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Citations for Thermo Fisher :
7501 - 7550 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The cells were washed with PBS three times and then incubated for 2 h with 1:1000 goat anti-mouse Alexa 488 (Catalogue no. A11029, Invitrogen, CA, USA) and 1:1000 goat anti- rabbit Alexa 59 (Catalogue no ...
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bioRxiv - Molecular Biology 2023Quote: ... and a 1:10 dilution of 2 μl of the samples was used to measure protein concentration by Qubit Protein Assay (Invitrogen, Cat# Q33211). The remaining 10 μl were reduced ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 h followed by incubation with rabbit anti-goat IgG conjugated with Q-dot®655 (1:350 dilution; Life Technologies) for 90 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... an equivalent volume of 2-propanol (∼200 to 300 µL) was added to the collected phase in addition of 1 µL of GlycoBlue™ (Invitrogen™) (a blue nucleic acid co-precipitant allowing a better visualization of the RNA pellet) ...
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bioRxiv - Neuroscience 2023Quote: Peptides were reconstituted in 100ul of 100mM triethyl ammonium bicarbonate (TEAB) and labeling performed as previously described (1, 2) using TMTPro isobaric tags (Thermofisher Scientific, A44520). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... All brain region slices were then incubated for 2 hours in a 1:1000 dilution of secondary antibody: goat anti-rabbit (Alexa Flour 594, Life Technologies; A11037). VTA slices also received goat anti-mouse (Alexa Flour 488 ...
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bioRxiv - Immunology 2022Quote: The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... at an effector/target ratio of 2:1 in the presence of mouse anti-human CD107a-FITC (11-1079-42; Thermo Fisher Scientific). Cocultures between CTLs and EndoC-βH1 cells expressing HLA-A2 were performed at 37 °C for 1.5 h in Iscove’s Modified Dlbecco’s Medium (IMDM ...
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bioRxiv - Plant Biology 2024Quote: ... PCR products were run on 1% or 2% TAE-agarose gels with 1000 bp or 50 bp GeneRuler (ThermoFisher Scientific Inc., Germany) depending on product size ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... PMA-treated THP-1 macrophages induced with LPS + IFNγ were labelled with Cell-tracker™ Orange CMRA (2 μM; Thermo Fisher Scientific). LPS-treated PBMCs were labelled with Cell Tracker™ Green CMFDA (20 μM ...
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bioRxiv - Plant Biology 2024Quote: ... protein concentrations of fractions containing FtsZ1-2 and FtsZ2-1 were determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2024Quote: ... Coverslips with attached cells were incubated for 30 min at 37 °C in ES containing 2 µM Calcium Green-1 AM (Invitrogen C3011MP, Thermo Fisher Scientific) and 0.02% Pluronic F-127 (Invitrogen P6867) ...
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bioRxiv - Cell Biology 2023Quote: ... First-strand cDNA was synthesized using a ratio of 2:1 random primers:oligo (dT) primer and SuperScript II RT (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... virus was serially diluted in Vero maintenance media (MEM supplemented with 2% FBS and 1% each of GlutaMax (Gibco, Grand Island, NY), PenStrep ...
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bioRxiv - Molecular Biology 2024Quote: ... First-strand cDNA was synthesized using a ratio of 2:1 random primers:oligo (dT) primer and SuperScript II RT (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... SARS-CoV-2 viral RNA was detected by reverse transcription and amplification using the TaqMan RNA-to-CT 1-Step Kit (Thermo Fisher Scientific), utilizing the primers and probe sequences for viral RNAs are as described before [76] ...
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bioRxiv - Neuroscience 2024Quote: ... 0.2% Triton-X 100 and 2 % BSA in PBS: Alexa 488-conjugated donkey anti-rabbit (1:500, Life technologies/ Invitrogen, Cat# A11055), Alexa 555-conjugated donkey anti-mouse (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... 0.2% Triton-X 100 and 2 % BSA in PBS: Alexa 488-conjugated donkey anti-rabbit (1:500, Life technologies/ Invitrogen, Cat# A11055), Alexa 555-conjugated donkey anti-mouse (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... in blocking buffer followed by PBS wash 15 min three times and then incubation for 2 h at room temperature with Alexa Fluor 594 conjugated donkey anti-rabbit antibody (1:1000, ThermoFisher A-21207). Stained samples were then washed for 15 min three times in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... at 1:5000 for 2 hours and developed using Pierce ECL Western Blotting Substrate or SuperSignal West Femto Substrate (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2023Quote: ... at a flow rate of 75 μL/min on a 15-cm C18 column maintained at 40°C (Acclaim Pepmap 1 mm I.D., 2 μm particle size, 100 Å pore size; Thermo Fisher Scientific). The LC method for DDA starts at 2% B and is held constant for 1 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Codon-optimized gene encoding the full-length SARS-CoV-2 PLpro (1-315 amino acids) was custom synthesized (Invitrogen, Thermo Fisher Scientific) and was cloned using XhoI and NdeI restriction sites in pET28c vector containing an N-terminal 6X histidine tag ...
