (see Table S2) was ligated to 1-5 µg RNA using T4 RNA ligase (Invitrogen) for 1 hour at 37 °C or 2 hours at 25 °C ...
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bioRxiv - Microbiology 2022Quote: ... Goat anti-Mouse IgG conjugated with Alexa Fluor™ Plus 488 (5 μg/mL final concentration, Invitrogen A32723), and 10 μM Hoechst 33342 for 120 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Infection was observed after 5 days by measuring GFP fluorescence with a Cellomics ArrayScan HCS reader (Thermo Scientific). The percentage of GFP+ cells was calculated using Cellomics Scan software with identical settings for all wells analysed in a single experiment ...
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bioRxiv - Molecular Biology 2022Quote: Cells obtained after enzymatic digestion of testis were resuspended in 5-10 ml FACS buffer (1x DPBS [Gibco] supplemented with 1% Pen-Strep ...
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bioRxiv - Cell Biology 2022Quote: ... The TAP was removed and replaced with 5 mL of Max Efficiency Transformation Reagent for Algae (Invitrogen, 100021485). This was repeated 2 times ...
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bioRxiv - Microbiology 2022Quote: ... and BSR T7/5 cells (RRID:CVCL_RW96) were grown in in Dulbecco’s modified Eagle’s medium (DMEM, ThermoFisher Scientific, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: Hela cells were seeded in 6-well plates with cover slips and grown to 50% confluency at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (12430047, Invitrogen) supplemented with 10% heat-inactivated FBS (A4766801 ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% heat-inactivated fetal bovine serum (FBS, Cytiva #SH30071.03) and 1X Antibiotic-Antimycotic (Gibco #15240-062). VeroE6/TMPRSS2 (JCRB #1819 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Sections were washed 3 times 5 minutes in PBS and mounted using ProLong Gold mouting media (Thermo Fisher) under glass cover slips.
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bioRxiv - Molecular Biology 2022Quote: ... pH 7.3) was added in a buffer exchange and 5 μM Fura-4F AM (catalog number F14175, Invitrogen) was added to the myofibers for 30 minutes at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... centrifuged at 500xg for 5 min at 4°C and resuspended in 1% BSA in PBS (AM2616, Invitrogen) containing 200 U/μl RNase inhibitor (3335402001 ...
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bioRxiv - Developmental Biology 2022Quote: ... A total of 5 μg DNA was immunoprecipitated with preblocked protein A/G Dynabeads (Thermo Fisher Scientific, MA) complexed to 2.5 μg antibody (anti-CTCF ...
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bioRxiv - Cell Biology 2022Quote: ... Isolated BM cells were maintained at 37 °C and 5% CO2 in DMEM (4.5 g/L glucose, Gibco) supplemented with 10% heat- inactivated fetal calf serum (FCS) ...
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bioRxiv - Neuroscience 2023Quote: ... Capillaries were filled with 5-10 μL of dye solution of 10 μg/μL DiD (Thermo Fisher Scientific) in 100% ethanol and the tip was approached to the bristle socket with a micromanipulator ...
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bioRxiv - Cell Biology 2024Quote: ... Minced tissues were digested at 37°C in an enzymatic digest buffer containing 5 mg/mL Dispase (Invitrogen), 2 mg/mL DNase I (STEMCELL Technologies) ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% fetal bovine serum (FBS) (Cytiva, SH30396-03) and 5% penicillin-streptomycin (pen-strep) (Gibco, 15140163) or DMEM with 4.5 g/L D-glucose ...
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bioRxiv - Cell Biology 2024Quote: ... PI (propidium iodide) uptake was measured after 5 min incubation with 100 μg/mL diluted PI solution (ThermoFisher) in complete medium at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... The probe shank was coated before recordings by dipping 10 times every 5 s in DiI (V22885, ThermoFisher) for post hoc histological assessment of electrode tracks ...
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bioRxiv - Molecular Biology 2023Quote: ... The mobile phase consisted of buffer A: 5 mM ammonium acetate in water (LC/MS grade, Thermo Scientific) and buffer B ...
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bioRxiv - Biophysics 2024Quote: HEK293 cells were maintained at 37 °C in 5% CO2 containing Dulbecco’s modified Eagle’s medium (DMEM, purchased from Thermofisher) supplemented with 10% fetal bovine serum and penicillinstreptomycin (0.2 mg/ml) ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were further lysed via sonication (10 sec on 5 sec off, repeat 3x, ThermoFisher: FB120, 4°C) and protein was clarified by centrifugation at 21,000 x g for 5 min at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... 15 μL of protein sample was supplemented with 5 μL of 4X NuPAGE LDS Sample Buffer (Thermo Scientific). Samples were incubated at 95°C for 10 minutes before loading on a 4-12% SurePAGE™ Bis-Tris precast gels (Witec AG) ...
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bioRxiv - Biochemistry 2024Quote: ... Off-line fractionated samples (5 μl aliquot of 15 μl sample) were injected via an autosampler (Thermo Scientific) onto a 5 μl sample loop which was subsequently eluted onto an Acclaim PepMap 100 C18 HPLC column (75 μm x 50 cm ...
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bioRxiv - Biochemistry 2024Quote: ... the cells (5×105 cells per well) were incubated in six-well plates (Thermo Fisher Scientific, Nunclon Delta) for 2 h before stimulation with 100 ng/mL lipopolysaccharide (from E ...
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bioRxiv - Cancer Biology 2024Quote: ... then lysed with 1ml of polysomal buffer (20 mM Tris, pH 7.4, 150 mM NaCl, 1.25 mM MgCl2, 5 U/ml RNase inhibitor [Invitrogen] ...
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bioRxiv - Cancer Biology 2024Quote: ... Coverslips were washed 5 times in PBS and mounted with ProLong Gold Antifade Mountant with DAPI (Invitrogen, P36931) and imaged on the VS200 or an Echo Revolve microscope.