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Citations for Thermo Fisher :
6901 - 6950 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... pH=8 (ThermoFisher, MA, USA) for a total volume of 100 μL and sonicated (4 cycles of 20sec/30sec (on/off time) ...
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bioRxiv - Cell Biology 2024Quote: ... Essential 8™ medium (Gibco) containing 100 IU/mL penicillin and 100 µg/mL streptomycin was used for cell growth and renewed daily ...
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bioRxiv - Neuroscience 2023Quote: ... 8 ng/ml bFGF (Gibco), 1xGlutamax (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... 8 ng/ml bFGF (Gibco), 1x Glutamax (Gibco) ...
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bioRxiv - Systems Biology 2024Quote: ... 8 mg/mL collagenase (Gibco) in HBSS/4 mM CaCl2 (Fisher) ...
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bioRxiv - Molecular Biology 2019Quote: ... G-MEM (Gibco) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... Fluoromount-G (Invitrogen) was used to prepare the coverslips for cell imaging ...
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bioRxiv - Neuroscience 2023Quote: ... Fluoromount-G (Invitrogen) containing DAPI was used for mounting IHC stained slices ...
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bioRxiv - Neuroscience 2023Quote: ... Fluoromount-G (Invitrogen) was used to mount coverslips (Vetrini Coprioggetto ...
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bioRxiv - Microbiology 2020Quote: ... CHIKV was labeled with the lipophilic fluorescent probe DiD (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt; Life Technologies), as described previously [5] ...
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bioRxiv - Genomics 2019Quote: ... Primary antibodies (anti-human CD47-APC; clone CC2C6, BioLegend, Cat. #323123/4, RRID: AB_2716202/3; and clone B6H12.2, ThermoFisher Scientific ...
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bioRxiv - Genetics 2020Quote: mRNA was collected from groups of 10 whole larvae (n=3–4 replicates per line) using Trizol (ThermoFisher) and reverse transcribed to cDNA using SuperScript III Reverse Transcriptase (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... and Antibiotic-Antimycotic Mixed Stock Solution (Nacalai) and were passaged every 3-4 days with TryPLE (ThermoFisher Scientific). For microscopy observations ...
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bioRxiv - Bioengineering 2020Quote: ... Bodipy FL c16 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic Acid, Thermofisher) was diluted in sterile RPMI-1640 (Corning ...
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bioRxiv - Molecular Biology 2020Quote: ... a sample of eluates (3%) was separated on acrylamide NuPAGE Novex 4–12% Bis-Tris gels (Life Technologies) and analyzed by silver staining.
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bioRxiv - Molecular Biology 2019Quote: ... Cells were passaged every 3-4 days with 0.25% trypsin-EDTA solution (Life Technologies, cat. no. 25200-056) and washed with sterile PBS (Life Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... and then diluted into fresh YPD for 3-4 hours before inoculating into 96-well plates (Thermo Scientific) at a starting OD600 between 0.04 to 0.1 ...
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bioRxiv - Cell Biology 2024Quote: ... and a lentiviral transfer plasmid (2:3:4 ratio by mass) using Lipofectamine 3000 (Thermo Fisher Scientific L3000015). Viral supernatant was harvested 48h after transfection and filtered through 0.45 µm cellulose acetate filters (Corning 431220) ...
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bioRxiv - Cancer Biology 2024Quote: ... at 95⁰C for 3 min and loaded onto a SDS-polyacrylamide gel (4-12%, Invitrogen NuPAGE, #NP0336BOX). After size separation ...
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bioRxiv - Biochemistry 2023Quote: ... the filtered supernatant was loaded onto 3-4 mL immobilized D-galactose gel (Pierce™, Thermo Scientific™) equilibrated by gravity flow with Gal A buffer (50 mM Na-phosphate pH 7.4 ...
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bioRxiv - Immunology 2024Quote: 50 000 enriched CD11b+ LCs were seeded and incubated for 10min at 37°C with 2mM Bodipy-C11 (581/591) (4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid; Invitrogen) in PBS ...
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bioRxiv - Genomics 2024Quote: ... The remaining cells are resuspended in our working cell buffer solution (AMES/0.4% BSA + 3 µM ActD) and incubated with CD90.2/Thy1.2 antibody conjugated to APC (#17-0902-83; Invitrogen, Carlsbad ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by dissociation with Accutase™ (37°C, 5 mins) and thereafter FACs sorted for viable cells using 7-AAD staining (Thermo Fisher Scientific, #A1310).
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bioRxiv - Microbiology 2022Quote: ... and gene-specific primers were used to perform quantitative reverse transcription-PCR (qRT-PCR) on 5 ng/μL cDNA in a Real-Time PCR System (Applied Biosystems ViiA 7, USA). Table S2 lists the primers used in qRT-PCR ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μl of reverse transcription mix (50 mM Tris-HCl, pH 8.3 [Sigma], 75 mM NaCl [Ambion], 1 mM GTP [Thermo Scientific], 3 mM MgCl2 [Ambion] ...
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bioRxiv - Immunology 2021Quote: ... for 3 days and cultured in RPMI 1640 medium supplemented with 10% of FBS and 1% penicillin/streptomycin (all Gibco, Thermofisher). To obtain monocytes ...
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bioRxiv - Developmental Biology 2021Quote: ... and in secondary antibody at 1:500 for 3 hours at room temperature goat (anti-rabbit Alexa Fluor 488, cat#A11008 Invitrogen). Sections were mounted using Vectashield with DAPI.
