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Citations for Thermo Fisher :
7351 - 7400 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Membranes were washed 2 times with TBST and 1 time with TBS before being developed using SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific). Membranes were imaged using LAS ImageQuant System (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... and 1 μg of plasmid encoding ER-targeted ESCargo(FTV) per dish using 2 μL of Lipofectamine 2000 reagent (Thermo Fisher; 11668019). Neurons were incubated for 48 h post-transfection and then imaged using a Leica SP5 confocal microscope as described above ...
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bioRxiv - Cell Biology 2020Quote: ... and TagRFP-T-VAPB(1-218)-eMagB or TagRFP-T-eMagB-PHOSBP (prey) and iRFP-P4C at a 3:2:1 ratio in OptiMEM-I (Thermo Fisher Scientific) (1:4 DNA ...
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bioRxiv - Cell Biology 2020Quote: ... labelled with the fluorescent lipid dye BODIPY® FL Maleimide [BODIPY® FL N-(2-Aminoethyl) Maleimide] (Thermo Fisher Scientific; 1:200) and incubated at room temperature in the dark 10 minutes prior to analysis ...
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bioRxiv - Immunology 2021Quote: ... hybridoma cells were adapted to and expanded in serum free medium containing 1:2 vol/vol Cell™ MAB medium (Life Technologies) and HyClone™ CDM4MAB medium (GE Life Sciences) ...
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bioRxiv - Immunology 2021Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The two Silencer Select negative control siRNAs (Silencer Select Negative Control No. 1 siRNA and Silencer Select Negative Control No. 2 siRNA) were purchased from Life Technologies (Ambion). In experiments where GBP1-5 were individually knocked down ...
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bioRxiv - Developmental Biology 2021Quote: ... Islets were handpicked twice and then incubated in regular islet media (RPMI 1640, supplemented with 5 mM glucose, 10% fetal bovine serum, 2 mM glutamine, 1% antibiotics (Gibco, Gaithersburg, MD)) at 37°C for 3 hrs or ...
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bioRxiv - Developmental Biology 2020Quote: ... the ovaries were incubated for 2 h at room temperature with secondary Alexa 568-conjugated antirabbit IgG (1:500 in PBX; Invitrogen, Life Technologies), washed and counterstained with Hoescht (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and in the secondary antibody diluted in 10% normal donkey serum for 2 hours at room temperature [Secondary antibody: Alexa Fluor 647 goat anti-rabbit (1:500; Thermo Fisher Scientific)].
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bioRxiv - Developmental Biology 2021Quote: ... while for the ECAR measurements the media was supplemented with 1 mM of Sodium Pyruvate and 2 mM of L-glutamine (both from Life Technologies, UK), pH 7.4 at 37°C.
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bioRxiv - Neuroscience 2021Quote: ... 3-3.5 µg prestin plasmids and 0.4 µg eGFP plasmid were transfected to HEK 293 cells using 10 µl of Lipofectamine ® 3000 (reagent 2:1 ratio; ThermoFisher Scientific) in 500-ml Opti-MEM (Life Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 3 times with 3X PBS for 10 min/each and 1 time with PBS and incubated for 2 h at room temperature with secondary antibodies - Alexa Fluor 555 donkey anti-sheep at 1:1000 dilution (Invitrogen, cat# A21436), Alexa Fluor 647 donkey anti-mouse at 1:200 dilution (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA products and DNA products were diluted to 1:10 and 2 μL was used as template for real time PCR reaction with PowerUp SYBR Green (Applied Biosystems, A25779). The primers used for both end point PCR and qRT-PCR analysis are listed in Supplementary Table S1.
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bioRxiv - Molecular Biology 2020Quote: ... which was replaced the next day with human neural induction media (DMEM HAM’s F12, with 50 μg/ml penicillin-streptomycin, 2 mM L-glutamine, 1:100 of N2 (Invitrogen, cat. 17502-048), 1:50 of B27 (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... or SARS-CoV-1-d28 (Genbank AAP13567) or SARS-CoV-2 VOC spike plasmid were incubated with transfection reagent Lipofectamine 2000 (Thermo Fisher, 11668027) following manufacturer’s instructions for 20 min at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... we used 5 μg plasmid DNA (with a concentration of at least 1 μg/μl) to 2 x 106 cells in R buffer for a 100 μl NEON electroporation pipette tip (Thermo Fisher Scientific) at 1600 V for 30 ms and 1 pulse ...
