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Citations for Thermo Fisher :
701 - 750 of 1284 citations for Fmoc Lys Boc Rink Amide Resin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Proteins were then run through a 4mL bed of HisPur Ni-NTA resin (Thermo Scientific) with a wash step of I0 buffer (where the desired cleaved protein will come out ...
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bioRxiv - Molecular Biology 2023Quote: Nuclear extracts were pre-cleared with an inert resin Protein A Agarose (Thermo Fisher Pierce) for 2h at 4°C with rotation ...
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CXCL17 binds efficaciously to glycosaminoglycans with the potential to modulate chemokine signallingbioRxiv - Immunology 2023Quote: ... pcDNA3.1-CXCL17 and mock SN was concentrated with 50μL HisPur™ Ni-NTA Resin (ThermoFisher). Transfected cells were analysed by flow cytometry to confirm transfection efficacy and protein expression after 48 hours ...
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bioRxiv - Microbiology 2023Quote: ... and the supernatant was run over 2 ml of Ni-NTa resin (Thermo Fisher Scientific) equilibrated with Buffer 1 ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was harvested and the S trimer was purified using Ni-NTA resin (Invitrogen) in accordance with the manufacturer’s protocol five days after transfection ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies and their isotype control IgG were immobilized to AminoLink Coupling Resin (ThermoFisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... mixed with 50 µL of high-capacity Neutravidin agarose resin (Thermo Fisher Scientific, Schwerte, Germany), and incubated (inverting ...
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bioRxiv - Biophysics 2023Quote: ... Protease columns were prepared in-house by coupling the protease to a resin (Thermo Scientific POROS 20 Al aldehyde activated resin 1602906 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared extract was loaded onto 1 ml pre-equilibrated Ni-NTA resin (Thermo Scientific). The column was washed extensively with nickel buffer then eluted in buffer containing 500 mM imidazole ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared extract was loaded onto 1 ml pre-equilibrated Ni-NTA resin (Thermo Scientific). The column was washed extensively with nickel buffer (25 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The supernatant containing the periplasm was applied to 2 ml HisPur Cobalt resin (Thermo Fisher) and incubated for 2 hours at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Equal amounts of tissue lysates were pre-cleared using the Control Agarose Resin (Thermo Fisher). The antibody-antigen complexes were incubated overnight at 4°C and eluted following Pierce Co-Immunoprecipitation (Co-IP ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was flowed over 6 ml of washed Ni-NTA resin (ThermoFisher Scientific) in a gravity column ...
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bioRxiv - Immunology 2024Quote: ... all protein constructs underwent endotoxin removal using Pierce High-capacity endotoxin removal resin (ThermoFisher Scientific). Subsequently ...
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bioRxiv - Bioengineering 2024Quote: ... The supernatant fraction was incubated with Ni-NTA beads (HisPurTM Ni-NTA Resin, Thermo Scientific) pre-equilibrated in buffer containing 8M urea ...
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bioRxiv - Immunology 2024Quote: ... Primary antibodies and their isotype control IgG were immobilized to AminoLink Coupling Resin (ThermoFisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and pH 8.0 before binding to the Ni-NTA resin (Invitrogen™, Waltham, MA, USA). The resin was washed ...
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bioRxiv - Microbiology 2024Quote: ... The cleared lysate was incubated with 1 mL HisPur Ni-NTA agarose resin (Thermo Fisher) for 1 h at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... while GST-fusion proteins were enriched PierceTM glutathione agarose resin (Thermo Fisher Scientific, Waltham USA). Both resins were packed into PierceTM centrifugation columns (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... supernatant was subjected to HisPur™ Ni-NTA Resin (88222; Thermo Fisher Scientific, Weltham, MA) column and eluted in buffer B (buffer A supplemented with 50 mM imidazole) ...
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bioRxiv - Immunology 2024Quote: ... followed by removal of remaining His-tagged Fabs with HisPur Ni-NTA resin (Thermo Scientific). The thrombin-protease was removed via Streptavidin Sepharose High Performance affinity resin (Cytiva) ...
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bioRxiv - Biophysics 2024Quote: ... The solution was then loaded onto 5 ml pre-equilibrated Ni-NTA resin (ThermoFisher Scientific) on a gravity flow column and washed with 10 column volumes of a buffer containing 50 mM NaPi ...
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bioRxiv - Biochemistry 2024Quote: ... IgGs were incubated with 0.250 ml of the 50% slurry of Immobilized Papain resin (ThermoFisher) followed by incubation with digestion buffer containing 35 mg cysteine at 37 °C overnight in microcentrifuge tubes ...
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bioRxiv - Biochemistry 2024Quote: ... The clarified supernatant was transferred to a HisPur Ni-NTA resin column (Thermo Fisher Scientific) pre-equilibrated with lysis buffer.
