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Citations for Thermo Fisher :
7101 - 7150 of 10000+ citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Cells were incubated in presence of either 1 μM or 5 μM MitoSoxTM (Invitrogen; Cat. #M36008) for 30 minutes in medium containing Hoechst (1/2,000 dilution) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were passaged every 4–5 d with UltraPure 0.5 mM EDTA (Thermo Fisher Scientific, 15575020). For the generation of 3D neural organoids ...
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bioRxiv - Physiology 2023Quote: ... RT-qPCR was performed in a QuantStudioTM 5 Real-Time PCR System (Applied Biosystems, Massachusetts, USA). Genes were amplified employing the AceQ ® qPCR SYBR Green Master Mix (NeoBiotech ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoblots were then incubated in secondary goat anti-rabbit (1:1000 TBST/5% milk; Invitrogen, 32460) 1h at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... The 5’ arm was amplified to TOPO clone it directly into the pENTRD-TOPO vector (Invitrogen), then recombined into the modified pJHY-TMp1 using Gateway LR Clonase II (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were cultured at 37°C and 5% CO2 in the media: RPMI 1640 (Life Technologies), 10% HIFBS (Gibco) ...
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bioRxiv - Plant Biology 2023Quote: ... the peptides were sampled on a precolumn (300 μm x 5 mm PepMap C18, Thermo Scientific) and separated in a 75 μm x 250 mm C18 column (PepMap C18 ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-time qPCR was performed using Applied biosystem QuantStudio 5 real time PCR System (Thermo Scientific). PrimeTime qPCR probe assays (Supplementary Tables 4 and 5 ...
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bioRxiv - Molecular Biology 2023Quote: ... after the addition of cel-miR-39 spike-in for normalization (5 pmol/µl) (ThermoFisher Scientific). MicroRNAs and U6 small nuclear RNA (supplementary table 2 ...
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bioRxiv - Neuroscience 2023Quote: ... Protein (15 μg) was incubated with 5 μl NuPAGE® Loading Buffer (4x, Life Technologies, NP0008), 2 μl 1 M DTT and made up to 20 μl with deionised water at 100°C for 5 mins ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated with 5 µg/ml OKT4 anti-CD4 antibody (Thermo Fisher Scientific) or a mixture of the 2G12 and PGT121 antibodies against gp120 for 1 hour at 4°C ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... HEK293 cells were maintained at 37°C with 5% CO2 in DMEM media (ThermoFisher Cat #11965118) supplemented with 10% serum (Serum Plus II ...
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bioRxiv - Molecular Biology 2023Quote: ... In vitro transcription was performed using the MEGAscript™ T7 Transcription Kit (Invitrogen, AMB 1334-5) by incubating the reaction at 37 ⁰C for 14 hrs ...
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bioRxiv - Molecular Biology 2023Quote: ... pellets were centrifuged and air dried for 5 min before addition of RNase-free H2O (Ambion). RNA was quantified using the Qubit BR kit (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... The mixture of extracted blood was mixed with 5 μL of 10 mM N-ethylmaleimide (ThermoFisher) in DPBS following previous methods for arresting cellular metabolic activity.39
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bioRxiv - Bioengineering 2023Quote: ... then mixed with 1.2 mL of MgCl2 and 5 uL of SYBR™-Safe (Invitrogen, S33102), and casted into the gel tray ...
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bioRxiv - Biophysics 2023Quote: ... supplemented with 5% (v/v) fetal bovine serum (FBS) and 1% (v/v) antibiotic-antimycotic (Gibco). HEK293S GnTI- cells (ATCC CRL-3022 ...
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bioRxiv - Biochemistry 2023Quote: ... Each well was loaded with 100 µL of a 5 µM Fluo-4 AM (Molecular Probes) solution in imaging buffer (20 mM HEPES ...
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bioRxiv - Cancer Biology 2023Quote: ... and suspended in ice cold 50 µl A-Ri-Buffer (5% FBS, 1% GlutaMAX-I, Invitrogen, 35050-079 ...
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bioRxiv - Cancer Biology 2023Quote: ... RBC lysis was performed for 5 minutes using ACK lysis buffer (Cat. A1049201, Thermo Fisher Scientific) at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... we incubated 5-7 μg of BACMID DNA with 4 μL Fugene (Thermo Fisher, Cat# 10362100) in 250 μL of Opti-MEM serum free media for 30 minutes at 23ºC ...
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bioRxiv - Cell Biology 2023Quote: ... and boiled for 5 minutes before fractionating on a 4–12% NuPAGE Bis-Tris gel (Invitrogen) and transferred to nitrocellulose membrane ...
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bioRxiv - Cell Biology 2023Quote: ... COS-7 cells were grown at 37 °C under 5% of CO2 in DMEM Glutamax (Gibco) with 10% bovine fetal serum ...
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bioRxiv - Immunology 2023Quote: ... pre-coated (5 μg cm−2) 4-well chamber slide (Thermo Fisher Scientific, cat. no. 154526). A fluorescence image was captured using an LSM 880 confocal microscope (Carl Zeiss AG ...
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bioRxiv - Microbiology 2023Quote: ... at 3500 rpm for 5 minutes (Sorvall Legend X1R M20 rotor, Thermo Scientific, Waltham, MA, USA). The sterile bacterial supernatants were stored at −80°C until subsequent use.
