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Citations for Thermo Fisher :
501 - 550 of 1284 citations for Fmoc Lys Boc Rink Amide Resin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The clarified lysates were incubated with 150 µL POROS AAV9 resin (Thermofisher, A27353), at 37°C for 90 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Affinity resin was prepared at room temperature: 100 μl Protein G Dynabeads (Invitrogen) were washed 3x in B-ChIP-BL (0.5 mg/ml BSA in PBS) ...
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bioRxiv - Biophysics 2024Quote: ... The lysate was mixed with 2mL HisPur™ Ni-NTA Resin (Thermo Scientific) for 1 hour before being loaded onto a gravity column ...
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bioRxiv - Biochemistry 2024Quote: ... Resultant supernatants were loaded on 1 mL equilibrated HisPur Ni-NTA resin (ThermoFisher) in gravity flow columns and washed with 15 mL of IMAC-A buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Binary CDK2:CCNE2 protein complexes were purified using nickel ProBond resin (Life Technologies). N-terminal poly-histidine tag was subsequently cleaved off CCNE2 using TEV protease ...
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bioRxiv - Biochemistry 2023Quote: ... HisPur Ni-NTA Resin (#TS-88222) was purchased from Thermo Scientific (Waltham, MA). Alexa Fluor 647-conjugated Annexin V (#A23204 ...
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bioRxiv - Microbiology 2023Quote: ... 0.45 µm filtered and incubated with CaptureSelect™ C-tagXL resin (Thermo Scientific). Beads were washed using 10 column volumes of either PBS or TBS (25 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... The cell-free supernatant was incubated overnight with either protein A resin (ThermoFisher) for IgG1 or LambdaFabSelect or KappSelect resin (Cytvia ...
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bioRxiv - Microbiology 2023Quote: ... The filtered supernatant was incubated with HisPur™ Ni-NTA Resin (Thermo Fisher) (500 µL/50 mL lysate ...
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bioRxiv - Molecular Biology 2023Quote: ... stage tips filled with 4 μl of Poros Oligo R3 resin (Thermo Fisher). Bound peptides were eluted sequentially with 30% ...
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bioRxiv - Neuroscience 2023Quote: ... with a POROS™ CaptureSelect™ AAV Resin (Thermo Fisher Scientific, Oxford, UK) column ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was run over 5 ml of cobalt resin (Thermo Fisher Scientific) equilibrated with lysis buffer + 0.05% DDM + 20 mM imidazole in a gravity flow column ...
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bioRxiv - Biochemistry 2022Quote: ... and was then incubated in batch with HisPur Ni-NTA resin (Thermo Scientific). Resin-bound Gβ1γ2 was washed extensively ...
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bioRxiv - Biochemistry 2023Quote: ... The His-tagged protein was purified using HisPur Cobalt Resin (Thermo Fisher Scientific) in 20 mM Hepes (pH 7.3) ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was mixed in batch with 5 mL Ni-NTA resin (ThermoFisher), equilibrated in SB ...
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bioRxiv - Cancer Biology 2023Quote: ... the collected supernatant was incubated NeutrAvidin Plus UltraLink Resin (Cat. #53151, Thermo Scientific) for 4 hours at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... mNG purification was performed using HisPur™ Ni-NTA Resin (Thermo Scientific, 88221) using batch method specified by manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... pre-cleared with Protein A magnetic resin (Thermo Fisher Scientific catalog number 88846) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Clarified lysate was mixed with 2mL of Cobalt resin (Thermo Fisher Scientific PI89965), column was washed with 20 column volumes of lysis buffer and then eluted with 3ml of elution buffer (150mM KCl ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were purified using of HisPur Ni-NTA resin (Thermo Fisher Scientific, 88222), followed by size exclusion FPLC (HiLoad 16/60 Superdex 200 ...
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bioRxiv - Biophysics 2023Quote: ... protein digests were mixed with 1 mL HisPur Ni-NTA resin (ThermoFisher Scientific) and placed on a rotating mixer maintained at 4 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Solubilized Gβ1γ2 heterodimer was then incubated with HisPur Ni-NTA resin (Thermo Scientific) in batch ...
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bioRxiv - Immunology 2024Quote: ... 100 μl of POROSTM CaptureSelectTM IgM Affinity Matrix packed resin (Thermo Fisher Scientific) was washed thrice with 5 column volume (CV ...
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bioRxiv - Plant Biology 2024Quote: ... Protein G-agarose resin pretreated with mouse monoclonal anti-GFP (Thermo Scientific, A11120) overnight was incubated with clarified supernatant for 2 h at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... Nickel nitrilotriacetic acid resin (HisPur™ Ni-NTA) was purchased from Thermo Scientific. CaCl2 was purchased from EMD Millipore Corporation ...
