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Citations for Thermo Fisher :
6951 - 7000 of 10000+ citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Pellets were diluted to a concentration of 5 × 104 cells/μL with ACK lysis buffer (Gibco). Samples were mixed by pipette and incubated at room temperature for 5 min ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from 5 × 106–10 × 106 hybridoma cells using TRIzol reagent (Invitrogen, Gaithersburg, MD). Heavy (H)- and light (L)-chain cDNAs containing the gene encoding the antibody-binding (Fab ...
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bioRxiv - Neuroscience 2022Quote: ... and 5 ml of 0.08% trypsin containing 0.35 mM EDTA (25200-072, Life Technologies, Somerset, NJ) in Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Neuroscience 2022Quote: ... the media was changed to 1:1 KSR: N2B media with puromycin (5 μg/ml, Gibco), where N2B was composed of DMEM/F12 ...
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bioRxiv - Immunology 2022Quote: ... absolute cell numbers were quantified using 5 µL of 123count eBeads Counting Beads (Thermo Fisher Scientific) added to each sample.
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bioRxiv - Cell Biology 2022Quote: ... were grown at 37°C with 5% (vol/vol) CO2 in Dulbecco’s Modified Eagle Medium (Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Neuroscience 2022Quote: ... washed 3 × 5 min with TBS and embedded in ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific). Images were taken with a Nikon Eclipse90i microscope and acquisition was performed with the NIS Elements software (Version 5.01) ...
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bioRxiv - Bioengineering 2022Quote: ... and subsequent incubation at 37°C with 5% CO2 in a cell incubator (Thermo Fisher Scientific) for another 45 min ...
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bioRxiv - Bioengineering 2022Quote: ... we embedded 5 μm fluorescent microspheres and a separate sample of 10 μm microspheres (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were maintained at 37°C with 5% CO2 in high glucose DMEM (Gibco, 11965-092) supplemented 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... qPCR experiments were performed using QuantStudio 5 Real-time PCR system (Thermo Fisher Scientific, MA, USA) and SYBR Green Master Mix (Roche) ...
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bioRxiv - Cancer Biology 2022Quote: ... were obtained from American Type Culture Collection (ATCC) and cultured at 37°C and 5% CO2 in either RPMI-1640 (Gibco 11835-030) or Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Biophysics 2022Quote: ... The RNA digestion was performed by addition of 5 U of RNase T1 (Thermo Fisher Scientific) and 5 μg of RNase A (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were sectioned at 5 μm using a rotary microtome (Thermo Fisher Microm HM310 Rotary Microtome) and affixed to clean slides.
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bioRxiv - Cancer Biology 2022Quote: ... TaqMan Assay reaction mixtures contained: 5 μL of TaqMan® Fast Advance (Applied Biosystems, Warrington, UK), 2.5 μL nuclease-free H2O ...
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bioRxiv - Biochemistry 2022Quote: ... Dialyzed CsgF protein was then passed through a 5 mL Zeba spin desalting column (Thermo Fisher) that was equilibrated with 25 mM Tris pH 9 buffer ...
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bioRxiv - Bioengineering 2022Quote: ... Image-iTTM Red Hypoxia Reagent (5 μM) and Cell Tracker DeepRed (0.1 μM) (all from Invitrogen). Cell samples were split into five equal volumes and subsequently encapsulated in 5.6 nL droplets in a continuous phase of fluorinated oil Novec HFE-7500 (3M ...
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bioRxiv - Cell Biology 2022Quote: ... were cultured at 37°C and 5% CO2 in DMEM/Glutamax (#31966; Gibco, Invitrogen Life Technologies) supplemented with 10% FCS ...
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bioRxiv - Cell Biology 2022Quote: ... were cultured at 37°C and 5% CO2 in DMEM/Glutamax (#31966; Gibco, Invitrogen Life Technologies) supplemented with 10% FCS ...
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bioRxiv - Cell Biology 2022Quote: ... were cultured at 37°C and 5% CO2 in DMEM/Glutamax (#31966; Gibco, Invitrogen Life Technologies) supplemented with 10% FCS ...
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bioRxiv - Cell Biology 2022Quote: 200,000 cardiomyocytes were incubated in Amplex Red (5 μM; 2 min) for cellular ROS (A22177, ThermoFisher), or MitoSOX Red (5 μM ...
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bioRxiv - Cell Biology 2022Quote: HEK293 cells were cultured at 37°C and 5% CO2 in DMEM (Thermo Fisher Scientific; 11965092) supplemented with 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: We maintained all cells at 37°C and 5% CO2 in a Heracell incubator (Thermo Fisher). We cultured HEK293 cells in minimum essential medium (MEM ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-EU visualization used hapten-containing azide and Click-iT Cell Reaction Buffer Kit (Thermo Fisher). Samples were washed twice before imaging ...
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bioRxiv - Cell Biology 2022Quote: ... and centrifuged at 1000 rpm for 5 min at 4°C (accuSpin Micro 17R; Fisher Scientific). The proteins were extracted in 50 mM Tris buffer (pH 7.8 ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 nM (final concentration) shRNA or 3 ∼ 5 µg plasmid in 50 µL Opti-MEM (Gibco) was mixed with 5 µL transfection reagent (Lipofectamine ...
