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Citations for Thermo Fisher :
7051 - 7100 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... the cells were seeded on a 6-well plate with 2 ml of DMEM medium supplemented with 5% horse serum (Gibco). At 24 hours after transfection ...
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bioRxiv - Cancer Biology 2019Quote: The human myeloma cell line KJON (19) was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR was conducted using a 5’ fluorescently-labelled primer (6-FAM, PET, NED, or VIC) (Applied Biosystems, Foster City, CA) and an unlabeled reverse primer ...
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bioRxiv - Neuroscience 2021Quote: ... Longitudinal imaging of vascular leakage in C57BL/6 mouse was performed for 5 days after injecting 600 μg of 3kDa TRITC-dextran (LIF-D-3308, Invitrogen) and 600 μg of 2MDa FITC-dextran (LIF-D-7137 ...
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bioRxiv - Genomics 2020Quote: ... cells were harvested and grown for 5-6 passages in NPC expansion medium (selection medium with the addition of 2% B27 [GIBCO] ...
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bioRxiv - Developmental Biology 2020Quote: Neural retinae were dissected from adult zebrafish dark adapted for 12 hours between 5:30 PM and 6:30 PM and stored in RNAlater (Ambion) at 4°C until extraction ...
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bioRxiv - Microbiology 2021Quote: ... volvulus worm to each well of 6-well plates containing 5 ml of DMEM +10% fetal bovine serum (Gibco, A3840002) + 100 u/ml penicillin + 100 ug/ml streptomycin + sodium bicarbonate (2 g/1) ...
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bioRxiv - Cell Biology 2020Quote: ... The production of ROS was assessed by the oxidation of 5-6-chloromethyl-2,7-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) (Invitrogen Corporation). Cells cultured in 96-well plates were incubated with JC1 ...
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bioRxiv - Cell Biology 2021Quote: ... 1-2 μg of plasmid was used to transfect 500,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5-10 μL; Thermo Fisher) in OptiMEM (400 μL ...
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bioRxiv - Neuroscience 2022Quote: ... Ss06901209_m1), fibulin-5 (FBLN5, Ss04805024_m11) and samples were run on a QuantStudio 6 Real-Time PCR system (Applied Biosystems #4485694) in technical and biological replicates (n = 3-5) ...
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bioRxiv - Biophysics 2022Quote: Fibroblasts grown in vitro to the third passage were plated in 6-well tissue culture plates (5 × 105 cells per well) in DMEM (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Cell Biology 2023Quote: ... which were blotted with a force of 6 for 5 seconds and immediately vitrified by plunge-freezing in liquid ethane using a Vitrobot Mark IV (ThermoFisher) operating at 4 °C and 100% humidity ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg of plasmid was used to transfect 750,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 µL; Thermo Fisher) in OptiMEM (400 µL ...
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bioRxiv - Cancer Biology 2023Quote: Rhodamine tubulin was generated by labeling tubulin with X-rhodamine SE (5(and-6) carboxy-X-rhodamine succinimidyl ester) dye (ThermoFisher). Labeling stoichiometry was determined using a molar extinction coefficient of 115,000 M-1 cm-1 for tubulin and 78,000 M-1 cm-1 for X-rhodamine [58] ...
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bioRxiv - Cancer Biology 2024Quote: ... 5E6 (5 × 10^6) GSU-Redfluc cells were suspended in 100 uL DPBS (Dulbecco’s Phosphate Buffered Saline; Gibco Inc., USA) and xenografted subcutaneously into NSG mice ...
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bioRxiv - Pathology 2019Quote: DBA/2-PMLECs were plated in a Lab-Tek chamber slide system made of Permanox containing 8 wells (3 ×104 cells/well) (Thermo Fisher Scientific - 177 455). Afterwards ...
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bioRxiv - Microbiology 2019Quote: ... Samples were mixed with an equal volume of NuPAGE® Tris-glycine native sample buffer (Novex®) and loaded on to NuPAGE™ 3-8% Tris-acetate gel (Thermofisher, USA). Gel blots were run at 150 V for 3 hours ...