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bioRxiv - Neuroscience 2023Quote: RT-qPCR was performed in a QuantStudioTM 5 Real-Time PCR System (Applied Biosystems, Massachusetts, USA). Genes were amplified employing the AceQ® qPCR SYBR Green Master Mix (NeoBiotech ...
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bioRxiv - Synthetic Biology 2023Quote: ... a qPCR approach was set in a QuantStudio™ 5 Real-Time PCR system (ThermoFisher Scientific). Expression levels of the aadA gene ...
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bioRxiv - Microbiology 2023Quote: ... 50 µg/ml gentamycin sulfate) supplemented with 4 to 5 µg/ml ConA (Thermo Scientific AAJ61221MC). To determine the effects of type I interferons ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were selected and maintained in the presence of 5 μg/mL blasticidin (Thermo Fisher, MA). Monoclonal cells were isolated from a polyclonal pool of transduced stable cells using the limiting dilution assay ...
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bioRxiv - Developmental Biology 2023Quote: ... GV oocytes were incubated in M2 media containing 5 μM MitoSOX Red (ThermoFisher, M36008, Waltham, USA,) in humidified atmosphere for 10 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: Isolated monocytes were resuspended in complete RPMI media with 2mM L-glutamine and 5% FCS (Gibco) and seeded in a 24-well cell culture plate at 5 x 105 cells/well ...
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bioRxiv - Systems Biology 2023Quote: ... This reaction involved the following components per sample: 44 μL of 5× RT Buffer (Thermo Scientific), 44 μL of 20% Ficoll PM 400 (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... at 37°C under 5% CO2 in Dulbecco’s Modified Eagle’s Medium (Gibco, Cat. no: 12800-017) supplemented with 10% FBS (Gibco ...
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bioRxiv - Plant Biology 2023Quote: ... 25 µl of extracted protein samples were mixed with NativePAGE 5% G-250 sample additive (Invitrogen). 5 µl of each sample was loaded to NativePAGE 4 to 16% bis-tris gels (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... added to 2 μL of TruCut Cas9 Protein v2 (5 ng/μL, Thermo Fisher Scientific A36498), and incubated for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the frozen sections were blocked with 5% normal goat serum (NGS) (Thermo Fisher Scientific, Cat# 31872) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 15-20 pooled day 30 neurospheres and 5-7 pooled day 60 organoids using TRIzol (Invitrogen; Thermo Fisher Scientific Inc. ...
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bioRxiv - Cell Biology 2023Quote: ... centrifuged at 1,000 rpm for 5 min and resuspended in DMEM:F-12 (1:1) (Gibco, C11330500BT) media supplemented with 10% FBS and 1% penicillin- streptomycin ...
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bioRxiv - Microbiology 2023Quote: ... Reactions were run on a QuantStudio 5 Real-Time PCR Instrument (A28133, Applied Biosystems, Waltham, MA). The results were analyzed with the Qiagen analysis spreadsheet provided with the kit.
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bioRxiv - Evolutionary Biology 2023Quote: ... per liter] and 20% glycerol on an SM/5 agar media [2 g glucose (Fisher Scientific), 2 g yeast extract (Oxoid) ...
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bioRxiv - Biophysics 2023Quote: ... 5 µL sample or standard was added to a clear flat bottom 96-well plate (Nunc). The glucose reagent was pre-heated to 37 °C and the 95 µL reagent was added to start the reaction ...
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bioRxiv - Neuroscience 2023Quote: ... FFPE slides of 5 micrometres were prepared with a microtome (Thermo Fisher Scientific, Microm HM 355S).
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bioRxiv - Genomics 2023Quote: Wildtype MCF10A cells (ATCC #CRL-10317) were cultured at 5% CO2 in DMEM/F12 (ThermoFisher, #11320033) supplemented with 5% Horse Serum (ThermoFisher ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... A375 cells were maintained at 37°C with 5% CO2 in DMEM media (ThermoFisher Cat #11965118) supplemented with 10% serum (Serum Plus II ...
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bioRxiv - Microbiology 2023Quote: NaOCl disinfectant was purchased as a solution containing 5% or 50,000 ppm active chlorine (Acros Organics) and stored at 4°C until use ...
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bioRxiv - Microbiology 2023Quote: ... Cells transduced with Lenti-Spike were treated with 5 µg/ml Hoechst solution (Molecular Probes®) before being analyzed under a microscope ...
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bioRxiv - Bioengineering 2023Quote: The primer and template labeled at the 5’-terminus by Cy5 dye were ordered from Invitrogen. The primer-template (P/T-2Cy5 ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary AlexaFluor-conjugated antibodies (Life Technologies; 1:500 in 5% FBS, 0.1% Triton-X in PBS) with Hoechst 33342 dye were incubated for 1 h at room temperature in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... and 5 ml of 0.08% trypsin containing 0.35 mM EDTA (25200-072, Life Technologies, Somerset, NJ) in Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Neuroscience 2022Quote: ... the media was changed to 1:1 KSR: N2B media with puromycin (5 μg/ml, Gibco), where N2B was composed of DMEM/F12 ...