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bioRxiv - Immunology 2022Quote: ... IVT was then performed using MEGAscript® T7 Transcription Kit (Thermo Fisher Scientific, AMB-1334-5) according to the manufacturer’s instructions with modifications as using the CleanCap® Reagent AG (TriLink ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cells were grown in a humidified 37°C (5% CO2) atmosphere in MEM α (ThermoFisher, #32571101) supplemented with 5% HyClone fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: The samples were counterstained using 5 ng ml-1 DAPI dissolved in SlowFade Gold (Invitrogen, USA) and covered with a #1.5 high precision coverslip (Marienfeld ...
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bioRxiv - Microbiology 2022Quote: ... cells were pelleted and resuspended in 50 ul of 5 µg/ml FM4-64 (Molecular Probes). Three microliters of suspension were placed on a microscope slide and immobilized with a poly-L-lysine-treated coverslip.
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bioRxiv - Molecular Biology 2022Quote: ... the peptides were sampled on a precolumn (300 μm x 5 mm PepMap C18, Thermo Scientific) and separated in a 75 μm x 250 mm C18 column (Reprosil-Pur 120 C18-AQ ...
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bioRxiv - Microbiology 2022Quote: ... Protein was then crosslinked to beads with 5 mg/ml DTSSP (Thermo Fisher Scientific, Rockville, MD) in binding buffer for 45 min at RT ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was assayed by qPCR on the QuantStudio 5 – Real-Time PCR System (Thermo Fisher) using TaqMan Universal PCR Mastermix (Thermo Fisher) ...
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bioRxiv - Immunology 2022Quote: ... 2-5×106 cells were incubated in 15 μl Indo-1 solution in 1ml IMDM (Gibco) + 1%FBS (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... RNA was isolated by adding 5 volumes of Trizol LS Reagent (Life Technologies, Carlsbad, CA, USA) followed by a 5 min incubation at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Blots were washed 3x 5 min in TBST and 2mL each SuperSignal Substrate (Thermo Fisher Scientific) reagent was added to blot and incubated for 5 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... PV-3 SC8 VLPs or infectious PV-1 were incubated with 5 µM SYTO9 (Thermo Fisher) and SYPRO red (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... Data analysis was performed with the Quantstudio 3&5 Design and analysis software (Applied Biosystems, v1.5.1). Cq values were obtained via automated threshold determination ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 ug of RNA was converted to cDNA using Maxima H Minus Reverse Transcriptase (ThermoFisher, EP0751) according to manufacturer instructions and the resulting cDNA was used for capsid sequence recovery ...
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bioRxiv - Plant Biology 2022Quote: ... The 10 μl reaction mixture contained 5 μl 2X SYBR Green/ROX master mix (Applied Biosystems), 2 μl of 1:20 diluted cDNA ...
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bioRxiv - Systems Biology 2022Quote: ... Experimental starvation medium is DMEM/F12 medium supplemented with 5% (by volume) horse serum (Gibco #16050122), 0.5 mg/mL hydrocortisone (Sigma #H-0888) ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were washed with D-PBS and blocked with 5% horse serum (Thermo Fisher, #26050070), 1% BSA in D-PBS with 0.3% Triton X-100 for 1 h (RT) ...
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bioRxiv - Neuroscience 2022Quote: ... QPCR was performed with Applied Biosystems QuantStudio 5 Real-Time PCR System (ThermoFisher Scientific, MA, USA). Oligonucleotide primers are listed in Table S1 and primers that amplify Actb and Gapdh were used as reference genes ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary GR antibody (G-5, Santacruz sc-393232, 1:100) or MAP2 antibody (Invitrogen, 1:2000) incubations lasted for 1 h at RT followed by 3x washes and 1 h incubation in secondary antibody at RT (Cy3 Goat anti-mouse against GR ...
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bioRxiv - Developmental Biology 2022Quote: ... 5 µg of total RNA per sample was treated with 2U of TURBO DNase (ThermoFisher Scientific) for 1 h.
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bioRxiv - Microbiology 2023Quote: ... Presumptive macrococci colonies were streaked onto blood agar supplemented with 5% sheep blood (Oxoid, ThermoFisher, Johannesburg), incubated for 24 hours at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Gc were pelleted and resuspended in 5 μg/mL goat anti-mouse AF488 (Invitrogen, Carlsbad, CA) diluted in RPMI + 10% FBS for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... PBS washes (3 × 5 min) and incubation with primary antibody (mouse anti-GFP, A11120, Life technologies; 1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... washed for 5 min with PBS and mounted with Permafluor (Thermo Fisher Cat# TA-006-FM). Primary mixed glial cultures were fixed for immunofluorescence staining at 5 ...
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bioRxiv - Neuroscience 2022Quote: ... washed for 5 min with PBS and mounted with Permafluor (Thermo Fisher Cat# TA-006-FM). The following primary antibodies were used ...