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Citations for Thermo Fisher :
551 - 600 of 1284 citations for Fmoc Lys Boc Rink Amide Resin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... proteins were purified by immobilized metal affinity chromatography (IMAC; HisPur NiNTA resin, ThermoFisher Scientific). The His-tag was removed by Tobacco Etch Virus (TEV ...
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bioRxiv - Microbiology 2021Quote: ... Lysate was through Talon gravity columns loaded with HisPur™ Cobalt Resin (Thermo Scientific), washed with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... -F3 (80 nM each) were incubated with pre-equilibrated Ni-NTA resin (Thermofisher Scientific) in buffer F (50 mM Tris-HCl ...
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bioRxiv - Bioengineering 2021Quote: Fusion proteins were purified using Ni-NTA resin according to manufacturer’s instructions (Thermofisher, R90115). Briefly ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation and passed over a nickel resin (Thermo Scientific), which was subsequently washed with ~10 column volumes of wash buffer containing 25 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The clarified lysate was applied to a HisPur™ Ni-NTA Resin (ThermoFisher Scientific) column ...
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bioRxiv - Biochemistry 2020Quote: ... The clarified lysate was applied to a HisPur™ Ni-NTA Resin (ThermoFisher Scientific) column ...
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bioRxiv - Biophysics 2021Quote: ... We added 2 ml Ni2+-NTA slurry (HisPur Ni-NTA resin, Thermo Fisher Scientific) in binding buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were purified by tandem affinity chromatography using glutathione agarose resin (Thermo Fisher Scientific) followed by Ni-NTA resin ...
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bioRxiv - Biophysics 2021Quote: ... and purified using HisPur™ Ni-NTA Resin (Thermo-Fisher Scientific, Waltham, MA, USA). Protein expression was induced with 1 mM IPTG at OD600 = 0.6 overnight at 16°C ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was mixed with the HisPur Ni-NTA Resin (Thermo Fisher Scientific) at + 4 °C for 2 h ...
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bioRxiv - Biochemistry 2020Quote: ... Supernatant was then passed through 1 mL settled HisPur™ cobalt resin (ThermoFisher, USA) by gravity flow to allow binding of FRET- PINK1-His to the resin ...
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bioRxiv - Systems Biology 2020Quote: ... in-house packed tips containing 80 μl Streptavidin Plus UltraLink resin (Thermo Fisher Scientific) were hooked to a Versette liquid-handling system (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The resulting supernatant was then bound to HisPur™ Ni-NTA resin (ThermoFisher Scientific) for 90 minutes at 4°C and then applied to a gravity column ...
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bioRxiv - Microbiology 2021Quote: ... The antiserum was then purified using an AminoLink Plus coupling resin (Thermo Scientific, Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Clarified lysate was bound to 0.5 mL of HisPur Ni-NTA Resin (Thermo Scientific). The resin was then washed with 100 mL of buffer A and bound proteins were eluted in Buffer B [20 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleared lysate was incubated with 1mL His-Pur nickel-NTA resin (Thermo Fisher) with rotation at 4 °C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... Supernatant was loaded onto a gravity flow column containing HisPurTM Cobalt Resin (Thermo Scientific). Column was washed 5 CV with buffer containing 100 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was clarified by centrifugation and passed over Nickel-NTA resin (Thermo Scientific), which was subsequently washed with ~10 column volumes of wash buffer containing 25 mM Tris ...
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bioRxiv - Immunology 2022Quote: ... Endotoxin was removed using Pierce High Capacity Endotoxin Removal Resin (Fisher Scientific, Waltham, MA). Endotoxin was quantified using a Pierce LAL chromogenic Endotoxin quantitation kit (Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... All proteins were initially purified using HisPur Ni-NTA affinity resin (Thermo Fisher Scientific) using Ni-NTA buffer supplemented with 25 mM imidazole during wash and 250 mM imidazole during elution ...
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bioRxiv - Biochemistry 2022Quote: ... A cleared S100 lysate was mixed for 3 h with Ni-NTA resin (Invitrogen) equilibrated with Buffer B (50 mM Tris-HCl (pH 8.0 at 4 °C) ...
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bioRxiv - Immunology 2022Quote: ... The resin was then washed with Pierce IgG Elution buffer (ThermoFisher Scientific, catalog #21004) to remove any E1D1 antibody that was not crosslinked to the resin ...
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bioRxiv - Immunology 2022Quote: ... The proteins were captured from the clarified cell culture using TALON resin (ThermoFisher Scientific) and eluted with imidazole ...
