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Citations for Thermo Fisher :
7401 - 7450 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Infrared exposure levels for INS were selected based on their ability to elicit dynamic calcium responses (>2% increase, dF/F) in NG108 cells loaded with a calcium dye (Fluo-4-AM at 1 μM, ThermoFisher, St. Louis, MO, USA). Radiant exposures for no stimulation ...
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bioRxiv - Biophysics 2020Quote: ... We performed confocal reflectance microscopy on unlabeled fibrin samples (see Fig. 2) and fluorescence imaging (see Fig. 4) on networks polymerized from unlabeled fibrinogen and Alexa488-labeled human fibrinogen (Life Technologies, Bleiswijk, the Netherlands) mixed in a 30:1 molar ratio.
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bioRxiv - Cell Biology 2020Quote: ... Nucleoporin-specific antibody was used for primary staining (over-night incubation at 4°C) and and AlexaFluor-conjugated secondary antibodies (Invitrogen; 2 hours at RT). Microscopy was performed as previously described 16 ...
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bioRxiv - Microbiology 2021Quote: ... Healthy cell culture of the primmorphs (2–4 mm in diameter) of green color were transferred to the 24-well plates (Nalge Nunc International, Rochester, NY, USA), 1 pieces per well ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysates were incubated for 2 h at 4°C with IgG-coupled Dynabeads (Dynabeads M-270 Epoxy; Invitrogen, Thermo Fisher Scientific, Basel, Switzerland). The beads were washes 5 times with lysis buffer and proteins were eluted in TEV buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... A baculovirus stock carrying the gene for DNMT1 was used to infect 4–12 l of Sf9 cells at a density of 2 million cells/ml in Sf-900™ III SFM (Invitrogen cat # 12658–027). Multiple 1 l cell cultures in 4 l flasks (VWR cat # 32645–044 ...
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bioRxiv - Biochemistry 2022Quote: ... the cleared lysates were incubated for 2 h at 4°C with prewashed anti-c-myc MagBeads (Pierce Thermo Fisher Scientific, product number 88843). After five washes with lysis buffer ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were then incubated for 2 h at 4℃ with Alexa fluor 488-conjugated secondary antibody (1:200, Invitrogen, #A21206, Carlsbad, CA, USA) or DAPI (1:30000 ...
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bioRxiv - Neuroscience 2022Quote: ... acutely dissociated DRG neurons from 4 mice were first incubated in at 37 °C for 30 minutes in 4 mm Fura-2-acetoxymethyl ester and 0.2% Pluronic® F127 (Thermo Fisher Scientific, Waltham, MA), washed three times ...
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bioRxiv - Cell Biology 2022Quote: ... 1:200 O/N at 4 °C) and Alexa Fluor 594-conjugated anti-mouse IgG antibody (#A11005, Invitrogen, 1:2000 for 2 hours RT). Nuclei staining was done in 0.5 μg/mL Hoechst 33324 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Tris-Glycine 10-20% or Bis-Tris 4-12% SDS-PAGE gels were run at 4℃ and proteins were transferred onto nitrocellulose membranes using 15 Volts for 7 minutes via the iBlot-2 system (Thermo Fisher Scientific, Waltham, MA). The membrane was then blocked for 1 hour in LICOR Odyssey blocking buffer and incubated at 4℃ with the appropriate antibody overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Next day the cells were washed with fresh media and incubated at 37°C for 45 min in culture media containing 4 μM Fura-2/acetoxymethyl ester (Molecular Probes, Eugene, OR, USA). Then the cells were washed and kept in HEPES-buffered saline solution (140 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... CKY2038 was grown to mid-log (1-2×107 cells/mL) in SC liquid media and added to the ConA treated perfusion chamber gasket (ThermoFisher, 4 chamber: 19 × 6mm) for treatment at indicated conditions (SC+1% DMSO ...
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bioRxiv - Cancer Biology 2020Quote: ... and ITS (5 μg/mL insulin, 5 μg/mL transferrin and 5 ng/ml sodium selenite, Invitrogen). SK-N-BE (2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were subject to reducing SDS-PAGE and immunoblotted using antibodies recognizing recombinant protein (anti-NRG1 NRG1abcam ab180808 1:5000 dilution in 5% milk) or Lamin A (Invitrogen MA1-06101, 1:1000 in 3% BSA).
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Molecular architecture determines brain delivery of a transferrin-receptor targeted lysosomal enzymebioRxiv - Neuroscience 2021Quote: ... Sections were then rinsed in 1xPBS/0.05% Tween for 3 rounds of 5 minutes and incubated in DAPI (Invitrogen Molecular Probes D1306: 1:10,000 from 5mg/mL stock) for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... and 4% FBS (Gibco). Plates were incubated 2 days at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... 4 μl pyrophosphatase (Thermofisher), 2 μl Phi29 Polymerase (Thermofisher) ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa 594 (4%, Invitrogen) and neurobiotin (0.05% ...
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bioRxiv - Neuroscience 2022Quote: ... 4 mM Glutamax (Invitrogen), penicillin (100 U/mL) ...
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bioRxiv - Bioengineering 2022Quote: ... Claudin-4 (Invitrogen; 329494), TRPV6 (Proteintech;13411-1-AP) ...
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bioRxiv - Microbiology 2021Quote: ... 4 nM NTPs (ThermoFisher) and 50U Superase (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... at 4°C (Nunc MaxiSorp flat bottom ...