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bioRxiv - Immunology 2024Quote: ... The soluble fraction was passed through a 1 mL glutathione resin (Thermo Scientific) column and the column was washed with 40 mL of GST purification buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... were incubated with 25 μl CaptureSelect C-tag affinity resin (Thermo Fisher Scientific) in C-tag wash buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Plant Biology 2024Quote: ... Both resins were packed into PierceTM centrifugation columns (Thermo Fisher Scientific, Waltham, USA) before use ...
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bioRxiv - Molecular Biology 2024Quote: ... Clarified lysate was first sent through the HispurTM Ni-NTA resin (Thermo Scientific) column (∼ 20 ml bed volume for 60-70 g of the pellet ...
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bioRxiv - Immunology 2024Quote: ... Capped mRNA was purified by reverse phase chromatography using POROS™ resin (ThermoFisher) followed by tangential flow filtration (TFF) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were further desalted using Oligo™ R3 reversed-phase resin (Thermo Scientific) and eluted twice in 50μl of 30% aqueous ACN containing 0.1% FA ...
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bioRxiv - Molecular Biology 2024Quote: ... 600 µl PierceTM high-capacity streptavidin agarose Resins beads (Thermo Fisher Scientific, 20359) were first equilibrated by washing three times with affinity purification (AP ...
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bioRxiv - Molecular Biology 2024Quote: ... Nanobody-containing supernatant was incubated with 2.5 mL of Ni-NTA Resin (Thermofisher) and 0.01% NaN3 overnight at 4°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... IgGs were then crosslinked to rProtein A resin by adding BS3 (Thermo Scientific™ ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were incubated with 2 mL Pierce Glutathione Agarose resin (Thermo Fisher Scientific) for 1 hour at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... followed by affinity chromatography using POROSTM Oligo (dT)25 Affinity Resin (Thermo Scientific). Ultrafiltration/diafiltration (UF/DF ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatant containing soluble protein was purified using HisPur Ni-NTA resin (Thermo Scientific). The His6-tag was cleaved using tobacco etch virus (TEV ...
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bioRxiv - Biochemistry 2024Quote: ... Fc domains were removed from the samples using CaptureSelect multispecies Fc resin (ThermoFisher). Fabs were further purified by SEC ...
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bioRxiv - Biochemistry 2024Quote: ... The His-tagged protein was purified using HisPur Cobalt Resin (Thermo Fisher Scientific) in 20 mM Hepes (pH 7.3) ...
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bioRxiv - Biochemistry 2024Quote: ... supernatant was harvested and IgG was purified using Protein A resin (Thermo Fisher). Fabs were generated by addition of 1:2000 wt/wt Endoproteinase Lys-C (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... Supernatant was incubated with Ni-NTA beads (HisPur™ Ni-NTA Resin, Thermo Scientific) pre-equilibrated in buffer containing 8M urea ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were purified by tandem affinity chromatography using Glutathione Agarose resin (Thermo Fisher Scientific) followed by Ni-NTA resin ...
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bioRxiv - Microbiology 2021Quote: ... Crude extracts in Bugbuster reagent were purified using HisPur Ni-NTA resin (Thermo Scientific) and run through a Poly-Prep Chromatography Column (BioRad ...
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bioRxiv - Microbiology 2021Quote: ... and purification was conducted using gravity flow over nickel affinity resin (Thermo-Fisher Scientific) as previously described (North et al. ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was incubated with Ni-Sepharose beads (HisPur Ni-NTA resin, Thermofisher) at a final concentration of 2 ml of beads (packed ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting supernatant was mixed with 2mL HisPur™Ni-NTA Resin (Thermo Scientific) for 1 hour before being loaded onto a gravity column ...
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bioRxiv - Biochemistry 2020Quote: ... the lysate was loaded onto a column containing HisPurTM Ni-NTA Resin (Thermo scientific) for immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was then mixed with 8 mL HisPurTM Ni-NTA Resin (Thermo Scientific), and gently stirred at 4 for two hours to allow CtBP to bind to the resin.
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bioRxiv - Immunology 2021Quote: ... The flowthrough was then purified over CaptureSelect™ IgG-CH1 affinity resin (ThermoFisher Scientific), and bound Fab fragments eluted with 200 mM acetic acid with 50 mM NaCl (pH 3.5 ...
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bioRxiv - Immunology 2021Quote: ... ACPA were affinity isolated from 25µl serum samples using NeutrAvidin Plus resin (Thermo Scientific) coupled with 0.1µg/µl CCP2-biotin followed by IgG capturing using FcXL affinity beads (Thermo Scientific) ...