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Citations for Thermo Fisher :
7451 - 7500 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... samples were washed with 2% BSA in PBS for 5 min and mounted using ProLong™ Gold Antifade Mountant with DAPI (ThermoFisher Sci., USA). To preserve islets structural integrity ...
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bioRxiv - Microbiology 2020Quote: ... Samples were concentrated onto the trap column at 5 μl min-1 for 5 min with Buffer A (0.1% formic acid, 2% Acetonitrile) and infused into an LTQ-Orbitrap Elite (Thermo Scientific, San Jose CA) at 300 nl min-1 via the analytical column using a Dionex Ultimate 3000UPLC (Thermo Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Cultures were maintained at 37°C in 5% CO2 for 2 h before the plating medium was replaced with Neurobasal growth medium containing B27 supplements (Life Technologies Inc., USA). The cells were used for experiments after 6 days in culture ...
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bioRxiv - Cancer Biology 2021Quote: ... SK-MEL-2 ATCC® HTB-68™) and cultured in DMEM (Mediatech, Manassas, VA, USA) with 5% FBS (Invitrogen, Carlsbad, CA, USA) and 1% Penicillin-Streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... After pre-concentration and washing of the sample on a Acclaim PepMap 100 C18 column (2 cm × 100 μm i.d., 100 Å pore size, 5 μm particle size, Thermo Scientific 164564-CMD), peptides were separated on a LC EASY-Spray C18 column (50 cm × 75 μm i.d. ...
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bioRxiv - Developmental Biology 2020Quote: Cumulative curve of the thymidine analog 5-Ethynyl-2’-deoxyUridine (EdU) incorporation is performed using Click-iT™ Plus EdU Alexa Fluor™ 647 Imaging Kit (ThermoFisher; C10640). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were pre-concentrated on a C18 trap column (Acclaim PepMap 100; 100 μm × 2 cm, 5 μm, 100 Å; Thermo Fisher Scientific) within seven minutes at a flow rate of 30 μL/min with 0.1 % trifluoric acid and subsequently transferred to a Nano Viper C18 analytical column (Acclaim PepMap RSLC ...
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bioRxiv - Biochemistry 2022Quote: ... of each sample were loaded by 20 μL of 0.1% formic acid in water at a pressure of 200 bar onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å; Thermo Fisher Scientific). The following mobile phases were used ...
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bioRxiv - Microbiology 2022Quote: ... 5 μg of each RNA sample was incubated twice with 2 units of TURBO DNase (TURBO DNA-free Kit, Ambion, Austin, TX, USA) for 30 min at 37 °C ...
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bioRxiv - Immunology 2022Quote: A culture flask containing 6.0 × 106 Vero cells was infected for one hour at 37°C with 0.01 MOI ZIKV in 5 mL of MEM medium with 2% FBS (Serum Fetal bovine; Thermo Fisher Scientific, cat: 12657029). After one hour of infection ...
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bioRxiv - Neuroscience 2020Quote: Proliferation of pericytes was determined by incorporation of the thymine analogue 5-ethynyl-2’-deoxyuridine (EdU) with the Click-iT®Assay Kit (Life Technologies C10340) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... cells (1.5-2×105 cells/mL) were cultured in complete IMDM ((IMDM [Life Technologies], 10% FBS [26140079, Life Technologies], 1% Penicillin-Streptomycin [Life Technologies] ...
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bioRxiv - Cancer Biology 2022Quote: ... Peptides dissolved in 0.1% TFA were pre-concentrated on a C18 trap column (Acclaim PepMap 100; 100 μm × 2 cm, 5 μm, 100 Å; Thermo Fisher Scientific) within 7 min at a flow rate of 30 μl/min with 0.1% TFA ...
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bioRxiv - Cell Biology 2020Quote: ... was centrifuged in 2 tubes to remove cells (300 × g 5 minutes, × 2) and filtered using a double layered 5µm pore nylon Sieve (Fisher Scientific: BioDesign cat 12994257). The supernatant was collected and centrifuged at 2000 × g for 30 minutes and prepared for ISX analysis ...
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bioRxiv - Biochemistry 2021Quote: ... peptides were loaded on an Acclaim PepMap 100 pre-column (100 μm x 2 cm, C18, 5 μm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid at a flow rate of 20 μL/min for 3 min ...
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bioRxiv - Immunology 2020Quote: ... the samples were loaded via an Acclaim PepMap 100 trap column (100 μm × 2 cm, nanoViper, C18, 5 μm, 100å; Thermo-Fisher Scientific, Waltham, MA) onto an Acclaim PepMap RSLC analytical column (75μ m × 50 cm ...
