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Citations for Thermo Fisher :
7301 - 7350 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Sections were first incubated overnight at 4°C in rabbit anti-CALR (1:500, ThermoFisher, Waltham, MA, cat# PA3900) then goat anti-rabbit Alexa Fluor 488 (1:2000 ...
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bioRxiv - Immunology 2019Quote: ... loaded onto NuPAGE® 4-12% gels and run at 120 V in 1 X MES buffer (ThermoFisher Scientific). The separated proteins were transferred onto membranes using the iBlot® transfer system (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... at 4 °C followed by incubation the next day with goat anti-chicken antibody (647nm, A21449, ThermoFisher, 1:600) and goat anti-rabbit antibody (568 nm ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated with primary antibodies (in 0.01 M PBS with 1% BSA and 0.3% Triton X-100) overnight at 4 °C and subsequently with corresponding secondary antibodies (1:1,000, Alexa-Fluor-conjugated, Invitrogen). DAPI was used to label the nuclei and sections were mounted with 75% glycerol ...
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bioRxiv - Biophysics 2020Quote: ... cells were previously fixed with paraformaldehyde 4% and permeabilized with Triton-X100 (0.5%) in PBS and stained with 1/200 anti-E-cadherin-FITC (Invitrogen).
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bioRxiv - Bioengineering 2020Quote: ... 1×1010 sEV were incubated with 3.8 µm aldehyde/sulfate latex 4% (m/v) beads (Molecular probes, Eugene, OR). Coated beads were then incubated with anti-human CD63 (eBioscience ...
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bioRxiv - Bioengineering 2021Quote: ... cells were live-dead stained with 2 µM calcein acetoxymethyl and 4 µM ethidium homodimer-1 (#L3224, Thermo Fisher) and imaged using fluorescent microscopy to compare cell density and viability between each silicone substrate ...
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bioRxiv - Bioengineering 2020Quote: ... 1-mm coronal sections from each tissue (4-mm for the brain) were homogenized in Trizol (Life Technologies, 15596) using a BeadBug (Benchmark Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... 2 µg PAX2 and 4 µg shRNA in pLKO.1 backbone using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated overnight at 4 °C with primary antibody against ZO-1 (7.5 μg/ml, Thermo Fisher Scientific). Alternatively ...
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bioRxiv - Microbiology 2021Quote: ... AGS cells were transiently transfected for 24 h with the sgRNA-containing pX459 vector using polyethylenimine (PEI) at a 4:1 PEI:DNA ratio in Opti-MEM I Reduced Serum Media (OptiMEM; Gibco). Puromycin treatment (2 µg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... The organoids were incubated overnight at 4◦C with a primary antibody against KI67 (Invitrogen #MA5-14520; 1:200) or MUC2 (Santa Cruz #sc-15334 ...
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bioRxiv - Neuroscience 2020Quote: ... TRA-1-60) in iPSCs was performed using the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit (Invitrogen™, A24881). iPSC colonies were fixed for 15 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... islets were similarly incubated for 1 hr in imaging buffer at 37 °C with the addition of 2.5 μM Fluo-4 (Invitrogen). Islets incubated with Fluo-4 were washed three times with imaging buffer prior to imaging ...
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bioRxiv - Microbiology 2022Quote: ... lipid concentrations of FtsA fractions were determined by incubation of FtsA with FM 4-64FX (1.25 μg ml-1) (Invitrogen). Fluorescence intensity was measured with an Agilent Eclipse spectrofluorometer using an excitation wavelength of 565 nm and an emission wavelength of 745 nm (slit widths 5/10 nm) ...