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bioRxiv - Molecular Biology 2023Quote: ... Codon-optimized gene encoding the full-length SARS-CoV-2 PLpro (1-315 amino acids) was custom synthesized (Invitrogen, Thermo Fisher Scientific) and was cloned using XhoI and NdeI restriction sites in pET28c vector containing an N-terminal 6X histidine tag ...
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bioRxiv - Cell Biology 2024Quote: ... The pellets were resuspended in cold lysis buffer (Table EV5) supplemented with protease and phosphatase inhibitors (2 mM Na3VO4, 1 tab/10 ml; Thermo Fisher Scientific). Cell suspensions were lysed using mechanical shearing by pipetting and then sonicated (10-15 pulses at 50% power) ...
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bioRxiv - Cell Biology 2023Quote: ... cells at 70-80% confluency were switched to myogenic differentiation medium (DMEM, 4% horse serum, 2 mM Glutamax, 1 mM sodium pyruvate (Life Technologies #11360070), 1 μg/mL insulin (GeminiBio #800122 ...
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bioRxiv - Cell Biology 2023Quote: ... The lysates were digested overnight at 37°C with 1-2 µL of the Lys-C (125-05061, Wako) and trypsin mixtures (Pierce, Thermo Fisher Scientific) (10 ng/μL each) ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA pol I and II visualization was accomplished by blocking (2) and incubating samples with rabbit anti-p-UBF1 (1:100 dilution; Invitrogen, PA5-105512) and mouse anti-RNA pol II (1:100 dilution ...
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bioRxiv - Immunology 2024Quote: ... 1:400 and for 1 hour at room temperature with the quaternary antibody: F(ab)’2-goat anti-rabbit IgG (H+L) A488 (Invitrogen, A-11070) 1:1000 ...
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bioRxiv - Physiology 2023Quote: ... IALVs were then washed with PBS containing 0.1% Triton X-100 (PBST) 3 times and blocked for a minimum of 2 hr with Blockaid® (B-10710, ThermoFisher Scientific). IALVs were then stained with the corresponding primary antibodies in BlockAid® Solution ...
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bioRxiv - Bioengineering 2024Quote: MSC conditioned media was prepared by culturing primary MSCs (2×105 cells) or hTERT-MSCs (1×106 cells) in R10 media for 48-72 hours (RPMI Medium 1640 (Gibco, Waltham, MA) with 10% heat-inactivated Fetal Bovine Serum ...
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bioRxiv - Cell Biology 2023Quote: between 0.2·106-1·106 of VS derived cells were reprogrammed using the CytoTune™-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific), a non-integrative cell reprogramming method holding the four Yamanaka factors (OCT4 ...
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bioRxiv - Biochemistry 2024Quote: ... The amine group of the purified DNA constructs was further modified to maleimide by incubating the sample with 1 mM SM(PEG)2 (Thermo Scientific Pierce) for 20 min at ∼23°C ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed 4-5 times in PBS for 2 hours then incubated for 3-4 hours with secondary antibody (anti-rabbit Alexa Fluor 568; 1:1000; Invitrogen; Cat #A10042) and NeuroTrace Green Fluorescent Nissl Stain (1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... And then incubate the brain sections in 2° antibodies (1:700 for Dylight-/Alexa-conjugated antibodies made in donkey purchased from Thermo Fisher Scientific) in 5% BSA/PBST and incubate for 2 hours at RT ...
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bioRxiv - Microbiology 2023Quote: The RBDs of G614, Alpha, Beta, and Omicron (BA.1, BA.2, BA.4/5) variants were ordered as GeneString from GeneArt (Thermo Fisher Scientific). All sequences of the RBD (aa 319-541 in GenBank ...