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bioRxiv - Microbiology 2023Quote: ... The ChAd-SARS-CoV-2-BA.5-S genome was rescued following transfection of T-REx™-293 Cell Line (Invitrogen, R710-07). Replication-incompetent ChAd- SARS-CoV-2-BA.5-S ...
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bioRxiv - Cell Biology 2023Quote: ... the cells (or fibers) were washed with PBS and counterstained with 4’6- diamidino-2-phenylindole (DAPI, 5 mg/ml, Molecular Probes, Invitrogen, Carlsbad, USA) in PBS to label nuclei.
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bioRxiv - Neuroscience 2022Quote: ... Attached to the UPLC was an Acclaim PepMap 100 C18 trap (100 µm x 2 cm, 100 Å pore size, 5 µm particle size, Thermo Fisher Scientific) for desalting a purification followed by a nanoEase M/Z peptide BEH130 C18 column (75 µm x 25 cm ...
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bioRxiv - Evolutionary Biology 2022Quote: ... peptides were loaded on an Acclaim PepMap 100 pre-column (100 μm x 2 cm, C18, 5 μm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid at a flow rate of 20 μL/min for 3 min ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were again re-blocked with 5% NGS for 2 hr (second serum blocking) and subsequently stained with rat anti-GFAP (1:500; Invitrogen Cat. #13-0300), and rabbit anti-Megf10 (1 ...
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bioRxiv - Systems Biology 2023Quote: ... The fractionated peptides were reconstituted in 0.5% formic acid (FA) and loaded onto a trap column (Acclaim™ PepMap™ 100, LC C18, 5 μm, 100 μm × 2 cm, nanoViper, Thermo Scientific) at a flow rate of 8 μL/min ...
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bioRxiv - Biochemistry 2023Quote: ... F-actin was incubated for 2 h at room temperature with a 5-fold molar excess of Alexa-488 NHS ester dye (Thermo Fisher Scientific, USA). F-actin was then pelleted by centrifugation at 450,000 × g for 40 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were initially loaded onto a trap column (Acclaim C18 PepMap nano Trap × 2 cm, 100 μm I.D, 5-μ m particle size and 300-Å pore size; Thermo Fisher Scientific) at 15 μl min−1 for 3 min and subsequently switched to the analytical column (Acclaim RSLC C18 PepMap Acclaim RSLC nanocolumn 75 μm × 50 cm ...
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bioRxiv - Immunology 2023Quote: ... The ChAd-SARS-CoV-2-BA.5-S genome was rescued following transfection of T-REx™-293 Cell Line (Invitrogen, R710-07). Replication-incompetent ChAd-SARS-CoV-2-BA.5-S ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were loaded on an Acclaim PepMap 100 pre-column (100 µm x 2 cm, C18, 5 µm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid at a flow rate of 20 µL/min for 3 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The UHPLC system was equipped with a C18 trap column (length 2 cm, particle size 5 µm, pore size 100 Å, inner diameter 100 µm, Acclaim PepMap™ 100, #164199 Thermo Scientific) and a C18 separation column (length 25 cm ...
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bioRxiv - Microbiology 2022Quote: ... glucose-free RPMI 1640 and incubated at 37 °C and 5% CO2 for 2 hours in glucose-free RPMI (Thermo Fisher Scientific, 11879020) supplemented with 10 mM 13C6-glucose (Cambridge Isotope Laboratories ...
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bioRxiv - Biochemistry 2022Quote: ... A 2-fold excess of BODIPY-FL C5-maleimide (FN3-AFF samples) or Fluorescein 5 maleimide (FN3 and cpFN3 samples) (ThermoFisher Scientific, Waltham MA) was immediately added and the reaction was allowed to proceed for 1 h at room temperature or overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: Caco-2 (colorectal adenocarcinoma, ATCC® HTB-37) were grown at 37 °C under 5% CO2 in DMEM low glucose pyruvate (Gibco, 31885-023) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2022Quote: ... 5×103 cells/well were plated 5 days post infection on viewPlate-96black and the next day the cells were treated with 10 µM 5-ethynyl-2’-deoxyuridine EdU (Thermo Fisher, Eugene, OR) for 4 h and fixed in 4% paraformaldehyde in PBS ...
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bioRxiv - Developmental Biology 2023Quote: Cell cycle changes were additionally assessed using the 5-ethynyl-2’-deoxyuridine (EdU) assay using the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (ThermoFisher, C10418). HUH7.5 cells were incubated at 37 °C for 2 hours with 10 µM EdU using the Click-iT EdU Assay Kit (Thermofisher ...
