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Citations for Thermo Fisher :
7251 - 7300 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... mice were perfused with 30 mL 0.01M phosphate buffered saline (Fisher Scientific, BP2944-100, Pittsburgh, PA), followed by 30 mL 4% paraformaldehyde (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed three times with phosphate-buffered saline (PBS, ThermoFisher Scientific, Waltham MA, 10010-023) and switched to serum-free ...
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bioRxiv - Bioengineering 2023Quote: ... Next, a mixture of glycerol (Gly, AcrosOrganics) and phosphate buffer solution (0.8 mL, Gibco, pH 7.4) preheated to 80 °C ...
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bioRxiv - Microbiology 2023Quote: ... in a 50 µg/mL solution in Dulbecco’s phosphate-buffered saline (DPBS) (Gibco, Thermo Fisher Scientific) for 30 min and washed once with DPBS ...
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bioRxiv - Microbiology 2023Quote: ... in a 50 µg/mL solution in Dulbecco’s phosphate-buffered saline (DPBS) (Gibco, Thermo Fisher Scientific) for 30 min and washed once with DPBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell washes were performed with phosphate buffered saline (PBS) and detachment was done using Trypsin (Gibco). All the cell lines were tested negative for mycoplasma contamination several times during the study.
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bioRxiv - Microbiology 2023Quote: ... Then cells were washed briefly with Phosphate Buffered Saline (PBS, Thermo Fisher Scientific, Waltham, MA, USA) and cultured in infection medium (0.5 % Bovine Serum Albumin (BSA ...
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bioRxiv - Microbiology 2023Quote: ... The resulting cell pellet underwent two washes with 1X phosphate-buffered saline (PBS) sourced from Gibco, USA ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then washed twice with phosphate-buffered saline (PBS) and dissociated using TrypLE Express (ThermoFisher). Cells were then resuspended in PBS and 5% FBS to neutralize the enzymatic reaction ...
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bioRxiv - Bioengineering 2024Quote: ... Stock nanoparticle solutions were diluted with 1X phosphate buffered saline (PBS) (Thermo Fisher Scientific, Cat. # 10010023) to generate desired nanoparticle inputs.
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bioRxiv - Biophysics 2024Quote: ... Vaccine-inoculated cells and control cells were rinsed with phosphate-buffered saline (PBS, Gibco, Carlsbad, CA), and fixed with 4% paraformaldehyde (Sino Biological ...
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bioRxiv - Cancer Biology 2024Quote: Lungs were cleared with a cardiac perfusion of 10 ml sterile phosphate-buffered saline (PBS; Gibco) through the right ventricle ...
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bioRxiv - Microbiology 2024Quote: ... The remaining cell pellet was resuspended in 100 μL of Dulbecco’s Phosphate-Buffered Saline (DPBS) (Gibco). SDS sample loading buffer was added to the supernatant samples and resuspended cells to a final concentration of 1X ...
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bioRxiv - Biophysics 2024Quote: ... then washed in phosphate buffered saline (PBS) and permeabilized with 10μg/ml proteinase K (Invitrogen, Inc.) in PBST (0.1% Tween-20 in PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... Transfection was carried out using the calcium phosphate method for HEK293T cells and Lipofectamine LTX (Invitrogen) or Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ATP hydrolysis was analyzed in continuous reactions using the EnzChek Phosphate Assay Kit (Thermo Fisher Scientific), according to the manufacturer’s manual ...
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bioRxiv - Microbiology 2024Quote: ... Growth on agar plates was harvested with phosphate buffered saline (Catalog # 10010-023, Gibco, Paisley, PA). The bacterial suspensions were pelleted by centrifugation at 10,000 rpm for 15 min at ambient temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and cells were rinsed three times with pre-warmed Dulbecco’s phosphate-buffered saline (DPBS) (Gibco, #14190136). Then ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were washed and homogenized with Dulbecco’s phosphate-buffered saline (DPBS, Gibco, ref.14190-136) containing 0.5% BSA and 3 mg/mL DNAse I grade II (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... injection of a ketamine-xylazine cocktail and transcardially perfused with 1X phosphate-buffered saline (PBS, Invitrogen) and then 4% paraformaldehyde (PFA ...
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bioRxiv - Developmental Biology 2024Quote: First-trimester placental explants were washed in phosphate buffer saline (PBS) followed by DMEM/F12(Gibco) media and divided into smaller pieces under the dissection microscope in sterile conditions and cultured as described previously 20,47 ...
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bioRxiv - Neuroscience 2024Quote: ... The solution is 0.1 mg/mL of PDL in phosphate-buffered saline (PBS) (10010-023, ThermoFisher). 100 μL of the PDL solution is pipetted onto the chip surface ...