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bioRxiv - Neuroscience 2019Quote: ... primary antisera incubated sections were washed three times in PBS each for 10 minutes and subsequently incubated for 2 hours at room temperature in PBS with 2% NGS plus a dilution of an Alexa Fluor 488 goat anti-rabbit IgG (H+L) (1:1000, Molecular Probes, #A11034) or Alexa Fluor 594 goat anti-mouse IgG (H+L ...
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bioRxiv - Cancer Biology 2019Quote: Viability was assessed after 8 weeks of culture by staining with 4 μM ethidium homodimer-1 and 2 μM calcein AM (Thermo Fisher Scientific). Any condition where at least one viable cell was identified was considered positive for the presence of viable cells ...
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bioRxiv - Epidemiology 2019Quote: ... for 2 h at room temperature and incubated overnight at 4°C with rabbit polyclonal anti-myeloperoxidase (MPO, 1:100, Invitrogen, CA, USA) or rabbit monoclonal anti-F4/80 (1:100 ...
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bioRxiv - Immunology 2020Quote: ... was then used to electroporate 1-2 million NT or CAR-transduced NK cells using the Neon transfection system (Thermo Fisher Scientific). Alt-R HiFi Cas9 Nuclease 3-NLS (IDT ...
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Cell culture dimensionality influences mesenchymal stem cell fate through cadherin-2 and cadherin-11bioRxiv - Cell Biology 2019Quote: ... Primary antibodies used were rabbit polyclonal anti-cadherin-11 and mouse monoclonal anti-cadherin-2 (3B9) (both 1:100; Thermo Fisher Scientific). Secondary antibodies used were goat anti-mouse Alexa Fluor 488 and goat anti-rabbit Alexa Fluor 647 (both 1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... RNA interference was done by transfecting 100 nM siRNA (TPD54 1: GUCCUACCUGUUACGCAAU, TPD54 2:CUCACGUUUGUAGAUGAAA, TPD54 3:CAUGUUAGCCCAUCAGAAU) with Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... qRT-PCR was performed with a 1:2 dilution of cDNA by using the StepOnePlus Real-Time PCR-System (Thermo Fisher Scientific) or the CFX384 Touch™ Real-Time PCR detection system (Bio-Rad ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated 2 hours at room temperature with the corresponding secondary antibodies—Alexa fluor 633 conjugated goat anti-rabbit (1:500; ThermoFisher A-21070) and Alexa fluor 633 conjugated goat anti-mouse (1:500 ...
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bioRxiv - Immunology 2021Quote: ... Immunoprecipitated samples were subjected to Proteinase K digestion in wash buffer supplemented with 1% SDS and 2 mg/mL Proteinase K (Thermo Fisher Scientific) incubated with shaking at 1200 rpm at 55□ for 1 h ...
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bioRxiv - Biophysics 2021Quote: ... was introduced into flow cells at 1 ~ 2 μl/min in the T50 buffer with 200 nM Sytox orange (ThermoFisher Scientific; S11368). Excess DNA was washed off with T50 buffer once optimum DNA density was achieved.
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bioRxiv - Immunology 2021Quote: ... were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The frozen tissue samples were homogenized in 1 ml DMEM-2 manually in a disposable 15 ml closed Tissue Grinder System (Thermo Fisher Scientific). 140 µl out of 1ml samples were used for RNA extraction while 500 µl of homogenates were used for quantification of SARS-CoV-2 ...
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bioRxiv - Cancer Biology 2020Quote: ... PBMC were maintained in complete RPMI 1640 and treated directly with MEDS433 alone or with Dypiridamole (0.1–1 μM) or stimulated with 2% phytohemagglutinin (PHA, Gibco, Thermo Fisher Scientific), IL2 200U/ml (Peprotech ...