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bioRxiv - Biophysics 2023Quote: ... F-actin was incubated for 2 h at room temperature with a 5-fold molar excess of Alexa-488 NHS ester dye (Thermo Fisher Scientific, USA). F-actin was then pelleted by centrifugation at 450,000 × g for 40 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... EDTA was added to the samples at a final concentration of 5 mM and the suspension was diluted 1:5 in collection buffer (2 % heat-inactivated fetal bovine serum, Life Technologies; 26140079; 25 mM HEPES, Life Technologies; 15630056) in PBS to reduce the collagenase activity ...
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bioRxiv - Biochemistry 2024Quote: ... F-actin was incubated for 2 h at room temperature with a 5-fold molar excess of Alexa-488 NHS ester dye (Thermo Fisher Scientific, USA). F-actin was then pelleted by centrifugation at 450,000 × g for 40 min at room temperature ...
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bioRxiv - Physiology 2024Quote: ... the cell suspension was centrifuged at 300xg for 5 minutes before being resuspended in a pre-warmed neurobasal medium supplemented with 2% B27 supplement (Gibco Invitrogen, CA, USA), 0.25% GlutaMAX (Gibco Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Tissues were placed and minced in a 15-mL conical tube with 5 mL digestion medium (RPMI + 2% FBS) containing 100 mg/mL Dispase (Life Technologies, cat# 10104159001), 100 mg/mL collagenase P (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... F-actin was incubated for 2 h at room temperature with a 5-fold molar excess of Alexa-488 NHS ester dye (Thermo Fisher Scientific, USA). F-actin was then pelleted by centrifugation at 450,000 × g for 40 min at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... were mixed at a 17:2:1 molar ratio (85%, 10%, 5%) and transfected using lipofectamine 2000 (Thermo Fisher Scientific; Cat. No. 11668027) following manufacturer protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were loaded onto an Acclaim PepMap 100 trap column (100 µm × 2 cm, nanoViper, C18, 5 µm, 100 Å, Thermo Fisher Scientific) and separated on an Acclaim PepMap RSLC analytical column (75 µm × 50 cm ...
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bioRxiv - Microbiology 2020Quote: ... The EnzChek Phosphate assay kit and Pierce BCA Protein assay kits were purchased from Life Technologies (USA). E ...
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bioRxiv - Cell Biology 2020Quote: ... and the brains were taken out into pre-cooling Phosphate buffered saline (PBS; Thermo Fisher Scientific, Inc.). Then the cortex was isolated ...
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bioRxiv - Neuroscience 2022Quote: ... Endogenous peroxidase was quenched by incubation of sections in 1.5% hydrogen peroxide/0.005% Triton-X-100 diluted in pH 7.4 sterile phosphate buffered saline (PBS) (Invitrogen) for 20 min ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were washed once with phosphate buffered saline (PBS) solution (Thermo Fisher Scientific, Waltham, MA, USA) followed by media changed to OptiMEM Reduced serum medium (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... and the cells were washed with 10mL Dulbecco’s phosphate-buffered saline (DPBS) (Gibco; without calcium and magnesium) to remove any remaining media ...
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bioRxiv - Bioengineering 2020Quote: ... The thin films were hydrated with 1 mL of Dulbecco’s phosphate-buffered saline (1x PBS) (ThermoFisher Scientific). FM assemblies were achieved through gentle agitation overnight at room temperature using a Stuart SB3 rotator ...
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bioRxiv - Biochemistry 2020Quote: ... Dulbecco’s phosphate buffered saline (DPBS) and Triton X-100 were all obtained from Fisher Scientific (Hampton, NH). Isocitrate ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended in MACS buffer (Dulbecco’s phosphate buffered saline without Ca and Mg [DPBS; Life Technologies] supplemented with 0.5% bovine serum albumin [Sigma-Aldrich] and 2 mM EDTA [Sigma-Aldrich] ...
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bioRxiv - Cell Biology 2021Quote: ... and trans-cardially perfused with phosphate-buffered saline (PBS) followed by 10% neutral buffered formalin (Fisher Scientific). Brains were extracted ...
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bioRxiv - Microbiology 2020Quote: ... and phosphate analyses using an ion chromatograph system (Dionex Integrion HPIC system; Thermo Scientific, Waltham, MA, USA). DIC was analyzed using a total carbon analyzer (TOC-L ...