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Citations for Thermo Fisher :
7001 - 7050 of 10000+ citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Each 10 µl reaction contained 5 µl SYBR GreenER™ qPCR SuperMix Universal (Thermo Fisher Scientific), forward and reverse primer at a concentration of 200 nM each ...
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bioRxiv - Neuroscience 2023Quote: ... and then blocked for two hours in 5% BSA/PBS (BP1600, Fisher Scientific, Fair Lawn, NJ) at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and stained with 5 μg mL−1 Alexa Fluor 568 donkey anti-rabbit IgG (Invitrogen, A10042) for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Sample was loaded onto the trapping column (Thermo Scientific, PepMap100, C18, 300 um x 5 mm), using partial loop injection for 3 min at a flow rate of 15 µL/min with 0.1% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... and boiled for 5 min before separation on a 4-12% NuPAGE Bis-Tris gel (Invitrogen) for immunoblotting.
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bioRxiv - Microbiology 2023Quote: BSRT7/5 cells at 90% confluence in 96-well dishes were transfected using Lipofectamine 2000 (Invitrogen) with a plasmid mixture containing 62.5 ng of pGaussia/Firefly minigenome ...
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bioRxiv - Synthetic Biology 2023Quote: ... Samples (using 25 μL each) including 5 μL PageRuler Plus (Thermo Fisher Scientific, Waltham, MA, USA) or Precision Plus Dual Xtra (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... Further add 0.5 µl of NativePAGE 5% G250 sample additive (Invitrogen, Thermo Fisher Scientific, Carlsbad, CA) to the 19.5 µL of the previous mixture and mix it well ...
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bioRxiv - Systems Biology 2023Quote: ... membranes were incubated for 5 min in SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Fisher, 34579) according to manufacturer’s instruction just prior to detection on a Bio-Rad ChemiDoc ...
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bioRxiv - Systems Biology 2023Quote: ... and transferred to a conical tube containing 5 mL of 1X CD CHO Medium (Gibco, 10743011) bubbled with carbogen ...
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bioRxiv - Developmental Biology 2023Quote: ... washed with PBS three times (5 min each) and mounted with Shandon Immuno-Mount (Thermo Scientific). The antibodies and dilutions employed for cell immunofluorescence were the following ...
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bioRxiv - Physiology 2023Quote: ... R: 5’ TGCCTTCGTGCCGTACTG 3’) and confirmed by DNA sequencing following sub-cloning into pCRTM-Blunt (Invitrogen). Further genotypes were determined by real-time PCR with specific probes designed for each gene (Transnetyx ...
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bioRxiv - Genomics 2023Quote: ... This mixture was incubated at 65°C for 5 min in an ABI2700 thermocycler (Applied Biosystems) then ramped down to 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were cultured in DME/F12 media supplemented with 5% horse serum (Thermo Fisher Scientific, 16050122), 20 ng/mL human Epidermal Growth Factor (hEGF ...
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bioRxiv - Genomics 2023Quote: ... This mixture was incubated at 65°C for 5 min in an ABI2700 thermocycler (Applied Biosystems) then ramped down to 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR (qPCR) was performed on the QuantStudio™ 5 Real-Time PCR System (Thermo Scientific) using PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... the peptides were sampled on a precolumn (300 μm x 5 mm PepMap C18, Thermo Scientific) and separated in a 75 μm x 250 mm C18 column (Aurora Generation 2 ...
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bioRxiv - Microbiology 2023Quote: ... Ten μL reaction volumes containing 5 μl SYBR Select Master Mix (Life Technologies, Grand Island, NY), 400 nM of primer attR-F and attL-R (Table 2) ...
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bioRxiv - Neuroscience 2023Quote: ... followed by an approximately 5 minute incubation with EDTA (0.5 mM, pH 8.0, Thermo Fisher Scientific) for maintenance or TrypLE (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... larval fat bodies at 5 and 12 days AEL were dissected in Schneider’s S2 medium (GIBCO). After incubation in 30 μM DHE (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were split every 5-7 days by incubation with 0.5 μM EDTA (Thermo Fisher Scientific) for 4 min at room temperature and clumps were dissociated into small clumps by pipetting ...
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bioRxiv - Pathology 2023Quote: ... Mice were perfused via the left ventricle with 5 mL of ice cold HBSS (14025092, Gibco) after which perfused kidneys were collected and placed on ice in HBSS ...
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bioRxiv - Microbiology 2023Quote: ... based on a similar strategy previously reported [5] but using the Phi29 DNA Polymerase (Thermo Scientific) and newly designed primers (Table S1) ...
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bioRxiv - Biochemistry 2023Quote: ... and were maintained in DMEM supplemented with 5% FBS and 1% GlutaMax (Life Technologies, Carlsbad, CA) in a 37°C CO2 incubator ...
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bioRxiv - Cancer Biology 2023Quote: ... the media was replaced with complete DMEM/F12 containing 5 μg/mL puromycin (Gibco, Waltham, MA). Once at 90% confluence in the 6-well plate ...
