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Citations for Thermo Fisher :
7451 - 7500 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were blocked for one hour at RT in buffer containing 5% normal goat serum (NGS) (ThermoFisher: PCN5000) and 0.3% Triton-X in 1x PBS and incubated in primary antibodies in antibody dilution buffer (1% BSA and 0.3% Triton-X in 1x PBS ...
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bioRxiv - Immunology 2020Quote: ... Tissue sections were then stained with DAPI (Thermo Fisher Scientific, USA, 1:5000, 5 min at 37°C) with subsequent mounting with Prolong Diamond Antifade mountant (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... The lysis was quenched after 15 min with 5 ml of complete Hibernate A (Thermo Fisher Scientific, A1247501) supplemented with B27 ...
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bioRxiv - Neuroscience 2021Quote: ... we injected the fluorophore BODIPY TMR-X into MOs (Invitrogen; 5 mM in PBS 0.1 M, DMSO 40%). After 3 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then spun down at 200g for 5 minutes and resuspended in 100 μL Trizol (Life Technologies). For RNA extraction from tissue ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed 4 × 5 min with PBS before incubating with secondary antibodies (1:1000 Alexa fluorophores, ThermoFisher) in blocking solution for 1 hr at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... 50 mM MgCl2.) Aliquots (5 µL) were taken and S1 nuclease (0 to 1000 U; Thermo Fisher Scientific) was then added and the incubation continued in S1 nuclease buffer (30 mM sodium acetate pH 4.6 ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Reverse 5′-CGA AGG TGT GAC TTC CAT G-3′) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was established in parallel using purified SARS-CoV-2 viral RNA.
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bioRxiv - Cancer Biology 2020Quote: ... as per the commercial protocol) and 5 μL of fluorescent tag kit (Alexa Fluor® succinimidyl esters, Invitrogen, Molecular Probes® ...
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bioRxiv - Cancer Biology 2020Quote: ... were cultured in 5% CO2 at 37°C with humidity in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, Invitrogen) supplemented with 4 mM L-glutamine ...
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bioRxiv - Cancer Biology 2020Quote: ... were cultured in 5% CO2 at 37°C with humidity in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, Invitrogen) supplemented with 4 mM L-glutamine ...
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bioRxiv - Cancer Biology 2019Quote: ... and incubated at 37°C in an humified 5% CO2 atmosphere.All the cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Life Technologies) supplemented with 10% FBS (Boehringer Mannheim) ...
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bioRxiv - Cell Biology 2021Quote: Samples of RNA from SKM-1 and MS-5 co-cultures were collected and extracted using Trizol (Invitrogen) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections from 3- to 5-month-old mice were exposed to 2.5 µM MitoSOX Red (Molecular Probes), at 37°C for 30 min14 ...
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bioRxiv - Neuroscience 2019Quote: HEK293T cells (ATCC cell line CRL-3216) were cultured at 37°C in 5% CO2 in DMEM (GIBCO) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2019Quote: ... Odorants were used as pure or diluted in mineral oil (CAS 8042-47-5, cat.num. 124020010, Acros Organics, Thermo Fisher Scientific ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... Infected cell pellets were centrifuged at 5000xg for 5 min at 4 °C and resuspended in Trizol (Ambion).
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bioRxiv - Biochemistry 2020Quote: ... 400 μl of the solubilized chromatin was pre-incubated with 5 μl Anti-Myc antibody (PA1-981; Invitrogen) for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... or 5 µl of EZ-Link™ Sulfo-NHS-Biotin (21217, Thermo Fisher Scientific, 1 µg/20 µl), while still attached via the umbilical cord to the mother’s blood circulation ...
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bioRxiv - Plant Biology 2020Quote: ... The solution was sonicated for 3-5 mins using an ultrasonic cleaner (FS220, Thermo Fisher Scientific, Waltham, MA). The solution was diluted to 10 mL with 2% nitric acid ...
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bioRxiv - Plant Biology 2020Quote: ... The UHPLC systems was equipped with a C18-precolumn (Ø: 0.3 mm, 5 mm; PepMap, Thermo Fisher Scientific) and an Acclaim® PepMap analytical column (ID ...
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bioRxiv - Microbiology 2020Quote: ... All cells were cultured at 37°C and 5% CO2 in Dulbecco’s modified Eagle medium (DMEM, Life Technologies) containing 10% fetal bovine serum ...
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bioRxiv - Neuroscience 2021Quote: ... 5-10μm coronal sections containing the ventral hippocampus were collected on Super Frost Ultra Plus slides (Fisher Scientific) and were processed for either fluorescent in-situ hybridization (FISH ...
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bioRxiv - Microbiology 2021Quote: ... Amoebae were washed and resuspended in M199s medium and labeled with CellTracker green 5-chloromethylfluorescein diacetate (CMFDA; Invitrogen) at 186 ng/ml for 10 minutes at 35°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... + 5% BCS + 0.4 ug/ml hydrocortisone + 10 ng/ml epidermal growth factor (EGF) + 1% Penicillin-Streptomycin (ThermoFisher Scientific). After two days incubation ...
