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Citations for Thermo Fisher :
7551 - 7600 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... followed by an incubation of 100 μl of a 2:1 (A:G) mixture of Dynabeads Protein A and G (Invitrogen, 10001D and 10004D) for 2h at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... The 2° antibody used was Alexa Fluor® 488 Goat antiMouse IgG1 Cross-Adsorbed Secondary Antibody (1:100,ThermoFisher Scientific, Cat. A21121)
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bioRxiv - Cancer Biology 2022Quote: ... PaCaDD-165 and MaPaC-107 were cultured in a 2:1 mixture of supplemented DMEM as described above and Keratinocyte-Serum-free medium (KSFM, Thermo Fisher Scientific) supplemented with 2.5 μg Epidermal Growth Factor (EGF ...
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bioRxiv - Cell Biology 2022Quote: Sample #2 contained 2.5 μL of a 1:5000 dilution of both 100 nm diameter green microspheres (505/515, ThermoFisher, Cat# F-8803) and 1 µm orange microspheres (540/560 ...
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bioRxiv - Cell Biology 2022Quote: ... in blocking buffer and then for 2 hours at room temperature with Alexa Fluor–conjugated secondary antibodies (1: 1000, Thermo Fisher Scientific) in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... the fish were paralyzed before being mounted in agarose by bathing them for 2-5 min in a solution of 1 mg/mL α-bungarotoxin (Thermofisher Scientific) in E3 medium ...
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bioRxiv - Genetics 2023Quote: ... cells were stimulated with Dynabeads Human T-Activator αCD3/αCD28 at a bead-to-cell ratio of 1:2 (11131D, Thermo Fisher Scientific). Cell counts were determined every 2-3 days ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted with equal volume of phenol:chloroform:isoamyl alcohol (25:24:1) and the aqueous layer was transferred to new tube containing NaCl (final = 200 mM) and 2 µL GlycoBlueTM (ThermoFisher Scientific, AM9515). 750 µL of ice-cold 100% ethanol was added ...
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bioRxiv - Immunology 2023Quote: ... peptides were resuspended in 0.1 % TFA and loaded on 50 cm Easy Spray PepMap column (75 µm inner diameter, 2 µm particle size, Thermo Fisher Scientific) equipped with an integrated electrospray emitter ...
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bioRxiv - Molecular Biology 2023Quote: ... the brain sections were washed for 5 min x3 in 0.1% Tween 20/PBS and incubated in fluorescence tagged secondary antibodies for 2 hours at RT: Alexa Fluor 594 anti-rabbit (1:500, ThermoFisher Scientific, A11037), Alexa Fluor 488 anti-mouse IgG1 (1:500 ...
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bioRxiv - Physiology 2023Quote: ... and incubated for a period of 1 h in a dark room in 2 ml of extracellular solution containing 5 µM fluo- 4 AM (Thermo Fisher Scientific) or 7 µM Corona Green AM (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were then immunolabeled with Alexa Fluor 647-conjugated donkey anti-mouse IgG (Yeasen, Shanghai) in PBS with 1:400 dilution for 2 h and the nuclear counterstain DAPI (Thermo Fisher Scientific) for 5 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... The medium was then replaced with a fresh solution of 1 mM 2-NBDG and 350 nm TMRM (T668, Thermo Fisher Scientific) in L-15 and incubated for a further hour at room temperature ...
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bioRxiv - Genomics 2023Quote: ... When hiPSCs were visually estimated to be 80% confluent they were passaged 1-2 times using Dispase II (2mg/ml; Gibco/Life technologies) in mTeSR1 and plated onto Matrigel coated plates ...
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bioRxiv - Neuroscience 2023Quote: ... sections were blocked (2% Normal Goat serum, 0.3% Triton X-100 in PBS) and incubated with anti-GFP (chicken, Invitrogen, A10262, 1:100) and anti-phospho-synuclein (pSer129 rabbit ...
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bioRxiv - Cell Biology 2023Quote: ... The tissues were incubated at 4°C overnight with the following primary antibodies diluted in the antibody diluent containing 0.1% Triton X-100 and 2% Normal donkey serum: Mouse anti-ZO-1(Fisher Scientific, catalog #339100) (1:100) ...
