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Citations for Thermo Fisher :
7401 - 7450 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... followed by incubation with PBST + 2% normal goat serum and goat anti-rabbit DyLight 488 conjugated secondary antibody (1:2000) (Fisher Scientific #35552). Larvae were imaged at 10X using a Nikon Ti2-E epifluorescent microscope.
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bioRxiv - Neuroscience 2020Quote: ... and for 2 hr with secondary goat anti-mouse Alexa Fluor Plus 488 antibody (Cat no. A32723; Thermo Fisher Scientific; 1:1000). Cells were then incubated overnight at 4 °C with PE-conjugated LAMP1 CD107a clone H4A3 antibody (Cat no ...
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bioRxiv - Microbiology 2022Quote: ... recovered sporozoites cells were stained with anti-PvCSP mAbs 1:500 and fixed 10 min in 100uL of a 2% PFA solution (Thermo Scientific™) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... The primary antibody incubation was followed by 2 h incubation at room temperature with corresponding fluorescent secondary antibodies (1:500, Thermo Fisher Scientific). Nuclei were counterstained with DAPI (1:2000 in 0.1 M PB ...
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bioRxiv - Cell Biology 2022Quote: ... coverslips were incubated for 2 hours in a 1:1000 dilution of secondary antibody (anti-rabbit, Invitrogen, 32260; anti- mouse, Invitrogen, 32230). Coverslips were washed 3 times with blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... They were stained for 2 days at 4°C with a mouse-derived antibody against GFP (1:400, mAB 3E6, Invitrogen, Darmstadt, Germany), washed for 2 hours at RT in 1x PBS ...
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bioRxiv - Biochemistry 2022Quote: ... The MTS were stained with a mixture of 2 μM calcein AM and 4 μM ethidium homodimer-1 in PBS (Thermo Fisher Scientific) to stain for live and dead cells ...
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bioRxiv - Microbiology 2022Quote: ... pellets were resuspended in 1 mL of Dynabuffer Isolation Buffer 2 for magnetic bead separation with Dynabeads® CD45 and Dynabeads® CD31 (ThermoFisher) as from manufacturers instructions to remove any possible contamination of macrophages and endothelial cells respectively ...
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bioRxiv - Molecular Biology 2022Quote: ... 25 µM customized Nextera PCR primers 1 and 2 and 0.6% final concentration of 100X SYBR Green I (Invitrogen Cat #S-7563) in a thermocycler with the following parameters ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed to remove the excess of primary antibody and incubated with the appropriate Alexa-Fluor secondary antibodies for 2 h at 25 °C (1:1000, Thermo Fisher Scientific). The excess of secondary antibody was washed ...
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bioRxiv - Immunology 2022Quote: Bma-LEC-1 and -2 PCR products were digested and ligated into pOET1C 6xHis Transfer plasmid using T4 DNA ligase (Thermo Fisher Scientific), transformed into ampicillin resistant NEB alpha competent E ...
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bioRxiv - Immunology 2022Quote: ... with PBS-T followed by a 2 h incubation on a shaker at room temperature with goat anti-rabbit IgG-HRP (1:5000) (Thermo Fisher Scientific) in blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody solution contained 2 secondary antibodies in blocking solution: Goat anti-mouse Alexa-Flour (AF) 568 (Invitrogen A-11004, 1:400), Goat anti-chicken AF-488 (Invitrogen A11039 ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were washed three times in 0.5% PBS-T for 10 min and incubated for 2 hours at room temperature with secondary conjugated antibodies (1:500) from Invitrogen (Molecular Probes), including Alexa488 goat anti-rabbit IgG ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2 h incubation with secondary antibody goat-anti-rabbit Alexa Fluor 488 (1:500, #A11008, Invitrogen-Thermo Fisher, MA, USA). Sections were transferred to glass slides and covered with Fluoromount (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2 h incubation with secondary antibody goat-anti-rabbit Alexa Fluor 488 (1:500, #A11008, Invitrogen-Thermo Fisher, MA, USA). Sections were transferred to glass slides and covered with Fluoromount (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... cells or tissue explants were incubated with virus (MOI 0.05-5) for 1-2 hr or overnight respectively at 37°C using fresh media supplemented with fetal bovine serum (Thermo Fisher Scientific). For co-infection assays ...