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bioRxiv - Cancer Biology 2021Quote: ... 4% in PBS (Affymetrix) was used to fix cells ...
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bioRxiv - Cell Biology 2021Quote: ... 4 mM glutamine (Gibco), 100 units/ml of penicillin ...
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bioRxiv - Microbiology 2022Quote: ... 4-chlorobenzenesulfonate (DiD, Invitrogen), as previously described [10] ...
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bioRxiv - Neuroscience 2022Quote: ... 4 mM Glutamax (Invitrogen), penicillin (100 U/mL) ...
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bioRxiv - Bioengineering 2024Quote: ... and 4 % formaldehyde (Invitrogen) were purchased form Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... 4% PFA (Thermo Fisher) was added into each spleen section and incubated at RT for 20 mins to fix the tissue ...
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bioRxiv - Biophysics 2023Quote: ... 4 mM glutamine (Gibco), 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Bioengineering 2022Quote: ... 4 mM Glucose (Gibco) and 50 ng/mL IGF-1 (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... 4-12% (Thermo Fisher) with Bolt MES SDS Running Buffer (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... 4% EGF (ThermoFisher Scientific), and 50 ng/mL KSR (Gibco)] ...
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bioRxiv - Synthetic Biology 2023Quote: ... 4-20% (ThermoFisher; XP04205BOX) under reducing and denaturing conditions ...
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bioRxiv - Synthetic Biology 2023Quote: ... 4-20% (ThermoFisher; XP04205BOX) under reducing and denaturing conditions ...
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bioRxiv - Synthetic Biology 2023Quote: ... 4-20% (ThermoFisher; XP04205BOX) under reducing and denaturing conditions ...
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bioRxiv - Cell Biology 2024Quote: ... 4) HBSS (Gibco, 14025092) for 24 hr ...
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bioRxiv - Neuroscience 2024Quote: ... Fluo-4 (Thermo Fisher) was used to study Ca2+ mobilisation ...
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bioRxiv - Genomics 2024Quote: ... 4–12% (Invitrogen, #NP0322BOX) for 1 hour at 120 V in 1x NuPAGE™ MES SDS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... fixed and blocked HeLa CCL-2 cells were incubated with 5 μg/mL Cy5 labeled rabbit anti-Ki67 primary antibodies (Thermo Fisher Scientific; RB1510P1ABX) in the 1X blocking buffer for 45 min at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... FseI restriction sites introduction by Phusion Green High-Fidelity DNA Polymerase PCR, using manufacturers protocol (30cycles, 2-step protocol, no GC additive, 5% DMSO) (Thermo Fisher Scientific, F534S), and JM120F/R ...
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bioRxiv - Developmental Biology 2021Quote: A dam at E16.5 in gestation was given an intraperitoneal (IP) injection with EdU (5-ethynyl-2’-deoxyuridine) from the Click-iTTM EdU Alexa FluorTM 647 Imaging Kit (Invitrogen, Carlsbad, CA, USA) at a dosage of 0.25mg /10g body weight ...
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bioRxiv - Immunology 2022Quote: ... 20 minutes in 30 mL mucus dissociation solution (5 mM dithiothretol [DTT; Invitrogen 15508] and 2% heat-inactivated fetal calf serum [FCS; Gibco A38401] in HBSS); 25 minutes in 30 mL epithelial removal solution (5 mM EDTA and 2% FCS in HBSS) ...
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bioRxiv - Bioengineering 2020Quote: ... chips were washed with 100 ul PBS per channel and stained with the following secondary antibodies diluted 1: 100 in 5% BSA in PBS for 2 hours at room temperature: goat anti-rat Alexa Fluor 488 (Thermo Fisher Scientific A11006), donkey anti-rabbit Alexa Fluor 647 (Thermo Fisher Scientific A31573) ...
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bioRxiv - Cell Biology 2021Quote: ... samples were washed with 2% BSA in PBS for 5 min and mounted using ProLong™ Gold Antifade Mountant with DAPI (ThermoFisher Sci., USA). To preserve islets structural integrity ...
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bioRxiv - Microbiology 2020Quote: ... Samples were concentrated onto the trap column at 5 μl min-1 for 5 min with Buffer A (0.1% formic acid, 2% Acetonitrile) and infused into an LTQ-Orbitrap Elite (Thermo Scientific, San Jose CA) at 300 nl min-1 via the analytical column using a Dionex Ultimate 3000UPLC (Thermo Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Cultures were maintained at 37°C in 5% CO2 for 2 h before the plating medium was replaced with Neurobasal growth medium containing B27 supplements (Life Technologies Inc., USA). The cells were used for experiments after 6 days in culture ...
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bioRxiv - Cancer Biology 2021Quote: ... SK-MEL-2 ATCC® HTB-68™) and cultured in DMEM (Mediatech, Manassas, VA, USA) with 5% FBS (Invitrogen, Carlsbad, CA, USA) and 1% Penicillin-Streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... After pre-concentration and washing of the sample on a Acclaim PepMap 100 C18 column (2 cm × 100 μm i.d., 100 Å pore size, 5 μm particle size, Thermo Scientific 164564-CMD), peptides were separated on a LC EASY-Spray C18 column (50 cm × 75 μm i.d. ...
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bioRxiv - Developmental Biology 2020Quote: Cumulative curve of the thymidine analog 5-Ethynyl-2’-deoxyUridine (EdU) incorporation is performed using Click-iT™ Plus EdU Alexa Fluor™ 647 Imaging Kit (ThermoFisher; C10640). Briefly ...