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bioRxiv - Biochemistry 2022Quote: ... Sections were incubated overnight at 4 degrees with anti-ATF4 (10835-1-AP, ThermoFisher, Waltham, MA USA, RRID: AB_2058600) antibody at a 1:200 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... the media in each plate was replaced with 40 μL of 1× Hank’s Balanced Salt Solution (HBSS) containing 20 mM HEPES pH 7.4 and 4× dilution of Fluo4 (Thermo Fisher). The assay plate was incubated at 37 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... 10% dialyzed fetal bovine serum (PAA),4 mM L-glutamine (PAA),1% penicillin/streptomycin (100 IU/100 μg, Invitrogen). For Light Medium ...
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bioRxiv - Molecular Biology 2020Quote: ... Crosslinking was reversed by incubating the beads at 65°C for 4 hr in the presence of 0.3% SDS and 1 mg/ml of Proteinase K (Ambion). DNA was purified using Zymo ChIP DNA clean and concentrator kit (Zymo Research ...
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bioRxiv - Microbiology 2020Quote: ... 4 x TaqMan® Fast Virus 1-Step Master Mix made up with molecular-grade nuclease free water (Ambion) to a final volume of 15 μl ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then incubated overnight at 4°C with a 1:500 dilution of rabbit anti-HA antibody (Invitrogen) in PBS 1% BSA ...
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bioRxiv - Microbiology 2021Quote: ... 6.25 µl of 4 μM primers P1 and P2 and 1 µl of template DNA (Thermo Scientific, cat#F553S), and milliQ water up to 50 μl ...
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bioRxiv - Microbiology 2020Quote: ... BCR-expressing Ramos B cells were suspended at 4 million cells/mL in RPMI++ and labeled with 1.5 μM Indo-1 (Invitrogen) for 30 min at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... falciparum NF54 parasites were cultivated at 4% hematocrit in human B+ blood in RPMI-HEPES supplemented with 0.25% Albumax 1 (Invitrogen) and human donor plasma type B+ ...
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bioRxiv - Cancer Biology 2020Quote: ... overnight at 4°C and with anti-rabbit IgG Alex Fluor 633 (ThermoFisher, Cat# A-21070, dilution 1:2000) for one hour at room temperature ...
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Mutation in the matricellular gene fibulin-4 leads to endothelial dysfunction in resistance arteriesbioRxiv - Physiology 2022Quote: ... Vessels were then fixed in 4% paraformaldehyde for 10 min and buffer containing DAPI (1:2,000, Thermofisher, Waltham, MA) for 5 min ...
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bioRxiv - Cell Biology 2019Quote: ... the viral medium was them replaced with induction medium composed of DMEM/199 (4:1) (Gibco, Thermo Fisher Scientific) containing 10% FBS ...
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bioRxiv - Cell Biology 2019Quote: ... the viral medium was them replaced with induction medium composed of DMEM/199 (4:1) (Gibco, Thermo Fisher Scientific) containing 10% FBS ...
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bioRxiv - Neuroscience 2019Quote: Tissue from anonymized donors was received at 4°C in 1 ml “fibroblast medium” containing MEM alpha (Gibco, #12571), 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were washed 4 x 10 minutes in PBS-Tr at room temperature with DAPI (1:5,000, Invitrogen D1306) added during the third wash ...
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bioRxiv - Biochemistry 2021Quote: ... and complemented with 7.5 μM of Site2hp RNA (4:1 protein:RNA molar ratio) in PBS buffer in a 384 black-well clear-bottom plate (Nunc). A ThT stock was prepared as described above and added to the well to 300 μM final concentration ...
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bioRxiv - Cell Biology 2021Quote: ... first dimensional lanes are cut from the gel and incubated in 1% SDS with 1% beta-mercaptoethanol for 1 hour before the gel slice was loaded into an NuPAGE 4-13% 1.0mmx2D gel (Invitrogen). Gels were run at 200V for 50 minutes at room temperature in MOPS ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with primary antibodies (anti-ACE2 (1:1000; MA5-32307 - Thermo Fisher), anti-Sigma-1 R (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... at 4°C for 1hr and 45 minutes in 1X NuPAGE® Transfer Buffer (Life Technologies, cat# NP0006-1). Blocking and antibody incubation was performed in 2% BSA (Sigma Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated overnight at 4°C with rabbit anti-ApoE antibody (1:200, #701241, Invitrogen, Carlsbad, CA, USA) and either rat anti-CD31 antibody (1:200 ...
