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Citations for Thermo Fisher :
801 - 850 of 1284 citations for Fmoc Lys Boc Rink Amide Resin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and FL Spike trimers from hCoVs polypeptide antigens) were purified using HisPur™ Ni-NTA resin (ThermoFisher). Cell supernatants were diluted with 1/3rd volume wash buffer (20 mM imidazole ...
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bioRxiv - Immunology 2021Quote: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermo Fisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS.51 S1-S2-His (referred as S ...
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bioRxiv - Cell Biology 2020Quote: ... The protease was removed using His-Pur™ Ni-NTA agarose resin (Thermo Fisher Scientific, Waltham, MA). Gle1152-360 was further purified by a 30% ammonium sulfate precipitation ...
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bioRxiv - Immunology 2021Quote: The RAM2 Fabs were generated by papain digestion and purified using Immobilized papain agarose resin (Thermofisher Scientific) following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Insoluble material was pelleted by ultracentrifugation and the supernatant bound to HisPur™ cobalt resin (ThermoFisher, USA) by gravity flow-through column ...
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bioRxiv - Plant Biology 2020Quote: ... The GST and GST-XIK fusion proteins were extracted with Pierce Immobilized Glutathione resin (Thermo Fisher Scientific) with the protein interaction buffer (20 mM Hepes-KOH pH7.2 ...
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bioRxiv - Microbiology 2020Quote: ... The cleared lysate was incubated for 2 h at 4 °C with Ni-NTA agarose resin (Invitrogen) under slight agitation ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant forms of SyAM in culture supernatant were isolated by protein A affinity resins (MabCapture, ThermoFisher Scientific) using Pierce gentle Ag/Ab elution buffer (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2022Quote: For purification of the fusion proteins by His Pur™ Ni-NTA Resin (Thermo Scientific, Beijing, USA), the 250 mL IPTG-induced bacterial solution was centrifuged at 4 °C at 12000 rpm for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... His-tagged proteins were purified using HisPur™ Ni-NTA resin (Thermo Fisher Scientific Cat. Nr. 88222) according to manufacturer’s instructions (batch protocol) ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was subjected to 1.5 h of incubation with HisPur Ni-NTA Resin (Thermo Fisher Scientific) at 4°C for immobilised-metal affinity chromatography (IMAC) ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was then co-incubated with 1 ml of HisPur™ Ni-NTA Resin (Thermo Scientific) for 2 h at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... and applied to a gravity column with 5 mL of HisPur Ni-NTA resin (Thermo Fisher Scientific) prepared in equilibration buffer (40mM Tris [pH 7.4]) ...
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bioRxiv - Microbiology 2020Quote: ... and then conjugated to IgG resin using the Pierce Protein A IgG Plus Orientation Kit (Thermo Fisher), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... His-ubiquitin conjugates were eluted by boiling resin in 2x LDS sample buffer (Thermo Fisher Scientific, NP0008) supplemented with 200 mM DTT and 200 mM imidazole HCl ...
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bioRxiv - Biochemistry 2021Quote: ... and transferrin using the High Select Top 14 Abundant Protein Depletion Camel Antibody Resin (Thermo Fisher Scientific). On the day of depletion ...
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bioRxiv - Immunology 2022Quote: ... The gB Domain I and Domain I+II were purified using Nickel-NTA resin (Thermo Fisher Scientific), and the gB Domain II was purified with lectin resin (VWR.)
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bioRxiv - Cell Biology 2023Quote: CRB3 peptide pull-down assays: 150 µL pelleted streptavidin beads (streptavidin agarose resin, ThermoFisher Scientific, Courtaboeuf, France) were coated with 2mg of a biotinylated peptide mimicking the cytoplasmic part of CRB3A (amino-acids 90 to 120 ...
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bioRxiv - Molecular Biology 2023Quote: ... The unbound fraction was removed by transferring the resin to Pierce Micro-Spin columns (Thermo Fisher Scientific), and centrifugation at 100 x g for 15 s at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 85,000 RCF and incubated with HisPur Ni-NTA resin (Thermo Scientific) for 15 min ...
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bioRxiv - Immunology 2023Quote: ... Filtered supernatants containing His-tagged proteins were passed slowly through HisPur Ni-NTA Resin (Thermo Fisher 88221) beads in columns ...
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bioRxiv - Cell Biology 2023Quote: Lysates were then incubated with rotation for 2 hours (at 4°C) with Talon cobalt resin (Thermofisher). After incubation ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were run over a gravity column containing 4 mL of fresh HisPur cobalt resin (Thermo Scientific) equilibrated in wash buffer (50 mM NaPO4 ...
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bioRxiv - Molecular Biology 2022Quote: ... or mouse IgG-1κ (for negative control) conjugated to Protein A/G UltraLink Resin (Thermo Fisher Scientific) at room temperature for 2 h with gentle rotation ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were then equilibrated and loaded into a tube with HisPur Ni-NTA resin (Thermo Scientific, 88221) per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... and SLC35A1 KO HAP1 cells was purified by HisPur™ Ni-NTA Resin (Thermo Fisher Scientific, US). The nickel resin was eluted by 300 mM imidazole ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was then loaded onto 3 mL of pre-equilibrated Ni-NTA resin (Thermo Fisher Scientific). The column was washed with 30 mL of lysis buffer followed by 90 mL of wash buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Synthetic Biology 2023Quote: All RBD antigens were His-tagged and purified using HisPur Ni-NTA resin (Thermo Fisher Scientific, 88222). Cell supernatants were diluted with 1/3 volume of wash buffer (20 mM imidazole ...
