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Citations for Thermo Fisher :
951 - 1000 of 1284 citations for Fmoc Lys Boc Rink Amide Resin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: The recombinant abGFP4 nanobody against GFP with a C-terminal cysteine was covalently coupled to SulfoLink resin (Thermo Scientific) consisting of cross-linked agarose beads with iodoacetyl groups ...
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bioRxiv - Cell Biology 2022Quote: ... The 100 µm capillary column was packed with 35 cm of Accucore 150 resin (2.6 μm, 150Å; ThermoFisher Scientific). The scan sequence began with an MS1 spectrum (Orbitrap analysis ...
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bioRxiv - Molecular Biology 2023Quote: ... Volume containing equal protein amounts of each sample were incubated with high capacity Neutravidin Agarose Resin (Thermo Scientific, 29204) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... the expressed protein was extracted from the supernatant using PierceTM High-Capacity Ni-IMAC Resin (Thermo Scientific, Waltham, MA) and eluted using lysis buffer supplemented with 300 mM Imidazole pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... 140 µg of protein were incubated with 12 µl of Pierce anti-HA magnetic bead resin (Thermo Fisher Scientific) on a rotator wheel at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... and buffer exchange was performed with a 7 kDa molecular weight cut-off size exclusion resin (Thermo Fisher Scientific) to cryo-EM buffer (10 mM Tris at pH 7.6 and 20 mM NaCl).
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bioRxiv - Biophysics 2022Quote: ... before removing 6μL of the input for SDS-PAGE analysis and adding 40μL of prepared HisPur Ni-NTA resin (Thermo Scientific). Samples were incubated for 60min on a rotating wheel at 4°C before flow through was collected by centrifugation for 1min at 1000 rcf ...
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bioRxiv - Molecular Biology 2023Quote: ... The 100 µm capillary column was packed with 35 cm of Accucore 150 resin (2.6 μm, 150Å; ThermoFisher Scientific) at a flow rate of 565 nL/min ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified cell lysates were loaded onto a column containing equilibrated immobilized nickel affinity chromatography resin (Thermo Fisher Scientific) and eluted using a linear imidazole gradient from 50 mM to 250 mM ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was clarified by filtering through a 0.44 ×G μm PVDF syringe filter and then incubated with 2 mL of Pierce glutathione agarose resin (16100, ThermoFisher) for 2 hr at 4 °C in a 5 mL polypropylene column ...
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bioRxiv - Biochemistry 2024Quote: ... and a 75 μm x 15 cm analytical column with C18 2 μm resins (Acclaim PepMap RSLC, Thermo Scientific). The nanoLC gradient was 3−35% solvent B (A = H2O with 0.1% formic acid ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was mixed with 1 ml HisPur Ni-NTA washed resin slurry (Cat. No. 88222, Thermo Fisher Scientific) for at least 2 h at 4 °C under continuous rotation ...
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bioRxiv - Microbiology 2024Quote: ... ExsAHis-containing bacterial lysate was cleared by centrifugation and subjected to purification by HisPur Ni-NTA Resin (Thermo Scientific) as per the manufacturer’s instruction.
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bioRxiv - Plant Biology 2024Quote: ... NbISIC1 and its mutant were induced with 0.1 mM IPTG for 16 h at 12℃ and purified using Ni-NTA agarose resin (ThermoFisher). Proteins were concentrated to 50 μM in pull-down buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2024Quote: ... The clarified protein samples were incubated with NeutrAvidin® Agarose resin (50 µL per 1 mg protein, Thermo Scientific) for 2 hours at RT ...
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bioRxiv - Plant Biology 2024Quote: ... GST and GST-NbSTIR1 were induced with 0.4 mM IPTG for 4 h at 37℃ and purified using Glutathione agarose resin (ThermoFisher). NbISIC1 and its mutant were induced with 0.1 mM IPTG for 16 h at 12℃ and purified using Ni-NTA agarose resin (ThermoFisher) ...