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bioRxiv - Immunology 2023Quote: ... This medium consisted of basal crypt media (Advanced DMEM/F12, penicillin/streptomycin, 10 mM HEPES, 2 mM Glutamine) supplemented with 1× B27 (Gibco, 17504-044), 1× N2 (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... 0.2% BSA) the nuclei were incubated for 30 min at room temperature with Donkey x Goat-Alexa647 (1:400, ThermoFisher, # A-21447). Nuclei were washed twice with WB Wash buffer and resuspended in Sorting buffer (w/o RNase inhibitors) ...
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bioRxiv - Neuroscience 2023Quote: ... DRG samples were incubated in the blocking solution with added primary antibodies: anti-GFP (Invitrogen, #11122, 1:400, 2 days, 37°C), and anti-V5 (Invitrogen ...
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bioRxiv - Pathology 2023Quote: ... appropriate secondary antibodies were treated for 1-2 hours in the dark (AF555 anti-Rabbit, AF555 anti-Rat, AF647 anti-Rat (Invitrogen, Waltham, MA)) and washed in PBS containing 0.01% Triton X-100 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed in PBS and incubated for 2 h at room temperature with a goat anti-chicken Alexa 488 secondary antibody (#A11039, 1/500, Invitrogen, MA, US) and goat anti-mouse Alexa 555 (#A21422 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed in PBST and subsequently incubated for 2 hrs at room temperature in AlexaFluor™ 488-conjugated goat anti-rabbit (1:500, Invitrogen A11008) diluted in PBST containing 4% NGS ...
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bioRxiv - Immunology 2023Quote: Ex vivo isolated and rested CD8+ T cells or transduced T cells from splenocytes were incubated with R10 (RPMI 1640 + 10% FBS + 1% penicillin/streptomycin + L-glutamine + 20 U/ml of IL-2; Gibco, Thermo Fisher Scientific) alone or R10 containing PMA/ionomycin for two hours ...
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Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesisbioRxiv - Molecular Biology 2023Quote: ... Stable T-REx lines were generated by transfecting 1 μg of pcDNA5 FRT/TO 3xFLAG MiniTurboID and 2 μg of pOG44 (Invitrogen, Cat# V600520) with Lipofectamine3000 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µl polar metabolite extract were injected in push partial mode using an overfill factor of 1 onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 μm particle size, Thermo Fisher Scientific), additionally this was equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Cell Biology 2023Quote: ... followed 1 µg/ml donkey anti-mouse secondary and 2 µg/ml streptavidin-Alexafluor-488 reagent (to visualise the biotin, Thermo Fisher Scientific). Nuclei were counterstained with 10 µg/ml DAPI (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... for 2 hours at room temperature before overnight incubation with rabbit polyclonal anti-ELAPOR1 antibody (Thermo Fisher Scientific, PA5-72691, 1:150). Cells were then washed three times with PBS for 15 min and incubated with anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were washed with PBS to remove unbound antibody and incubated for 2 h with Alexa Fluor-conjugated secondary antibodies (Invitrogen; 1:500), 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Developmental Biology 2023Quote: ... three T175 flasks of Ishikawa cells at 60-70% confluence were supplemented with 1 µM 5-ethynyl-2’-deoxyuridine (EdU, Thermo Fisher Scientific) and incubated overnight to allow EdU incorporation into the DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... brain slices were washed 3X in 1X TBS and then incubated for 2 hours at RT with a fluorescent-tagged Alexa Fluor 568 anti-mouse secondary antibody (1:500; Life Technologies A11004). Brain sections were then washed 3X in 1X TBS ...
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bioRxiv - Microbiology 2023Quote: The human monocyte cell line THP-1 (MerckKGaA) were maintained at 2 × 105 cells/mL in RPMI-1640 containing 10% fetal bovine serum (FBS) (Thermo Fisher Scientific), 200 U/mL penicillin/streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... and E (SAgs, 2 μg/ml of each) for 1 h in serum-free AIM V™ medium (#12055-091; Gibco™). Unpulsed CFSE-stained Raji B cells were used as negative control (- SAgs) ...
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bioRxiv - Immunology 2024Quote: ... burgdorferi were resuspended in FACS buffer (30 μL/well) (200 μL; 2% fetal bovine serum in PBS supplemented with EDTA (1 mM, Thermo Fisher Scientific)) containing CV1-G4 or MIAP410 (10 μg/mL) ...