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bioRxiv - Neuroscience 2019Quote: 3 × 104 A4 and untransfected SH-SY5Y cells were seeded into 8-well Lab-Tek®II Chamber Slides (Nalge Nunc, Naperville, IL, U.S.A) and left for 48 h to strongly adhere ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 µg of protein was mixed with NuPAGE sample reducing agent and NuPAGE LDS sample buffer and run on NuPAGE 3 to 8% Tris-Acetate gel (all Thermo Fisher Scientific, Abingdon, UK). Protein was electrotransferred onto a 0.45 µm polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2020Quote: MEF R1441C cells were plated on 8-well dishes at 3×104 cells per each well (Nunc™ Lab-Tek™ II Chambered Coverglass from Thermo Fisher Scientific). Cells were cultured overnight with complete media including 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... The filtered supernatants were concentrated 8-fold using a pre-rinsed 3K MWCO Pierce Protein Concentrator PES (molecular-weight cutoff: 3 kDa) (Thermo Fisher Scientific, 88525, USA) by centrifuging at 4000 rcf in 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were washed 3 times with PBS and were mounted on the slides using Fluorescent-G mounting medium (Invitrogen, Carlsbad, CA). Final concentrations of primary Abs were used as follows ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was then centrifuged at 3.9 k x g for 15 minutes and concentrated using a Pierce™ protein concentrator with a 3 kilodalton cut-off (ThermoFisher, 88526). The concentrated medium was diluted 1:5 with synthetic seawater before use ...
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bioRxiv - Neuroscience 2024Quote: ... tissue was washed in DMEM with GlutaMAXTM-I and pelleted again (300 x g, 3 min, RT) before trituration in culture media (neurobasal media, # 21103-049, Thermo Fisher Scientific) with 100 U/ml penicillin ...
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bioRxiv - Microbiology 2020Quote: ... 100μm lung slices were washed twice in 200 mM HEPES buffer then incubated for 20 min in a staining solution containing: 0.715 μM DAPI (4′,6-diamidino-2-phenylindole) (ThermoFisher Scientific D1306), 10 mg/L BODIPY 493/503 (4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 and 6 were directly cloned into pCR(tm)2.1-TOPO® using the TOPO® TA Cloning® Kit (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting suspension with all nuclei was stained with the fluorescent DNA intercalant marker DAPI (4’-6-diamidino-2-phenylindole dihydrochloride, Invitrogen, USA) diluted 1:20 from a stock solution of 20 mg/L and filled with PBS 0.1M to known volume ...
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bioRxiv - Zoology 2020Quote: ... Tissues were cut into small pieces with a scalpel and washed twice in ADPBS containing antibiotics prior to placing 4-6 tissue pieces into a 25 cm2 plug-seal tissue culture treated flask (BioLite, Thermo Scientific) containing 1.5 mL establishment medium to cover the tissue pieces ...
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bioRxiv - Biophysics 2021Quote: ... cells were transiently transfected with 4 μg mouse Piezo1-pIRES-EGFP in pcDNA3.1(+) or 4 μg YFP 40-48 hours before recording in 6-well plates using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... 4 µg of plasmid was transfected with 4 µL P3000 regent and 6 µL Lipofectamine 3000 as per scaled recommendations in the manufacturer’s protocol (Thermo Fisher Scientific). Medium containing lipid complexes was removed 4 h post transfection and replaced with exosome-depleted phenol red-free DMEM + 10% FBS + 2 mM L-glutamine (Thermo Fisher) ...
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bioRxiv - Bioengineering 2021Quote: ... 4 µg of plasmid was transfected with 4 µL P3000 regent and 6 µL Lipofectamine 3000 as per scaled recommendations in the manufacturer’s protocol (Thermo Fisher Scientific). Medium containing lipid complexes was removed 4 h post transfection and replaced with exosome-depleted phenol-free DMEM + 10% FBS + 2 mM L-glutamine ...
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bioRxiv - Bioengineering 2021Quote: ... and the cells were incubated for 50 minutes and DAPI (4′,6′-diamino-2-phenylindole) (Molecular Probes, Thermo Fisher Scientific, USA) for 5 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... and the cells were incubated for 50 minutes and DAPI (4′,6′-diamino-2-phenylindole) (Molecular Probes, Thermo Fisher Scientific, USA) for 5 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... slides were washed 3 x 5 min with PBS-TX and once with 0.1 M phosphate buffer pH 7.4 (0.08 M sodium phosphate dibasic and 0.02 M sodium phosphate monobasic) prior to coverslipping with ProLong Gold antifade reagent with 4’,6-diamidino-2-phenylindole [DAPI] (Invitrogen, P36931).