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bioRxiv - Cell Biology 2024Quote: ... Polyclonal SYP-5 pS541 antibodies were purified by passing the immune serum through SulfoLink Coupling Resins (Thermo Scientific, 44995) coupled to the non-phosphopeptide (CEEGDADQSIWGSDD ...
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bioRxiv - Biochemistry 2024Quote: ... The 100 µm capillary column was packed with 35 cm of Accucore 150 resin (2.6 μm, 150Å; ThermoFisher Scientific) at a flow rate of 360 nL/min ...
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bioRxiv - Biochemistry 2024Quote: ... then incubated with rocking for 1 h at 4 °C with 1 mL HisPur Ni-NTA resin (ThermoFisher Scientific) pre-equilibrated in SEC buffer ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were lysed and the proteins were purified by His tag purification (HisPur Ni-NTA Resin, Thermo Scientific), followed by concentration and buffer exchange into Exchange buffer (50mM HEPES ...
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bioRxiv - Cell Biology 2024Quote: ... The 100 µm capillary column was packed with 35 cm of Accucore 150 resin (2.6 μm, 150Å; ThermoFisher Scientific) at a flow rate of 340 nL/min ...
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bioRxiv - Cell Biology 2024Quote: ... the supernatant was filtered through a 0.45 µM filter and incubated in batch mode with His-Pur Ni-NTA resin (ThermoFisher) + 20 mM imidazole (3 h ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were separated on a 100 μm inner diameter microcapillary column packed with 35 cm of Accucore C18 resin (2.6 μm, 150 Å, ThermoFisher). For each analysis ...
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bioRxiv - Biochemistry 2021Quote: ... Sample was spun at 29,000 RCF at 4°C and supernatant was incubated with rocking with Pierce Glutathione Agarose Resin (Thermo Fisher) for 2-3 hours ...
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bioRxiv - Neuroscience 2020Quote: ... EV-antibody complexes were incubated with 30 μL of washed Pierce™ Streptavidin Plus UltraLink™ Resin (Thermo Fisher Scientific) for 1 hour at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Insoluble material was removed by centrifugation at 10,000xg at 4°C for 10min and the supernatant was incubated with 50ul NeutrAvidin agarose resin (Thermo Scientific) with gentle mixing overnight at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... supernatants were harvested and filtered with a 0.22 µm membrane.The His-tagged proteins were purified with the HisPur Ni- NTA Resin (Thermo Fisher, 88222). After three columns of washing with 25 mM Imidazole (pH 7.4) ...
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bioRxiv - Biochemistry 2020Quote: ... recombinant Bmh1 and Hst2 isoforms (0.3 mg each) were mixed with 20 μl 50:50 slurry of Avidin Agarose Resin (Fisher Scientific) in 80 µl of Bmh1 SEC buffer (20 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2020Quote: ... The antibody was purified by affinity chromatography using the peptide immobilized on an agarose resin via the Sulfo-Link chemistry according to the manufacturer’s instruction (Thermo Scientific). The antibodies were eluted sequentially with a 0.2 M glycine solution at pH 3 and pH 2.5 ...
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bioRxiv - Biochemistry 2021Quote: The Fab fragment of the ch14D5 antibody was prepared from the antibody using Immobilized Papain-agarose resin (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2021Quote: ... supernatants were harvested and filtered with a 0.22 μm membrane.The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher, 88222). After three columns of washing with 25 mM Imidazole (pH 7.4) ...
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bioRxiv - Biochemistry 2020Quote: ... with a N-terminal 8x His tag was expressed from the pSJ2 vector (84) in BL21 E.coli and purified by Ni-NTA resin (Thermo Fisher). Full-length human Src kinase (WT or K298M ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected with a plasmid coding for SARS-CoV-2 RBD or ACE2-Fc and were purified on Ni-NTA resin (Invitrogen) or Protein A resin (Cytiva) ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were centrifuged for 10 minutes at 10,000 rpm and the clarified lysates loaded onto a glutathione resin and purified by batch purification according to the manufacturer’s protocol (ThermoFisher, PI16101). Purified proteins were then dialyzed against Lysis Buffer to remove GSH and quantified using a Bradford assay per the manufacturer instructions (BioRad ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were injected onto a PepMap100 trap column (0.3 x 5 mm packed with 5 μm C18 resin; Thermo Scientific), and peptides were separated by reversed phase HPLC on a BEH C18 nanocapillary analytical column (75 μm i.d ...