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bioRxiv - Developmental Biology 2020Quote: mESC-derived LPM cells were transfected at day 5 in 150µl of Opti-MEM (Thermo Fisher Scientific, 31985088) with 2µl of Lipofectamine Stem (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: 5 µL of RT-qPCR reaction was digested with 1 µL FastDigest PstI restriction enzyme (#FD0614, ThermoFisher Scientific) for 1 h at 37°C ...
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bioRxiv - Microbiology 2021Quote: POWV-LI9 (MOI, 5) was adsorbed to 80% confluent VeroE6 monolayers cultured in Lab-Tek II chambers (Nunc), washed with PBS ...
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bioRxiv - Neuroscience 2020Quote: ... 14 DIV cultures were incubated with the lipophilic dye CM-DiI (ThermoFisher C7000, 5 µg/ml, 20 min), followed by a PBS wash ...
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bioRxiv - Neuroscience 2019Quote: ... Cortical astrocytes were grown in DMEM/F12 medium supplemented with 10% fetal bovine serum and 5% of 10,000 units/ ml of penicillin and 10,0000 µg/ml of streptomycin (GIBCO) at 5% CO2 and 37 °C.
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bioRxiv - Microbiology 2021Quote: ... the monolayers were overlaid with 2 ml of Minimal Essential Medium (Mediatech) supplemented with 5% FBS (Life Technologies), nonessential amino acids (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... qPCR reactions of 10 µL were set up with 5 µL PowerUpTM SYBRTM Green Master Mix (Applied Biosystems), 500 nM of each primer OT5/OT and OT7/OT8 and 20 ng of DNA ...
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bioRxiv - Microbiology 2021Quote: ... The gel was washed twice with water for 5 min followed by incubation with renaturation buffer (ThermoFisher, #LC2670) for 21 h at 30° C ...
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bioRxiv - Microbiology 2021Quote: ... 10 ng of cDNA was used as template in the QuantStudio 5 Real-Time PCR system (Applied Biosystems). Amplifications were performed with 5 μl of SYBR® green JumpStart Taq ReadyMix (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... falciparum parasites (NF54 strain from MR4, Manassas, VA) were maintained in 5 mL RPMI 1640 medium (Life Technologies) supplemented with 10% heat-inactivated (56 °C for 45 min ...
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bioRxiv - Cancer Biology 2021Quote: ... TNBC1 spheres were dissociated into single cells by trypsinization for 5 min with 0.05% trypsin (Gibco, #25300-62). 1X trypsin inhibitor (1000X ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were cultured at 37C and 5% CO2 either in 1:1 mix of DMEM GlutaMAX (Gibco, 31966047) and F-12 media (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... 5 to 20 ng of RNA were amplified and labelled with the GeneChip 3’ IVT Pico Kit (Affymetrix) according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µg of GST-fused bait were coupled to Pierce Glutathione Magnetic Agarose Beads (Thermo Fisher Scientific, #78602), previously washed twice with equilibration buffer (Tris-HCl 125 mM ...
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bioRxiv - Cell Biology 2020Quote: ... samples were boiled for 5 min before analysis by SDS-PAGE gels stained with SimplyBlue Safe Stain (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... Samples were boiled 5 min at 95 °C and loaded in pre-cast Novex Tris-Glycine gels (Invitrogen). Pre-cast gels were run in an XCell SureLock™ Mini-Cell Electrophoresis tank (ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: NO production was detected by DAF-FM diacetate (4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate) (D23844, ThermoFisher). Fly larvae were dissected at 24 or 48 h AI in PBS to expose the sensory neurons ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were boiled for 5 minutes at 95°C in NuPAGE™ LDS Sample Buffer 4X (Thermo Fisher) + 5% BME (Sigma-Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... HAP1 cells were grown at 37 °C with 5% CO2 in Iscove’s Modified Dulbecco’s medium (IMDM) (Gibco, #sh30228) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then stained with 5 µL FITC anti-human DR4 (Thermo Fisher Scientific, Clone DR-4-02) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... and cultured at 35°C with 5% CO2 in medium containing 7.4 g/L MEM (ThermoFisher #11700-077) with the following supplements (in mM unless labeled otherwise) ...
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bioRxiv - Cell Biology 2020Quote: ... Dy-Light 488-conjugated rat anti-mouse GPIbβ Ab (0.15µg/g;Emfret) and Alexa 647-conjugated fibrinogen (5% total fibrinogen; Invitrogen) were administered via a cannula inserted in the jugular vein ...
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bioRxiv - Cell Biology 2021Quote: ... Isolated islets were cultured at 37°C and 5% CO2 in RPMI 1640 media (Thermo Fisher Scientific, #11879020) containing 8 mM glucose ...
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bioRxiv - Cell Biology 2021Quote: ... incubated for 3 to 5 min in 100 μM Lysotracker-Red DND-99 (Life Technologies, Carlsbad, CA, USA)[49] and 1 μM Höchst 33342 (Thermo Fisher Scientific Inc ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated at a final concentration of 5 μM CellROX® Orange reagent (Life Technologies, Carlsbad, USA) for 30 min at 37 °C ...