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bioRxiv - Genomics 2022Quote: ... The 15N-GST-H4 peptide fusion was purified on glutathione agarose resin (Thermofisher Scientific), cleaved with PreScission Protease (16 hr incubation at 4°C) ...
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bioRxiv - Biochemistry 2022Quote: ... the proteasomes were eluted from the magnetic resin by incubating with TEV protease (Invitrogen) in buffer B containing 1 mM ATP for 1 h 30 min at 30 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Solubilized Gβ1γ2 heterodimer was then incubated with HisPur™ Ni-NTA resin (Thermo Scientific) in batch ...
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bioRxiv - Microbiology 2020Quote: ... Fabs were expressed in ExpiCHO cells and purified using CaptureSelect CH1-XL resin (ThermoFisher) and followed by size exclusion chromatography ...
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bioRxiv - Neuroscience 2021Quote: The pulldown assay was performed using neutravidin coated agarose resin (ThermoFisher Scientific, Waltham, MA). 100 μL bead volume (BV ...
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bioRxiv - Synthetic Biology 2022Quote: All antigens were His-tagged and purified using HisPur™ Ni-NTA resin (ThermoFisher). Cell supernatants were diluted with 1/3rd volume wash buffer [20 mM imidazole ...
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bioRxiv - Immunology 2022Quote: ... Capped mRNA was purified by reverse phase chromatography using POROS™ resin (Thermo Fisher) followed by tangential flow filtration (TFF) ...
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bioRxiv - Immunology 2022Quote: ... we first covalently conjugated CspZ onto AminoLink Plus Coupling Resin (ThermoFisher Scientific, Waltham, MA) based on the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... then loaded to a gravity column packed with CaptureSelect® IgA resins (Thermo Fisher) pre-equilibrated with 10 mM Na Phosphate and 150 mM NaCl at pH 7.0 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein were purified with Ni-NTA resin according to the manufacturer’s instructions (R90115, Invitrogen). For GST-tagged proteins ...
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bioRxiv - Physiology 2021Quote: ... the entire left Sol muscle was mounted in Cryomatrix embedding resin (Thermo Scientific, USA) and frozen in liquid isopentane that had been chilled to the point of freezing in liquid N2 ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged proteins were purified using HisPur Ni-NTA resin (Thermo Fisher Scientific), at RT ...
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bioRxiv - Biochemistry 2021Quote: ... The clear supernatant was incubated with Ni-NTA affinity resin (Thermo Fisher Scientific, USA). PLpro was eluted by gravity flow using lysis buffer supplemented with 300 mM imidazole and subsequently incubated with TEV protease at a molar ratio of 20:1 in the presence of 1 mM DTT ...
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bioRxiv - Immunology 2021Quote: ... and the protein was purified on His-Pur Ni-NTA resin (Thermo Fisher Scientific) followed by size-exclusion chromatography ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting supernatant was then bound to HisPur™ Ni-NTA resin (ThermoFisher Scientific) for 1 hour at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher). To eliminate nonspecific binding proteins ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was added to tubes containing HisPur NiNTA resin (Thermo Fisher Scientific) and tubes were rocked for 30 minutes at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... the resulting supernatant was added to tubes containing HisPur NiNTA resin (Thermo Fisher Scientific) and tubes were rocked for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were purified by tandem affinity chromatography using glutathione agarose resin (Thermo Fisher Scientific) followed by Ni-NTA resin ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was mixed with 10 µL packed streptavidin agarose resin (Thermo Scientific Cat. #20357) which had been functionalized with biotinylated Alfa-Nb ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated for 30 minutes on ice with His-Pur Ni-NTA resin (Thermo Scientific), packed to a height of approximately 5 cm in polypropylene columns ...
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bioRxiv - Biophysics 2022Quote: ... The dialyzed protein is then cross-linked to AminoLink Plus resin (Thermo Fisher #PI20501) following manufacturer recommended protocols (Fig ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... the resin was separated from supernatant in a magnetic rack (DynaMag-5, Thermofisher, #12321D) and washed with 1 ml of IP buffer for 20 minutes at 4°C ...
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bioRxiv - Immunology 2024Quote: ... purified IgG was bound to Pierce™ Protein A Plus Agarose resin (ThermoFisher Scientific) and washed with 1X PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... the bacteria lysates were incubated with Ni-NTA Agarose resin (Invitrogen®, R901-15) overnight at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant was then processed through a His-tag cobalt resin (ThermoFisher, Waltham, MA, USA) following manufacturer’s protocol ...