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bioRxiv - Immunology 2020Quote: ... Baker) for 45 min at 4 °C and then permeabilized with either 1 × Permeabilization Buffer (transcriptional factor staining, ThermoFisher) or 1 × Perm/Wash Buffer (cytokine staining ...
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bioRxiv - Immunology 2021Quote: ... Blots were incubated overnight at 4°C with agitation in blocking buffer and anti-HisG-HRP (1:5000, Invitrogen), or anti-V5-HRP (1:5000 ...
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bioRxiv - Immunology 2021Quote: ... CCR10 (248918) from R&D, CD11a (M17/4) from eBioscience, FcεRI (MAR-1), c-Kit (CD117, 2B8) from Invitrogen and Mouse BD Fc Block™ ...
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bioRxiv - Cancer Biology 2020Quote: ... overnight at 4°C and with anti-rat IgG Alex Fluor 488 (ThermoFisher, Cat# A-11006, dilution 1:1000), anti-guinea pig IgG Alex Fluor 568 (ThermoFisher ...
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bioRxiv - Bioengineering 2022Quote: ... within a biosafety cabinet SIS was cut into 1-inch segments and then incubated in 4 % ethanol (Fisher Scientific) and 0.1 % peracetic acid (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... thinly sliced (1-2 mm) tissue samples were fixed overnight at 4°C in neutral-buffered formalin (Fisher Scientific) with PhosSTOP added (Roche) ...
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bioRxiv - Bioengineering 2022Quote: ... 1:100 overnight at 4°C followed by Alexa Fluor 594 goat-anti rabbit secondary antibodies (Invitrogen, ON, Canada) at 1:400 for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... tissue was digested for a further 24 h with 0.5 mg/mL collagenase type IV (CLS-4, Lorne Laboratories) and 1 mg/mL dispase II (17105041, Invitrogen) at 37 °C and 5% CO2 with constant agitation [38].
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The muscle cells were centrifuged at 300g for 5 minutes and the cell pellet resuspended in Ham’s Complete growth medium (4% bovine calf serum [cytiva, Cat# SH30077.03], 1% penicillin and streptomycin [gibco by Life Technologies, Cat# 15140122] ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were incubated overnight at 4 °C with specific anti-FASN (#PA5-22061, Thermo Fisher Scientific; dilution 1:400) and anti-LAMP2 (#H4B4 ...
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bioRxiv - Microbiology 2023Quote: Cultured viruses were collected by centrifugation at 9,000 rpm for 1 h at 4°C (Sorvall ST8FR, Thermo Scientific) and resuspended in phosphate-buffered saline ...
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bioRxiv - Neuroscience 2022Quote: ... overnight at 4 °C followed by detection with Alexa 488 conjugated anti-rat secondary antibody (1:1000, Life Technologies). Arteries were identified by immunostaining with a mouse monoclonal anti actin ...
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bioRxiv - Plant Biology 2022Quote: ... and qualified and quantified using 1% agarose gel and a Qubit™ Fluorometer 4 (Invitrogen, Life Technology, CA, USA). DNA was randomly fragmented using Bioruptor® Pico B01060010 (Diagenode ...
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bioRxiv - Neuroscience 2024Quote: ... confluent iPSC cultures were detached using 0.5mM EDTA and plated 1:4 on Matrigel ES-coated plates in StemFlex Medium (Gibco) supplemented with 10μM ROCK inhibitor (SellChem) ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were then incubated at 4°C for 18 hours with secondary antibodies: DAPI (D1306, Life Technologies, 1:1000), AF488 (A11008 ...