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bioRxiv - Microbiology 2021Quote: ... IgG or Fab were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed in PB (3 × 10 min) and incubated for 2 h at room temperature with a secondary Alexa Fluor 488 donkey anti-goat antibody (Invitrogen, 1:500). Sections were rinsed in PB again (3 × 10 min ...
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bioRxiv - Microbiology 2020Quote: ... plates were washed six times with PBS (200 μl) and then blocked for 1-2 hours with Goat Serum (ThermoFisher Scientific, 16210072) at 37°C ...
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bioRxiv - Microbiology 2020Quote: TSA assays were performed by mixing 5 μL protein solution at 2 mg/mL with 5 μL SYPRO®Orange (1:50 diluted from 5000x stock solution, Invitrogen, USA), 5 μl of 8-fold buffer (1200 mM NaCl and 400 mM Tris pH 8) ...
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bioRxiv - Neuroscience 2020Quote: ... 15 min each and then incubated the brain sections in 2° antibodies (1:700 for Dylight-/Alexa-conjugated antibodies made in donkey purchased from Thermo Fisher Scientific) in 5% BSA/PBST for 2 hrs at RT ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 h with the appropriate secondary antibody (AlexaFluor™ 488 goat anti-rabbit IgG; 1:1,000; Molecular Probes, USA). Sections were then washed in TBS 0.05M ...
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bioRxiv - Immunology 2020Quote: ... The morphological characteristics of undifferentiated and differentiated THP-1 cells were captured by EVOS FL Auto Cell Imaging System 2 (Thermo Fisher Scientific) using a 40x objective lens and were processed by ImageJ software (W.S ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 2 hrs in secondary antibody Alexa Fluor 594 goat anti-rabbit IgG (1:500 in dilution buffer, catalog # A11012, Invitrogen, Carlsbad, CA). Sections were rinsed thrice in TBS and twice in double distilled water (5 mins each) ...
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: ... MP029-CRY2olig-mCh-SUN2-N-1 or MP029-CRY2olig-mCh-SUN2-N-2 using Lipofectamine LTX with Plus Reagent (Thermo Fisher Scientific) according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies, 25030-024], 1 mM sodium pyruvate [Gibco Life Technologies ...
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bioRxiv - Immunology 2022Quote: ... were transfected with plasmids carrying J08 and 02M04 antibody heavy and the light chains with a 1:2 ratio using ExpiFectamineTM293 Transfection Kit (Thermo Fisher Scientific) as recommended by the manufacturer ...
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bioRxiv - Immunology 2022Quote: ... were used at a bead: cell ratio of 1:2 and the activated cells were cultured in complete RPMI 1640 medium (Thermo Fisher Scientific) or complete 1640 RMPI no glucose medium supplemented with IL-2 (10 ng/ml) ...
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bioRxiv - Immunology 2022Quote: FLAG-DNAM-1-expressing human Treg cells were co-cultured in complete RPMI-1640 culture medium with 2 × 104 CellTrace Violet (Thermo Fisher Scientific)-labeled Tconv cells (freshly isolated from the same donor as that of the Treg cells ...
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bioRxiv - Microbiology 2022Quote: ... The viral genome between 27,895-29,534 nucleotides based on the SARS-CoV-2 WA-1 strain was RT-PCR amplified using Super Script II Reverse transcriptase (Thermo Fisher Scientific) and Expanded High Fidelity PCR system (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... cells were washed with PBS and incubated for 2 hours at room temperature in 1:500 Alexa Fluor 568 (Thermo Fisher #A11011). After final washes in PBS ...