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bioRxiv - Neuroscience 2021Quote: Hair cell nuclei were specifically labeled by briefly exposing free-swimming larvae to 4’,6-Diamidino-2-Phenylindole (DAPI; Invitrogen/ThermoFisher) diluted to 2.5 μg/ml in E3 ...
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bioRxiv - Genetics 2021Quote: ... cells were washed and suspended in flow cytometry buffer containing 1µg/ml 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2020Quote: ... Sections were cut at 4-6 μm and dried from a drop of dH2O onto Probe On Plus slides (ThermoFisher Scientific). Tissues sections were stained with 0.01% aqueous Toluidine Blue O ...
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bioRxiv - Molecular Biology 2020Quote: ... once with 1X SSC at room temperature for 15 min and twice with 0.5X SSC at room temperature for 15 min and finally with 1X PBS buffer containing 0.5 µg/mL 4′,6-diamidino-2-phenylindole (DAPI) and mounted onto ProLong Gold antifade reagent (Thermo Fisher Scientific) mounting media.
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bioRxiv - Cell Biology 2022Quote: ... the cerebral cortices of 4 to 6 P0 rats were dissociated and chopped in Ca++/Mg++-free Hank’s Balanced Salt Solution (HBSS; Invitrogen, Carlsbad, CA). The tissue pieces were dissociated in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ... diluted 1:200 in blocking buffer for 30 min and mounted with ProLong Gold Antifade with DAPI (4′,6-diamidino-2- phenylindole; Thermo Fisher). Confocal laser micrographs were obtained with Zeiss LSM 880 laser microscope and analyzed with Zen black and Fiji software ...
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bioRxiv - Biophysics 2022Quote: ... gels were functionalized with 0.5 mg/ml solution of sulfosuccinimidyl 6-(4′-azido-2′-nitrophenylamino) hexanoate (Sulfo-SANPAH) (Thermo Fisher Scientific) prepared in 50 mM HEPES buffer (Santa Cruz Biotechnologies) ...
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bioRxiv - Microbiology 2020Quote: ... cells were extensively washed and incubated with secondary antibodies in the presence of 4′,6-diamidino-2-phenylindole (DAPI, Molecular Probes) for 45 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed and mounted in media (0.5% N propyl gallate, 70% glycerol, 1M tris pH8.0) containing 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Cat. No. D1306).
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bioRxiv - Microbiology 2022Quote: ... After extensive washing slides were air-dried and mounted in Gold Antifade containing 4’,6-diamidino-2-phenylindole (DAPI) (P3693; Life Technologies) for imaging ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed in PBS-T and mounted under Prolong gold fluorescence media with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, P36931).
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bioRxiv - Neuroscience 2019Quote: ... The slices were incubated separately in wells with PBS and 5 ng/ml DAPI (4′,6-diamidino-2-phenylindole) for 20 min before they were rinsed in PBS and mounted in SlowFade Gold (Life Technologies). Superficial cortical areas of anterior and midcingulate cortex were imaged on a Leica SP8 inverted confocal microscope with a 63x oil immersion objective (numerical aperture = 1.4 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Alexa Fluor 488–conjugated phalloidin (#A12379) and 4’,6-diamidino-2-phenylindole (DAPI) (#1306) were obtained from Invitrogen (Carlsbad, CA, USA). IRDye800CW-labeled anti-rabbit secondary antibody (#926-32211 ...
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bioRxiv - Neuroscience 2020Quote: ... slides were washed and stained with 4′,6-Diamidin-2-phenylindol (DAPI, 5μg/ml) before mounting coverslips with ProlongTM Gold Antifade reagent (Thermo Fisher Scientific). Images were acquired using a LSM 710 confocal microscope (Zeiss ...
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bioRxiv - Microbiology 2021Quote: ... samples were unfrozen and aliquots of 6 ml per sample were fixed with Electron Microscopy Sciences 4% Paraformaldehyde (5025999, Fisher Scientific), stained with SYBR™ Gold Nucleic Acid Gel Stain (S11494 ...
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bioRxiv - Immunology 2021Quote: ... The isolated nuclei were centrifuged at 500 x g for 5 min at 4°C and respectively resuspended with Nuclei wash buffer stained with 10 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) before FACS (5mg/ml, Invitrogen D1306). After sorting using purity mode ...