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bioRxiv - Plant Biology 2024Quote: ... the supernatant was loaded onto a column containing 1 mL of Ni-NTA resin (Invitrogen, Waltham, MA) that had been previously equilibrated with 1 column volume of lysis buffer ...
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bioRxiv - Immunology 2024Quote: ... The flow-through was collected and Fabs were purified using CaptureSelectTM IgG-CH1 affinity resin (ThermoFisher Scientific). 200 mM acetic acid (pH 3.5) ...
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bioRxiv - Bioengineering 2022Quote: ... all syntheses were cleaved from resin with 5 mL of 92.5:2.5:2.5:2.5 trifluoroacetic acid (>95%, Fisher Scientific):DI water:triisopropylsilane (98% ...
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bioRxiv - Biochemistry 2022Quote: ... and the resulting supernatants were incubated with either Pierce Anti-c-Myc-Agarose resin (Thermo Fisher Scientific), Strep-TactinXT 4Flow (iba ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were then incubated with rotation for 2 hours (at 4°C) with Talon cobalt resin (Thermofisher). After incubation ...
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bioRxiv - Immunology 2022Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher Scientific cat.# 88221). After washing by wash buffer (25 mM Imidazole ...
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bioRxiv - Microbiology 2023Quote: ... The cell lysate-resin mixture was added to a disposable plastic column (1×5 cm) (Thermo Scientific) and allowed to settle ...
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bioRxiv - Genetics 2024Quote: ... The Ni-resin was collected by centrifugation and placed into a 10 mL polypropylene column (Thermo Scientific) and washed with 100 column volumes of wash buffer (10 mM Tris/HCl pH 8.0 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The supernatant containing DDM-solubilized DmPglB was mixed with 0.8 mL of HisPur Ni-NTA resin (ThermoFisher) equilibrated with Buffer B supplemented with protease inhibitor cocktail and incubated rotating for 24 h at 4 ⁰C ...
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bioRxiv - Immunology 2024Quote: Recombinant H3 (SinoBiological, 40859-V08H1) was immobilized on N-hydroxysuccinimide (NHS)–activated agarose resin (Thermo Fisher, 26197) by overnight rotation at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Immunoprecipitation of TeeVax3-specific antibodies was performed by coupling TeeVax3 antigen to AminoLink Plus Coupling Resin (ThermoFisher). 8 mg of TeeVax3 antigen in 0.1 M sodium phosphate ...
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bioRxiv - Cancer Biology 2024Quote: The BG4 protein was purified from the cell lysate using HisPur cobalt resin (2.5 mL, Thermo Scientific). The resin was washed with Milli-Q water (12.5 mL ...
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bioRxiv - Biochemistry 2024Quote: ... Antibody-bound protein was mixed with equilibrated resin from 25 µL protein A-conjugated dynabeads (Invitrogen, 1011D) for 1 hour at 4°C rotating ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were pelleted and the supernatant was filtered and applied to a CaptureSelect IgG-CH1 resin (ThermoFisher). The resin was washed with 10 column volumes of 20 mM Hepes pH 7.4 ...
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bioRxiv - Biophysics 2024Quote: ... pH 7.2) and then loaded onto a column packed with POROS CaptureSelect AAVX resin (Thermo Fisher Scientific) at a flow rate of 300 cm/hr ...
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bioRxiv - Biochemistry 2024Quote: ... affinity-purified from serum using the recombinant ELOF1 coupled to AminoLink™ Coupling Resin (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The cleared extract was incubated 20 minutes with 2 ml pre-equilibrated Ni-NTA resin (Thermo Scientific) and washed extensively with a buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Genetics 2024Quote: ... 5 mM beta- mercaptoethanol) and applied to the 5 ml HisPurTM Ni-NTA resin (Thermo Fisher Scientific) equilibrated with the same binding buffer ...
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bioRxiv - Biophysics 2021Quote: ... coli BL21 (DE3) from New England Biolabs and purified using HisPur™ Ni-NTA Resin from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: Samples were incubated with 100 μL (1:10 ratio of bead suspension:protein) of NeutrAvidin agarose resin (Thermo Scientific 29201 ...
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bioRxiv - Cell Biology 2020Quote: The supernatant was incubated with equilibrated Pierce™ NeutrAvidin™ Plus UltraLink™ Resin (Thermo Fisher Scientific, 53151), followed by incubation for 30–45 min at 4 °C on a wheel ...
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bioRxiv - Molecular Biology 2021Quote: ... The soluble fraction was loaded on 1.5 ml of HisPur™ Ni-NTA Resin (Thermo Fisher scientific Inc.) and incubated 1 hour at 4°C on rotation ...