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bioRxiv - Microbiology 2019Quote: ... 8 x 10^5 HEK-293T/17 cells pre-plated in a 6-well plate were co-transfected with a mass ratio of 1:3 replicon:C-prM-E plasmids using Lipofectamine 3000 (Cat# L3000-015; ThermoFisher Scientific). Four hours post-transfection ...
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bioRxiv - Developmental Biology 2021Quote: ... and 4.5 µl of RfxCas13d protein (3 mg/ml) were mixed in 1 µl of nuclease-free water with phenol red (Invitrogen). One to 5 nL of RNPs were injected into one- to two-cell stage Ancistrus sp ...
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bioRxiv - Evolutionary Biology 2021Quote: ... an antisense digoxigenin-labeled riboprobe against Nematostella vectensis polycystin 1 was synthesized by using 3’ RACE product as a template (MEGAscript transcription kit; Ambion), and was used at a final concentration of 1 ng/μl ...
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bioRxiv - Biophysics 2022Quote: ... The samples were rinsed with pre-cold 1xPBS 3 times and incubated with the secondary antibody Alexa 594-labeled goat anti-rabbit (1:800, Cat# A11037, Invitrogen) at RT for 90 min ...
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bioRxiv - Cell Biology 2022Quote: ... The monolayers were washed 3 times with PBS and stained with Alexa Flour 647 conjugated with goat anti mouse IgG (1:2,000, ThermoFisher, A32728) and Alexa Flour 594 conjugated with donkey anti rabbit IgG (1:1,000 ...
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bioRxiv - Bioengineering 2019Quote: ... rinsed 3 times in HBSS buffer and incubated with Alexa Fluor 488 WGA (1:200, A12379, Life Technologies, Zug, Switzerland) for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... We then diluted these saturated cultures 1:20 in sterile water and then inoculated 3 µL into 96-well humidified plates (Nunc Edge Plates (Thermo Scientific ...
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bioRxiv - Microbiology 2019Quote: ... Coverslips were incubated with anti-FLAG M2 mouse monoclonal antibody at a dilution of 1:500 for 3 hours at room temperature and secondary antibody conjugated to fluorophore at a dilution of 1:1000 (Invitrogen). 45 images per coverslip were obtained with a Nikon Eclipse TE2000-E pinning disc confocal microscope equipped with an Andor laser system and Borealis beam conditioning unit ...
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bioRxiv - Microbiology 2021Quote: ... The 5’ and 3’ fragments (2 μg of each) were ligated for 1 h at 16°C by T4 DNA ligase (Invitrogen) and purified by phenol-chloroform extraction ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Wells were quickly washed once with 0.25% trypsin (by pipetting up and down 3 times) and twice with PBS to remove 3T3 cells before collecting keratinocytes into 1 ml Trizol (Invitrogen). Details of the samples used for polyI:C challenge across the 24 wolf keratinocyte cultures are provided in Supplementary Table S1.
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bioRxiv - Immunology 2021Quote: ... Samples were washed 3 times with PBS-T and then 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific) was added for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... sections were rested at RT for 30 minutes before washing thrice with PBS for 3 minutes each and incubating with 1:200 goat anti-rabbit IgG-AlexaFluor647 (A21245 - Invitrogen) for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... The plate was washed 3 times before adding 50 μl of 1:1,000 diluted Goat anti-Mouse IgG H+L-HRP antibody (Invitrogen, # 31430) to the plate and incubated for 1 h at RT ...
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bioRxiv - Bioengineering 2020Quote: ... hPSCs were washed 3 times with PBS and dissociated into single cells by incubation in 1 mL of TrypLE Express (GIBCO) at 37°C for 3 min ...
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All-trans retinoic acid induces synaptopodin-dependent metaplasticity in mouse dentate granule cellsbioRxiv - Neuroscience 2021Quote: ... sections were incubated for 3 hours with streptavidin-Alexa Fluor 488 (Invitrogen, #S32354; 1:1000 dilution in 10% (v/v) NGS ...
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bioRxiv - Neuroscience 2020Quote: ... for 48 h then transfected for 24 h with 1 ug His-G3BP1 3’UTR constructs using Lipofectamine LTX and Plus reagent (Invitrogen). To determine mRNA stability ...
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bioRxiv - Neuroscience 2020Quote: ... after 3 washes with PBS cells were incubated with secondary antibody (Alexa Fluor conjugated antibody, 1:500, Molecular probes, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... after 3 washes with PBS cells were incubated with secondary antibody (Alexa Fluor conjugated antibody, 1:500, Molecular probes, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 100 ng of yellow fluorescent protein (YFP) cDNAs in each well using 1:3 DNA: Lipofectamine 2000 (11668019, Invitrogen) ratio ...
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bioRxiv - Cell Biology 2021Quote: ... the tissue was dissolved in 3 ml freshly prepared primary dissociation buffer (collagenase 1 mg/ml, Sigma C6885, in DMEM w/o phenol red, Gibco) and passed through a cut p1000 pipet tip ten times or until smooth passing ...
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bioRxiv - Cancer Biology 2021Quote: ... were evenly coated with 75 µL of a 1 mg/mL collagen solution composed of 3 mg/mL rat tail collagen (Gibco), serum free media ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then passaged 1:3 as clumps using 15 min 37°C incubation with 1mg/ml collagenase IV (Invitrogen) onto plates coated with 1:50 GFR-Matrigel (Corning ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were washed 3 more times in 1x PBS before DNA detection with 1 µg/ml Hoechst 33342 (Invitrogen, H3570) for 15 min at room temperature ...