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bioRxiv - Bioengineering 2021Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY™ FL C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine (PED-A1) and BODIPY™ FL C5 were purchased from Thermofisher scientific ...
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bioRxiv - Biochemistry 2020Quote: ... after which media was removed and the cell monolayer washed 1 time with 2 ml cold DPBS with calcium and magnesium (DPBS++, Invitrogen, #14040-133). The DPBS++ was removed and 100 ul of ice cold lysis buffer was added containing ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated at RT for 2 hours with secondary antibodies diluted at 1/500 (coupled with Alexa 488 or 568 - Thermo Fisher Scientific) and with Methoxy X04 (5 μM ...
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bioRxiv - Neuroscience 2021Quote: ... hMOs were then washed overnight with 1X PBS at 4°C and incubated with Hoechst 33342 solution in 1X PBS for 2 h (1:1000, Thermo Fisher Scientific) and washed in PBS for 2 h ...
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bioRxiv - Neuroscience 2020Quote: ... with 1:1000 dilution in staining buffer for 2 hours at room temperature and stained with DAPI (300 nM, D1306, Thermo Fisher Scientific) for 10 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... washed 3 times with blocking buffer and incubated for 2 h with Alexa 647 anti-rabbit (1/250, A21247, Thermo Fisher Scientific). After washing the cells 3 times with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... approximately 1 pmol injected onto a Acclaim PepMap 100 guard column (75 μm×2 cm C18, 3 μm particles, 100 Å; Thermo Scientific) and separated on a 50 cm PepMap RSLC C18 (75 μm×50 cm ...
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bioRxiv - Microbiology 2020Quote: Two milli-litres of O+ erythrocytes at 2% hematocrit in incomplete-culture media were labelled using 2 µl of a stock of 1 mM Far-red Cell Trace (Thermo Fisher Scientific); control unstained erythrocytes were incubated with 2 µl of Dimethyl-sulphoxide (DMSO ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 hours at RT with appropriate secondary antibodies: goat anti-rabbit Alexa Fluor633 (1:200, ThermoFisher, A-21070), and donkey anti-mouse Alexa Fluor488 (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were subsequently incubated in the dark for 2 hours at room temperature with secondary antibody donkey anti-rabbit IgG conjugated to AlexaFluor-594 (1:250; Invitrogen, A-21207). After washing with PBS ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were transiently co-transfected into Expi293F cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ 293 Reagent (Thermo Fisher Scientific) according to the manufacturer” s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... a 1-mL aliquot of BY-2 cell culture was mixed with CellTracker Green CMFDA Dye (C7025, Thermo Fisher Scientific, Tokyo, Japan) at 5 μM and incubated for 30 min at 26°C with agitation ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 h for cells or 2 h for tissues at room temperature and the nuclei were stained with Draq5 (1:1000, Thermo Fisher Scientific) for 40 min or 4′,6- diamidino-2-phenylindole (DAPI ...
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bioRxiv - Microbiology 2021Quote: ... Reporter-expressing rSARS-CoV-2 were detected directly by epifluorescence and using either Alexa Fluor 594 goat anti-mouse IgG (Invitrogen; 1:1,000) or fluorescein isothiocynate (FITC)- conjugated goat anti-mouse IgG (Dako ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2 h incubation with secondary antibody goat-anti-rabbit Alexa Fluor 488 (1:500, #A11008, Invitrogen-Thermo Fisher, MA, USA). Sections were transferred to glass slides and covered with Fluoromount (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2 h incubation with secondary antibody goat-anti-rabbit Alexa Fluor 488 (1:500, #A11008, Invitrogen-Thermo Fisher, MA, USA). Sections were transferred to glass slides and covered with Fluoromount (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... and THP-1 cells (100 μl of 2 x 105 cells/mL) were seeded in 96 well tissue culture plate (Nunc™, Thermofisher) for 24 hours using DMEM and RPMI media respectively supplemented with 5% FBS in 5% CO2 incubator at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Two hundred microliters of 2 μM calcein AM/4 μM ethidium homodimer-1 in PBS (Live/Dead® Viability Kit, Invitrogen, USA) were added to the wells and plates incubated for 30 min in the dark ...