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bioRxiv - Bioengineering 2024Quote: ... Stock solutions were prepared with 1x pH-7.4 phosphate buffered saline (PBS) (Thermo Fisher, Waltham, MA) to near maximum solubility ...
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bioRxiv - Bioengineering 2024Quote: ... they were washed with phosphate-buffered saline (PBS) and detached using TrypLE Express (Gibco, United States). The enzymes were neutralized with 5% FBS in DMEM ...
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bioRxiv - Cell Biology 2021Quote: Human LAMP2A was synthesized into pUC57 Plasmid with the sequence: (5’-CAAGTTTGTACAAAAAAGCAGGCTCTCGAGCAatgGTGTGCTTCCGCCTCTTCCCGGTTCCGGGCTCAGGGCTCGTTCTGGTCTGCCTAGTCCTGGGAGCTGTGCGGTCTTATGCATTGGAACTTAATTTGACAGATTCAGAAAATGCCACTTGCCTTTATGCAAAATGGCAGATGAATTTCACAGTACGCTATGAAACTACAAATAAAACTTATAAAACTGTAACCATTTCAGACCATGGCACTGTGACATATAATGGAAGCATTTGTGGGGATGATCAGAATGGTCCCAAAATAGCAGTGCAGTTCGGACCTGGCTTTTCCTGGATTGCGAATTTTACCAAGGCAGCATCTACTTATTCAATTGACAGCGTCTCATTTTCCTACAACACTGGTGATAACACAACATTTCCTGATGCTGAAGATAAAGGAATTCTTACTGTTGATGAACTTTTGGCCATCAGAATTCCATTGAATGACCTTTTTAGATGCAATAGTTTATCAACTTTGGAAAAGAATGATGTTGTCCAACACTACTGGGATGTTCTTGTACAAGCTTTTGTCCAAAATGGCACAGTGAGCACAAATGAGTTCCTGTGTGATAAAGACAAAACTTCAACAGTGGCACCCACCATACACACCACTGTGCCATCTCCTACTACAACACCTACTCCAAAGGAAAAACCAGAAGCTGGAACCTATTCAGTTAATAATGGCAATGATACTTGTCTGCTGGCTACCATGGGGCTGCAGCTGAACATCACTCAGGATAAGGTTGCTTCAGTTATTAACATCAACCCCAATACAACTCACTCCACAGGCAGCTGCCGTTCTCACACTGCTCTACTTAGACTCAATAGCAGCACCATTAAGTATCTAGACTTTGTCTTTGCTGTGAAAAATGAAAACCGATTTTATCTGAAGGAAGTGAACATCAGCATGTATTTGGTTAATGGCTCCGTTTTCAGCATTGCAAATAACAATCTCAGCTACTGGGATGCCCCCCTGGGAAGTTCTTATATGTGCAACAAAGAGCAGACTGTTTCAGTGTCTGGAGCATTTCAGATAAATACCTTTGATCTAAGGGTTCAGCCTTTCAATGTGACACAAGGAAAGTATTCTACAGCTCAAGACTGCAGTGCAGATGACGACAACTTCCTTGTGCCCATAGCGGTGGGAGCTGCCTTGGCAGGAGTACTTATTCTAGTGTTGCTGGCTTATTTTATTGGTCTCAAGCACCATCATGCTGGATATGAGCAATTTTAGGGTACCACCCAGCTTTCTTGTACAAAGTGGACGCGT-3’) Human LAMP2A was subcloned into pLenti6 (Thermo Scientific), a Gateway adapted vector using BP/LR Clonase II (Thermo Scientific) ...
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bioRxiv - Systems Biology 2023Quote: ... and washed again in PBS (3 x 5 min at RT) before being mounted in Prolong Gold (Invitrogen by Thermo Fisher Scientific, Grand Island, NY, USA) and allowed to cure for 48 hours at RT before imaging ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 2 μM Fura-2 acetoxymethyl ester (Fura-2 AM; ThermoFisher Scientific) and 0.01% pluronic acid (Merck ...
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bioRxiv - Developmental Biology 2024Quote: ... while the rest were re-plated onto three matrigel-coated 10 cm dishes (3 replicates / 3 million cells per plate) in N2/B27 media containing 3 µg/mL puromycin (Fisher Scientific; Cat. no. 501532829) to select for the pMK1334 sgRNA vector ...
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bioRxiv - Cancer Biology 2023Quote: ... ITS (5 μg/ml insulin, 5 μg/ml transferrin, and 5 ng/ml sodium selenite, Gibco), and antibiotics (100 U/ml penicillin and 0.1 mg/ml streptomycin) ...
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were assayed using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™, Thermo Fisher Scientific, cat. no. V13154). Cells were plated in triplicate in a 96-well plate (5,000 cell/well) ...