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bioRxiv - Immunology 2023Quote: ... Duplicate wells of 200,000 CFSE-stained cells per sample were then incubated for 91 hours at 37°C (5% CO2) at a 1:2 bead-cell ratio with Human T-Activator CD3/CD28 DynaBeadsTM (Thermo Fisher, #11161D) and 1 U/mL of recombinant human IL-2 (BioLegend ...
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bioRxiv - Molecular Biology 2023Quote: ... The transfection experiments were performed with pGL3 luciferase reporter plasmids separatly and in co-transfection with the pDiPRO1 vector using a 1:2 ratio of DNA (µg) to Lipofectamine™ 2000 transfection reagent (µl) (Invitrogen, Life Technologies). Myoblasts and RMS cells were seeded on 24-well culture vessels at 3.8×104 cells per well the day before transfection ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed two times with FACS buffer to remove any nonbounded Fc-hDectin1 and resuspended in FACS buffer containing a 1:200 dilution of the secondary antibody F(ab’)2-Goat anti-Human IgG conjugated to AlexaFluor™ 488 (ThermoFisher Scientific). This solution was incubated for 1 hour on ice before finally washing twice with FACS buffer and measuring the fluorescence intensity of 5 000 cells using a Guava® flow cytometer with 488nm excitation and fluorescence emission selected using a 522/30 bandpass filter.
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bioRxiv - Physiology 2023Quote: At day 45 (± 2 days) protein was extracted from beating iPSC-CMs in protein lysis buffer (1 X Pierce™ RIPA buffer (Thermo Fisher); 1 X PhosSTOP cocktail (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... E14 and E16 chick embryos in chilled L-15 mediumand subsequently incubated in 1 mM solution of 2-NBDG (N13195, Thermo Fisher Scientific) in L-15 medium at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... incubated at 65°C for 5 min and then immediately chilled on ice for at least 2 min before adding 4 μL reverse transcription reaction mix [in 10 μL RT reaction: 1× First Strand buffer (Invitrogen – 18064- 014), 20 mM DTT (Invitrogen - 18064-014) ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were incubated for 2 h at room temperature with secondary antibody Alexa 488 goat anti-rabbit IgG or rhodamine-phalloidin (1:500, Invitrogen, Molecular Probes). Samples were washed three times in 0.5% PBS-T and mounted in Vectashield containing DAPI.
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bioRxiv - Genomics 2023Quote: ... for 1 hour, followed by incubation with primary antibodies (overnight, 4°C) and secondary antibodies (1-2 hours, from Jackson ImmunoResearch or ThermoFisher Scientific). The slides were then mounted with coverslips using VECTASHIELD (Vector Labs ...
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bioRxiv - Immunology 2023Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (heavy chain to light chain) using ExpiFectamine CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... CTLA-4 or PD-1) were suspended in IL-2 medium at 37°C containing either 65nM LysoTracker® Deep Red (ThermoFisher Scientific) or 25nM MitoTracker™ Red CMXRos for 1h or 30min ...
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bioRxiv - Microbiology 2023Quote: ... and two Silencer Select negative-control siRNAs (Silencer Select negative control no. 1 and no. 2 siRNA) were purchased from Ambion (Life Technologies). Three days before infection ...
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bioRxiv - Bioengineering 2022Quote: ... Incubation with secondary antibodies diluted in blocking solution was performed for 2 h at RT: Anti-mouse-Alexa 647 (1:500, cat. A32787, ThermoFisher Scientific Inc.), Alexa Fluor 488-Phalloidin (1:500 ...
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bioRxiv - Bioengineering 2022Quote: ... retinas were incubated for 2 hours at RT with 546-conjugated anti-rabbit IgG antibody (1/300; Life Technologies, Saint Aubin, France). Cell nuclei were labelled with Draq5 (1/500 ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5 mL of a 1–2 mg/mL protein sample was mixed with 2.5 µL of SYPRO® orange dye (ThermoFisher Scientific, USA) and aliquoted at 5 µL before being mixed with 45 µL of the respective screening buffer ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane was incubated overnight with SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) rabbit polyclonal antibody (Thermo Fisher scientific, France) at a dilution of 1:1000 in the same buffer as for saturation ...