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bioRxiv - Immunology 2024Quote: ... the microtiter plates were washed 5 × with T-PBS and then incubated for 1 hour at room temperature with 2 μg/ml of biotinylated recombinant protein G (PierceTM, Thermo Fisher Scientific) for recognition of IgG antibodies from human ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The immunization emulsion consisted of syngeneic CNS antigen (1 part brain to 2 parts of spinal cord) in phosphate-buffered saline (Thermo Fisher Scientific) emulsified 1:1 in complete Freund’s adjuvant (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed 3 x 5 minutes in 1x PBS and incubated for 2 hours with the corresponding secondary antibodies goat anti-rabbit 488 (1:500; Fisher Scientific A11008) and goat anti-chicken 633 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... before being washed with PBS-T and incubated for 2 hrs at room temperature with 1:5,000 goat anti-mouse horseradish peroxidase-conjugated secondary antibody (Thermo Fisher Scientific; #G21040) diluted in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then washed with PBS-T and incubated for 2 hrs at room temperature with 1:3,000 goat anti-rabbit horseradish peroxidase-conjugated secondary antibody (Thermo Fisher Scientific; #G21234) diluted in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... cochleae were plated in six-well plates (2-3 explants/well) containing 1 ml media (growth medium DMEM (12430-054,Gibco Life Technologies) combined with 1% FBS (16000-044 ...
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bioRxiv - Immunology 2024Quote: ... with T-PBS and then incubated for 1 hour at room temperature with 2 µg/mL biotinylated Protein-G (Thermo Fisher Scientific) in T-PBS ...
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bioRxiv - Physiology 2024Quote: ... Samples were washed 3 times with 0.025% PBS-Tween20 and incubated with Alexa-conjugated secondary antibodies listed in Supplemental Table 2 (Life Technologies, 1/1000e) for 1 h ...
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bioRxiv - Cell Biology 2024Quote: The desired number of wells in a 96-well plate (MGB096-1-2-LG-L; Matrical, Inc.) or an 8-well Lab-Tek II chamber (155409; Thermo Fisher Scientific) were coated with 100 µL or 200 µL of 40 µg/µL porcine fibronectin (prepared from whole blood ...
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bioRxiv - Immunology 2024Quote: ... viremia samples or organ samples in 96-well plates in MEM (Sigma, M0446; supplemented with 2 % FCS and 1 % Penicilline/ Streptomycin (P/S; ThermoFisher, 15140-122)) ...
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bioRxiv - Cell Biology 2024Quote: ... devices were washed twice with PBS and then incubated for 2 h with Alexa Fluor Goat anti-mouse 488 (1:250, Invitrogen, A-11029) secondary antibody and Hoechst (5 μg/mL ...
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bioRxiv - Developmental Biology 2024Quote: ... in 2 mL complete human medium (DMEM/F12 [Gibco, Massachusetts, US], 10% FBS [PAA Cell Culture Company, UK], 1% GSP [Gibco, Massachusetts, US]). All cultures were maintained at 37 °C/5% CO2 in a humidified incubator ...
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bioRxiv - Immunology 2024Quote: ... Chem-1 cells stably-expressing C5aR1 (Eurofins) were labeled with the cell-permeable calcium binding dye Fluo-5F AM (Invitrogen, 2 μM) in the presence of probenecid (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... 2 ng/μL), CD8a (clone 53-6.7, 2 ng/μL), CD62L (clone MEL-14, 0.8ng/μL) from Thermofisher. For intracellular cytokine staining ...
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bioRxiv - Genomics 2020Quote: ... SARS-CoV-2 genome was amplified by using Ion AmpliSeq SARS-CoV-2 Research Panel (ThermoFisher Scientific, USA) that consists of two pools with amplicons ranging from 125 bp to 275 bp in length and covering >99% of the SARS-CoV-2 genome ...
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bioRxiv - Neuroscience 2019Quote: Isolated taste cells were incubated for 20 minutes in 2 μM Fura 2-AM (Invitrogen, Eugene, OR, USA) and nonionic dispersing agent ...
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bioRxiv - Immunology 2019Quote: Total splenocytes were incubated in PBS containing 2% fetal bovine serum and 2 mM EDTA (Gibco, ThermoFisher, USA). Fc receptors were first blocked using unconjugated anti-CD16/32 prior to staining with the following antibodies ...