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bioRxiv - Evolutionary Biology 2020Quote: ISH and dFISH were performed as previously described (3, 7, 62) with addition of T1 RNAse treatment (2 u/µl, Thermo Fisher Scientific, Waltham, MA, USA) after probe washing in embryos older than 4 dpf to reduce background staining ...
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bioRxiv - Bioengineering 2021Quote: Human lung fibroblasts (hTERT T1015, abmgood) were used between passages 7-12 and culture medium was changed every 2-3 days (Gibco Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10 v/v% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... The qRT-PCR assays were performed to quantify the amount of SARS-CoV-2 RNA with QuantStudio 3 instrument (Thermo Fisher Scientific, Waltham, MA, USA) using primers and probes described previously8,9 (N2 set or E set).
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were determined using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™, Thermo Fisher Scientific, cat. no. V13154). Cells were plated in triplicate in a 96-well plate (3,000 cell/well in 200 μL of complete medium and cultured under standard conditions for 48 h ...
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bioRxiv - Cell Biology 2022Quote: ... The medium was replaced at day 1 to remove non-adherent cells and afterwards every 2-3 day with DMEM/10% FBS/1% penicillin streptomycin (PS) (Gibco, USA, catalog number 15140-122). These cells were passaged serially when cells reached to 80-100 % confluency or when proliferation essentially ceased by using 0.25% Trypsin/EDTA (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... Hippocampal neurons were seeded onto polyethylenimine-coated 3-cm dishes (2 × 105 cells/dish) and cultured in neurobasal medium (Gibco; Thermo Fisher, Waltham, MA, USA) supplemented with B-27 and 2 mM Glutamax (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... Patient-derived primary cell line GCDX62 was maintained in a 3:1 mixture of Keratinocyte-SFM (KSF; Thermo Fisher Scientific; supplemented with 2% (v/v) FCS ...
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bioRxiv - Immunology 2023Quote: ... Each sample was injected onto a PepMap C18 trap column (75 μM X 2 cm, 3 μM, 100 Å, Thermo Fisher Scientific, San Jose, CA) at 5 μL/min for 6 min using 0.05% trifluoroacetic acid (TFA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... with each sample being prepared from 5 individuals (3 karyotypes x 2 temperatures x 9 isochromosomal lines = 54 samples) using the MagMAX™-96 Total RNA Isolation Kit (ThermoFisher Scientific, Waltham, MA, USA) on a MagMAX™ Express Magnetic Particle Processor (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). Prior to analyses ...
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bioRxiv - Plant Biology 2024Quote: ... 1cm long root tip sections of four plants were pooled and rolled up in a 1 x 3 cm strip of pre-moistened tissue paper and placed into 2 mL Screw Glass Vials (Thermo Scientific, Cat. No. 6PSV9-1P) filled with 200 μL of water ...
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bioRxiv - Molecular Biology 2020Quote: ... (G4S)6 Linker-VH/CH1 and (G4S)6 Linker-VH/CH1-(G4S)6 Linker-VH/CH1 sequences were synthesized using GeneArt® gene synthesis service (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... Adult cardiac NMCs were isolated from adult C56BL/6 mice (6-8 weeks old) by digesting adult ventricles in buffer containing 1 mg/ml collagenase IV (Gibco 17104-019) and 1.2 mg/ml dispase II (Sigma ...
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bioRxiv - Biochemistry 2020Quote: Beads were first activated with 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (Thermo Fisher Scientific) in the presence of N-hydroxysuccinimide (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The caspase-3 activity was quantified using EnzChek™ Caspase-3 Assay Kit #1 (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) was obtained from Life Technologies (Carlsbad, CA) and N-hydroxysulfosuccinimide (NHSS ...
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bioRxiv - Neuroscience 2022Quote: RNA was 3’ labelled with biotin using Pierce™ RNA 3’ End Biotinylation Kit (ThermoFisher) and purified using Monarch® RNA Cleanup Kit (New England Biolabs).
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bioRxiv - Bioengineering 2024Quote: ... Red blood cells were lysed following 3 × 3 min incubations with ACK Lysing Buffer (Gibco). The remaining white blood cells were washed with PBS twice and plated for surface staining ...
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bioRxiv - Microbiology 2023Quote: ... Head kidney pieces (3 mm × 3 mm) were collected in RNA later (Thermo Fisher Scientific), transferred to 500 μL MagNA Pure LC RNA Isolation Tissue Lysis buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Gdf1/3 and Gdf1/3-like mRNA were synthesized using T7 mMESSAGE mMACHINE kit (Ambion). Unfertilized eggs were injected with mRNAs and fertilized as previously described50 ...