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bioRxiv - Plant Biology 2023Quote: ... RNA samples (5 μg) were treated with DNase I using the Turbo DNA-free Kit (Ambion). RNA integrity was analyzed in 1 % agarose gel ...
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bioRxiv - Cell Biology 2024Quote: ... and reversed transfected at a concentration of 5 nmol/L using Lipofectamine RNAiMAX transfection reagent (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... Cells were collected into fluorescence-activated sorting tubes and co-stained with 5 µM 7AAD (Invitrogen) and 1X BD Pharmingen™ PE Annexin V (BD Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... washed for 5 min with PBS and mounted with Permafluor (Thermo Fisher Cat# TA-006-FM). Primary mixed glial cultures were fixed for immunofluorescence staining at 5 ...
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bioRxiv - Neuroscience 2022Quote: ... washed for 5 min with PBS and mounted with Permafluor (Thermo Fisher Cat# TA-006-FM). The following primary antibodies were used ...
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bioRxiv - Neuroscience 2022Quote: ... The membranes were blocked using either 5% milk or Superblock (PBS) Blocking Buffer (ThermoFisher, Cat#37515). Primary antibody was applied overnight at 4°C for PDE11A (Fabgennix PD11-101 at 1:500) ...
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bioRxiv - Neuroscience 2022Quote: ... followed 200 µl 0.2% TFA/5%ACN (ThermoFisher Scientific; Cat#: 28904; Thermo Fisher Scientific; Cat#: A955)in a centrifuge at 3000 rpm ...
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bioRxiv - Neuroscience 2022Quote: ... was dissolved in 5% DMSO and then diluted with saline solution (DPBS, 14190-144, Life Technologies) to reach 1 mg/mL CNO and 0.25% DMSO ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented either with 5 gm/L AlbuMax I (for 3D7, R539T and RKL-9; Gibco, USA) or 10% heat inactivated human A+ serum (for NF54 ...
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bioRxiv - Molecular Biology 2022Quote: ... reagent in technical duplicates using the QuantStudio™ 5 Real-Time PCR cycler (Thermo Fisher Scientific) and following conditions ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR was carried out on a QuantStudio 3 or QuantStudio 5 Real-Time PCR System (ThermoFisher) with a passive reference of ROX using the following cycling conditions ...
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bioRxiv - Immunology 2022Quote: ... 4×104 sorted peritoneal and thymic macrophages were stained with 5 µM eFluor 450 (Thermo Fisher) in PBS for 10 min at 37℃ ...
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bioRxiv - Neuroscience 2022Quote: ... VA) were cultured at 37 °C and 5% CO2 in DMEM-HG (Life Technologies, Carlsbad, CA) supplemented with 10% FBS (Clontech ...
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bioRxiv - Biophysics 2022Quote: ... labeled for 30 min with 5 µM Alexa FluorTM 488 C5 maleimide (Thermo Fisher Scientific, A10254) in high potassium KD98 solution (98 mM KCl ...
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bioRxiv - Bioengineering 2022Quote: ... RBC (5 million cells/mL) were incubated with 2 μg/mL calcein AM (Thermo Fisher Scientific) for 15 minutes at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... VOs we’re fixed for 1 hour in 4% paraformaldehyde solution (Thermo Fisher Scientific, 7732-18-5) in PBS and for 2 hours in 2.5% glutaraldehyde in PHEM buffer (TAAB ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged approximately every 3-5 days (70-85% confluency) using Accutase (Gibco, #11105-01) to detach cells ...
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bioRxiv - Cancer Biology 2024Quote: ... plasmid containing a sgRNA targeting APC (5‘-GGCACTCAAAACGCTTTTGA-3‘) using lipofectamin-2000 (Thermo Fisher Scientific, 11668027). WT organoids were grown in WENR medium and APC truncated organoids were grown in ENR medium and are later refererred to as mCol WT and mCol APC ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 ug of empty vector or FLAG-POLR3B was transfected per well using lipofectamine 2000 (Invitrogen). After 24h ...
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bioRxiv - Molecular Biology 2024Quote: Reverse siRNA transfections were done using 5 nM Silencer Select pre-designed siRNAs (Thermo Fisher Scientific) with Lipofectamine RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were grown at 37°C in a 5% CO2 atmosphere in DMEM (Gibco 11995065), supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2024Quote: ... qRT-PCR was then performed using the QuantStudio 5 Real-Time PCR system (Thermo Fisher Scientific). ΔCt values were calculated using GAPDH and compared between treatment groups ...
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bioRxiv - Bioengineering 2024Quote: ... IVT was then performed using MEGAscript® T7 Transcription Kit (Thermo Fisher Scientific, AMB-1334-5) according to the manufacturer’s instructions with modifications as using the CleanCap® Reagent AG (TriLink ...
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bioRxiv - Microbiology 2024Quote: ... 5 μl of DNase I buffer (Fermentas) and 0.5 μl of Superase In (Thermo Fisher Scientific) were added to the denatured RNA and incubated at 37°C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Nucleus were stained for 5 min with Hoechst n°33342 (ref H3570, Invitrogen, Carlsbad, California, USA) at 1:2000 ...