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bioRxiv - Microbiology 2022Quote: ... bacterial were incubated in hot phenol lysis solution (1% SDS, 2 mM EDTA, 40 mM sodium acetate in acid phenol (Invitrogen #15594-047)) for 45 minutes at 65 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 45 s in air at 15 mA and 0.4 mbar) holey carbon grid (C-flat: 2/1-3C-T) and vitrified using a FEI Vitrobot Mark IV (Thermo Fisher Scientific) after blotting for 3 seconds with blot force 18 ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were lysed in a hot (60 °C) phenol solution (2 mM EDTA, pH8, 1% SDS, 40 mM sodium acetate in acid phenol (Invitrogen #15594-047)) ...
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bioRxiv - Developmental Biology 2019Quote: ... while for the ECAR measurements the media was supplemented with 1 mM of Sodium Pyruvate and 2 mM of L-glutamine (both from Life Technologies, UK), pH 7.4 at 37ºC.
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Lateral organ diversification in plants mediated by the ALOG protein family of transcription factorsbioRxiv - Plant Biology 2019Quote: Gemmalings were incubated in 1/2 B5 medium containing 10uM EdU (Click-iT Edu Alexa Fluor 488 imaging kit; Thermo Fisher, USA) for 3h ...
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bioRxiv - Biochemistry 2019Quote: ... Resulting peptide mixtures (1 µL) were loaded onto a PepMap 100 C18 trap column (75 µm id x 2 cm length; Thermo Fisher Scientific) at 3 µL/min for 10 min with 2 % acetonitrile and 0.05 % trifluoroacetic acid followed by separation on an Acclaim PepMap RSLC 2 µm C18 column (75µm id x 25 cm length ...
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bioRxiv - Bioengineering 2020Quote: The rADSCs of the 4th passage were seeded on a six-well culture plate at a density of 5 ×105 cell/ml with 1 ml of neurosphere medium containing serum-free DMEM/F12 with 2 % B27 supplement (Thermo Fisher, USA) and 20 ng/ml of basic fibroblast growth factor (bFGF ...
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bioRxiv - Neuroscience 2019Quote: ... 3 PBS washes were performed and then 2 hours of incubation in CY-5 goat anti-rabbit (1:1000, Invitrogen, ThermoFisher Scientific, A10523) diluted in 2%NGS-PBS ...
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bioRxiv - Systems Biology 2020Quote: ... Clone cultures were concentrated to 20-fold in M9 buffer and 10 μL was plated onto a well of a 96 well plate containing NGM agar with 2 mM Isopropyl β-d-1-thiogalactopyranoside (IPTG, Fisher Scientific). Plates were dried and stored at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were transfected with Silencer Select Pre-Designed siRNAs either as negative control (non-targeting, Silencer Select Negative Control No. 1 and 2 siRNA) or NFIB-targeting siRNA constructs (Thermofisher, s9494, s9496) using 30 pmol of siRNA in 9 μl of Lipofectamine RNAi Max ...
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bioRxiv - Genetics 2020Quote: ... Lipid extracts were vacuum evaporated and re-dissolved in 100 µl chloroform/methanol (1:2; v/v) and analyzed by MSALL [69] using an Orbitrap Fusion Tribrid (Thermo Fisher Scientific) equipped with a TriVersa NanoMate robotic nanoflow ion source (Advion Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... Cells infected with rSARS-CoV-2-Venus or -mCherry were washed with PBS and stained with either Alexa Fluor 594 goat anti-mouse IgG (Invitrogen; 1:1000) or fluorescein isothiocynate (FITC)-conjugated goat anti-mouse IgG (Dako ...
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bioRxiv - Immunology 2021Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... We matched MS/MS fragmentation spectra to human forward protein databases and against reverse databases to a 1–2% false discovery rate using the SEQUEST database search program (Thermo Fisher Scientific) [61] ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... incubating with primary antibodies (rabbit anti-β-actin, 1:1000, Thermo Fisher Scientific, PA1-183; mouse anti-claudin-2, 3:500, Invitrogen, 325600 ...
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bioRxiv - Microbiology 2021Quote: ... The plasmids were transiently co-transfected into Expi293F cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ 293 Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 2 μg of the DNA and RNA mixture was treated with 1 μl of DNase (2 μ/μl; Turbo DNase, TURBO DNase, ThermoFisher Scientific), followed by processing with an RNA Clean and Concentrator kit (Zymo Research) ...