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bioRxiv - Biophysics 2024Quote: ... The NiNTA resin was then packed into a gravity flow column (Thermo Scientific disposable polypropylene) and washed with 120 mL of buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was filtered and applied to a TALON HisPur cobalt resin (Thermo Scientific #89964). Proteins were eluted in a buffer containing 20 mM phosphate buffer pH 8 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and the cleared lysates were incubated with 1 mL HisPur Ni-NTA Resin (Thermo Fisher Scientific), washed and eluted ...
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bioRxiv - Immunology 2022Quote: ... The secreted S-2P and S-6P proteins were purified using HisPurTM cobalt resins (Thermo Scientific) and StrepTactin resins (IBA) ...
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bioRxiv - Molecular Biology 2020Quote: ... The RSC1-TAP complex was cleaved from the IgG resin with 500 U AcTEV enzyme (Invitrogen) gently rotating the slurry at 15°C for 1 h followed by 4°C rotation overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... HUWE1 eluted from affinity resin was buffer-exchanged using Zeba Spin Desalting Columns (Thermo Fisher Scientific) and incubated with 1.5 mM bis(sulfosuccinimidyl)suberate (BS3 ...
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bioRxiv - Systems Biology 2020Quote: ... Abundant proteins were removed using the High Select Top14 Abundant Proteins Depletion Resin (Thermo Scientific A36372BR) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... The samples were pre-cleared with 100 μL of Pierce Control Agarose Resin (Thermo Fisher Scientific) for 1 h at 4 °C and immunoprecipitations were performed with 30 μL of Pierce Anti-HA Agarose (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... and the supernatant was applied to a 5 mL HisPur Ni-NTA resin (Thermo Fisher Scientific) equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: ... The 400□μl slurry of HisPur™ Ni-NTA agarose resin (cat. no. 88221, Thermo fisher) was washed with wash buffer (5x ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified cell supernatant containing recombinant mAb was passed over Protein A Agarose resin (Thermo Fisher Scientific). Protein A resin was extensively washed with 25 mM Phosphate pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... 30 μg of biotinylated protein were mixed with 60 μL of streptavidin agarose resin (Thermo Fisher) and rotated (end-over-end ...
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bioRxiv - Biochemistry 2020Quote: ... PARL was purified from GFP and TEV using HisPur™ Ni-NTA agarose resin (ThermoFisher, USA). Flow-through was collected and concentrated using a 10 000 MWCO concentrator (Millipore ...
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bioRxiv - Synthetic Biology 2021Quote: The second spent media pools were batch-bound to 5 mL of HisPur NiNTA resin (ThermoFisher) at 4°C for 1 hour before protein-bound resin was pelleted through centrifugation at 3000 r.c.f ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then desalted over Oligo R3 reverse-phase resin (Thermo Scientific, Cat#1-1339-03).
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bioRxiv - Immunology 2021Quote: ... Monoclonal antibodies were purified using protein A resin columns for IgG and CaptureSelect beads (Thermo Scientific) for DH1017.IgM following manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... media was collected and His tagged proteins were bound using HisPur Ni-NTA Resin (ThermoFisher; 88221) in the presence of Calbiochem’s EDTA-free protease inhibitor cocktail 1:1000 (MilliporeSigma ...
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bioRxiv - Cell Biology 2021Quote: ... followed by 5 minutes incubation with 20 μl streptavidin agarose resin (Thermo Fisher Scientific, Waltham, USA) at 4°C under gentle rotation ...
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bioRxiv - Immunology 2022Quote: ... and passed over a column containing 1mL of protein A/G resin (Pierce ThermoFisher, catalog #20422). The column was then washed 3 times with 5 column volumes of binding buffer ...
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bioRxiv - Microbiology 2022Quote: ... The clarified supernatant was incubated overnight at 4°C with HisPur Ni-NTA Resin (Thermo Scientific) pre-equilibrated with the lysis buffer ...
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bioRxiv - Biophysics 2022Quote: ... were filled with ( 1 mL | 0.5 mL ) of bed Ni-NTA resin (Qiagen or Thermo Fisher), and equilibrated with three rinses of Wash buffer (at least 30 resin bed volumes ...
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bioRxiv - Immunology 2021Quote: Monoclonal antibody was digested into Fab using papain resin and purified by protein A beads (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... pH8.0) at 8,000 x g post sonication and purified on a HisPur Cobalt Resin (Thermo Scientific). Two separate elutions (5 mL (E1 ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was bound to 5 mL of Nickel-NTA agarose resin (Thermo Scientific, Waltham, MA) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... protease inhibitors were added to clarified supernatant and purified using protein A-agarose resin (ThermoFisher, GA). Bound antibody is eluted with IgG elution buffer into 1/10th volume of 1M Tris-HCl (pH 8.0).
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bioRxiv - Biochemistry 2022Quote: ... The soluble layer (supernatant) was applied to a cobalt-charged (Co-)NTA resin (Thermo Fisher, #88221) pre-equilibrated with binding buffer in a gravity-column set-up ...