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bioRxiv - Synthetic Biology 2021Quote: ... Clarified lysates containing His6x-tagged proteins were subjected to gravity-flow Ni2+-affinity purification using HisPur Ni-NTA Resin (ThermoFisher) following manufacturer’s protocols ...
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bioRxiv - Cell Biology 2020Quote: ... per 1 mg of total protein were added along with 30 μL washed protein A/G UltraLink Resin (53133, ThermoFisher) and rotated overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... which were further separated on an in-line 150 mm × 75 µm reversed phase analytical column packed in-house with XSelect CSH C18 2.5 µm resin (Waters) using an UltiMate 3000 RSLCnano system (ThermoFisher Scientific) at a flow rate of 300 nL/min ...
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bioRxiv - Systems Biology 2022Quote: ... The 100 μm capillary column was packed in-house with 35 cm of Accucore 50 resin (2.6 μm, 150Å; ThermoFisher Scientific). The mobile phase was 5% acetonitrile ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation and the protein was purified by nickel affinity chromatography (Thermo Scientific HisPur Ni-NTA Resin). After applying cleared lysate to the resin on-column ...
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bioRxiv - Biophysics 2020Quote: ... The proteins in the periplasmic extract were released by osmotic shock and purified by immobilized metal affinity chromatography (IMAC) using Ni-NTA resin (Invitrogen) in TBS buffer (20 mM Tris ...
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bioRxiv - Microbiology 2020Quote: We purified polyclonal IgG from human plasma samples using Pierce™ Protein G Ultra Link™ Resin (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was spun down at 19,000 rpm for 30 minutes and the supernatant was added to Ni-NTA metal affinity resin (Thermo Scientific) and left to incubate at 4 °C for 1 hr ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were embedded in epoxy resin and sectioned (ultrathin sectioning, 60 nm thick) then visualised on a Tecnai T20 transmission electron microscope (ThermoFisher) operating at 120 kV.
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bioRxiv - Biochemistry 2020Quote: ... 20,000 rpm 30 minutes) and the supernatant was incubated and passed over a HisPur™ Cobalt Resin (ThermoFisher Scientific 89965) in gravity columns ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 S2P spike trimer proteins of the D614G and Omicron subvariants were generated by transfecting Expi293 cells with the S2P spike trimer-expressing constructs using 1 mg mL-1 polyethylenimine (PEI) and then purifying from the supernatants five days post-transfection using Ni-NTA resin (Invitrogen) according to the manufacturer’s instructions17.
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bioRxiv - Cell Biology 2020Quote: ... The 100 μm capillary column was packed with 30 cm of Accucore 150 resin (2.6 μm, 150Å; Thermo Fisher Scientific). Mobile phases were 5% ACN ...
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bioRxiv - Microbiology 2020Quote: ... which was equipped with a 75 µm x 2 cm trap column packed with C18 3µm bulk resins (Acclaim PepMap 100, Thermo Scientific) and a 75 µm x 15 cm analytical column with C18 2 µm resins (Acclaim PepMap RSLC ...
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bioRxiv - Biochemistry 2021Quote: ... Each super-fraction was further separated on an in-line 150 mm x 75 μm reversed phase analytical column packed in-house with XSelect CSH C18 2.5 μm resin (Waters) using an UltiMate 3000 RSLCnano system (ThermoFisher Scientific), at a flow rate of 300 nL/min ...
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bioRxiv - Bioengineering 2021Quote: ... equilibrated with two resin-beds of equilibration buffer was added 1:20 lysate and end-over-end shaker (Thermo Fisher) incubated at 4C overnight ...